TY  - JOUR
A1  - Lu, Yong-Ping
A1  - Reichetzeder, Christoph
A1  - Prehn, Cornelia
A1  - von Websky, Karoline
A1  - Slowinski, Torsten
A1  - Chen, You-Peng
A1  - Yin, Liang-Hong
A1  - Kleuser, Burkhard
A1  - Yang, Xue-Song
A1  - Adamski, Jerzy
A1  - Hocher, Berthold
T1  - Fetal serum metabolites are independently associated with Gestational diabetes mellitus
JF  - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology
N2  - Background/Aims: Gestational diabetes (GDM) might be associated with alterations in the metabolomic profile of affected mothers and their offspring. Until now, there is a paucity of studies that investigated both, the maternal and the fetal serum metabolome in the setting of GDM. Mounting evidence suggests that the fetus is not just passively affected by gestational disease but might play an active role in it. Metabolomic studies performed in maternal blood and fetal cord blood could help to better discern distinct fetal from maternal disease interactions. Methods: At the time of birth, serum samples from mothers and newborns (cord blood samples) were collected and screened for 163 metabolites utilizing tandem mass spectrometry. The cohort consisted of 412 mother/child pairs, including 31 cases of maternal GDM. Results: An initial non-adjusted analysis showed that eight metabolites in the maternal blood and 54 metabolites in the cord blood were associated with GDM. After Benjamini-Hochberg (BH) procedure and adjustment for confounding factors for GDM, fetal phosphatidylcholine acyl-alkyl C 32:1 and proline still showed an independent association with GDM. Conclusions: This study found metabolites in cord blood which were associated with GDM, even after adjustment for established risk factors of GDM. To the best of our knowledge, this is the first study demonstrating an independent association between fetal serum metabolites and maternal GDM. Our findings might suggest a potential effect of the fetal metabolome on maternal GDM. (c) 2018 The Author(s) Published by S. Karger AG, Basel
KW  - Gestational diabetes
KW  - Metabolomics
KW  - Phosphatidylcholine acyl-alkyl C 32:1
KW  - Proline
Y1  - 2018
U6  - https://doi.org/10.1159/000487119
SN  - 1015-8987
SN  - 1421-9778
VL  - 45
IS  - 2
SP  - 625
EP  - 638
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Lu, Yong-Ping
A1  - Reichetzeder, Christoph
A1  - Prehn, Cornelia
A1  - Yin, Liang-Hong
A1  - Yun, Chen
A1  - Zeng, Shufei
A1  - Chu, Chang
A1  - Adamski, Jerzy
A1  - Hocher, Berthold
T1  - Cord blood Lysophosphatidylcholine 16:1 is positively associated with birth weight
JF  - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology
N2  - Background/Aims: Impaired birth outcomes, like low birth weight, have consistently been associated with increased disease susceptibility to hypertension in later life. Alterations in the maternal or fetal metabolism might impact on fetal growth and influence birth outcomes. Discerning associations between the maternal and fetal metabolome and surrogate parameters of fetal growth could give new insight into the complex relationship between intrauterine conditions, birth outcomes, and later life disease susceptibility. Methods: Using flow injection tandem mass spectrometry, targeted metabolomics was performed in serum samples obtained from 226 mother/child pairs at delivery. Associations between neonatal birth weight and concentrations of 163 maternal and fetal metabolites were analyzed. Results: After FDR adjustment using the Benjamini-Hochberg procedure lysophosphatidylcholines (LPC) 14:0, 16:1, and 18:1 were strongly positively correlated with birth weight. In a stepwise linear regression model corrected for established confounding factors of birth weight, LPC 16: 1 showed the strongest independent association with birth weight (CI: 93.63 - 168.94; P = 6.94x10(-11)). The association with birth weight was stronger than classical confounding factors such as offspring sex (CI: - 258.81- -61.32; P = 0.002) and maternal smoking during pregnancy (CI: -298.74 - -29.51; P = 0.017). Conclusions: After correction for multiple testing and adjustment for potential confounders, LPC 16:1 showed a very strong and independent association with birth weight. The underlying molecular mechanisms linking fetal LPCs with birth weight need to be addressed in future studies. (c) 2018 The Author(s) Published by S. Karger AG, Basel
KW  - Metabolomics
KW  - Lysophosphatidylcholine
KW  - Birth Weight
KW  - DOHaD
KW  - Hypertension
KW  - Type 2 Diabetes
Y1  - 2018
U6  - https://doi.org/10.1159/000487118
SN  - 1015-8987
SN  - 1421-9778
VL  - 45
IS  - 2
SP  - 614
EP  - 624
PB  - Karger
CY  - Basel
ER  - 
TY  - JOUR
A1  - Muiva-Mutisya, Lois M.
