TY  - JOUR
A1  - Malinova, Irina
A1  - Kössler, Stella
A1  - Orawetz, Tom
A1  - Matthes, Ulrike
A1  - Orzechowski, Slawomir
A1  - Koch, Anke
A1  - Fettke, Jörg
T1  - Identification of two Arabidopsis thaliana plasma membrane transporters able to transport glucose 1-phosphate
JF  - Plant & cell physiology
N2  - Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidences have shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. In this study, we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal an antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.
KW  - apoplast
KW  - Arabidopsis thaliana
KW  - glucose 1-phosphate transport
KW  - starch metabolism
KW  - sugar transport
Y1  - 2020
U6  - https://doi.org/10.1093/pcp/pcz206
SN  - 0032-0781
SN  - 1471-9053
VL  - 61
IS  - 2
SP  - 381
EP  - 392
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Zhang, Yunming
A1  - Ramming, Anna
A1  - Heinke, Lisa
A1  - Altschmied, Lothar
A1  - Slotkin, R. Keith
A1  - Becker, Jörg D.
A1  - Kappel, Christian
A1  - Lenhard, Michael
T1  - The poly(A) polymerase PAPS1 interacts with the RNA-directed DNA-methylation pathway in sporophyte and pollen development
JF  - The plant journal
N2  - RNA-based processes play key roles in the regulation of eukaryotic gene expression. This includes both the processing of pre-mRNAs into mature mRNAs ready for translation and RNA-based silencing processes, such as RNA-directed DNA methylation (RdDM). Polyadenylation of pre-mRNAs is one important step in their processing and is carried out by three functionally specialized canonical nuclear poly(A) polymerases in Arabidopsis thaliana. Null mutations in one of these, termed PAPS1, result in a male gametophytic defect. Using a fluorescence-labelling strategy, we have characterized this defect in more detail using RNA and small-RNA sequencing. In addition to global defects in the expression of pollen-differentiation genes, paps1 null-mutant pollen shows a strong overaccumulation of transposable element (TE) transcripts, yet a depletion of 21- and particularly 24-nucleotide-long short interfering RNAs (siRNAs) and microRNAs (miRNAs) targeting the corresponding TEs. Double-mutant analyses support a specific functional interaction between PAPS1 and components of the RdDM pathway, as evident from strong synergistic phenotypes in mutant combinations involving paps1, but not paps2 paps4, mutations. In particular, the double-mutant of paps1 and rna-dependent rna polymerase 6 (rdr6) shows a synergistic developmental phenotype disrupting the formation of the transmitting tract in the female gynoecium. Thus, our findings in A. thaliana uncover a potentially general link between canonical poly(A) polymerases as components of mRNA processing and RdDM, reflecting an analogous interaction in fission yeast.
KW  - poly(A) polymerase
KW  - RNA-directed DNA methylation
KW  - pollen development
KW  - siRNAs
KW  - transposable elements
KW  - gynoecium development
KW  - Arabidopsis thaliana
Y1  - 2019
U6  - https://doi.org/10.1111/tpj.14348
SN  - 0960-7412
SN  - 1365-313X
VL  - 99
IS  - 4
SP  - 655
EP  - 672
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Pandey, Prashant K.
A1  - Yu, Jing
A1  - Omranian, Nooshin
A1  - Alseekh, Saleh
A1  - Vaid, Neha
A1  - Fernie, Alisdair R.
A1  - Nikoloski, Zoran
A1  - Laitinen, Roosa A. E.
T1  - Plasticity in metabolism underpins local responses to nitrogen in Arabidopsis thaliana populations
JF  - Plant Direct
N2  - Nitrogen (N) is central for plant growth, and metabolic plasticity can provide a strategy to respond to changing N availability. We showed that two local A. thaliana populations exhibited differential plasticity in the compounds of photorespiratory and starch degradation pathways in response to three N conditions. Association of metabolite levels with growth-related and fitness traits indicated that controlled plasticity in these pathways could contribute to local adaptation and play a role in plant evolution.
KW  - Arabidopsis thaliana
KW  - natural variation
KW  - nitrogen availability
KW  - photorespiration
KW  - plasticity
Y1  - 2019
U6  - https://doi.org/10.1002/pld3.186
SN  - 2475-4455
VL  - 3
IS  - 11
PB  - John Wiley & sonst LTD
CY  - Chichester
ER  -