TY  - JOUR
A1  - Czarnocka, Weronika
A1  - Van Der Kelen, Katrien
A1  - Willems, Patrick
A1  - Szechynska-Hebda, Magdalena
A1  - Shahnejat-Bushehri, Sara
A1  - Balazadeh, Salma
A1  - Rusaczonek, Anna
A1  - Müller-Röber, Bernd
A1  - Van Breusegem, Frank
A1  - Karpinski, Stanislaw
T1  - The dual role of LESION SIMULATING DISEASE 1 as a condition-dependent scaffold protein and transcription regulator
JF  - Plant, cell & environment : cell physiology, whole-plant physiology, community physiology
N2  - Since its discovery over two decades ago as an important cell death regulator in Arabidopsis thaliana, the role of LESION SIMULATING DISEASE 1 (LSD1) has been studied intensively within both biotic and abiotic stress responses as well as with respect to plant fitness regulation. However, its molecular mode of action remains enigmatic. Here, we demonstrate that nucleo-cytoplasmic LSD1 interacts with a broad range of other proteins that are engaged in various molecular pathways such as ubiquitination, methylation, cell cycle control, gametogenesis, embryo development and cell wall formation. The interaction of LSD1 with these partners is dependent on redox status, as oxidative stress significantly changes the quantity and types of LSD1-formed complexes. Furthermore, we show that LSD1 regulates the number and size of leaf mesophyll cells and affects plant vegetative growth. Importantly, we also reveal that in addition to its function as a scaffold protein, LSD1 acts as a transcriptional regulator. Taken together, our results demonstrate that LSD1 plays a dual role within the cell by acting as a condition-dependent scaffold protein and as a transcription regulator.
KW  - Arabidopsis
KW  - thaliana
KW  - dry weight
KW  - LSD1
KW  - oxidative stress
KW  - protein interaction
KW  - transcription regulation
Y1  - 2017
U6  - https://doi.org/10.1111/pce.12994
SN  - 0140-7791
SN  - 1365-3040
VL  - 40
SP  - 2644
EP  - 2662
PB  - Wiley
CY  - Hoboken
ER  -