A1  - Atilaw, Yoseph
A1  - Heydenreich, Matthias
A1  - Koch, Andreas
A1  - Akala, Hoseah M.
A1  - Cheruiyot, Agnes C.
A1  - Brown, Matthew L.
A1  - Irungu, Beatrice
A1  - Okalebo, Faith A.
A1  - Derese, Solomon
A1  - Mutai, Charles
A1  - Yenesew, Abiy
T1  - Antiplasmodial prenylated flavanonols from Tephrosia subtriflora
JF  - Natural Product Research
N2  - The CH2Cl2/MeOH (1:1) extract of the aerial parts of Tephrosia subtriflora afforded a new flavanonol, named subtriflavanonol (1), along with the known flavanone spinoflavanone B, and the known flavanonols MS-II (2) and mundulinol. The structures were elucidated by the use of NMR spectroscopy and mass spectrometry. The absolute configuration of the flavanonols was determined based on quantum chemical ECD calculations. In the antiplasmodial assay, compound 2 showed the highest activity against chloroquine-sensitive Plasmodiumfalciparum reference clones (D6 and 3D7), artemisinin-sensitive isolate (F32-TEM) as well as field isolate (KSM 009) with IC50 values 1.4-4.6M without significant cytotoxicity against Vero and HEp2 cell lines (IC50>100M). The new compound (1) showed weak antiplasmodial activity, IC50 12.5-24.2M, but also showed selective anticancer activity against HEp2 cell line (CC50 16.9M). [GRAPHICS] .
KW  - Tephrosia subtriflora
KW  - Leguminosae
KW  - prenylated flavanonol
KW  - subtriflavanonol
KW  - antiplasmodial
KW  - cytotoxicity
Y1  - 2018
U6  - https://doi.org/10.1080/14786419.2017.1353510
SN  - 1478-6419
SN  - 1478-6427
VL  - 32
IS  - 12
SP  - 1407
EP  - 1414
PB  - Routledge, Taylor & Francis Group
CY  - Abingdon
ER  - 
TY  - JOUR
A1  - Krüger-Genge, Anne
A1  - Schulz, Christian
A1  - Kratz, Karl
A1  - Lendlein, Andreas
A1  - Jung, Friedrich
T1  - Comparison of two substrate materials used as negative control in endothelialization studies
BT  - Glass versus polymeric tissue culture plate
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - The endothelialization of synthetic surfaces applied as cardiovascular implant materials is an important issue to ensure the anti-thrombotic quality of a biomaterial. However, the rapid and constant development of a functionallycon-fluent endothelial cell monolayer is challenging. In order to investigate the compatibility of potential implant materials with endothelial cells several in vitro studies are performed. Here, glass and tissue culture plates (TCP) are often used as reference materials for in vitro pre-testing. However, a direct comparison of both substrates is lacking. Therefore, a comparison of study results is difficult, since results are often related to various reference materials. In this study, the endothelialization of glass and TCP was investigated in terms of adherence, morphology, integrity, viability and function using human umbilical vein endothelial cells (HUVEC). On both substrates an almost functionally confluent HUVEC monolayer was developed after nine days of cell seeding with clearly visible cell rims, decreased stress fiber formation and a pronounced marginal filament band. The viability of HUVEC was comparable for both substrates nine days after cell seeding with only a few dead cells. According to that, the cell membrane integrity as well as the metabolic activity showed no differences between TCP and glass. However, a significant difference was observed for the secretion of IL-6 and IL-8. The concentration of both cytokines, which are associated with migratory activity, was increased in the supernatant of HUVEC seeded on TCP. This result matches well with the slightly increased number of adherent HUVEC on TCP. In conclusion, these findings indicate that both reference materials are almost comparable and can be used equivalently as control materials in in vitro endothelialization studies.
KW  - Negative control
KW  - endothelial cells
KW  - glass
KW  - TCP
KW  - reference
Y1  - 2018
U6  - https://doi.org/10.3233/CH-189904
SN  - 1386-0291
SN  - 1875-8622
VL  - 69
IS  - 3
SP  - 437
EP  - 445
PB  - IOS Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Krüger-Genge, A.
