TY  - JOUR
A1  - Pinyou, Piyanut
A1  - Ruff, Adrian
A1  - Poeller, Sascha
A1  - Alsaoub, Sabine
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Schuhmann, Wolfgang
T1  - Wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces via entrapment in low potential phenothiazine-modified redox polymers
JF  - Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society
N2  - Phenothiazine-modified redox hydrogels were synthesized and used for the wiring of the aldehyde oxidoreductase PaoABC to electrode surfaces. The effects of the pH value and electrode surface modification on the biocatalytic activity of the layers were studied in the presence of vanillin as the substrate. The enzyme electrodes were successfully employed as bioanodes in vanillin/O-2 biofuel cells in combination with a high potential bilirubin oxidase biocathode. Open circuit voltages of around 700 mV could be obtained in a two compartment biofuel cell setup. Moreover, the use of a rather hydrophobic polymer with a high degree of crosslinking sites ensures the formation of stable polymer/enzyme films which were successfully used as bioanode in membrane-less biofuel cells. (C) 2015 Elsevier B.V. All rights reserved.
KW  - Aldehyde oxidoreductase
KW  - Enzyme electrode
KW  - Redox polymer
KW  - Phenothiazine
KW  - Biosensor
KW  - Biofuel cell
Y1  - 2016
U6  - https://doi.org/10.1016/j.bioelechem.2015.12.005
SN  - 1567-5394
SN  - 1878-562X
VL  - 109
SP  - 24
EP  - 30
PB  - Elsevier
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Contin, Andrea
A1  - Frasca, Stefano
A1  - Vivekananthan, Jeevanthi
A1  - Leimkühler, Silke
A1  - Wollenberger, Ursula
A1  - Plumere, Nicolas
A1  - Schuhmann, Wolfgang
T1  - A pH Responsive Redox Hydrogel for Electrochemical Detection of Redox Silent Biocatalytic Processes. Control of Hydrogel Solvation
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - The control of bioelectrocatalytic processes by external stimuli for the indirect detection of non-redox active species was achieved using an esterase and a redox enzyme both integrated within a redox hydrogel. The poly( vinyl) imidazole Os(bpy)(2)Cl hydrogel displays pH-responsive properties. The esterase catalysed reaction leads to a local pH decrease causing protonation of imidazole moieties thus increasing hydrogel solvation and mobility of the tethered Os-complexes. This is the key step to enable improved electron transfer between an aldehyde oxidoreductase and the polymer-bound Os-complexes. The off-on switch is further integrated in a biofuel cell system for self-powered signal generation.
KW  - pH responsive hydrogel
KW  - External stimuli
KW  - Biofuel cell
KW  - Self-powered biosensor
KW  - Solvation
Y1  - 2015
U6  - https://doi.org/10.1002/elan.201400621
SN  - 1040-0397
SN  - 1521-4109
VL  - 27
IS  - 4
SP  - 938
EP  - 944
PB  - Wiley-VCH
CY  - Weinheim
ER  -