TY - GEN A1 - Berger, Frauke A1 - Höhle, Barbara T1 - Restrictions on addition BT - children’s interpretation of the focus particles auch ‘ also ’ and nur ‘ only ’ in German T2 - Postprints der Universität Potsdam Humanwissenschaftliche Reihe N2 - Children up to school age have been reported to perform poorly when interpreting sentences containing restrictive and additive focus particles by treating sentences with a focus particle in the same way as sentences without it. Careful comparisons between results of previous studies indicate that this phenomenon is less pronounced for restrictive than for additive particles. We argue that this asymmetry is an effect of the presuppositional status of the proposition triggered by the additive particle. We tested this in two experiments with German-learning three-and four-year-olds using a method that made the exploitation of the information provided by the particles highly relevant for completing the task. Three-year-olds already performed remarkably well with sentences both with auch 'also' and with nur 'only'. Thus, children can consider the presuppositional contribution of the additive particle in their sentence interpretation and can exploit the restrictive particle as a marker of exhaustivity. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 509 KW - presupposition KW - comprehension KW - implicature KW - acquisition KW - semantics KW - asymmetry KW - tolerance KW - mandarin Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-414911 SN - 1866-8364 IS - 509 ER - TY - BOOK A1 - Krivochen, Diego Gabriel T1 - The Syntax and Semantics of the Nominal Construction BT - A Radically Minimalist Perspective T3 - Potsdam Linguistic Investigations Y1 - 2012 SN - 978-3-631-62448-7 VL - 8 PB - Peter Lang CY - Frankfurt am Main ER - TY - JOUR A1 - Günther, Oliver T1 - More politics in the GI - More GI in politics JF - Informatik-Spektrum Y1 - 2012 U6 - https://doi.org/10.1007/s00287-012-0657-9 VL - 35 IS - 6 SP - 397 EP - 398 ER - TY - CHAP A1 - Schöndienst, Valentin A1 - Kulzer, Florian A1 - Günther, Oliver T1 - Like versus dislike BT - How Facebook's like-button influences people's perception of product and service quality T2 - International Conference on Information Systems : (ICIS 2012) N2 - As Facebook's Like-button has become ubiquitous, it is the purpose of this research to investigate (1) whether Likes serve as a signal of a product's or service's quality and (2) how the introduction of a Dislike-button would alter perceptions. Following a qualitative study, we conducted an experiment in which 653 participants were presented with website screenshots featuring varying levels of Likes and Dislikes. The results indicate that the theoretical framing of Likes as a Signal is valid and that people do perceive the quality of products and services as superior when they are associated with more Likes. Signaling also explains the counter-intuitive finding that Dislikes can have a positive effect on people's quality perceptions. Results are discussed with respect to theory and practical implications. Y1 - 2012 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84886484949&partnerID=MN8TOARS SP - 439 EP - 454 PB - Curran CY - Red Hook ER - TY - JOUR A1 - Winkler, Till J. A1 - Goebel, Christoph A1 - Bidault, Francis A1 - Günther, Oliver T1 - Information technology and business practieses in Germany BT - results from the 2011 bit survey JF - The UCLA Anderson Business and Information Technologies (BIT) Project Y1 - 2012 U6 - https://doi.org/10.1142/9789814390880_0005 SP - 81 EP - 114 PB - world scientific CY - Singapore ER - TY - CHAP A1 - Günther, Oliver A1 - Winkler, Till J. T1 - Explaining the governance of software as a service applications BT - A process view T2 - Multikonferenz Wirtschaftsinformatik 2012 - Tagungsband der MKWI 2012 N2 - Defining the allocation of decision rights for enterprise applications is a crucial issue in IT governance and organization design. Today, emerging delivery models such as Software as a Service (SaaS) defy the notion of the internal IT department as the focal point of centralized governance. Recognizing the importance of this issue, we find that the phenomenon of 'SaaS governance' itself is not yet well understood. Based on two cases of SaaS adoption, we take a process-theoretic approach to investigate the complex interaction between factors that influence in the allocation of SaaS authority. The results suggest that some factors, such as the locus of initiative and the decision for SaaS, interact with absorptive capacities and determine the later mode of application governance at a very early stage. Thus, the initiative for introducing SaaS emerges as an important intermediate variable between the overall IT governance mode and the resulting SaaS governance outcome. Y1 - 2012 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84879878424&partnerID=MN8TOARS SN - 978-3-942183-63-5 SP - 599 EP - 612 PB - Gito CY - Berlin ER - TY - JOUR A1 - Günther, Oliver A1 - Brecht, Franziska A1 - Eckhardt, Andreas A1 - Berger, Christian T1 - Corporate career presences on social network sites: an analysis of hedonic and utilitarian value JF - CHI '12 Proceedings of the SIGCHI Conference on Human Factors in Computing Systems N2 - Due to the shortage of skilled workforce and the increasing usage of social network sites, companies increasingly apply social network sites to attract potential applicants. This paper explores how corporate career presences on network sites should be realized in order to attract potential applicants. Therefore, we tested the impact of seven individual characteristics (namely Appointments, Daily Working Routine, Jobs, Corporate News, Entertainment, Media Format, and Features) of these corporate career presences that we extracted by a comprehensive pre-study on users' perceived hedonic and utilitarian value of these presences on social network sites. Based on an online survey with 470 participants, the results reveal a highly significant impact of five characteristics that corporate career presences provide both a hedonic as well as a utilitarian value to the user Y1 - 2012 SN - 978-1-4503-1015-4 U6 - https://doi.org/10.1145/2207676.2208408 SP - 2441 EP - 2450 PB - ACM CY - Texas ER - TY - GEN A1 - Festman, Julia T1 - Language control abilities of late bilinguals T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Although all bilinguals encounter cross-language interference (CLI), some bilinguals are more susceptible to interference than others. Here, we report on language performance of late bilinguals (Russian/German) on two bilingual tasks (interview, verbal fluency), their language use and switching habits. The only between-group difference was CLI: one group consistently produced significantly more errors of CLI on both tasks than the other (thereby replicating our findings from a bilingual picture naming task). This striking group difference in language control ability can only be explained by differences in cognitive control, not in language proficiency or language mode. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 459 KW - language control KW - language proficiency KW - interference KW - error analysis KW - language mode KW - switching attitude Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-413930 IS - 459 ER - TY - JOUR A1 - Stadler, Heike T1 - Sich der Diskussion stellen BT - eine Online-Petition sorgt schnell für Aufsehen : erfolgreiche Beispiele JF - BuB : Forum für Bibliothek und Information 64 (2012) 4 N2 - Die Zukunftswerkstatt wollte wissen, ob Konzepte wie Liquid Democracy (http://liqd.net/) und Plattformen wie Adhocracy (http://code.adhocracy.de/) oder AVAAZ (www.avaaz.org/ de/) konkret für das Bibliothekswesen eingesetzt werden können. Die Beteiligung an der Internet-Enquete Kommission des Bundestages (https://enquetebeteiligung. de/) zeigte, dass die Nutzung im Sinne von gemeinschaftlicher Politikberatung möglich ist. Die »Stop SOPA/PIPA«- und die »Anti-ACTA«-Mobilisierungen belegen, dass Politik immer mehr auf die Bürger eingehen muss – und Social Media mitnichten tot sind. Heike Stadler beschäftigt sich intensiv mit dem Thema »Bürgerbeteiligungen für Bibliotheken« (siehe unter anderem BuB Heft 6/2011, Seite 450). Hier ihr Beitrag für die Zukunftswerkstatt Y1 - 2012 UR - www.b-u-b.de/pdfarchiv/Heft-BuB_04_2012.pdf VL - 64 IS - 4 SP - 248 PB - Bock + Herchen CY - Bad Honnef ER - TY - GEN A1 - Salzwedel, Annett A1 - Nosper, Manfred A1 - Röhrig, Bernd A1 - Linck-Eleftheriadis, Sigrid A1 - Strandt, Gert A1 - Völler, Heinz T1 - Outcome quality of in-patient cardiac rehabilitation in elderly patients – identification of relevant parameters T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Background Outcome quality management requires the consecutive registration of defined variables. The aim was to identify relevant parameters in order to objectively assess the in-patient rehabilitation outcome. Methods From February 2009 to June 2010 1253 patients (70.9 ± 7.0 years, 78.1% men) at 12 rehabilitation clinics were enrolled. Items concerning sociodemographic data, the impairment group (surgery, conservative/interventional treatment), cardiovascular risk factors, structural and functional parameters and subjective health were tested in respect of their measurability, sensitivity to change and their propensity to be influenced by rehabilitation. Results The majority of patients (61.1%) were referred for rehabilitation after cardiac surgery, 38.9% after conservative or interventional treatment for an acute coronary syndrome. Functionally relevant comorbidities were seen in 49.2% (diabetes mellitus, stroke, peripheral artery disease, chronic obstructive lung disease). In three key areas 13 parameters were identified as being sensitive to change and subject to modification by rehabilitation: cardiovascular risk factors (blood pressure, low-density lipoprotein cholesterol, triglycerides), exercise capacity (resting heart rate, maximal exercise capacity, maximal walking distance, heart failure, angina pectoris) and subjective health (IRES-24 (indicators of rehabilitation status): pain, somatic health, psychological well-being and depression as well as anxiety on the Hospital Anxiety and Depression Scale). Conclusion The outcome of in-patient rehabilitation in elderly patients can be comprehensively assessed by the identification of appropriate key areas, that is, cardiovascular risk factors, exercise capacity and subjective health. This may well serve as a benchmark for internal and external quality management. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 390 KW - cardiac rehabilitation KW - quality management KW - outcome measures Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-404151 IS - 390 ER - TY - GEN A1 - Wattendorf, Elise A1 - Festman, Julia A1 - Westermann, Birgit A1 - Keil, Ursula A1 - Zappatore, Daniela A1 - Franceschini, Rita A1 - Luedi, Georges A1 - Radue, Ernst-Wilhelm A1 - Münte, Thomas F. A1 - Rager, Günter A1 - Nitsch, Cordula T1 - Early bilingualism influences early and subsequently later acquired languages in cortical regions representing control functions T2 - Postprints der Universität Potsdam : Humanwissenschaftliche Reihe N2 - Early acquisition of a second language influences the development of language abilities and cognitive functions. In the present study, we used functional Magnetic Resonance Imaging (fMRI) to investigate the impact of early bilingualism on the organization of the cortical language network during sentence production. Two groups of adult multilinguals, proficient in three languages, were tested on a narrative task; early multilinguals acquired the second language before the age of three years, late multilinguals after the age of nine. All participants learned a third language after nine years of age. Comparison of the two groups revealed substantial differences in language-related brain activity for early as well as late acquired languages. Most importantly, early multilinguals preferentially activated a fronto-striatal network in the left hemisphere, whereas the left posterior superior temporal gyrus (pSTG) was activated to a lesser degree than in late multilinguals. The same brain regions were highlighted in previous studies when a non-target language had to be controlled. Hence the engagement of language control in adult early multilinguals appears to be influenced by the specific learning and acquisition conditions during early childhood. Remarkably, our results reveal that the functional control of early and subsequently later acquired languages is similarly affected, suggesting that language experience has a pervasive influence into adulthood. As such, our findings extend the current understanding of control functions in multilinguals. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - 384 KW - multilingual KW - language acquisition KW - narration KW - age of acquisition KW - functional magnetic resonance imaging KW - emergentist framework Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-404092 IS - 384 ER - TY - GEN A1 - Ganghof, Steffen T1 - Does public reason require super-majoritarian democracy? BT - Liberty, equality, and history in the justification of political institutions T2 - Politics, philosophy & economics N2 - The project of public-reason liberalism faces a basic problem: publicly justified principles are typically too abstract and vague to be directly applied to practical political disputes, whereas applicable specifications of these principles are not uniquely publicly justified. One solution could be a legislative procedure that selects one member from the eligible set of inconclusively justified proposals. Yet if liberal principles are too vague to select sufficiently specific legislative proposals, can they, nevertheless, select specific legislative procedures? Based on the work of Gerald Gaus, this article argues that the only candidate for a conclusively justified decision procedure is a majoritarian or otherwise ‘neutral’ democracy. If the justification of democracy requires an equality baseline in the design of political regimes and if justifications for departure from this baseline are subject to reasonable disagreement, a majoritarian design is justified by default. Gaus’s own preference for super-majoritarian procedures is based on disputable specifications of justified liberal principles. These procedures can only be defended as a sectarian preference if the equality baseline is rejected, but then it is not clear how the set of justifiable political regimes can be restricted to full democracies. T3 - Zweitveröffentlichungen der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe - 86 KW - public-reason liberalism KW - democracy KW - coercion KW - political equality KW - majority rule KW - Gerald Gaus Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-477860 VL - 12 IS - 2 ER - TY - THES A1 - Richter, Johanna T1 - Transformationen des Romans im Zeitalter der Presse BT - Paris und London: 1836-1881 Y1 - 2012 ER - TY - BOOK A1 - Thielicke, Hubert A1 - Birukov, Sergej A1 - Dornfeldt, Matthias ED - Winter-Hamerla, Heike T1 - Die Eurasische Union : Postsowjetischer Traum oder weitreichendes Integrationsprojekt? T1 - The Eurasian Union : Postsoviet dream or expansive project of integration? T3 - WeltTrends Spezial N2 - Während die EU eine schwere Krise erlebt, denkt Russlands Präsident Putin zusammen mit seinen Amtskollegen aus Belarus und Kasachstan laut über die Schaffung einer Eurasischen Union nach. Basierend auf bereits existierenden Organisationsformen im postsowjetischen Raum soll diese Vereinigung zur Brücke zwischen Europa und Asien werden. Welche Hürden dieses Integrationsprojekt überwinden muss und ob das Ganze auf eine Wiederbelebung der UdSSR hinausläuft, analysiert Sergej Birukov. Ergänzt wird das Spezial durch einen Konferenzbericht zum Berliner Eurasischen Klub sowie einem Beitrag zu Kasachstans Rolle bei der Schaffung der ersten kernwaffenfreien Zone in der nördlichen Hemisphäre. KW - Eurasiche Union KW - Russland KW - Belarus KW - Kasachstan KW - Eurasian Union KW - Russia KW - Belarus KW - Kazakhstan Y1 - 2012 SN - 978-3-941880-44-3 SN - 2193-0627 IS - 8 ER - TY - JOUR A1 - Röttgen, Norbert ED - Kleinwächter, Kai T1 - Regierungserklärung zu den Ergebnissen der Klimakonferenz in Durban BT - Bundesminister für Umwelt, Naturschutz und Reaktorsicherheit, Dr. Norbert Röttgen, am 16. Dezember 2011 (Auszüge) JF - Klimapolitik International KW - Klimapolitik KW - Klima KW - Durban 2011 KW - Klimakonferenz KW - NGO KW - Entwicklungspolitik KW - climate policy KW - climate KW - Climate Change Conference KW - development policy Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-81376 SN - 1868-6222 SN - 1868-6230 SP - 93 EP - 100 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Balderjahn, Ingo A1 - Peyer, Mathias T1 - Das Bewusstsein für fairen Konsum : Konzeptualisierung, Messung und Wirkung JF - Die Betriebswirtschaft : DBW N2 - Die vorliegende Arbeit fokussiert auf den fairen Konsum