TY  - JOUR
A1  - Brechun, Katherine Emily
A1  - Arndt, Katja Maren
A1  - Woolley, G. Andrew
T1  - Selection of protein-protein interactions of desired affinities with a bandpass circuit
JF  - Journal of molecular biology : JMB
N2  - We have developed a genetic circuit in Escherichia coli that can be used to select for protein-protein interactions of different strengths by changing antibiotic concentrations in the media. The genetic circuit links protein-protein interaction strength to beta-lactamase activity while simultaneously imposing tuneable positive and negative selection pressure for beta-lactamase activity. Cells only survive if they express interacting proteins with affinities that fall within set high- and low-pass thresholds; i.e. the circuit therefore acts as a bandpass filter for protein-protein interactions. We show that the circuit can be used to recover protein-protein interactions of desired affinity from a mixed population with a range of affinities. The circuit can also be used to select for inhibitors of protein-protein interactions of defined strength. (C) 2018 Elsevier Ltd. All rights reserved.
KW  - synthetic biology
KW  - genetic circuit
KW  - biological engineering
KW  - protein-protein interactions
KW  - twin-arginine translocation
KW  - selection system
Y1  - 2018
U6  - https://doi.org/10.1016/j.jmb.2018.11.011
SN  - 0022-2836
SN  - 1089-8638
VL  - 431
IS  - 2
SP  - 391
EP  - 400
PB  - Elsevier
CY  - London
ER  -