TY  - JOUR
A1  - Schenk, Jörg A.
A1  - Fettke, Jörg
A1  - Lenz, Christine
A1  - Albers, Katharina
A1  - Mallwitz, Frank
A1  - Gajovic-Eichelmann, Nenad
A1  - Ehrentreich-Förster, Eva
A1  - Kusch, Emely
A1  - Sellrie, Frank
T1  - Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G
JF  - Journal of biotechnology
N2  - The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.
KW  - Hybridoma
KW  - SLPI
KW  - Protein G
KW  - Progesterone
KW  - Serum-free
Y1  - 2012
U6  - https://doi.org/10.1016/j.jbiotec.2011.12.025
SN  - 0168-1656
VL  - 158
IS  - 1-2
SP  - 34
EP  - 35
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Ettlinger, Julia
A1  - Schenk, Jörg A.
A1  - Micheel, Burkhard
A1  - Ehrentreich-Förster, Eva
A1  - Gajovic-Eichelmann, Nenad
T1  - A direct competitive homogeneous immunoassay for progesterone - the Redox Quenching Immunoassay
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - A direct competitive amperometric immunoassay format for the detection of haptens and proteins was developed. The method is based on the quenching of electroactivity of ferrocenium, which is coupled to the antigen and used as the primary reporter, upon binding to a monoclonal anti-ferrocenium antibody, which is coupled to the detection antibody and used as a secondary reporter. A separation-free progesterone immunoassay with a lower detection limit of 1 ng?mL-1 (3.18 nmol?L-1) in 1?:?2 diluted blood serum was realised by combining two bifunctional conjugates, a ferrocenium-PEG-progesterone tracer and a bioconjugate of one anti-progesterone and one anti-ferrocenium antibody. The immune complex is formed within 30 s upon addition of progesterone, resulting in a total analysis time of 1.5 min.
KW  - Immunoassay
KW  - Amperometry
KW  - Ferrocene
KW  - Progesterone
Y1  - 2012
U6  - https://doi.org/10.1002/elan.201200107
SN  - 1040-0397
VL  - 24
IS  - 7
SP  - 1567
EP  - 1575
PB  - Wiley-VCH
CY  - Weinheim
ER  -