TY  - JOUR
A1  - Mai, Tobias
A1  - Wolski, Karol
A1  - Puciul-Malinowska, Agnieszka
A1  - Kopyshev, Alexey
A1  - Gräf, Ralph
A1  - Bruns, Michael
A1  - Zapotoczny, Szczepan
A1  - Taubert, Andreas
T1  - Anionic polymer brushes for biomimetic calcium phosphate mineralization
BT  - A surface with application potential in biomaterials
JF  - Polymers
N2  - This article describes the synthesis of anionic polymer brushes and their mineralization with calcium phosphate. The brushes are based on poly(3-sulfopropyl methacrylate potassium salt) providing a highly charged polymer brush surface. Homogeneous brushes with reproducible thicknesses are obtained via surface-initiated atom transfer radical polymerization. Mineralization with doubly concentrated simulated body fluid yields polymer/inorganic hybrid films containing AB-Type carbonated hydroxyapatite (CHAP), a material resembling the inorganic component of bone. Moreover, growth experiments using Dictyostelium discoideum amoebae demonstrate that the mineral-free and the mineral-containing polymer brushes have a good biocompatibility suggesting their use as biocompatible surfaces in implantology or related fields.
KW  - polymer brushes
KW  - calcium phosphate
KW  - hydroxyapatite
KW  - carbonated apatite
KW  - bone mimic
KW  - biocompatibility
KW  - Dictyostelium discoideum
Y1  - 2018
U6  - https://doi.org/10.3390/polym10101165
SN  - 2073-4360
VL  - 10
IS  - 10
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Wang, Weiwei
A1  - Kratz, Karl
A1  - Behl, Marc
A1  - Yan, Wan
A1  - Liu, Yue
A1  - Xu, Xun
A1  - Baudis, Stefan
A1  - Li, Zhengdong
A1  - Kurtz, Andreas
A1  - Lendlein, Andreas
A1  - Ma, Nan
T1  - The interaction of adipose-derived human mesenchymal stem cells and polyether ether ketone
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - Polyether ether ketone (PEEK) as a high-performance, thermoplastic implant material entered the field of medical applications due to its structural function and commercial availability. In bone tissue engineering, the combination of mesenchymal stem cells (MSCs) with PEEK implants may accelerate the bone formation and promote the osseointegration between the implant and the adjacent bone tissue. In this concept the question how PEEK influences the behaviour and functions of MSCs is of great interest. Here the cellular response of human adipose-derived MSCs to PEEK was evaluated and compared to tissue culture plate (TCP) as the reference material. Viability and morphology of cells were not altered when cultured on the PEEK film. The cells on PEEK presented a high proliferation activity in spite of a relatively lower initial cell adhesion rate. There was no significant difference on cell apoptosis and senescence between the cells on PEEK and TCP. The inflammatory cytokines and VEGF secreted by the cells on these two surfaces were at similar levels. The cells on PEEK showed up-regulated BMP2 and down-regulated BMP4 and BMP6 gene expression, whereas no conspicuous differences were observed in the committed osteoblast markers (BGLAP, COL1A1 and Runx2). With osteoinduction the cells on PEEK and TCP exhibited a similar osteogenic differentiation potential. Our results demonstrate the biofunctionality of PEEK for human MSC cultivation and differentiation. Its clinical benefits in bone tissue engineering may be achieved by combining MSCs with PEEK implants. These data may also provide useful information for further modification of PEEK with chemical or physical methods to regulate the cellular processes of MSCs and to consequently improve the efficacy of MSC-PEEK based therapies.
KW  - Polyether ether ketone
KW  - mesenchymal stem cells
KW  - biocompatibility
KW  - cell-material interaction
KW  - osteogenic differentiation
Y1  - 2015
U6  - https://doi.org/10.3233/CH-152001
SN  - 1386-0291
SN  - 1875-8622
VL  - 61
IS  - 2
SP  - 301
EP  - 321
PB  - IOS Press
CY  - Amsterdam
ER  -