TY  - JOUR
A1  - Tentschert, J.
A1  - Draude, F.
A1  - Jungnickel, H.
A1  - Haase, A.
A1  - Mantion, Alexandre
A1  - Galla, S.
A1  - Thuenemann, Andreas F.
A1  - Taubert, Andreas
A1  - Luch, A.
A1  - Arlinghaus, H. F.
T1  - TOF-SIMS analysis of cell membrane changes in functional impaired human macrophages upon nanosilver treatment
JF  - Surface and interface analysis : an international journal devoted to the development and application of techniques for the analysis surfaces, interfaces and thin films
N2  - Silver nanoparticles (SNP) are among the most commercialized nanoparticles. Here, we show that peptide-coated SNP cause functional impairment of human macrophages. A dose-dependent inhibition of phagocytosis is observed after nanoparticle treatment, and pretreatment of cells with N-acetyl cysteine (NAC) can counteract the phagocytosis disturbances caused by SNP.
 Using the surface-sensitive mode of time-of-flight secondary ion mass spectrometry, in combination with multivariate statistical methods, we studied the composition of cell membranes in human macrophages upon exposure to SNP with and without NAC preconditioning. This method revealed characteristic changes in the lipid pattern of the cellular membrane outer leaflet in those cells challenged by SNP. Statistical analyses resulted in 19 characteristic ions, which can be used to distinguish between NAC pretreated and untreated macrophages. The present study discusses the assignments of surface cell membrane phospholipids for the identified ions and the resulting changes in the phospholipid pattern of treated cells. We conclude that the adverse effects in human macrophages caused by SNP can be partially reversed through NAC administration. Some alterations, however, remained.
KW  - silver nanoparticles
KW  - lipidomics
KW  - N-acetyl cysteine
KW  - phagocytosis
KW  - oxidative stress
Y1  - 2013
U6  - https://doi.org/10.1002/sia.5155
SN  - 0142-2421
VL  - 45
IS  - 1
SP  - 483
EP  - 485
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Jüppner, Jessica
A1  - Mubeen, Umarah
A1  - Leisse, Andrea
A1  - Caldana, Camila
A1  - Brust, Henrike
A1  - Steup, Martin
A1  - Herrmann, Marion
A1  - Steinhauser, Dirk
A1  - Giavalisco, Patrick
T1  - Dynamics of lipids and metabolites during the cell cycle of Chlamydomonas reinhardtii
JF  - The plant journal
N2  - Metabolites and lipids are the final products of enzymatic processes, distinguishing the different cellular functions and activities of single cells or whole tissues. Understanding these cellular functions within a well-established model system requires a systemic collection of molecular and physiological information. In the current report, the green alga Chlamydomonas reinhardtii was selected to establish a comprehensive workflow for the detailed multi-omics analysis of a synchronously growing cell culture system. After implementation and benchmarking of the synchronous cell culture, a two-phase extraction method was adopted for the analysis of proteins, lipids, metabolites and starch from a single sample aliquot of as little as 10-15million Chlamydomonas cells. In a proof of concept study, primary metabolites and lipids were sampled throughout the diurnal cell cycle. The results of these time-resolved measurements showed that single compounds were not only coordinated with each other in different pathways, but that these complex metabolic signatures have the potential to be used as biomarkers of various cellular processes. Taken together, the developed workflow, including the synchronized growth of the photoautotrophic cell culture, in combination with comprehensive extraction methods and detailed metabolic phenotyping has the potential for use in in-depth analysis of complex cellular processes, providing essential information for the understanding of complex biological systems.
KW  - Chlamydomonas reinhardtii
KW  - synchronized cell cultures
KW  - photoautotrophic growth
KW  - cell cycle
KW  - metabolomics
KW  - lipidomics
KW  - systems biology
KW  - two-phase extraction
KW  - diurnal cycle
KW  - technical advance
Y1  - 2017
U6  - https://doi.org/10.1111/tpj.13642
SN  - 0960-7412
SN  - 1365-313X
VL  - 92
SP  - 331
EP  - 343
PB  - Wiley
CY  - Hoboken
ER  -