als Teil des ethischen Konsums. Unter fairem Konsum verstehen wir Kaufentscheidungen, die unter Berücksichtigung der Einhaltung fairer Arbeits- und Geschäftsbedingungen bei der Herstellung von Produkten erfolgen. Unter Einsatz einer neu entwickelten Skala zur Messung des fairen Konsumbewusstseins können wir empirisch nachweisen, dass Produkte mit einem Fairtrade-Siegel Konsumenten einen moralischen Zusatznutzen vermitteln können, für den sie bereit sind, einen Mehrpreis zu zahlen. N2 - The present paper focuses on fair consumption, as a part of ethical consumerism. Fair consumption means purchasing decisions made in the light of compliance with fair labor and business conditions in the manufacturing process. Using a new developed scale for the measurement of the consciousness for fair consumption, we can prove empirically that fair trade products can provide an additional moral value for consumers for which they are willingly to pay an extra charge. T2 - The consciousness for fair consumption : conceptualization, measuring, and impacts KW - Conjoint Analyse KW - Consumer Social Responsibility KW - ethischer Konsum KW - faires Konsumbewusstsein KW - Fairtrade KW - Strukturgleichungsmodelle KW - Zahlungsbereitschaft KW - Conjoint analysis KW - consciousness for fair consumption KW - consumer social responsibility KW - ethical consumerism KW - fairtrade KW - structural equation modeling KW - willingness-to-pay Y1 - 2012 SN - 0342-7064 VL - 72 IS - 4 SP - 343 EP - 364 PB - Schäffer-Poeschel CY - Stuttgart ER - TY - CHAP A1 - Balderjahn, Ingo A1 - Peyer, Mathias ED - Corsten, Hans ED - Roth, Stefan T1 - Soziales Konsumbewusstsein: Skalenentwicklung und –validierung T2 - Nachhaltigkeit – Unternehmerisches Handeln in globaler Verantwortung Y1 - 2012 SN - 978-3-8349-3179-5 SP - 93 EP - 112 PB - Springer Gabler CY - Wiesbaden ER - TY - JOUR A1 - Weiß, Norman ED - Breuer, Marten ED - Epinay, Astrid ED - Haratsch, Andreas ED - Schmahl, Stefanie ED - Weiß, Norman T1 - Der Rechtsstaat im Risiko JF - Der Staat im Recht : Festschrift für Eckart Klein zum 70. Geburtstag (Schriften zum öffentlichen Recht ; Bd. 1232) Y1 - 2012 SN - 978-3-428-13738-1 SP - 365 EP - 383 PB - Duncker & Humblot CY - Berlin ER - TY - JOUR A1 - Weiß, Norman T1 - Bedeutung und Funktion von Zivilgesellschaft und Öffentlichkeit im demokratischen Rechtsstaat am Beispiel der Bundesrepublik Deutschland JF - Jahrbuch des Öffentlichen Rechts der Gegenwart : Neue Folge ; 61 Y1 - 2012 UR - https://www.mohrsiebeck.com/buch/jahrbuch-des-oeffentlichen-rechts-der-gegenwart-neue-folge-9783161524172 SN - 978-3-16-152417-2 SN - 0075-2517 VL - 61 SP - 15 EP - 60 PB - Mohr Siebeck CY - Tübingen ER - TY - JOUR A1 - Pimpalpalle, Tukaram M. A1 - Yin, Jian A1 - Linker, Torsten T1 - Barton radical reactions of 2-C-branched carbohydrates JF - Organic & biomolecular chemistry : an international journal of synthetic, physical and biomolecular organic chemistry N2 - Barton esters have been introduced into the side chain of carbohydrates with high yields in only a few steps from easily available glycals. Their radical reactions afford 2-C-methyl and 2-C-bromomethyl hexoses, pentoses and disaccharides in good yields in analytically pure form. Since the Barton esters have been synthesized by an oxidative radical addition and their transformations by reductive radical processes, our results demonstrate the power of such reactions in carbohydrate chemistry. Y1 - 2012 U6 - https://doi.org/10.1039/c1ob06370g SN - 1477-0520 VL - 10 IS - 1 SP - 103 EP - 109 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Wang, Yongbo A1 - Liu, Xingqi A1 - Mischke, Steffen A1 - Herzschuh, Ulrike T1 - Environmental constraints on lake sediment mineral compositions from the Tibetan Plateau and implications for paleoenvironment reconstruction JF - Journal of paleolimnolog N2 - Inorganic minerals form a major component of lacustrine sediments and have the potential to reveal detailed information on previous climatic and hydrological conditions. The ability to extract such information however, has been restricted by a limited understanding of the relationships between minerals and the environment. In an attempt to fill in this gap in our knowledge, 146 surface sediment samples have been investigated from 146 lakes on the Tibetan Plateau. The mineral compositions derived from these samples by X-Ray Diffraction (XRD) were used to examine the relationships between mineral compositions and the environmental variables determined for each site. Statistical techniques including Multivariate regression trees (MRT) and Redundancy Analysis (RDA), based on the mineral spectra and environmental variables, reveal that the electrical conductivity (EC) and Mg/Ca ratios of lake water are the most important controls on the composition of endogenic minerals. No endogenic minerals precipitate under hyper-fresh water conditions (EC lower than 0.13 mS/cm), with calcite commonly forming in water with EC values above 0.13 mS/cm. Between EC values of 0.13 and 26 mS/cm the mineral composition of lake sediments can be explained in terms of variations in the Mg/Ca ratio: calcite dominates at Mg/Ca ratios of less than 33, whereas aragonite commonly forms when the ratio is greater than 33. Where EC values are between 26 and 39 mS/cm, monohydrocalcite precipitates together with calcite and aragonite; above 39 mS/cm, gypsum and halite commonly form. Information on the local geological strata indicates that allogenic (detrital) mineral compositions are primarily influenced by the bedrock compositions within the catchment area. By applying these relationships to the late glacial and Holocene mineral record from Chaka Salt Lake, five lake stages have been identified and their associated EC conditions inferred. The lake evolved from a freshwater lake during the late glacial (before 11.4 cal. ka BP) represented by the lowest EC values (< 0.13 mS/cm), to a saline lake with EC values slightly higher than 39 mS/cm during the early and mid Holocene (ca. 11.4-5.3 cal. ka BP), and finally to a salt lake (after 5.3 cal. ka BP). These results illustrate the utility of our mineral-environmental model for the quantitative reconstruction of past environmental conditions from lake sediment records. KW - Mineral composition KW - XRD KW - Multivariate regression trees KW - Electrical conductivity KW - Paleolimnology KW - Tibetan Plateau Y1 - 2012 U6 - https://doi.org/10.1007/s10933-011-9549-2 SN - 0921-2728 VL - 47 IS - 1 SP - 71 EP - 85 PB - Springer CY - Dordrecht ER - TY - CHAP A1 - Ohl, Sven A1 - Brandt, S. A1 - Kliegl, Reinhold T1 - Post-saccadic location judgments after presentation of multiple target-like objects T2 - Perception Y1 - 2012 SN - 0301-0066 SN - 1468-4233 VL - 41 IS - 1 SP - 171 EP - 171 PB - Sage Publ. CY - London ER - TY - CHAP A1 - Laubrock, Jochen A1 - Cajar, Anke A1 - Engbert, Ralf T1 - Peripheral spatial frequency processing affects timing and metrics of saccades T2 - Perception Y1 - 2012 SN - 0301-0066 SN - 1468-4233 VL - 41 SP - 170 EP - 170 PB - Sage Publ. CY - London ER - TY - CHAP A1 - Krügel, Andre A1 - Engbert, Ralf T1 - Saccade targeting of spatially extended objects a Bayesian model T2 - Perception Y1 - 2012 SN - 0301-0066 SN - 1468-4233 VL - 41 SP - 167 EP - 167 PB - Sage Publ. CY - London ER - TY - CHAP A1 - Risse, Sarah T1 - Letter crowding and the benefit of parafoveal preview during reading T2 - Perception Y1 - 2012 SN - 0301-0066 SN - 1468-4233 VL - 41 SP - 133 EP - 133 PB - Sage Publ. CY - London ER - TY - CHAP A1 - Trukenbrod, Hans Arne A1 - Barthelme, S. A1 - Wichmann, Felix A. A1 - Engbert, Ralf T1 - Color does not guide eye movements: Evidence from a gaze-contingent experiment T2 - PERCEPTION Y1 - 2012 SN - 0301-0066 SN - 1468-4233 VL - 41 SP - 88 EP - 88 PB - PION LTD CY - LONDON ER - TY - CHAP A1 - Engbert, Ralf T1 - A computational model of microsaccadic responses to shifts of covert attention T2 - Perception Y1 - 2012 SN - 0301-0066 SN - 1468-4233 VL - 41 SP - 55 EP - 55 PB - Sage Publ. CY - London ER - TY - JOUR A1 - Hildebrandt, Niko A1 - Geissler, Daniel ED - Zahavy, E ED - Ordentlich, A ED - Yitzhaki, S ED - Shafferman, A T1 - Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application JF - Advances in Experimental Medicine and Biology N2 - Applications based on Forster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements. KW - Diagnostics KW - FRET KW - Imaging KW - Quantum dots KW - Terbium Y1 - 2012 SN - 978-94-007-2554-6 SN - 978-94-007-2555-3 U6 - https://doi.org/10.1007/978-94-007-2555-3_8 