TY - GEN A1 - Blenau, Wolfgang A1 - Rotte, Cathleen A1 - Krach, Christian A1 - Balfanz, Sabine A1 - Baumann, Arnd A1 - Walz, Bernd T1 - Molecular characterization and localization of the first tyramine receptor of the American cockroach (Periplaneta americana) N2 - The phenolamines octopamine and tyramine control, regulate, and modulate many physiological and behavioral processes in invertebrates. Vertebrates possess only small amounts of both substances, and thus, octopamine and tyramine, together with other biogenic amines, are referred to as “trace amines.” Biogenic amines evoke cellular responses by activating G-protein-coupled receptors. We have isolated a complementary DNA (cDNA) that encodes a biogenic amine receptor from the American cockroach Periplaneta americana, viz., Peatyr1, which shares high sequence similarity to members of the invertebrate tyramine-receptor family. The PeaTYR1 receptor was stably expressed in human embryonic kidney (HEK) 293 cells, and its ligand response has been examined. Receptor activation with tyramine reduces adenylyl cyclase activity in a dose-dependent manner (EC50 350 nM). The inhibitory effect of tyramine is abolished by co-incubation with either yohimbine or chlorpromazine. Receptor expression has been investigated by reverse transcription polymerase chain reaction and immunocytochemistry. The mRNA is present in various tissues including brain, salivary glands, midgut, Malpighian tubules, and leg muscles. The effect of tyramine on salivary gland acinar cells has been investigated by intracellular recordings, which have revealed excitatory presynaptic actions of tyramine. This study marks the first comprehensive molecular, pharmacological, and functional characterization of a tyramine receptor in the cockroach. KW - Biogenic amine KW - cellular signaling KW - G-protein-coupled receptor KW - octopamine KW - salivary gland Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44335 ER - TY - GEN A1 - Wehinger, Brunhilde T1 - Mode, Kleidung, Kunst : Rezensionsessay N2 - rezensierte Werke: Bachmann, Cordula: Kleidung und Geschlecht : Ethnographische Erkundungen einer Alltagspraxis / Cordula Bachmann. - Bielefeld : transcript Verlag, 2008. - 154 S. ISBN 978-3-89942-920-6 Pape, Cora von: Kunstkleider : die Präsenz des Körpers in textilen Kunst-Objekten des 20. Jahrhunderts / Cora von Pape. - Bielefeld : transcript Verlag, 2008. - 225 S. ISBN 978-3-89942-825-4 Zwei neue Studien widmen sich dem Verhältnis von Kleidung und Geschlecht beziehungsweise Kleidung und Körper. Wie Frauen und Männer in ihrer alltäglichen Praxis des ‚Sich-Kleidens‘ mit den modischen Erscheinungsbildern von Männlichkeit oder Weiblichkeit umgehen, untersucht die Kulturwissenschaftlerin Cordula Bachmann. Sie wertet aktuelle Interviews zum Kleidungsverhalten von Frauen und Männern aus und rückt dabei die Perspektive der Handelnden, die das alltägliche ‚Sich-Kleiden‘ als eine primäre soziale Anforderung zu lösen haben, ins Zentrum des Interesses. Den aufsehenerregenden künstlerischen Umgang mit Kleidung in Form von textilen Kunstobjekten, die sich mit dem menschlichen Körper befassen und der Art und Weise, wie er mittels Kleidung und Textilien repräsentiert oder transformiert wird, untersucht die Kunsthistorikerin Cora von Pape. Das alltägliche weibliche Chaos des Sich-Ankleidens angesichts der täglichen Herausforderung, sich bei der Entscheidung für oder gegen ein Kleid, sich auch für oder gegen eine Frauenrolle entscheiden zu müssen, das in den von Bachmann ausgewerteten Interviews thematisiert wird, scheint auch in den Performances oder textilen Kunstobjekten vieler von Cordula von Pape vorgestellten Künstlerinnen der 2. Hälfte des 20. Jahrhunderts auf, allerdings künstlerisch-experimentell bearbeitet in eindrucksvollen (Selbst-)Inszenierungen, die der gewaltigen Symbolik des Sich-Ankleidens, der Kleider als Körperhüllen oder Reliquien, die an die Stelle des verschwundenen Körpers treten, auf den Grund gehen. T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 77 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45464 ER - TY - GEN A1 - Koseska, Aneta A1 - Zaikin, Alexey A1 - Kurths, Jürgen A1 - García-Ojalvo, Jordi T1 - Timing cellular decision making under noise via cell-cell communication N2 - Many cellular processes require decision making mechanisms, which must act reliably even in the unavoidable presence of substantial amounts of noise. However, the multistable genetic switches that underlie most decision-making processes are dominated by fluctuations that can induce random jumps between alternative cellular states. Here we show, via theoretical modeling of a population of noise-driven bistable genetic switches, that reliable timing of decision-making processes can be accomplished for large enough population sizes, as long as cells are globally coupled by chemical means. In the light of these results, we conjecture that cell proliferation, in the presence of cell-cell communication, could provide a mechanism for reliable decision making in the presence of noise, by triggering cellular transitions only when the whole cell population reaches a certain size. In other words , the summation performed by the cell population would average out the noise and reduce its detrimental impact. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 148 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45260 ER - TY - GEN A1 - Szymanski, Jedrzej A1 - Jozefczuk, Szymon A1 - Nikoloski, Zoran A1 - Selbig, Joachim A1 - Nikiforova, Victoria A1 - Catchpole, Gareth A1 - Willmitzer, Lothar T1 - Stability of metabolic correlations under changing environmental conditions in Escherichia coli : a systems approach N2 - Background: Biological systems adapt to changing environments by reorganizing their cellula r and physiological program with metabolites representing one important response level. Different stresses lead to both conserved and specific responses on the metabolite level which should be reflected in the underl ying metabolic network. Methodology/Principal Findings: Starting from experimental data obtained by a GC-MS based high-throughput metabolic profiling technology we here develop an approach that: (1) extracts network representations from metabolic conditiondependent data by using pairwise correlations, (2) determines the sets of stable and condition-dependent correlations based on a combination of statistical significance and homogeneity tests, and (3) can identify metabolites related to the stress response, which goes beyond simple ob servation s about the changes of metabolic concentrations. The approach was tested with Escherichia colias a model organism observed under four different environmental stress conditions (cold stress, heat stress, oxidative stress, lactose diau xie) and control unperturbed conditions. By constructing the stable network component, which displays a scale free topology and small-world characteristics, we demonstrated that: (1) metabolite hubs in this reconstructed correlation networks are significantly enriched for those contained in biochemical networks such as EcoCyc, (2) particular components of the stable network are enriched for functionally related biochemical path ways, and (3) ind ependently of the response scale, based on their importance in the reorganization of the cor relation network a set of metabolites can be identified which represent hypothetical candidates for adjusting to a stress-specific response. Conclusions/Significance: Network-based tools allowed the identification of stress-dependent and general metabolic correlation networks. This correlation-network-ba sed approach does not rely on major changes in concentration to identify metabolites important for st ress adaptation, but rather on the changes in network properties with respect to metabolites. This should represent a useful complementary technique in addition to more classical approaches. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 147 KW - Small-world networks KW - saccharomyces-cerevisiae KW - trehalose synthesis KW - gene-expression KW - stress-response Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45253 ER - TY - GEN A1 - Wienhöfer, Jan A1 - Germer, Kai A1 - Lindenmaier, Falk A1 - Färber, Arne A1 - Zehe, Erwin T1 - Applied tracers for the observation of subsurface stormflow at the hillslope scale N2 - Rain fall-runoff response in temperate humid headwater catchments is mainly controlled by hydrolo gical processes at the hillslope scale. Applied tracer experiments with fluore scent dye and salt tracers are well known tools in groundwater studies at the large scale and vadose zone studies at the plot scale, where they provide a means to characterise subsurface flow. We extend this approach to the hillslope scale to investigate saturated and unsaturated flow path s concertedly at a forested hill slope in the Austrian Alps. Dye staining experiments at the plot scale revealed that crack s and soil pipe s function as preferential flow path s in the fine-textured soils of the study area, and these preferenti al flow structures were active in fast subsurface transport of tracers at the hillslope scale. Breakthrough curves obtained under steady flow conditions could be fitted well to a one-dimensional convection-dispersion model. Under natural rain fall a positive correlation of tracer concentrations to the transient flows was observed. The results of this study demon strate qualitative and quantitative effects of preferential flow feature s on subsurface stormflow in a temperate humid headwater catchment. It turn s out that , at the hill slope scale, the interaction s of structures and processes are intrinsically complex, which implies that attempts to model such a hillslope satisfactorily require detailed investigation s of effective structures and parameters at the scale of interest. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 146 KW - Saturated hydraulic conductivity KW - preferential flow pathways KW - solute transport KW - runoff generation KW - fluorescent dyes Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45246 ER - TY - GEN A1 - Mell, Thomas A1 - Wartenburger, Isabell A1 - Marschner, Alexander A1 - Villringer, Arno A1 - Reischies, Friedel M. A1 - Heekeren, Hauke R. T1 - Altered function of ventral striatum during reward-based decision making in old age N2 - Normal aging is associated with a decline in different cognitive domains and local structural atrophy as well as decreases in dopamine concentration and receptor density. To date, it is largely unknown how these reductions in dopaminergic neurotransmission affect human brain regions responsible for reward-based decision making in older adults. Using a learning criterion in a probabilistic object reversal task, we found a learning stage by age interaction in the dorsolateral prefrontal cortex (dIPFC) during decision making. While young adults recruited the dlPFC in an early stage of learning reward associations, older adults recruited the dlPFC when reward associations had already been learned. Furthermore, we found a reduced change in ventral striatal BOLD signal in older as compared to younger adults in response to high probability rewards. Our data are in line with behavioral evidence that older adults show altered stimulus-reward learning and support the view of an altered fronto-striatal interaction during reward-based decision making in old age, which contributes to prolonged learning of reward associations. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - paper 182 KW - aging KW - fMRI KW - reward association learning KW - ventral striatum KW - decision making KW - dorsolateral prefrontal cortex Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45235 ER - TY - GEN A1 - Childs, Liam H. A1 - Nikoloski, Zoran A1 - May, Patrick A1 - Walther, Dirk T1 - Identification and classification of ncRNA molecules using graph properties N2 - The study of non-coding RNA genes has received increased attention in recent years fuelled by accumulating evidence that larger portions of genomes than previously acknowledged are transcribed into RNA molecules of mostly unknown function, as well as the discovery of novel non-coding RNA types and functional RNA elements. Here, we demonstrate that specific properties of graphs that represent the predicted RNA secondary structure reflect functional information. We introduce a computational algorithm and an associated web-based tool (GraPPLE) for classifying non-coding RNA molecules as functional and, furthermore, into Rfam families based on their graph properties. Unlike sequence-similarity-based methods and covariance models, GraPPLE is demonstrated to be more robust with regard to increasing sequence divergence, and when combined with existing methods, leads to a significant improvement of prediction accuracy. Furthermore, graph properties identified as most informative are shown to provide an understanding as to what particular structural features render RNA molecules functional. Thus, GraPPLE may offer a valuable computational filtering tool to identify potentially interesting RNA molecules among large candidate datasets. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 145 KW - RNA secondary structure KW - Noncoding RNAs KW - Structure prediction KW - Gene-expression KW - Structured RNAs Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45192 ER - TY - GEN A1 - Cox, Tom A1 - Maris, Tom A1 - Soetart, Karline A1 - Conley, Daniel A1 - van Damme, Stefan A1 - Meire, Patrick A1 - Middelburg, Jack J. A1 - Vos, Matthijs A1 - Struyf, Eric T1 - A macro-tidal freshwater ecosystem recovering from hypereutrophication : the Schelde lease study N2 - We report a 40 year record of eutrophication and hypoxia on an estuarine ecosystem and its recovery from hypereutrophication. After decades of high inorganic nutrient concentrations and recurring anoxia and hypoxia, we observe a paradoxical increase in chlorophyll-a concentrations with decreasing nutrient inputs. We hypothesise that algal growth was inhibited due to hypereutrophication, either by elevated ammonium concentrations, severe hypoxia or the production of harmful substances in such a reduced environment. We study the dynamics of a simple but realistic mathematical model, incorporating the assumption of algal growth inhibition. It shows a high algal biomass, net oxygen production equilibrium with low ammonia inputs, and a low algal biomass, net oxygen consumption equilibrium with high ammonia inputs. At intermediate ammonia inputs it displays two alternative stable states. Although not intentional, the numerical output of this model corresponds to observations, giving extra support for assumption of algal growth inhibition. Due to potential algal growth inhibition, the recovery of hypereutrophied systems towards a classical eutrophied state, will need reduction of waste loads below certain thresholds and will be accompanied by large fluctuations in oxygen concentrations. We conclude that also flow-through systems, heavily influenced by external forcings which partly mask internal system dynamics, can display multiple stable states. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 144 KW - Estuary SW Netherlands KW - southern North-Sea KW - Past 50 years KW - Westerschelde estuary KW - Marine ecosystems Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45180 ER - TY - GEN A1 - Ziege, Madlen A1 - Mahlow, Kristin A1 - Hennige-Sulz, Carmen A1 - Kronmarck, Claudia A1 - Tiedemann, Ralph A1 - Streit, Bruno A1 - Plath, Martin T1 - Audience effects in the Atlantic molly (Poecilia mexicana) : prudent male mate choice in response to perceived sperm competition risk? N2 - Background: Multidirectional interactions in social networks can have a profound effect on mate choice behavior; e.g., Poecilia mexicana males show weaker expression of mating preferences when being observed by a rival. This may be an adaptation to reduce sperm competition risk, which arises because commonly preferred female phenotypes will receive attention also from surrounding males, and/or because other males can copy the focal male's mate choice. Do P. mexicana males indeed respond to perceived sperm competition risk? We gave males a choice between two females and repeated the tests under one of the following conditions: (1) an empty transparent cylinder was presented (control); (2) another ("audience") male inside the cylinder observed the focal male throughout the 2nd part, or (3) the audience male was presented only before the tests, but could not eavesdrop during the actual choice tests (non-specific sperm competition risk treatments); (4) the focal male could see a rival male interact sexually with the previously preferred, or (5) with the non-preferred female before the 2nd part of the tests (specific sperm competition risk treatments). Results: The strength of individual male preferences declined slightly also during the control treatment (1). However, this decrease was more than two-fold stronger in audience treatment (2), i.e., with non-specific sperm competition risk including the possibility for visual eavesdropping by the audience male. No audience effect was found in treatments (3) and (5), but a weak effect was also observed when the focal male had seen the previously preferred female sexually interact with a rival male (treatment 4; specific sperm competition risk). Conclusion: When comparing the two 'non-specific sperm competition risk' treatments, a very strong effect was found only when the audience male could actually observe the focal male during mate choice [treatment (2)]. This suggests that focal males indeed attempt to conceal their mating preferences so as to prevent surrounding males from copying their mate choice. When there is no potential for eavesdropping [treatment (3)], non-specific specific sperm competition risk seems to play a minor or no role. Our results also show that P. mexicana males tend to share their mating effort more equally among females when the resource value of their previously preferred mate decreases after mating with a rival male (perceived specific sperm competition risk), but this effect is comparatively weak. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 143 KW - Siamese fighting fish KW - Guppies poecilia-reticulata KW - Betta-splendens KW - Public information KW - Mating preferences Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45171 ER - TY - GEN A1 - Gärtner, Tanja A1 - Steinfath, Matthias A1 - Andorf, Sandra A1 - Lisec, Jan A1 - Meyer, Rhonda C. A1 - Altmann, Thomas A1 - Willmitzer, Lothar A1 - Selbig, Joachim T1 - Improved heterosis prediction by combining information on DNA- and metabolic markers N2 - Background: Hybrids represent a cornerstone in the success story of breeding programs. The fundamental principle underlying this success is the phenomenon of hybrid vigour, or heterosis. It describes an advantage of the offspring as compared to the two parental lines with respect to parameters such as growth and resistance against abiotic or biotic stress. Dominance, overdominance or epistasis based models are commonly used explanations. Conclusion/Significance: The heterosis level is clearly a function of the combination of the parents used for offspring production. This results in a major challenge for plant breeders, as usually several thousand combinations of parents have to be tested for identifying the best combinations. Thus, any approach to reliably predict heterosis levels based on properties of the parental lines would be highly beneficial for plant breeding. Methodology/Principal Findings: Recently, genetic data have been used to predict heterosis. Here we show that a combination of parental genetic and metabolic markers, identified via feature selection and minimum-description-length based regression methods, significantly improves the prediction of biomass heterosis in resulting offspring. These findings will help furthering our understanding of the molecular basis of heterosis, revealing, for instance, the presence of nonlinear genotype-phenotype relationships. In addition, we describe a possible approach for accelerated selection in plant breeding. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 142 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45132 ER - TY - GEN A1 - Durek, Pawel A1 - Schudoma, Christian A1 - Weckwerth, Wolfram A1 - Selbig, Joachim A1 - Walther, Dirk T1 - Detection and characterization of 3D-signature phosphorylation site motifs and their contribution towards improved phosphorylation site prediction in proteins N2 - Background: Phosphorylation of proteins plays a crucial role in the regulation and activation of metabolic and signaling pathways and constitutes an important target for pharmaceutical intervention. Central to the phosphorylation process is the recognition of specific target sites by protein kinases followed by the covalent attachment of phosphate groups to the amino acids serine, threonine, or