TY  - JOUR
A1  - Pitzen, Valentin
A1  - Askarzada, Sophie
A1  - Gräf, Ralph
A1  - Meyer, Irene
T1  - CDK5RAP2 Is an Essential Scaffolding Protein of the Corona of the Dictyostelium Centrosome
JF  - Cells
N2  - Dictyostelium centrosomes consist of a nucleus-associated cylindrical, three-layered core structure surrounded by a corona consisting of microtubule-nucleation complexes embedded in a scaffold of large coiled-coil proteins. One of them is the conserved CDK5RAP2 protein. Here we focus on the role of Dictyostelium CDK5RAP2 for maintenance of centrosome integrity, its interaction partners and its dynamic behavior during interphase and mitosis. GFP-CDK5RAP2 is present at the centrosome during the entire cell cycle except from a short period during prophase, correlating with the normal dissociation of the corona at this stage. RNAi depletion of CDK5RAP2 results in complete disorganization of centrosomes and microtubules suggesting that CDK5RAP2 is required for organization of the corona and its association to the core structure. This is in line with the observation that overexpressed GFP-CDK5RAP2 elicited supernumerary cytosolic MTOCs. The phenotype of CDK5RAP2 depletion was very reminiscent of that observed upon depletion of CP148, another scaffolding protein of the corona. BioID interaction assays revealed an interaction of CDK5RAP2 not only with the corona markers CP148, gamma-tubulin, and CP248, but also with the core components Cep192, CP75, and CP91. Furthermore, protein localization studies in both depletion strains revealed that CP148 and CDK5RAP2 cooperate in corona organization.
KW  - centrosome
KW  - centriole
KW  - Dictyostelium
KW  - microtubules
KW  - mitosis
Y1  - 2018
U6  - https://doi.org/10.3390/cells7040032
SN  - 2073-4409
VL  - 7
IS  - 4
PB  - MDPI
CY  - Basel
ER  - 
TY  - GEN
A1  - Gräf, Ralph
T1  - Comparative Biology of Centrosomal Structures in Eukaryotes
T2  - Cells
N2  - The centrosome is not only the largest and most sophisticated protein complex within a eukaryotic cell, in the light of evolution, it is also one of its most ancient organelles. This special issue of "Cells" features representatives of three main, structurally divergent centrosome types, i.e., centriole-containing centrosomes, yeast spindle pole bodies (SPBs), and amoebozoan nucleus-associated bodies (NABs). Here, I discuss their evolution and their key-functions in microtubule organization, mitosis, and cytokinesis. Furthermore, I provide a brief history of centrosome research and highlight recently emerged topics, such as the role of centrioles in ciliogenesis, the relationship of centrosomes and centriolar satellites, the integration of centrosomal structures into the nuclear envelope and the involvement of centrosomal components in non-centrosomal microtubule organization.
KW  - centrosome
KW  - centriole
KW  - cilium
KW  - basal body
KW  - spindle pole body
KW  - SPB
KW  - nucleus-associated body
KW  - NAB
KW  - microtubules
Y1  - 2018
U6  - https://doi.org/10.3390/cells7110202
SN  - 2073-4409
VL  - 7
IS  - 11
PB  - MDPI
CY  - Basel
ER  - 
TY  - GEN
A1  - Gräf, Ralph
T1  - Comparative biology of centrosomal structures in eukaryotes
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The centrosome is not only the largest and most sophisticated protein complex within a eukaryotic cell, in the light of evolution, it is also one of its most ancient organelles. This special issue of "Cells" features representatives of three main, structurally divergent centrosome types, i.e., centriole-containing centrosomes, yeast spindle pole bodies (SPBs), and amoebozoan nucleus-associated bodies (NABs). Here, I discuss their evolution and their key-functions in microtubule organization, mitosis, and cytokinesis. Furthermore, I provide a brief history of centrosome research and highlight recently emerged topics, such as the role of centrioles in ciliogenesis, the relationship of centrosomes and centriolar satellites, the integration of centrosomal structures into the nuclear envelope and the involvement of centrosomal components in non-centrosomal microtubule organization.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1063 
KW  - centrosome
KW  - centriole
KW  - cilium
KW  - basal body
KW  - spindle pole body
KW  - SPB
KW  - nucleus-associated body
KW  - NAB
KW  - microtubules
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-472294
SN  - 1866-8372
IS  - 1063
ER  -