SN - 0065-2598 VL - 733 SP - 75 EP - 86 PB - Springer CY - Dordrecht ER - TY - JOUR A1 - Wellmann, Caroline A1 - Holzgrefe-Lang, Julia A1 - Truckenbrodt, Hubert A1 - Wartenburger, Isabell A1 - Höhle, Barbara T1 - How each prosodic boundary cue matters evidence from German infants JF - Frontiers in psychology N2 - Previous studies have revealed that infants aged 6-10 months are able to use the acoustic correlates of major prosodic boundaries, that is, pitch change, preboundary lengthening, and pause, for the segmentation of the continuous speech signal. Moreover, investigations with American-English- and Dutch-learning infants suggest that processing prosodic boundary markings involves a weighting of these cues. This weighting seems to develop with increasing exposure to the native language and to underlie crosslinguistic variation. In the following, we report the results of four experiments using the headturn preference procedure to explore the perception of prosodic boundary cues in German infants. We presented 8-month-old infants with a sequence of names in two different prosodic groupings, with or without boundary markers. Infants discriminated both sequences when the boundary was marked by all three cues (Experiment 1) and when it was marked by a pitch change and preboundary lengthening in combination (Experiment 2). The presence of a pitch change (Experiment 3) or preboundary lengthening (Experiment 4) as single cues did not lead to a successful discrimination. Our results indicate that pause is not a necessary cue for German infants. Pitch change and preboundary lengthening in combination, but not as single cues, are sufficient. Hence, by 8 months infants only rely on a convergence of boundary markers. Comparisons with adults' performance on the same stimulus materials suggest that the pattern observed with the 8-month-olds is already consistent with that of adults. We discuss our findings with respect to crosslinguistic variation and the development of a language-specific prosodic cue weighting. KW - infants KW - language acquisition KW - speech perception KW - prosodic bootstrapping KW - prosodic boundary cues KW - cue weighting KW - intonation phrase boundary KW - headturn preference procedure Y1 - 2012 U6 - https://doi.org/10.3389/fpsyg.2012.00580 SN - 1664-1078 VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Henrichs, Ivanina A1 - Elsner, Claudia A1 - Elsner, Birgit A1 - Gredeback, Gustaf T1 - Goal salience affects infants' goal-directed gaze shifts JF - Frontiers in psychology N2 - Around their first year of life, infants are able to anticipate the goal of others' ongoing actions. For instance, 12-month-olds anticipate the goal of everyday feeding actions and manual actions such as reaching and grasping. However, little is known whether the salience of the goal influences infants' online assessment of others' actions. The aim of the current eye-tracking study was to elucidate infants' ability to anticipate reaching actions depending on the visual salience of the goal object. In Experiment 1, 12-month-old infants' goal-directed gaze shifts were recorded as they observed a hand reaching for and grasping either a large (high-salience condition) or a small (low-salience condition) goal object. Infants exhibited predictive gaze shifts significantly earlier when the observed hand reached for the large goal object compared to when it reached for the small goal object. In addition, findings revealed rapid learning over the course of trials in the high-salience condition and no learning in the low-salience condition. Experiment 2 demonstrated that the results could not be simply attributed to the different grip aperture of the hand used when reaching for small and large objects. Together, our data indicate that by the end of their first year of life, infants rely on information about the goal salience to make inferences about the action goal. KW - anticipation KW - eye movement KW - salience KW - infant KW - action understanding Y1 - 2012 U6 - https://doi.org/10.3389/fpsyg.2012.00391 SN - 1664-1078 VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - INPR A1 - Fischer, Martin H. A1 - Kaufmann, Liane A1 - Domahs, Frank T1 - Finger counting and numerical cognition T2 - Frontiers in psychology Y1 - 2012 U6 - https://doi.org/10.3389/fpsyg.2012.00108 SN - 1664-1078 VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Festman, Julia A1 - Muente, Thomas F. T1 - Cognitive control in Russian-German bilinguals JF - Frontiers in psychology N2 - Bilingual speakers are faced with the problem to keep their languages apart, but do so with interindividually varying success. Cognitive control abilities might be an important factor to explain such interindividual differences. Here we compare two late, balanced and highly proficient bilingual groups (mean age 24 years, L1 Russian, L2 German) which were established according to their language control abilities on a bilingual picture-naming task. One group had difficulties to remain in the instructed target language and switched unintentionally to the non-target language ("switchers"), whereas the other group rarely switched unintentionally ("non-switchers"). This group-specific behavior could not be explained by language background, socio-cultural, or demographic variables. Rather, the non-switchers also demonstrated a faster and better performance on four cognitive control tests (Tower of Hanoi, Ruff Figural Fluency Test, Divided Attention, Go/Nogo). Here, we focus on two additional executive function tasks, theWisconsin Card SortingTest (WCST) and the Flanker task requiring conflict monitoring and conflict resolution. Non-switchers outperformed switchers with regard to speed and accuracy, and were better at finding and applying the correct rules in the WCST. Similarly, in the Flanker task non-switchers performed faster and better on conflict trials and had a higher correction rate following an error. Event-related potential recordings furthermore revealed a smaller error-related negativity in the non-switchers, taken as evidence for a more efficient self-monitoring system. We conclude that bilingual language performance, in particular switching behavior, is related to performance on cognitive control tasks. Better cognitive control, including conflict monitoring, results in decreased unintentional switching. KW - Flanker task KW - ERN KW - Wisconsin Card Sorting Test KW - conflict monitoring KW - inhibition KW - late bilinguals KW - cognitive control KW - executive function Y1 - 2012 U6 - https://doi.org/10.3389/fpsyg.2012.00115 SN - 1664-1078 VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Domahs, Ulrike A1 - Knaus, Johannes A1 - Orzechowska, Paula A1 - Wiese, Richard T1 - Stress "deafness" in a language with fixed word stress an ERR study on Polish JF - Frontiers in psychology N2 - The aim of the present contribution was to examine the factors influencing the prosodic processing in a language with predictable word stress. For Polish, a language with fixed penultimate stress but several well-defined exceptions, difficulties in the processing and representation of prosodic information have been reported (e.g., Peperkamp and Dupoux, 2002). The present study utilized event-related potentials (ERPs) to investigate the factors influencing prosodic processing in Polish. These factors are (i) the predictability of stress and (ii) the prosodic structure in terms of metrical feet. Polish native speakers were presented with correctly and incorrectly stressed Polish words and instructed to judge the correctness of the perceived stress patterns. For some stress violations, an early negativity was found which was interpreted as a reflection of an error-detection mechanism. In addition, exceptional stress patterns (=antepenultimate stress) and post-lexical (=initial) stress evoked a task-related positivity effect (P300) whose amplitude and latency is correlated with the degree of anomaly and deviation from an expectation. In contrast, violations involving the default (=penultimate stress) did not produce such an effect. This asymmetrical result is interpreted to reflect that Polish native speakers are less sensitive to the default pattern than to the exceptional or post-lexical patterns. Behavioral results are orthogonal to the electrophysiological results showing that Polish speakers had difficulties to reject any kind of stress violation. Thus, on a meta-linguistic level Polish speakers appeared to be stress-"deaf" for any kind of stress manipulation, whereas the neural reactions differentiate between the default and lexicalized patterns. KW - stress "deafness" KW - fixed stress system KW - prosodic representation KW - P300 KW - generalized error-detection mechanism Y1 - 2012 U6 - https://doi.org/10.3389/fpsyg.2012.00439 SN - 1664-1078 VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Rocchetti, Alessandra A1 - Sharma, Tripti A1 - Wulfetange, Camilla A1 - Scholz-Starke, Joachim A1 - Grippa, Alexandra A1 - Carpaneto, Armando A1 - Dreyer, Ingo A1 - Vitale, Alessandro A1 - Czempinski, Katrin A1 - Pedrazzini, Emanuela T1 - The putative K+ channel subunit AtKCO3 forms stable dimers in arabidopsis JF - Frontiers in plant science N2 - The permeation pore of K+ channels is formed by four copies of the pore domain. AtKCO3 is the only putative voltage-independent K+ channel subunit of Arabidopsis thaliana with a single pore domain. KCO3-like proteins recently emerged in evolution and, to date, have been found only in the genus Arabidopsis (A. thaliana and A. lyrata). We show that the absence of KCO3 does not cause marked changes in growth under various conditions. Only under osmotic stress we observed reduced root growth of the kco3-1 null-allele line. This phenotype was complemented by expressing a KCO3 mutant with an inactive pore, indicating that the function of KCO3 under osmotic stress does not depend on its direct ability to transport ions. Constitutively overexpressed AtKCO3 or AtKCO3::G FP are efficiently sorted to the tonoplast indicating that the protein is approved by the endoplasmic reticulum quality control. However, vacuoles isolated from transgenic plants do not have significant alterations in current density. Consistently, both AtKCO3 and AtKCO3::GFP are detected as homodimers upon velocity gradient centrifugation, an assembly state that would not allow for activity. We conclude that if AtKCO3 ever functions as a K+ channel, active tetramers are held by particularly weak interactions, are formed only in unknown specific conditions and may require partner proteins. KW - Arabidopsis KW - membrane proteins KW - potassium channels KW - protein assembly KW - tonoplast Y1 - 2012 U6 - https://doi.org/10.3389/fpls.2012.00251 SN - 1664-462X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Lucia Gomez-Porras, Judith A1 - Mauricio Riano-Pachon, Diego A1 - Benito, Begona A1 - Haro, Rosario A1 - Sklodowski, Kamil A1 - Rodriguez-Navarro, Alonso A1 - Dreyer, Ingo T1 - Phylogenetic analysis of K+ transporters in bryophytes, lycophytes, and flowering plants indicates a specialization of vascular plants JF - Frontiers in plant science N2 - As heritage from early evolution, potassium (K+) is absolutely necessary for all living cells. It plays significant roles as stabilizer in metabolism and is important for enzyme activation, stabilization of protein synthesis, and neutralization of negative charges on cellular molecules as proteins and nucleic acids. Land plants even enlarged this spectrum of K+ utilization after having gone ashore, despite the fact that K+ is far less available in their new oligotrophic habitats than in sea water. Inevitably, plant cells had to improve and to develop unique transport systems for K+ accumulation and distribution. In the past two decades a manifold of K+ transporters from flowering plants has been identified at the molecular level. The recently published genome of the fern ally Selaginella moellendorffii now helps in providing a better understanding on the molecular changes involved in the colonization of land and the development of the vasculature and the seeds. In this article we present an inventory of K+ transporters of this lycophyte and pigeonhole them together with their relatives from the moss Physcomitrella patens, the monocotyledon Oryza sativa, and two dicotyledonous species, the herbaceous plant Arabidopsis thaliana, and the tree Populus trichocarpa. Interestingly, the transition of green plants from an aqueous to a dry environment coincides with a dramatic reduction in the diversity of voltage-gated potassium channels followed by a diversification on the basis of one surviving K+ channel class. The first appearance of K+ release (K-out) channels in S. moellendorffii that were shown in Arabidopsis to be involved in xylem loading and guard cell closure coincides with the specialization of vascular plants and may indicate an important adaptive step. KW - potassium KW - transport KW - channel KW - voltage-dependent KW - voltage-independent KW - high-affinity KW - Selaginella Y1 - 2012 U6 - https://doi.org/10.3389/fpls.2012.00167 SN - 1664-462X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Christian, Jan-Ole A1 - Braginets, Rostyslav A1 - Schulze, Waltraud X. A1 - Walther, Dirk T1 - Characterization and prediction of protein phosphorylation hotspots in Arabidopsis thaliana JF - Frontiers in plant science N2 - The regulation of protein function by modulating the surface charge status via sequence-locally enriched phosphorylation sites (P-sites) in so called phosphorylation "hotspots" has gained increased attention in recent years. We set out to identify P-hotspots in the model plant Arabidopsis thaliana. We analyzed the spacing of experimentally detected P-sites within peptide-covered regions along Arabidopsis protein sequences as available from the PhosPhAt database. Confirming earlier reports (Schweiger and Lanial, 2010), we found that, indeed, P-sites tend to cluster and that distributions between serine and threonine P-sites to their respected closest next P-site differ significantly from those for tyrosine P-sites. The ability to predict P-hotspots by applying available computational P-site prediction programs that focus on identifying single P-sites was observed to be severely compromised by the inevitable interference of nearby P-sites. We devised a new approach, named HotSPotter, for the prediction of phosphorylation hotspots. HotSPotter is based primarily on local amino acid compositional preferences rather than sequence position-specific motifs and uses support vector machines as the underlying classification engine. HotSPotter correctly identified experimentally determined phosphorylation hotspots in A. thaliana with high accuracy. Applied to the Arabidopsis proteome, HotSPotter-predicted 13,677 candidate P-hotspots in 9,599 proteins corresponding to 7,847 unique genes. Hotspot containing proteins are involved predominantly in signaling processes confirming the surmised modulating role of hotspots in signaling and interaction events. Our study provides new bioinformatics means to identify phosphorylation hotspots and lays the basis for further investigating novel candidate P-hotspots. All phosphorylation hotspot annotations and predictions have been made available as part of the PhosPhAt database at http://phosphat.mpimp-golm.mpg.de. KW - protein phosphorylation KW - hotspots KW - Arabidopsis thaliana KW - support vector machines KW - regulation Y1 - 2012 U6 - https://doi.org/10.3389/fpls.2012.00207 SN - 1664-462X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Balazadeh, Salma A1 - Jaspert, Nils A1 - Arif, Muhammad A1 - Müller-Röber, Bernd A1 - Maurino, Veronica G. T1 - Expression of ROS-responsive genes and transcription factors after metabolic formation of H2O2 in chloroplasts JF - Frontiers in plant science N2 - Glycolate oxidase (GO) catalyses the oxidation of glycolate to glyoxylate, thereby consuming O-2 and producing H2O2. In this work, Arabidopsis thaliana plants expressing GO in the chloroplasts (GO plants) were used to assess the expressional behavior of reactive oxygen species (ROS)-responsive genes and transcription factors (TFs) after metabolic induction of H2O2 formation in chloroplasts. In this organelle, GO uses the glycolate derived from the oxygenase activity of RubisCO. Here, to identify genes responding to an abrupt production of H2O2 in chloroplasts we used quantitative real-time PCR (qRT-PCR) to test the expression of 187 ROS-responsive genes and 1880 TFs after transferring GO and wild-type (WT) plants grown at high CO2 levels to ambient CO2 concentration. Our data revealed coordinated expression changes of genes of specific functional networks 0.5 h after metabolic induction of H2O2 production in GO plants, including the induction of indole glucosinolate and camalexin biosynthesis genes. Comparative analysis using available microarray data suggests that signals for the induction of these genes through H2O2 may originate in the chloroplast. The TF profiling indicated an up-regulation in GO plants of a group of genes involved in the regulation of proanthocyanidin and anthocyanin biosynthesis. Moreover, the upregulation of expression of IF and IF interacting proteins affecting development (e.g., cell division, stem branching, flowering time, flower development) would impact growth and reproductive capacity, resulting in altered development under conditions that promote the formation of H2O2. KW - glycolate oxidase KW - H2O2 KW - ROS-responsive genes KW - transcription factors Y1 - 2012 U6 - https://doi.org/10.3389/fpls.2012.00234 SN - 1664-462X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Spijkerman, Elly A1 - Wacker, Alexander A1 - Weithoff, Guntram A1 - Leya, Thomas T1 - Elemental and fatty acid composition of snow algae in Arctic habitats JF - Frontiers in microbiology N2 - Red, orange or green snow is the macroscopic phenomenon comprising different eukaryotic algae. Little is known about the ecology and nutrient regimes in these algal communities. Therefore, eight snow algal communities from five intensively tinted snow fields in western Spitsbergen were analysed for nutrient concentrations and fatty acid (FA) composition. To evaluate the importance of a shift from green to red forms on the FA-variability of the field samples, four snow algal strains were grown under nitrogen replete and moderate light (+N+ML) or N-limited and high light (-N+HL) conditions. All eight field algal communities were dominated by red and orange cysts. Dissolved nutrient concentration of the snow revealed a broad range of NH4+ (<0.005-1.2 mg NI-1) and only low PO43- (< 18 mu g P I-1) levels. The external nutrient concentration did not reflect cellular nutrient ratios as C:N and C:P ratios of the communities were highest at locations containing relatively high concentrations of NH4- and PO43-. Molar N:P ratios ranged from 11 to 21 and did not suggest clear limitation of a single nutrient. On a per carbon basis, we found a 6-fold difference in total FA content between the eight snow algal communities, ranging from 50 to 300 mg FA g C-1. In multivariate analyses total FA content opposed the cellular N:C quota and a large part of the FA variability among field locations originated from the abundant FAs C181n-9, C18 2n-6, and C183n-3. Both field samples and snow algal strains grown under -N+HL conditions had high concentrations of C181n-9. FAs possibly accumulated due to the cessation of growth. Differences in color and nutritional composition between patches of snow algal communities within one snow field were not directly related to nutrient conditions. We propose that the highly patchy distribution of snow algae within and between snow fields may also result from differences in topographical and geological parameters such as slope, melting water rivulets, and rock formation. KW - Arctic snow algal bloom KW - cellular C:N:P ratio KW - ecology KW - extremophiles KW - lipids KW - nutrients KW - psychrophilic. KW - Spitsbergen Y1 - 2012 U6 - https://doi.org/10.3389/fmicb.2012.00380 SN - 1664-302X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Sauer, Patrick A1 - Glombitza, Clemens A1 - Kallmeyer, Jens T1 - A system for incubations at high gas partial pressure JF - Frontiers in microbiology N2 - High-pressure is a key feature of deep subsurface environments. High partial pressure of dissolved gasses plays an important role in microbial metabolism, because thermodynamic feasibility of many reactions depends on the concentration of reactants. For gases, this is controlled by their partial pressure, which can exceed 1 MPa at in situ conditions. Therefore, high hydrostatic pressure alone is not sufficient to recreate true deep subsurface in situ conditions, but the partial pressure of dissolved gasses has to be controlled as well. We developed an incubation system that allows for incubations at hydrostatic pressure up to 60 MPa, temperatures up to 120 degrees C, and at high gas partial pressure. The composition and partial pressure of gasses can be manipulated during the experiment. To keep costs low, the system is mainly made from off-the-shelf components with only very few custommade parts. A flexible and inert PVDF (polyvinylidene fluoride) incubator sleeve, which is almost impermeable for gases, holds the sample and separates it from the pressure fluid. The flexibility of the incubator sleeve allows for sub-sampling of the medium without loss of pressure. Experiments can be run in both static and flow-through mode. The incubation system described here is usable for versatile purposes, not only the incubation of microorganisms and determination of growth rates, but also for chemical degradation or extraction experiments under high gas saturation, e.g., fluid-gas-rock-interactions in relation to carbon dioxide sequestration. As an application of the system we extracted organic compounds from sub-bituminous coal using H2O as well as a H2O-CO2 mixture at elevated temperature (90 degrees C) and pressure (5 MPa). Subsamples were taken at different time points during the incubation and analyzed by ion chromatography. Furthermore we demonstrated the applicability of the system for studies of microbial activity, using samples from the Isis mud volcano. We could detect an increase in sulfate reduction rate upon the addition of methane to the sample. KW - high-pressure incubation system KW - gas partial pressure KW - sub-sampling KW - carbon dioxide KW - low molecular weight organic acids Y1 - 2012 U6 - https://doi.org/10.3389/fmicb.2012.00025 SN - 1664-302X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Kaplan, Aaron A1 - Harel, Moshe A1 - Kaplan-Levy, Ruth N. A1 - Hadas, Ora A1 - Sukenik, Assaf A1 - Dittmann-Thünemann, Elke T1 - The languages spoken in the water body (or the biological role of cyanobacterial toxins) JF - Frontiers in microbiology N2 - Although intensification of toxic cyanobacterial blooms over the last decade is a matter of growing concern due to bloom impact on water quality, the biological role of most of the toxins produced is not known. In this critical review we focus primarily on the biological role of two toxins, microcystins and cylindrospermopsin, in inter- and intra-species communication and in nutrient acquisition. We examine the experimental evidence supporting some of the dogmas in the field and raise several open questions to be dealt with in future research. We do not discuss the health and environmental implications of toxin presence in the water body. KW - aoa KW - cylindrospermopsin KW - microcystin KW - cyr KW - mcy Y1 - 2012 U6 - https://doi.org/10.3389/fmicb.2012.00138 SN - 1664-302X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Bickel, Samantha L. A1 - Tang, Kam W. A1 - Grossart, Hans-Peter T1 - Ciliate epibionts associated with crustacean zooplankton in German lakes - distribution, motility, and bacterivory JF - Frontiers in microbiology N2 - Ciliate epibionts associated with crustacean zooplankton are widespread in aquatic systems, but their ecological roles are little known. We studied the occurrence of ciliate epibionts on crustacean zooplankton in nine German lakes with different limnological features during the summer of 2011. We also measured the detachment and re-attachment rates of the ciliates, changes in their motility, and the feeding rates of attached vs. detached ciliate epibionts. Epibionts were found in all lakes sampled except an acidic lake with large humic inputs. Epibiont prevalence was as high as 80.96% on the cladoceran Daphnia cucullata, 67.17% on the cladoceran Diaphanosoma brachyurum, and 46.67% on the calanoid copepod Eudiaptomus gracilis. Both cladoceran groups typically had less than 10 epibionts per individual, while the epibiont load on E. gracilis ranged from 1 to >30 epibionts per individual. After the death of the zooplankton host, the peritrich ciliate epibiont Epistylis sp. detached in an exponential fashion with a half-life of 5 min, and 98% detached within 30 min, leaving behind the stalks used for attachment. Immediately after detachment, the ciliates were immotile, but 62% became motile within 60 min. When a new host was present, only 27% reattached after 120 min. The average measured ingestion rate and clearance rate of Epistylis were 11,745 bacteria ciliate(-1) h(-1) and 24.33 mu l ciliate(-1) h(-1), respectively. Despite their high feeding rates, relatively low epibiont abundances were observed in the field, which suggests either diversion of energy to stalk formation, high metabolic loss by the epibionts, or high mortality among the epibiont populations. KW - ciliate epibionts KW - Epistylis KW - crustacean zooplankton KW - bacterivory KW - epibiont motility Y1 - 2012 U6 - https://doi.org/10.3389/fmicb.2012.00243 SN - 1664-302X VL - 3 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Mengel, Matthias A1 - Levermann, Anders A1 - Schleussner, Carl-Friedrich A1 - Born, Andreas T1 - Enhanced Atlantic subpolar gyre variability through baroclinic threshold in a coarse resolution model JF - Earth system dynamics N2 - Direct observations, satellite measurements and paleo records reveal strong variability in the Atlantic subpolar gyre on various time scales. Here we show that variations of comparable amplitude can only be simulated in a coupled climate model in the proximity of a dynamical threshold. The threshold and the associated dynamic response is due to a positive feedback involving increased salt transport in the subpolar gyre and enhanced deep convection in its centre. A series of sensitivity experiments is performed with a coarse resolution ocean general circulation model coupled to a statistical-dynamical atmosphere model which in itself does not produce atmospheric variability. To simulate the impact of atmospheric variability, the model system is perturbed with freshwater forcing of varying, but small amplitude and multi-decadal to centennial periodicities and observational variations in wind stress. While both freshwater and wind-stress-forcing have a small direct effect on the strength of the subpolar gyre, the magnitude of the gyre's response is strongly increased in the vicinity of the threshold. Our results indicate that baroclinic self-amplification in the North Atlantic ocean can play an important role in presently observed SPG variability and thereby North Atlantic climate variability on multi-decadal scales. Y1 - 2012 U6 - https://doi.org/10.5194/esd-3-189-2012 SN - 2190-4979 SN - 2190-4987 VL - 3 IS - 2 SP - 189 EP - 197 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Lauterbach, Daniel A1 - Ristow, Michael A1 - Gemeinholzer, Birgit T1 - Population genetics and fitness in fragmented populations of the dioecious and endangered Silene otites (Caryophyllaceae) JF - Plant systematics and evolution N2 - Population fragmentation is often correlated with loss of genetic diversity and reduced fitness. Obligate out-crossing (dioecy) is expected to enhance genetic diversity, reduce genetic differentiation, and avoid inbreeding depression through frequent gene flow. However, in highly fragmented populations dioecy has only diminishing effects upon genetic structure as pollination limitations (e.g. flight distance of pollinators) most often restrict inter-population gene flow in insect pollinated species. In fragmented dry grasslands in northeastern Germany, we analysed genetic structure, fitness, and habitat quality of the endangered dioecious Silene otites (Caryophyllaceae). Using AFLP markers, a high level of differentiation among ten populations was found (F (st) = 0.36), while the intra-population genetic diversities (H (E) = 0.165-0.240) were similar as compared to hermaphroditic species. There was neither a correlation between geographic and genetic distance nor between genetic diversity and population size, which indicates reduced gene flow among populations and random genetic drift. Plant size was positively correlated with genetic diversity. Seed set and number of juveniles were positively related to population size. Higher total coverage resulted in reduced plant fitness, and the number of juveniles was negatively correlated to cryptogam cover. Additionally, we found a sex ratio bias towards more male plants in larger populations. Overall, our results indicate that on a regional geographic scale dioecy does not necessarily prevent genetic erosion in the case of habitat fragmentation, especially in the absence of long distance seed and pollen dispersal capacity. KW - AFLP KW - Population size KW - Mating system KW - Isolation by distance KW - Sex ratio Y1 - 2012 U6 - https://doi.org/10.1007/s00606-011-0533-0 SN - 0378-2697 VL - 298 IS - 1 SP - 155 EP - 164 PB - Springer CY - Wien ER - TY - JOUR A1 - Araujo, Wagner L. A1 - Nunes-Nesi, Adriano A1 - Nikoloski, Zoran A1 - Sweetlove, Lee J. A1 - Fernie, Alisdair R. T1 - Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues JF - Plant, cell & environment : cell physiology, whole-plant physiology, community physiology N2 - The tricarboxylic acid (TCA) cycle is a crucial component of respiratory metabolism in both photosynthetic and heterotrophic plant organs. All of the major genes of the tomato TCA cycle have been cloned recently, allowing the generation of a suite of transgenic plants in which the majority of the enzymes in the pathway are progressively decreased. Investigations of these plants have provided an almost complete view of the distribution of control in this important pathway. Our studies suggest that citrate synthase, aconitase, isocitrate dehydrogenase, succinyl CoA ligase, succinate dehydrogenase, fumarase and malate dehydrogenase have control coefficients flux for respiration of -0.4, 0.964, -0.123, 0.0008, 0.289, 0.601 and 1.76, respectively; while 2-oxoglutarate dehydrogenase is estimated to have a control coefficient of 0.786 in potato tubers. These results thus indicate that the control of this pathway is distributed among malate dehydrogenase, aconitase, fumarase, succinate dehydrogenase and 2-oxoglutarate dehydrogenase. The unusual distribution of control estimated here is consistent with specific non-cyclic flux mode and cytosolic bypasses that operate in illuminated leaves. These observations are discussed in the context of known regulatory properties of the enzymes and some illustrative examples of how the pathway responds to environmental change are given. KW - metabolic control analysis KW - metabolic regulation KW - respiration KW - Solanum lycopersicum (tomato) KW - TCA cycle Y1 - 2012 U6 - https://doi.org/10.1111/j.1365-3040.2011.02332.x SN - 0140-7791 VL - 35 IS - 1 SP - 1 EP - 21 PB - Wiley-Blackwell CY - Hoboken ER - TY - JOUR A1 - Stojanovic, Milovan A1 - Markovic, Rade A1 - Kleinpeter, Erich A1 - Baranac-Stojanovic, Marija T1 - Synthesis of thiazolidine-fused heterocycles via exo-mode cyclizations of vinylogous N-acyliminium ions JF - Organic & biomolecular chemistry : an international journal of synthetic, physical and biomolecular organic chemistry N2 - Syntheses of thiazolidine-fused heterocycles via exo-mode cyclizations of vinylogous N-acyliminium ions incorporating heteroatom-based nucleophiles have been examined and discussed. The formation of (5,6)-membered systems was feasible with all nucleophiles tried (O, S and N), while the closing of the five-membered ring was restricted to O- and S-nucleophiles. The closure of a four-membered ring failed. Instead, the bicyclic (5,6)-membered acetal derivative and the tricyclic system with an eight-membered central ring were obtained from the substrates containing O and S nucleophilic moieties, respectively. The reaction outcome and stereochemistry are rationalized using quantum chemical calculations at B3LYP/6-31G(d) level. The exclusive cis-stereoselectivity in the formation of (5,6)- and (5,5)-membered systems results from thermodynamic control, whereas the formation of the eight-membered ring was kinetically controlled. Y1 - 2012 U6 - https://doi.org/10.1039/c1ob06451g SN - 1477-0520 VL - 10 IS - 3 SP - 575 EP - 589 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Graae, Bente J. A1 - De Frenne, Pieter A1 - Kolb, Annette A1 - Brunet, Jorg A1 - Chabrerie, Olivier A1 - Verheyen, Kris A1 - Pepin, Nick A1 - Heinken, Thilo A1 - Zobel, Martin A1 - Shevtsova, Anna A1 - Nijs, Ivan A1 - Milbau, Ann T1 - On the use of weather data in ecological studies along altitudinal and latitudinal gradients JF - Oikos N2 - Global warming has created a need for studies along climatic gradients to assess the effects of temperature on ecological processes. Altitudinal and latitudinal gradients are often used as such, usually in combination with air temperature data from the closest weather station recorded at 1.52 m above the ground. However, many ecological processes occur in, at, or right above the soil surface. To evaluate how representative the commonly used weather station data are for the microclimate relevant for soil surface biota, we compared weather station temperatures for an altitudinal (500900 m a.s.l.) and a latitudinal gradient (4968 degrees N) with data obtained by temperature sensors placed right below the soil surface at five sites along these gradients. The mean annual temperatures obtained from weather stations and adjusted using a lapse rate of -5.5 degrees C km-1 were between 3.8 degrees C lower and 1.6 degrees C higher than those recorded by the temperature sensors at the soil surface, depending on the position along the gradients. The monthly mean temperatures were up to 10 degrees C warmer or 5 degrees C colder at the soil surface. The within-site variation in accumulated temperature was as high as would be expected from a 300 m change in altitude or from a 4 degrees change in latitude or a climate change scenario corresponding to warming of 1.63.8 degrees C. Thus, these differences introduced by the decoupling are significant from a climate change perspective, and the results demonstrate the need for incorporating microclimatic variation when conducting studies along altitudinal or latitudinal gradients. We emphasize the need for using relevant temperature data in climate impact studies and further call for more studies describing the soil surface microclimate, which is crucial for much of the biota. Y1 - 2012 U6 - https://doi.org/10.1111/j.1600-0706.2011.19694.x SN - 0030-1299 VL - 121 IS - 1 SP - 3 EP - 19 PB - Wiley-Blackwell CY - Hoboken ER - TY - INPR A1 - Baret, Jean-Christophe A1 - Belder, Detlev A1 - Bier, Frank Fabian A1 - Cao, Jialan A1 - Gruschke, Oliver A1 - Hardt, Steffen A1 - Kirschbaum, Michael A1 - Koehler, J. Michael A1 - Schumacher, Soeren A1 - Urban, G. A. A1 - Viefhues, Martina T1 - Contributors to the 10th Anniversary Germany issue T2 - LAB on a chip : miniaturisation for chemistry and biology Y1 - 2012 U6 - https://doi.org/10.1039/c1lc90139g SN - 1473-0197 VL - 12 IS - 3 SP - 419 EP - 421 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Wojcinski, Sebastian A1 - Cassel, Michael A1 - Farrokh, Andre A1 - Soliman, Amr A. A1 - Hille, Ursula A1 - Schmidt, Werner A1 - Degenhardt, Friedrich A1 - Hillemanns, Peter T1 - Variations in the elasticity of breast tissue during the menstrual cycle determined by real-time sonoelastography JF - Journal of ultrasound in medicine N2 - Objectives-The purpose of this study was to determine the dependence of breast tissue elasticity on the menstrual cycle of healthy volunteers by means of real-time sonoelastography. Methods-Twenty-two healthy volunteers (aged 18-33 years) were examined once weekly during two consecutive menstrual cycles using sonoelastography. Group 1 (n = 10) was not taking hormonal medication; group 2 (n = 12) was taking oral contraceptives. Results-The breast parenchyma appeared softer than the dermis and harder than the adipose tissue, and elasticity varied over the menstrual cycle and between groups. Group 1 (no hormone intake) showed continuously increasing elasticity with relatively soft breast parenchyma in the menstrual and follicular phases and harder parenchyma in the luteal phase (P = .012). Group 2 (oral contraceptives) showed no statistically significant changes in breast parenchymal elasticity according to sonoelastography. The parenchyma was generally softer in group 1 compared with group 2 throughout the menstrual cycle (P = .033). The dermis, the subcutaneous adipose tissue, and the pectoralis major muscle showed no changes in elasticity. Comparison of measurements made during the first and the second menstrual cycles showed similar patterns of elasticity in both groups. Conclusions-Sonoelastography is a reproducible method that can be used to determine the dependence of breast parenchyma elasticity on the menstrual cycle and on the intake of hormonal contraceptives. KW - breast tissue KW - elasticity imaging KW - real-time tissue elastography KW - sonoelastography Y1 - 2012 SN - 0278-4297 SN - 1550-9613 VL - 31 IS - 1 SP - 63 EP - 72 PB - American Institute of Ultrasound in Medicine CY - Laurel ER - TY - JOUR A1 - Rudolph, Nicole A1 - Esser, Hanna G. A1 - Carminati, Andrea A1 - Moradi, Ahmad B. A1 - Hilger, Andre A1 - Kardjilov, Nikolay A1 - Nagl, Stefan A1 - Oswald, Sascha T1 - Dynamic oxygen mapping in the root zone by fluorescence dye imaging combined with neutron radiography JF - Journal of soils and sediments : protection, risk assessment and remediation N2 - The rooted zone of a soil, more precisely the rhizosphere, is a very dynamic system. Some of the key processes are water uptake and root respiration. We have developed a novel method for measuring the real-time distribution of water and oxygen concentration in the rhizosphere as a biogeochemical interface in soil. This enables understanding where and when roots are active in respect to root respiration and water uptake and how the soil responds to it. We used glass containers (15 x 15 x 1 cm), which were filled with a quartz sand mixture. Sensor foils for fluorescence dye imaging of O-2 were installed on the inner side of the containers. A lupine plant was grown in each container for 2 weeks under controlled conditions. Then we took time series of fluorescence images for time-lapsed visualization of oxygen depletion caused by root respiration. Changing water content was mapped in parallel by non-invasive neutron radiography, which yields water content distributions in high spatial resolution. Also it can detect the root system of the lupine plants. By this combined imaging of the samples, a range of water contents and different oxygen concentration levels, both induced by root activities, could be assessed. We monitored the dynamics of these vital parameters induced by roots during a period of several hours. We observed that for high water saturation, the oxygen concentration decreased in parts of the container. The accompanying neutron radiographies gave us the information that these locations are spatially correlated to roots. Therefore, it can be concluded that the observed oxygen deficits close to the roots result from root respiration and show up while re-aeration from atmosphere by gas phase transport is restricted by the high water saturation. Our coupled imaging setup was able to monitor the spatial distribution and temporal dynamics of oxygen and water content in a night and day cycle. This reflects complex plant activities such as photosynthesis, transpiration, and metabolic activities impacting the root-soil interface. Our experimental setup provides the possibility to non-invasively visualize these parameters with high resolution. The particular oxygen imaging method as well as the combination with simultaneously mapping the water content by neutron radiography is a novelty. KW - Fluorescence imaging KW - Neutron radiography KW - Oxygen mapping KW - Rhizosphere KW - Root respiration KW - Water distribution Y1 - 2012 U6 - https://doi.org/10.1007/s11368-011-0407-7 SN - 1439-0108 VL - 12 IS - 1 SP - 63 EP - 74 PB - Springer CY - Heidelberg ER - TY - JOUR A1 - Herfurth, Christoph A1 - Voll, Dominik A1 - Buller, Jens A1 - Weiss, Jan A1 - Barner-Kowollik, Christopher A1 - Laschewsky, André T1 - Radical addition fragmentation chain transfer (RAFT) polymerization of ferrocenyl (meth)acrylates JF - Journal of polymer science : A, Polymer chemistry N2 - We report on the controlled free radical homopolymerization of 1-ferrocenylethyl acrylate as well as of three new ferrocene bearing monomers, namely 4-ferrocenylbutyl acrylate, 2-ferrocenylamido-2-methylpropyl acrylate, and 4-ferrocenylbutyl methacrylate, by the RAFT technique. For comparison, the latter monomer was polymerized using ATRP, too. The ferrocene containing monomers were found to be less reactive than their analogues free of ferrocene. The reasons for the low polymerizability are not entirely clear. As the addition of free ferrocene to the reaction mixture did not notably affect the polymerizations, sterical hindrance by the bulky ferrocene moiety fixed on the monomers seems to be the most probable explanation. Molar masses found for 1-ferrocenylethyl acrylate did not exceed 10,000 g mol(-1), while for 4-ferrocenylbutyl (meth) acrylate molar masses of 15,000 g mol(-1) could be obtained. With PDIs as low as 1.3 in RAFT polymerization of the monomers, good control over the polymerization was achieved. KW - ferrocene KW - living radical polymerization (LRP) KW - monomers KW - radical addition fragmentation chain transfer (RAFT) KW - radical polymerization KW - redox polymers KW - synthesis Y1 - 2012 U6 - https://doi.org/10.1002/pola.24994 SN - 0887-624X VL - 50 IS - 1 SP - 108 EP - 118 PB - Wiley-Blackwell CY - Malden ER -