tyrosine. The experimental identification as well as computational prediction of phosphorylation sites (P-sites) has proved to be a challenging problem. Computational methods have focused primarily on extracting predictive features from the local, one-dimensional sequence information surrounding phosphorylation sites. Results: We characterized the spatial context of phosphorylation sites and assessed its usability for improved phosphorylation site predictions. We identified 750 non-redundant, experimentally verified sites with three-dimensional (3D) structural information available in the protein data bank (PDB) and grouped them according to their respective kinase family. We studied the spatial distribution of amino acids around phosphorserines, phosphothreonines, and phosphotyrosines to extract signature 3D-profiles. Characteristic spatial distributions of amino acid residue types around phosphorylation sites were indeed discernable, especially when kinase-family-specific target sites were analyzed. To test the added value of using spatial information for the computational prediction of phosphorylation sites, Support Vector Machines were applied using both sequence as well as structural information. When compared to sequence-only based prediction methods, a small but consistent performance improvement was obtained when the prediction was informed by 3D-context information. Conclusion: While local one-dimensional amino acid sequence information was observed to harbor most of the discriminatory power, spatial context information was identified as relevant for the recognition of kinases and their cognate target sites and can be used for an improved prediction of phosphorylation sites. A web-based service (Phos3D) implementing the developed structurebased P-site prediction method has been made available at http://phos3d.mpimp-golm.mpg.de. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 141 KW - Support vector machines KW - Microarray data KW - Docking interactions KW - Signal-transduction KW - Sequence alignment Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45129 ER - TY - GEN A1 - Reusser, Dominik A1 - Blume, Theresa A1 - Schaefli, Bettina A1 - Zehe, Erwin T1 - Analysing the temporal dynamics of model performance for hydrological models N2 - The temporal dynamics of hydrological model performance gives insights into errors that cannot be obtained from global performance measures assigning a single number to the fit of a simulated time series to an observed reference series. These errors can include errors in data, model parameters, or model structure. Dealing with a set of performance measures evaluated at a high temporal resolution implies analyzing and interpreting a high dimensional data set. This paper presents a method for such a hydrological model performance assessment with a high temporal resolution and illustrates its application for two very different rainfall-runoff modeling case studies. The first is the Wilde Weisseritz case study, a headwater catchment in the eastern Ore Mountains, simulated with the conceptual model WaSiM-ETH. The second is the Malalcahuello case study, a headwater catchment in the Chilean Andes, simulated with the physicsbased model Catflow. The proposed time-resolved performance assessment starts with the computation of a large set of classically used performance measures for a moving window. The key of the developed approach is a data-reduction method based on self-organizing maps (SOMs) and cluster analysis to classify the high-dimensional performance matrix. Synthetic peak errors are used to interpret the resulting error classes. The final outcome of the proposed method is a time series of the occurrence of dominant error types. For the two case studies analyzed here, 6 such error types have been identified. They show clear temporal patterns, which can lead to the identification of model structural errors. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 140 KW - Rainfall-runoff response KW - Process identification KW - Improved calibration KW - Soil-moisture KW - Catchment Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45114 ER - TY - GEN A1 - Schröder, Florian A1 - Lisso, Janina A1 - Lange, Peggy A1 - Müssig, Carsten T1 - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves N2 - Background: The EXO (EXORDIUM) gene was identified as a potential mediator of brassinosteroid (BR)-promoted growth. It is part of a gene family with eight members in Arabidopsis. EXO gene expression is under control of BR, and EXO overexpression promotes shoot and root growth. In this study, the consequences of loss of EXO function are described. Results: The exo loss of function mutant showed diminished leaf and root growth and reduced biomass production. Light and scanning electron microscopy analyses revealed that impaired leaf growth is due to reduced cell expansion. Epidermis, palisade, and spongy parenchyma cells were smaller in comparison to the wild-type. The exo mutant showed reduced brassinolide-induced cotyledon and hypocotyl growth. In contrast, exo roots were significantly more sensitive to the inhibitory effect of synthetic brassinolide. Apart from reduced growth, exo did not show severe morphological abnormalities. Gene expression analyses of leaf material identified genes that showed robust EXO-dependent expression. Growth-related genes such as WAK1, EXP5, and KCS1, and genes involved in primary and secondary metabolism showed weaker expression in exo than in wild-type plants. However, the vast majority of BR-regulated genes were normally expressed in exo. HA- and GFP-tagged EXO proteins were targeted to the apoplast. Conclusion: The EXO gene is essential for cell expansion in leaves. Gene expression patterns and growth assays suggest that EXO mediates BR-induced leaf growth. However, EXO does not control BR-levels or BR-sensitivity in the shoot. EXO presumably is involved in a signalling process which coordinates BR-responses with environmental or developmental signals. The hypersensitivity of exo roots to BR suggests that EXO plays a diverse role in the control of BR responses in the root. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 139 KW - Plant transformation KW - Gene expression KW - Wall proteins KW - Thaliana KW - Brassinosteroids Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45107 ER - TY - GEN A1 - Szarzynska, Bogna A1 - Sobkowiak, Lukasz A1 - Pant, Bikram Datt A1 - Balazadeh, Salma A1 - Scheible, Wolf-Rüdiger A1 - Müller-Röber, Bernd A1 - Jarmolowski, Artur A1 - Szweykowska-Kulinska, Zofia T1 - Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs N2 - Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced primiRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 138 KW - Binding-protein hyl1 KW - Abscisic-acid KW - Flowering time KW - Micro-RNA KW - Serrate Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45085 ER - TY - GEN A1 - Riano-Pachon, Diego Mauricio A1 - Nagel, Axel A1 - Neigenfind, Jost A1 - Wagner, Robert A1 - Basekow, Rico A1 - Weber, Elke A1 - Müller-Röber, Bernd A1 - Diehl, Svenja A1 - Kersten, Birgit T1 - GabiPD : the GABI primary database - a plant integrative "omics" database N2 - The GABI Primary Database, GabiPD (http:// www.gabipd.org/), was established in the frame of the German initiative for Genome Analysis of the Plant Biological System (GABI). The goal of GabiPD is to collect, integrate, analyze and visualize primary information from GABI projects. GabiPD constitutes a repository and analysis platform for a wide array of heterogeneous data from high-throughput experiments in several plant species. Data from different ‘omics’ fronts are incorporated (i.e. genomics, transcriptomics, proteomics and metabolomics), originating from 14 different model or crop species. We have developed the concept of GreenCards for textbased retrieval of all data types in GabiPD (e.g. clones, genes, mutant lines). All data types point to a central Gene GreenCard, where gene information is integrated from genome projects or NCBI UniGene sets. The centralized Gene GreenCard allows visualizing ESTs aligned to annotated transcripts as well as displaying identified protein domains and gene structure. Moreover, GabiPD makes available interactive genetic maps from potato and barley, and protein 2DE gels from Arabidopsis thaliana and Brassica napus. Gene expression and metabolic-profiling data can be visualized through MapManWeb. By the integration of complex data in a framework of existing knowledge, GabiPD provides new insights and allows for new interpretations of the data. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 137 KW - Phosphorylation sites KW - Arabidopsis thaliana KW - Information KW - Proteins KW - Families Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45075 ER - TY - GEN A1 - Lamprecht, Anna-Lena A1 - Margaria, Tiziana A1 - Steffen, Bernhard T1 - Bio-jETI : a framework for semantics-based service composition N2 - Background: The development of bioinformatics databases, algorithms, and tools throughout the last years has lead to a highly distributedworld of bioinformatics services. Without adequatemanagement and development support, in silico researchers are hardly able to exploit the potential of building complex, specialized analysis processes from these services. The Semantic Web aims at thoroughly equipping individual data and services with machine-processable meta-information, while workflow systems support the construction of service compositions. However, even in this combination, in silico researchers currently would have to deal manually with the service interfaces, the adequacy of the semantic annotations, type incompatibilities, and the consistency of service compositions. Results: In this paper, we demonstrate by means of two examples how Semantic Web technology together with an adequate domain modelling frees in silico researchers from dealing with interfaces, types, and inconsistencies. In Bio-jETI, bioinformatics services can be graphically combined to complex services without worrying about details of their interfaces or about type mismatches of the composition. These issues are taken care of at the semantic level by Bio-jETI’s model checking and synthesis features. Whenever possible, they automatically resolve type mismatches in the considered service setting. Otherwise, they graphically indicate impossible/incorrect service combinations. In the latter case, the workflow developermay either modify his service