TY  - JOUR
A1  - Meyer, Sören
A1  - Lopez-Serrano, Aniceto
A1  - Mitze, Hanna
A1  - Jakubowski, Norbert
A1  - Schwerdtle, Tanja
T1  - Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite
JF  - Metallomics : integrated biometal science
N2  - Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 mu M for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.
Y1  - 2017
U6  - https://doi.org/10.1039/c7mt00285h
SN  - 1756-5901
SN  - 1756-591X
VL  - 10
IS  - 1
SP  - 73
EP  - 76
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -