TY - GEN
A1 - Zemella, Anne
A1 - Thoring, Lena
A1 - Hoffmeister, Christian
A1 - Šamalíková, Mária
A1 - Ehren, Patricia
A1 - Wüstenhagen, Doreen Anja
A1 - Kubick, Stefan
T1 - Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin
T2 - Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe
N2 - As one of the most complex post-translational modification, glycosylation is widely involved in cell adhesion, cell proliferation and immune response. Nevertheless glycoproteins with an identical polypeptide backbone mostly differ in their glycosylation patterns. Due to this heterogeneity, the mapping of different glycosylation patterns to their associated function is nearly impossible. In the last years, glycoengineering tools including cell line engineering, chemoenzymatic remodeling and site-specific glycosylation have attracted increasing interest. The therapeutic hormone erythropoietin (EPO) has been investigated in particular by various groups to establish a production process resulting in a defined glycosylation pattern. However commercially available recombinant human EPO shows batch-to-batch variations in its glycoforms. Therefore we present an alternative method for the synthesis of active glycosylated EPO with an engineered O-glycosylation site by combining eukaryotic cell-free protein synthesis and site-directed incorporation of non-canonical amino acids with subsequent chemoselective modifications.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 824
KW - recombinat-human-erythropoietin
KW - glycosylation
KW - expression
KW - site
KW - anemia
KW - CDNA
KW - glycoprotein
KW - purification
KW - cloning
KW - growth
Y1 - 2020
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-427017
IS - 824
ER -
TY - JOUR
A1 - Shahnejat-Bushehri, Sara
A1 - Allu, Annapurna Devi
A1 - Mehterov, Nikolay
A1 - Thirumalaikumar, Venkatesh P.
A1 - Alseekh, Saleh
A1 - Fernie, Alisdair R.
A1 - Mueller-Roeber, Bernd
A1 - Balazadeh, Salma
T1 - Arabidopsis NAC Transcription Factor JUNGBRUNNEN1 Exerts Conserved Control Over Gibberellin and Brassinosteroid Metabolism and Signaling Genes in Tomato
JF - Frontiers in plant science
N2 - The Arabidopsis thaliana NAC transcription factor JUNGBRUNNEN1 (AtJUB1) regulates growth by directly repressing GA3ox1 and DWF4, two key genes involved in gibberellin (GA) and brassinosteroid (BR) biosynthesis, respectively, leading to GA and BR deficiency phenotypes. AtJUB1 also reduces the expression of PIF4, a bHLH transcription factor that positively controls cell elongation, while it stimulates the expression of DELLA genes, which are important repressors of growth. Here, we extend our previous findings by demonstrating that AtJUB1 induces similar GA and BR deficiency phenotypes and changes in gene expression when overexpressed in tomato (Solanum lycopersicum). Importantly, and in accordance with the growth phenotypes observed, AtJUB1 inhibits the expression of growth-supporting genes, namely the tomato orthologs of GA3ox1, DWF4 and PIF4, but activates the expression of DELLA orthologs, by directly binding to their promoters. Overexpression of AtJUB1 in tomato delays fruit ripening, which is accompanied by reduced expression of several ripeningrelated genes, and leads to an increase in the levels of various amino acids (mostly proline, beta-alanine, and phenylalanine), gamma-aminobutyric acid (GABA), and major organic acids including glutamic acid and aspartic acid. The fact that AtJUB1 exerts an inhibitory effect on the GA/BR biosynthesis and PIF4 genes but acts as a direct activator of DELLA genes in both, Arabidopsis and tomato, strongly supports the model that the molecular constituents of the JUNGBRUNNEN1 growth control module are considerably conserved across species.
KW - Arabidopsis
KW - tomato
KW - fruit
KW - growth
KW - transcription factor
KW - gibberellic acid
KW - brassinosteroid
KW - DELLA proteins
Y1 - 2017
U6 - https://doi.org/10.3389/fpls.2017.00214
SN - 1664-462X
VL - 8
PB - Frontiers Research Foundation
CY - Lausanne
ER -
TY - JOUR
A1 - Rogol, Alan D.
ED - Scheffler, Christiane
ED - Koziel, Slawomir
ED - Hermanussen, Michael
ED - Bogin, Barry
T1 - Settings Perspective
BT - Bridging the Gap between Human Biology and Public Health
T2 - Human Biology and Public Health
KW - growth
KW - maturation
KW - populations
KW - secular change
Y1 - 2021
U6 - https://doi.org/10.52905/hbph.v1.2
SN - 2748-9957
VL - 2021
IS - 1
SP - 1
EP - 2
PB - Universitätsverlag Potsdam
CY - Potsdam
ER -
TY - JOUR
A1 - Ribeiro, Dimas M.
A1 - Araujo, Wagner L.
A1 - Fernie, Alisdair R.
A1 - Schippers, Jos H. M.
A1 - Müller-Röber, Bernd
T1 - Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis
JF - Journal of experimental botany
N2 - Although gibberellins (GAs) are well known for their growth control function, little is known about their effects on primary metabolism. Here the modulation of gene expression and metabolic adjustment in response to changes in plant (Arabidopsis thaliana) growth imposed on varying the gibberellin regime were evaluated. Polysomal mRNA populations were profiled following treatment of plants with paclobutrazol (PAC), an inhibitor of GA biosynthesis, and gibberellic acid (GA(3)) to monitor translational regulation of mRNAs globally. Gibberellin levels did not affect levels of carbohydrates in plants treated with PAC and/or GA(3). However, the tricarboxylic acid cycle intermediates malate and fumarate, two alternative carbon storage molecules, accumulated upon PAC treatment. Moreover, an increase in nitrate and in the levels of the amino acids was observed in plants grown under a low GA regime. Only minor changes in amino acid levels were detected in plants treated with GA(3) alone, or PAC plus GA(3). Comparison of the molecular changes at the transcript and metabolite levels demonstrated that a low GA level mainly affects growth by uncoupling growth from carbon availability. These observations, together with the translatome changes, reveal an interaction between energy metabolism and GA-mediated control of growth to coordinate cell wall extension, secondary metabolism, and lipid metabolism.
KW - Gibberellin
KW - growth
KW - paclobutrazol
KW - primary metabolism
KW - translatome
Y1 - 2012
U6 - https://doi.org/10.1093/jxb/err463
SN - 0022-0957
VL - 63
IS - 7
SP - 2769
EP - 2786
PB - Oxford Univ. Press
CY - Oxford
ER -
TY - THES
A1 - Pietzker, Christian
T1 - In-situ Wachstumsuntersuchungen beim reaktiven Anlassen von Cu, In Schichten in elementarem Schwefel
N2 - In dieser Arbeit wurde das reaktive Anlassen von dünnen Kupfer-Indium-Schichten in elementarem Schwefel mittels energiedispersiver Röntgenbeugung untersucht. Durch die simultane Aufnahme der Röntgenspektren und der Messung der diffusen Reflexion von Laserlicht der Wellenlänge 635 nm an der Oberfläche der Probe während des Schichtwachstums von CuInS2 konnte eine Methode zur Prozesskontrolle für ein Herstellungsverfahren von CuInS2 etabliert werden. Die Bildung von CuInS2 aus Kupfer-Indium-Vorläuferschichten wird dominiert von Umwandlungen der intermetallischen Phasen. CuInS2 wächst innerhalb der Aufheizperiode ab einer Temperatur von ca. 200°C aus der Phase Cu11In9. Jedoch zerfällt letztere metallische Phase in Cu16In9 und flüssiges Indium bei einer Temperatur von ca. 310°C. Das flüssige Indium reagiert im Falle von Kupferarmut mit dem Schwefel und führt zu einem zusätzlichen Reaktionspfad über InS zu CuIn5S8. Unter Präparationsbedingungen mit Kupferüberschuss wird das Indium in einer intermetallischen Phase gebunden.Erstmals konnte die Phase Digenite bei Temperaturen über 240°C beobachtet werden. Beim Abkühlen auf Raumtemperatur wandelt sich diese Phase unter dem Verbrauch von Schwefel in Covellite um.Für Proben mit Kupferüberschuss konnte eine Wachstumskinetik proportional zur Temperatur beobachtet werden. Dieses Verhalten wurde durch eine stress-induzierte Diffusion als dominierenden Reaktionsmechanismus interpretiert. Dabei werden während der Bildung von CuInS2 durch unterschiedliche Ausdehnungen der metallischen und sulfidischen Schichten eine Spannung in der CuInS2-Schicht induziert, die nach Überschreiten einer Grenzspannung zu Rissen in der CuInS2-Schicht führt. Entlang dieser Risse findet ein schneller Transport der Metalle zur Oberfläche, wo diese mit dem Schwefel reagieren können, statt. Die Risse heilen durch die Bildung neuen Sulfids wieder aus.
