TY  - JOUR
A1  - Toulouse, Charlotte Marguerite
A1  - Schmucker, Sonja
A1  - Metesch, Kristina
A1  - Pfannstiel, Jens
A1  - Michel, Bernd
A1  - Starke, Ines
A1  - Möller, Heiko Michael
A1  - Stefanski, Volker
A1  - Steuber, Julia
T1  - Mechanism and impact of catecholamine conversion by Vibrio cholerae
JF  - Biochimica et biophysica acta : Bioenergetics
N2  - Bacterial pathogens are influenced by signaling molecules including the catecholamines adrenaline and noradrenaline which are host-derived hormones and neurotransmitters. Adrenaline and noradrenaline modulate growth, motility and virulence of bacteria. We show that adrenaline is converted by the pathogen Vibrio cholerae to adrenochrome in the course of respiration, and demonstrate that superoxide produced by the respiratory, Na+ - translocating NADH:quinone oxidoreductase (NQR) acts as electron acceptor in the oxidative conversion of adrenaline to adrenochrome. Adrenochrome stimulates growth of V. cholerae, and triggers specific responses in V. cholerae and in immune cells. We performed a quantitative proteome analysis of V. cholerae grown in minimal medium with glucose as carbon source without catecholamines, or with adrenaline, noradrenaline or adrenochrome. Significant regulation of proteins participating in iron transport and iron homeostasis, in energy metabolism, and in signaling was observed upon exposure to adrenaline, noradrenaline or adrenochrome. On the host side, adrenochrome inhibited lipopolysaccharide-triggered formation of TNF-alpha by THP-1 monocytes, though to a lesser extent than adrenaline. It is proposed that adrenochrome produced from adrenaline by respiring V. cholerae functions as effector molecule in pathogen-host interaction.
KW  - Vibrio cholerae
KW  - Na+ - NADH:quinone oxidoreductase
KW  - NQR
KW  - Superoxide
KW  - Adrenaline
KW  - Adrenochrome
Y1  - 2019
U6  - https://doi.org/10.1016/j.bbabio.2019.04.003
SN  - 0005-2728
SN  - 1879-2650
VL  - 1860
IS  - 6
SP  - 478
EP  - 487
PB  - Elsevier
CY  - Amsterdam
ER  -