A1  - Braune, S.
A1  - Walter, M.
A1  - Krengel, M.
A1  - Kratz, K.
A1  - Küpper, J. H.
A1  - Lendlein, Andreas
A1  - Jung, Friedrich
T1  - Influence of different surface treatments of poly(n-butyl acrylate) networks on fibroblasts adhesion, morphology and viability
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - BACKGROUND: Physical and chemical characteristics of implant materials determine the fate of long-term cardiovascular devices. However, there is still a lack of fundamental understanding of the molecular mechanisms occurring in the material-tissue interphase. In a previous study, soft covalently crosslinked poly(n-butyl acrylate) networks (cPnBA) were introduced as sterilizable, non-toxic and immuno-compatible biomaterials with mechanical properties adjustable to blood vessels. Here we study the influence of different surface treatments in particular oxygen plasma modification and fibrinogen deposition as well as a combinatorial approach on the adhesion and viability of fibroblasts. RESULTS: Compared to non-treated cPnBAs the advancing water-contact angles were found to be reduced after all surface modifications (p<0.05, each), while lowest values were observed after the combined surface treatment (OPT+FIB). The latter differed significantly from the single OPT and FIB. The number of adherent fibroblasts and their adherence behavior differed on both pristine cPnBA networks. The fibroblast density on cPnBA04 was 743 +/- 434 cells. mm(-2), was about 6.5 times higher than on cPnBA73 with 115 +/- 73 cells. mm(-2). On cPnBA04 about 20% of the cells were visible as very small, round and buckled cells while all other cells were in a migrating status. On cPnBA73, nearly 50% of fibroblasts were visible as very small, round and buckled cells. The surface functionalization either using oxygen plasma treatment or fibrinogen coating led to a significant increase of adherent fibroblasts, particularly the combination of both techniques, for both cPnBA networks. It is noteworthy to mention that the fibrinogen coating overruled the characteristics of the pristine surfaces; here, the fibroblast densities after seeding were identical for both cPnBAnetworks. Thus, the binding rather depended on the fibrinogen coating than on the substrate characteristics anymore. While the integrity of the fibroblasts membrane was comparable for both polymers, the MTS tests showed a decreased metabolic activity of the fibroblasts on cPnBA. CONCLUSION: The applied surface treatments of cPnBA successfully improved the adhesion of viable fibroblasts. Under resting conditions as well as after shearing the highest fibroblast densities were found on surfaces with combined post-treatment.
KW  - Biomaterial
KW  - poly(n-butyl acrylate)
KW  - fibroblast
KW  - oxygen plasma
KW  - fibrinogen
KW  - cell adhesion
KW  - focal adhesion
KW  - actin cytoskeleton
KW  - viability
Y1  - 2018
U6  - https://doi.org/10.3233/CH-189130
SN  - 1386-0291
SN  - 1875-8622
VL  - 69
IS  - 1-2
SP  - 305
EP  - 316
PB  - IOS Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Kumar, Reddi K.
A1  - Heuchel, Matthias
A1  - Kratz, Karl
A1  - Lendlein, Andreas
A1  - Jankowski, Joachim
A1  - Tetali, Sarada D.
T1  - Effects of extracts prepared from modified porous poly(ether imide) microparticulate absorbers on cytotoxicity, macrophage differentiation and proinflammatory behavior of human monocytic (THP-1) cells
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - Remaining uremic toxins in the blood of chronic renal failure patients represent one central challenge in hemodialysis therapies. Highly porous poly(ether imide) (PEI) microparticles have been recently introduced as candidate absorber materials, which show a high absorption capacity for uremic toxins and allow hydrophilic surface modification suitable for minimization of serum protein absorption. In this work, the effects of extracts prepared from PEI microparticles modified by nucleophilic reaction with low molecular weight polyethylene imine (Pei) or potassium hydroxide (KOH), on human monocytic (THP-1) cells are studied. The obtained results suggested that the extracts of Pei and KOH modified PEI absorbers have no negative effect on THP-1 cell viability and do not initiate the critical differentiation towards macrophages. The extracts did not enhance transcript or protein levels of investigated proinflammatory markers in THP-1 cells, namely, TNF alpha, MCP1, IL6 and IL8. Based on these findings such modified PEI microparticles should be qualified for further pre-clinical evaluation i.e. in an in vivo animal experiment.