N2 - In this work the reactive annealing of thin copper and indium films in elemental sulphur was investigated by energy dispersive X-ray diffraction. Measuring simultanously laser light diffusively reflected from the growth surface, a simple method for process monitoring could be established. The process monitoring using 635 nm laser light can now independently be used in production.The growth of CuInS2 from copper-indium precursors is dominated by transitions between intermetallic phases. CuInS2 growths in the heat up period above 200 °C from the phase Cu11In9. However the latter metallic phase decomposes into Cu16In9 and liquid indium at a temperature of 310 °C. The liquid indium reacts in the case of copper deficiency with sulphur to InS. This leads to an additional reaction path via InS to CuIn5S8. Under preparation conditions with copper excess to the contrary, indium is bound in an intermetallic phase.For the first time the phase Digenite could be observed in a growth experiment at temperatures above 240 °C. During cool down to room temperature this phase transforms to Covellite by consumption of sulphur.For samples with copper excess a growth kinetic proportional to the temperature was observed. This behaviour is interpreted by a stress induced reaction mechanism: During the formation of CuInS2, strain in the CuInS2 thin film is induced due to different expansion coefficients of the metallic and sulphurous phases. After transgression of a certain strain limit, cracks within CuInS2 are formed. Along these cracks, fast transport of metallic species to the surface can occur. There these species can react with the sulphur. The cracks can heal up by the formation of new sulphides.
KW - CuInS2
KW - Chalkopyrit
KW - Wachstum
KW - EDXRD
KW - LLS
KW - elementarer Schwefel
KW - reaktives Anlassen
KW - CuInS2
KW - Chalcopyrite
KW - growth
KW - EDXRD
KW - LLS
KW - elemental sulphur
KW - reactive annealing
Y1 - 2003
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-0001219
ER -
TY - JOUR
A1 - Petrov, Veselin
A1 - Schippers, Jos
A1 - Benina, Maria
A1 - Minkov, Ivan
A1 - Müller-Röber, Bernd
A1 - Gechev, Tsanko S.
T1 - In search for new players of the oxidative stress network by phenotyping an Arabidopsis T-DNA mutant collection on reactive oxygen species-eliciting chemicals
JF - Plant omics
N2 - The ability of some chemical compounds to cause oxidative stress offers a fast and convenient way to study the responses of plants to reactive oxygen species (ROS). In order to unveil potential novel genetic players of the ROS-regulatory network, a population of similar to 2,000 randomly selected Arabidopsis thaliana T-DNA insertion mutants was screened for ROS sensitivity/resistance by growing seedlings on agar medium supplemented with stress-inducing concentrations of the superoxide-eliciting herbicide methyl viologen or the catalase inhibitor 3-amino-triazole. A semi-robotic setup was used to capture and analyze images of the chemically treated seedlings which helped interpret the screening results by providing quantitative information on seedling area and healthy-to-chlorotic tissue ratios for data verification. A ROS-related phenotype was confirmed in three of the initially selected 33 mutant candidates, which carry T-DNA insertions in genes encoding a Ring/Ubox superfamily protein, ABI5 binding protein 1 (AFP1), previously reported to be involved in ABA signaling, and a protein of unknown function, respectively. In addition, we identified six mutants, most of which have not been described yet, that are related to growth or chloroplast development and show defects in a ROS-independent manner. Thus, semi-automated image capturing and phenotyping applied on publically available T-DNA insertion collections adds a simple means for discovering novel mutants in complex physiological processes and identifying the genes involved.
KW - growth
KW - image analysis
KW - methyl viologen
KW - LemnaTec
KW - screening
KW - superoxide
Y1 - 2013
SN - 1836-0661
VL - 6
IS - 1
SP - 46
EP - 54
PB - Southern Cross Publ.
