TY  - GEN
A1  - Lou, Ying
A1  - Ma, Hui
A1  - Lin, Wen-Hui
A1  - Chu, Zhao-Quing
A1  - Müller-Röber, Bernd
A1  - Xu, Zhi-Hong
A1  - Xue, Hong-Wei
T1  - The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding
N2  - In Arabidopsis thaliana and Oryza sativa, two types of PI 4-kinase (PI4Ks) have been isolated and functionally characterized. The alpha-type PI4Ks (similar to 220 kDa) contain a PH domain, which is lacking in beta-type PI4Ks (similar to 120 kDa). beta-Type PI4Ks, exemplified by Arabidopsis AtPI4K beta and rice OsPI4K2, contain a highly charged repetitive segment designated PPC (Plant PI4K Charged) region, which is an unique domain only found in plant beta-type PI4Ks at present. The PPC region has a length of similar to 300 amino acids and harboring 11 (AtPI4K beta) and 14 (OsPI4K2) repeats, respectively, of a 20-aa motif. Studies employing a modified yeast-based "Sequence of Membrane- Targeting Detection'' system demonstrate that the PPC(OsPI4K2) region, as well as the former 8 and latter 6 repetitive motifs within the PPC region, are able to target fusion proteins to the plasma membrane. Further detection on the transiently expressed GFP fusion proteins in onion epidermal cells showed that the PPC(OsPI4K2) region alone, as well as the region containing repetitive motifs 1-8, was able to direct GFP to the plasma membrane, while the regions containing less repetitive motifs, i.e. 6, 4, 2 or single motif(s) led to predominantly intracellular localization. Agrobacterium-mediated transient expression of PPC-GFP fusion protein further confirms the membrane-targeting capacities of PPC region. In addition, the predominant plasma membrane localization of AtPI4Kb was mediated by the PPC region. Recombinant PPC peptide, expressed in E. coli, strongly binds phosphatidic acid, PI and PI4P, but not phosphatidylcholine, PI5P, or PI(4,5) P-2 in vitro, providing insights into potential mechanisms for regulating sub- cellular localization and lipid binding for the plant beta-type PI4Ks
Y1  - 2006
UR  - http://www.springerlink.com/content/100330
U6  - https://doi.org/10.1007/s11103-005-5548-x
SN  - 0167-4412
ER  - 
TY  - GEN
A1  - Neye, Gundula
A1  - Wallschläger, Hans-Dieter
A1  - Tiedemann, Ralph
T1  - Song dialect boundaries in the Yellowhammer: Do they restrict gene flow?
T2  - Journal of ornithology
Y1  - 2006
SN  - 0021-8375
VL  - 147
IS  - Supplement 1
SP  - 219
EP  - 219
PB  - Blackwell
CY  - New York
ER  - 
TY  - GEN
A1  - Schmoll, Tim
A1  - Schurr, Frank Martin
A1  - Winkel, W.
A1  - Lubjuhn, Thomas
T1  - Female extra-pair mating, fitness and genetic diversity: Expression in socially monogamous Coal Tits
T2  - Journal of ornithology
Y1  - 2006
SN  - 0021-8375
VL  - 147
SP  - 248
EP  - 248
PB  - Elsevier
CY  - New York
ER  - 
TY  - GEN
A1  - Ten Freyhaus, Henrik
A1  - Huntgeburth, Michael
A1  - Wingler, Kirstin
A1  - Baeumer, A. T.
A1  - Wartenberg, Maria
A1  - Sauer, H.
A1  - Bekhite, Mohamed M.
A1  - Rosenkranz, S.
T1  - Inhibition of ROS liberation by the novel nox inhibitor VAS2870 attenuates PDGF-dependent src activation and chemotaxis, but not proliferation in vascular smooth muscle cells
T2  - European heart journal
Y1  - 2006
SN  - 0195-668X
SN  - 1522-9645
VL  - 27
SP  - 965
EP  - 965
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - GEN
A1  - Tiedemann, Ralph
A1  - Pfautsch, Simone
T1  - MHC evolution in ducks and allies
T2  - Journal of ornithology
Y1  - 2006
SN  - 0021-8375
VL  - 147
IS  - Supplement 1
SP  - 48
EP  - 48
PB  - Blackwell
CY  - New York
ER  -