KW  - Chronic kidney disease
KW  - hemodialysis
KW  - Inflammation
KW  - Porous poly(ether imide) microparticulate absorbers
KW  - THP-1 cells
KW  - Uremic toxins
Y1  - 2018
U6  - https://doi.org/10.3233/CH-189112
SN  - 1386-0291
SN  - 1875-8622
VL  - 69
IS  - 1-2
SP  - 175
EP  - 185
PB  - IOS Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Giulbudagian, Michael
A1  - Yealland, Guy
A1  - Hönzke, S.
A1  - Edlich, A.
A1  - Geisendörfer, Birte
A1  - Kleuser, Burkhard
A1  - Hedtrich, Sarah
A1  - Calderon, Marcelo
T1  - Breaking the Barrier
BT  - potent anti-inflammatory activity following efficient topical delivery of etanercept using thermoresponsive nanogels
JF  - Theranostics
N2  - Topical administration permits targeted, sustained delivery of therapeutics to human skin. Delivery to the skin, however, is typically limited to lipophilic molecules with molecular weight of < 500 Da, capable of crossing the stratum corneum. Nevertheless, there are indications protein delivery may be possible in barrier deficient skin, a condition found in several inflammatory skin diseases such as psoriasis, using novel nanocarrier systems. Methods: Water in water thermo-nanoprecipitation; dynamic light scattering; zeta potential measurement; nanoparticle tracking analysis; atomic force microscopy; cryogenic transmission electron microscopy; UV absorption; centrifugal separation membranes; bicinchoninic acid assay; circular dichroism; TNF alpha binding ELISA; inflammatory skin equivalent construction; human skin biopsies; immunohistochemistry; fluorescence microscopy; western blot; monocyte derived Langerhans cells; ELISA Results: Here, we report the novel synthesis of thermoresponsive nanogels (tNG) and the stable encapsulation of the anti-TNFa fusion protein etanercept (ETR) (similar to 150 kDa) without alteration to its structure, as well as temperature triggered release from the tNGs. Novel tNG synthesis without the use of organic solvents was conducted, permitting in situ encapsulation of protein during assembly, something that holds great promise for easy manufacture and storage. Topical application of ETR loaded tNGs to inflammatory skin equivalents or tape striped human skin resulted in efficient ETR delivery throughout the SC and into the viable epidermis that correlated with clear anti-inflammatory effects. Notably, effective ETR delivery depended on temperature triggered release following topical application. Conclusion: Together these results indicate tNGs hold promise as a biocompatible and easy to manufacture vehicle for stable protein encapsulation and topical delivery into barrier-deficient skin.
KW  - thermoresponsive-nanogel
KW  - topical
KW  - anti-inflammatory therapy
KW  - etanercept
KW  - skin equivalents
Y1  - 2018
U6  - https://doi.org/10.7150/thno.21668
SN  - 1838-7640
VL  - 8
IS  - 2
SP  - 450
EP  - 463
PB  - Ivyspring International Publisher
CY  - Lake haven
ER  - 
TY  - JOUR
A1  - Zimmermann, Marc
A1  - Grigoriev, Dmitry
A1  - Puretskiy, Nikolay
A1  - Böker, Alexander
T1  - Characteristics of microcontact printing with polyelectrolyte ink for the precise preparation of patches on silica particles
JF  - RSC Advances
N2  - This publication demonstrates the abilities of a precise and straightforward microcontact printing approach for the preparation of patchy silica particles. In a broad particle size range, it is possible to finely tune the number and parameters of three-dimensional patches like diameter and thickness using only polyethyleneimine ink, poly(dimethoxysilane) as stamp material and a suitable release solvent.
Y1  - 2018
U6  - https://doi.org/10.1039/c8ra07955b
SN  - 2046-2069
VL  - 8
IS  - 69
SP  - 39241
EP  - 39247
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Ellis, S. C.
A1  - Bauer, S.
A1  - Bacigalupo, C.
A1  - Bland-Hawthorn, J.
A1  - Bryant, J. J.
A1  - Case, S.
A1  - Content, R.
A1  - Fechner, T.
A1  - Giannone, D.
A1  - Haynes, R.
A1  - Hernandez, E.
A1  - Horton, A. J.
A1  - Klauser, U.
A1  - Lawrence, J. S.
A1  - Leon-Saval, S. G.
A1  - Lindley, E.
