TY  - JOUR
A1  - Zhang, Xiaorong
A1  - Caserta, Giorgio
A1  - Yarman, Aysu
A1  - Supala, Eszter
A1  - Tadjoung Waffo, Armel Franklin
A1  - Wollenberger, Ulla
A1  - Gyurcsanyi, Robert E.
A1  - Zebger, Ingo
A1  - Scheller, Frieder W.
T1  - "Out of Pocket" protein binding
BT  - a dilemma of epitope imprinted polymers revealed for human hemoglobin
JF  - Chemosensors
N2  - The epitope imprinting approach applies exposed peptides as templates to synthesize Molecularly Imprinted Polymers (MIPs) for the recognition of the parent protein. While generally the template protein binding to such MIPs is considered to occur via the epitope-shaped cavities, unspecific interactions of the analyte with non-imprinted polymer as well as the detection method used may add to the complexity and interpretation of the target rebinding. To get new insights on the effects governing the rebinding of analytes, we electrosynthesized two epitope-imprinted polymers using the N-terminal pentapeptide VHLTP-amide of human hemoglobin (HbA) as the template. MIPs were prepared either by single-step electrosynthesis of scopoletin/pentapeptide mixtures or electropolymerization was performed after chemisorption of the cysteine extended VHLTP peptide. Rebinding of the target peptide and the parent HbA protein to the MIP nanofilms was quantified by square wave voltammetry using a redox probe gating, surface enhanced infrared absorption spectroscopy, and atomic force microscopy. While binding of the pentapeptide shows large influence of the amino acid sequence, all three methods revealed strong non-specific binding of HbA to both polyscopoletin-based MIPs with even higher affinities than the target peptides.
KW  - Molecularly Imprinted Polymers
KW  - epitope imprinting
KW  - non-specific
KW  - binding
KW  - redox gating
KW  - SEIRA spectroelectrochemistry
Y1  - 2021
U6  - https://doi.org/10.3390/chemosensors9060128
SN  - 2227-9040
VL  - 9
IS  - 6
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Huang, T.
A1  - Warsinke, Axel
A1  - Koroljova-Skorobogatko, O. V.
A1  - Makower, Alexander
A1  - Kuwana, T.
A1  - Scheller, Frieder W.
T1  - A bienzyme carbon paste electrode for the sensitive detection of NADPH and the measurement of glucose-6- phosphate dehydrogenase
Y1  - 1999
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - A bienzyme electrode for L-malate based on a novel and general design
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Eremenko, A. V.
A1  - Makower, Alexander
A1  - Bauer, Christian G.
A1  - Kurochkin, I. N.
A1  - Scheller, Frieder W.
T1  - A bienzyme electrode for tyrosine containing peptides determination
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Lettau, Kristian
A1  - Warsinke, Axel
A1  - Katterle, Martin
A1  - Danielsson, Bengt
A1  - Scheller, Frieder W.
T1  - A bifunctional molecularly imprinted polymer (MIP): analysis of binding and catalysis by a thermistor
N2  - Binding or catalysis? Both can be distinguished with a molecularly imprinted polymer (MIP) by the different patterns of heat generation. The catalytically active sites, like in the corresponding enzyme, generate a steady-state temperature increase. Thus, enzyme-like catalysis and antibody-analogue binding are analyzed simultaneously in a bifunctional MIP for the first time (see scheme).
Y1  - 2006
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/26737/
U6  - https://doi.org/10.1002/anie.200601796
ER  - 
TY  - JOUR
A1  - Chen, Ziping
A1  - Warsinke, Axel
A1  - Gajovic, Nenad
A1  - Große, St.
A1  - Hu, J.
A1  - Kleber, H.-P.
A1  - Scheller, Frieder W.
T1  - A D-carnitine dehydrogenase electrode for the assessment of enantiomeric purity of L-carnitine preparations
Y1  - 2000
ER  - 
TY  - JOUR
A1  - Song, Min Ik
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - A method to detect superoxide radicals using teflon membrane and superoxide dismutase
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Warsinke, Axel
A1  - Scheller, Frieder W.
T1  - A novel multienzyme electrode for the determination of citrate
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Teller, C.
A1  - Halamek, Jan
A1  - Makower, Alexander
A1  - Fournier, Didier
A1  - Schulze, H.
A1  - Scheller, Frieder W.
T1  - A piezoelectric sensor with propidium as a recognition element for cholinesterases
N2  - A piezoelectric biosensor has been developed on the basis of the reversible acetylcholinesterase (AChE) inhibitor propidium. The propidium cation was bound to a 11-mercaptoundecanoic acid monolayer on gold-coated quartz crystals. The immobilization was done via activation of carboxyl groups by 1,3-dicyclohexylcarbodiimide (DCC). Different types of cholinesterases (acetyl- and butyryl-ChE) from different origins were tested for their binding ability towards the immobilized propidium. Binding Studies were performed in a flow system, Furthermore, catalytically active and organophosphate-inhibited enzyme were compared re-aiding their binding capability. The binding constants were derived by using an one to one binding model and a refined model also including rebinding effects. It was shown that organophosphorylation of the active site hardly influences the affinity of AChE towards propidium. Furthermore the propidium-based biosensor provides equal sensitivity as compared with piezolelectric sensors with immobilized paraoxon- an active site ligand of AChE. (c) 2005 Elsevier B.V. All rights reserved
Y1  - 2006
U6  - https://doi.org/10.1016/j.snb.2005.02.053
ER  - 
TY  - JOUR
A1  - Gajovic, Nenad
A1  - Habermüller, K.
A1  - Warsinke, Axel
A1  - Schuhmann, W.
A1  - Scheller, Frieder W.
T1  - A pyruvate oxidase electrode based on an electrochemically deposited redox polymer
Y1  - 1999
ER  -