CY - Lismore
ER -
TY - JOUR
A1 - Olas, Justyna Jadwiga
A1 - Fichtner, Franziska
A1 - Apelt, Federico
T1 - All roads lead to growth
BT - imaging-based and biochemical methods to measure plant growth
JF - Journal of experimental botany
N2 - Plant growth is a highly complex biological process that involves innumerable interconnected biochemical and signalling pathways. Many different techniques have been developed to measure growth, unravel the various processes that contribute to plant growth, and understand how a complex interaction between genotype and environment determines the growth phenotype. Despite this complexity, the term 'growth' is often simplified by researchers; depending on the method used for quantification, growth is viewed as an increase in plant or organ size, a change in cell architecture, or an increase in structural biomass. In this review, we summarise the cellular and molecular mechanisms underlying plant growth, highlight state-of-the-art imaging and non-imaging-based techniques to quantitatively measure growth, including a discussion of their advantages and drawbacks, and suggest a terminology for growth rates depending on the type of technique used.
KW - biomass
KW - growth
KW - imaging
KW - kinematics
KW - morphometrics
KW - phenomics
KW - phenotyping
KW - relative expansion rate of growth (RER)
KW - relative growth
KW - rate (RGR)
Y1 - 2019
U6 - https://doi.org/10.1093/jxb/erz406
SN - 0022-0957
SN - 1460-2431
VL - 71
IS - 1
SP - 11
EP - 21
PB - Oxford Univ. Press
CY - Oxford
ER -
TY - JOUR
A1 - Lisso, Janina
A1 - Schröder, Florian
A1 - Müssig, Carsten
T1 - EXO modifies sucrose and trehalose responses and connects the extracellular carbon status to growth
JF - Frontiers in plant science
N2 - Plants have the capacity to adapt growth to changing environmental conditions. This implies the modulation of metabolism according to the availability of carbon (C). Particular interest in the response to the C availability is based on the increasing atmospheric levels of CO2. Several regulatory pathways that link the C status to growth have emerged. The extracellular EXO protein is essential for cell expansion and promotes shoot and root growth. Homologous proteins were identified in evolutionarily distant green plants. We show here that the EXO protein connects growth with C responses. The exo mutant displayed altered responses to exogenous sucrose supplemented to the growth medium. Impaired growth of the mutant in synthetic medium was associated with the accumulation of starch and anthocyanins, altered expression of sugar-responsive genes, and increased abscisic acid levels. Thus, EXO modulates several responses related to the C availability. Growth retardation on medium supplemented with 2-deoxy-glucose, mannose, and palatinose was similar to the wildtype. Trehalose feeding stimulated root growth and shoot biomass production of exoplants where as it inhibited growth of the wildtype. The phenotypic features of the exo mutant suggest that apoplastic processes coordinate growth and C responses.
KW - EXO
KW - growth
KW - sugar response
KW - trehalose
KW - apoplast
Y1 - 2013
U6 - https://doi.org/10.3389/fpls.2013.00219
SN - 1664-462X
VL - 4
IS - 25
PB - Frontiers Research Foundation
CY - Lausanne
ER -
TY - GEN
A1 - Lenhard, Michael
T1 - All's well that ends well
BT - arresting cell proliferation in leaves
T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2 - The transition from cell proliferation to cell expansion is critical for determining leaf size. Andriankaja et al. (2012) demonstrate that in leaves of dicotyledonous plants, a basal proliferation zone is maintained for several days before abruptly disappearing, and that chloroplast differentiation is required to trigger the onset of cell expansion.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 906
KW - arabidopsis-thaliana
KW - genetic-control
KW - growth
KW - size
KW - curvature
Y1 - 2020
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-438035
SN - 1866-8372
IS - 906
SP - 9
EP - 11
ER -
TY - GEN
A1 - Johnson, Kim L.
A1 - Ramm, Sascha
A1 - Kappel, Christian
A1 - Ward, Sally
A1 - Leyser, Ottoline
A1 - Sakamoto, Tomoaki
A1 - Kurata, Tetsuya
A1 - Bevan, Michael W.
A1 - Lenhard, Michael
T1 - The tinkerbell (tink) mutation identifies the dual-specificity MAPK phosphatase INDOLE- 3-BUTYRIC ACID-RESPONSE5 (IBR5) as a novel regulator of organ size in Arabidopsis
T2 - PLoS ONE
N2 - Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) that we named tinkerbell (tink) due to its small size. Analysis of the tink mutant indicates that IBR5 acts as a novel regulator of organ size that changes the rate of growth in petals and leaves. Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway. Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development. We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.
T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 427
KW - class-i
KW - protein phosphatase
KW - auxin
KW - responses
KW - thaliana
KW - kinase
KW - growth
KW - interacts
KW - distinct
KW - pathway
Y1 - 2018
U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-410245
ER -