A1  - Löhmannsröben, Hans-Gerd
A1  - Min, S. -S.
A1  - Pai, N.
A1  - Roth, M.
A1  - Shortridge, K.
A1  - Waller, L.
A1  - Xavier, Pascal
A1  - Zhelem, Ross
T1  - PRAXIS: an OH suppression optimised near infrared spectrograph
T2  - Ground-based and Airborne Instrumentation for Astronomy VII
N2  - The problem of atmospheric emission from OH molecules is a long standing problem for near-infrared astronomy. PRAXIS is a unique spectrograph which is fed by fibres that remove the OH background and is optimised specifically to benefit from OH-Suppression. The OH suppression is achieved with fibre Bragg gratings, which were tested successfully on the GNOSIS instrument. PRAXIS uses the same fibre Bragg gratings as GNOSIS in its first implementation, and will exploit new, cheaper and more efficient, multicore fibre Bragg gratings in the second implementation. The OH lines are suppressed by a factor of similar to 1000, and the expected increase in the signal-to-noise in the interline regions compared to GNOSIS is a factor of similar to 9 with the GNOSIS gratings and a factor of similar to 17 with the new gratings. PRAXIS will enable the full exploitation of OH suppression for the first time, which was not achieved by GNOSIS (a retrofit to an existing instrument that was not OH-Suppression optimised) due to high thermal emission, low spectrograph transmission and detector noise. PRAXIS has extremely low thermal emission, through the cooling of all significantly emitting parts, including the fore-optics, the fibre Bragg gratings, a long length of fibre, and the fibre slit, and an optical design that minimises leaks of thermal emission from outside the spectrograph. PRAXIS has low detector noise through the use of a Hawaii-2RG detector, and a high throughput through a efficient VPH based spectrograph. PRAXIS will determine the absolute level of the interline continuum and enable observations of individual objects via an IFU. In this paper we give a status update and report on acceptance tests.
KW  - Near infrared
KW  - spectroscopy
KW  - OH suppression
KW  - astrophotonics
KW  - fibre Bragg gratings
Y1  - 2018
SN  - 978-1-5106-1958-6
U6  - https://doi.org/10.1117/12.2311898
SN  - 0277-786X
SN  - 1996-756X
VL  - 10702
PB  - SPIE-INT Soc Optical Engineering
CY  - Bellingham
ER  - 
TY  - JOUR
A1  - Yarman, Aysu
A1  - Kurbanoglu, Sevinc
A1  - Jetzschmann, Katharina J.
A1  - Ozkan, Sibel A.
A1  - Wollenberger, Ulla
A1  - Scheller, Frieder W.
T1  - Electrochemical MIP-Sensors for Drugs
JF  - Current Medicinal Chemistry
N2  - In order to replace bio-macromolecules by stable synthetic materials in separation techniques and bioanalysis biomimetic receptors and catalysts have been developed: Functional monomers are polymerized together with the target analyte and after template removal cavities are formed in the "molecularly imprinted polymer" (MIP) which resemble the active sites of antibodies and enzymes. Starting almost 80 years ago, around 1,100 papers on MIPs were published in 2016. Electropolymerization allows to deposit MIPs directly on voltammetric electrodes or chips for quartz crystal microbalance (QCM) and surface plasmon resonance (SPR). For the readout of MIPs for drugs amperometry, differential pulse voltammetry (DPV) and impedance spectroscopy (EIS) offer higher sensitivity as compared with QCM or SPR. Application of simple electrochemical devices allows both the reproducible preparation of MIP sensors, but also the sensitive signal generation. Electrochemical MIP-sensors for the whole arsenal of drugs, e.g. the most frequently used analgesics, antibiotics and anticancer drugs have been presented in literature and tested under laboratory conditions. These biomimetic sensors typically have measuring ranges covering the lower nano-up to millimolar concentration range and they are stable under extreme pH and in organic solvents like nonaqueous extracts.
KW  - Biomimetic sensors
KW  - molecularly imprinted polymers
KW  - drug sensors
KW  - drug imprinting
KW  - electropolymerization
KW  - electrochemical sensors
Y1  - 2018
U6  - https://doi.org/10.2174/0929867324666171005103712
SN  - 0929-8673
SN  - 1875-533X
VL  - 25
IS  - 33
SP  - 4007
EP  - 4019
PB  - Bentham Science Publishers LTD
CY  - Sharjah
ER  -