composition using semantically similar services, or ask for help in developing the missing mediator that correctly bridges the detected type gap. Newly developed mediators should then be adequately annotated semantically, and added to the service library for later reuse in similar situations. Conclusion: We show the power of semantic annotations in an adequately modelled and semantically enabled domain setting. Using model checking and synthesis methods, users may orchestrate complex processes from a wealth of heterogeneous services without worrying about interfaces and (type) consistency. The success of this method strongly depends on a careful semantic annotation of the provided services and on its consequent exploitation for analysis, validation, and synthesis. We are convinced that these annotations will become standard, as they will become preconditions for the success and widespread use of (preferred) services in the Semantic Web T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 136 KW - European Bioinformatics Institute KW - Integration KW - Tool KW - Alignment KW - Workflow Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45066 ER - TY - GEN A1 - Li, Zhonghao A1 - Taubert, Andreas T1 - Cellulose/gold nanocrystal hybrids via an ionic liquid/aqueous precipitation route N2 - Injection of a mixture of HAuCl4 and cellulose dissolved in the ionic liquid (IL) 1-butyl-3-methylimidazolium chloride [Bmim]Cl into aqueous NaBH4 leads to colloidal gold nanoparticle/cellulose hybrid precipitates. This process is a model example for a very simple and generic approach towards (noble) metal/cellulose hybrids, which could find applications in sensing, sterile filtration, or as biomaterials. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 135 KW - Cellulose KW - Gold nanoparticles KW - Ionic liquid KW - Precipitation KW - Hybrid material Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45046 ER - TY - GEN A1 - Schumann, Silvia A1 - Terao, Mineko A1 - Garattini, Enrico A1 - Saggu, Miguel A1 - Lendzian, Friedhelm A1 - Hildebrandt, Peter A1 - Leimkühler, Silke T1 - Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX1 N2 - Mouse aldehyde oxidase (mAOX1) forms a homodimer and belongs to the xanthine oxidase family of molybdoenzymes which are characterized by an essential equatorial sulfur ligand coordinated to the molybdenum atom. In general, mammalian AOs are characterized by broad substrate specificity and an yet obscure physiological function. To define the physiological substrates and the enzymatic characteristics of mAOX1, we established a system for the heterologous expression of the enzyme in Eschericia coli. The recombinant protein showed spectral features and a range of substrate specificity similar to the native protein purified from mouse liver. The EPR data of recombinant mAOX1 were similar to those of AO from rabbit liver, but differed from the homologous xanthine oxidoreductase enzymes. Site-directed mutagenesis of amino acids Val806, Met884 and Glu1265 at the active site resulted in a drastic decrease in the oxidation of aldehydes with no increase in the oxidation of purine substrates. The double mutant V806E/M884R and the single mutant E1265Q were catalytically inactive enzymes regardless of the aldehyde or purine substrates tested. Our results show that only Glu1265 is essential for the catalytic activity by initiating the base-catalyzed mechanism of substrate oxidation. In addition, it is concluded that the substrate specificity of molybdo-flavoenzymes is more complex and not only defined by the three characterized amino acids in the active site. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 134 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45030 ER - TY - GEN A1 - Kröller, Katja A1 - Warschburger, Petra T1 - Maternal feeding strategies and child's food intake : considering weight and demographic influences using structural equation modeling N2 - Background: Research concerning child's food intake have considered various influencing factors, for example parental feeding strategies, demographic and weight factors. At this time, however, there are few findings that explore these factors simultaneously. Accordingly, the aim of this study was to test a structural equation model regarding the associations between maternal feeding strategies and child's food intake. Methods: 556 mothers and their children between 1 and 10 years of age participated in this crosssectional study. Besides socio-demographic and weight data, the mothers were asked about their feeding strategies as well as their child's food intake. Results: The well-fitting model explained 73% of the variance in the child's consumption of healthy and 34% of unhealthy food. In addition to the effect of the mother's social status and the child's age, a rewarding and modeling feeding behavior significantly influenced the child's food intake. Conclusion: The results highlight the relevance of maternal feeding behavior on the child's food intake. In terms of preventing eating- or weight-related problems, the findings indicate the usefulness of training parents in explicit modeling behavior and avoiding food as a reward. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - paper 181 KW - Parenting practices KW - Obesity risk KW - Consumption KW - Overweight KW - Adolescents Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45023 ER - TY - GEN A1 - Kugler, Annette A1 - Köhler, Barbara A1 - Palme, Klaus A1 - Wolff, Patricia A1 - Dietrich, Petra T1 - Salt-dependent regulation of a CNG channel subfamily in Arabidopsis N2 - Background: In Arabidopsis thaliana, the family of cyclic nucleotide-gated channels (CNGCs) is composed of 20 members. Previous studies indicate that plant CNGCs are involved in the control of growth processes and responses to abiotic and biotic stresses. According to their proposed function as cation entry pathways these channels contribute to cellular cation homeostasis, including calcium and sodium, as well as to stress-related signal transduction. Here, we studied the expression patterns and regulation of CNGC19 and CNGC20, which constitute one of the five CNGC subfamilies. Results: GUS, GFP and luciferase reporter assays were used to study the expression of CNGC19 and CNGC20 genes from Arabidopsis thaliana in response to developmental cues and salt stress. CNGC19 and CNGC20 were differentially expressed in roots and shoots. The CNGC19 gene was predominantly active in roots already at early growth stages. Major expression was observed in the phloem. CNGC20 showed highest promoter activity in mesophyll cells surrounding the veins. Its expression increased during development and was maximal in mature and senescent leaves. Both genes were upregulated in the shoot in response to elevated NaCl but not mannitol concentrations. While in the root, CNGC19 did not respond to changes in the salt concentration, in the shoot it was strongly upregulated in the observed time frame (6-72 hours). Salt-induction of CNGC20 was also observed in the shoot, starting already one hour after stress treatment. It occurred with similar kinetics, irrespective of whether NaCl was applied to roots of intact plants or to the petiole of detached leaves. No differences in K and Na contents of the shoots were measured in homozygous T-DNA insertion lines for CNGC19 and CNGC20, respectively, which developed a growth phenotype in the presence of up to 75 mM NaCl similar to that of the wild type. Conclusion: Together, the results strongly suggest that both channels are involved in the salinity response of different cell types in the shoot. Upon salinity both genes are upregulated within hours. CNGC19 and CNGC20 could assist the plant to cope with toxic effects caused by salt stress, probably by contributing to a re-allocation of sodium within the plant. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 133 KW - Nucleotide-gated channel KW - Na+/H+ antiporter SOS1 KW - Ricinus-communis l KW - Plasma membrane KW - Functional analysis Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45019 ER - TY - GEN A1 - Zhang, Gong A1 - Ignatova, Zoya T1 - Generic algorithm to predict the speed of translational elongation : implications for protein biogenesis N2 - Synonymous codon usage and variations in the level of isoaccepting tRNAs exert a powerful selective force on translation fidelity. We have developed an algorithm to evaluate the relative rate of translation which allows large-scale comparisons of the non-uniform translation rate on the protein biogenesis. Using the complete genomes of Escherichia coli and Bacillus subtilis we show that stretches of codons pairing to minor tRNAs form putative sites to locally attenuate translation; thereby the tendency is to cluster in near proximity whereas long contiguous stretches of slow-translating triplets are avoided. The presence of slow-translating segments positively correlates with the protein length irrespective of the protein abundance. The slow-translating clusters are predominantly located down-stream of the domain boundaries presumably to fine-tune translational accuracy with the folding fidelity of multidomain proteins. Translation attenuation patterns at highly structurally and functionally conserved domains are preserved across the species suggesting a concerted selective pressure on the codon selection and species-specific tRNA abundance in these regions. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 132 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-45007 ER - TY - GEN A1 - Hayn, Michael A1 - Beirle, Steffen A1 - Hamprecht, Fred A. A1 - Platt, Ulrich A1 - Menze, Björn H. A1 - Wagner, Thomas T1 - Analysing spatio-temporal patterns of the global NO2-distribution retrieved from GOME satellite observations using a generalized additive model N2 - With the increasing availability of observational data from different sources at a global level, joint analysis of these data is becoming especially attractive. For such an analysis – oftentimes with little prior knowledge about local and global interactions between the different observational variables at hand – an exploratory, data-driven analysis of the data may be of particular relevance. In the present work we used generalized additive models (GAM) in an exemplary study of spatio-temporal patterns in the tropospheric NO2-distribution derived from GOME satellite observations (1996 to 2001) at global scale. We focused on identifying correlations between NO2 and local wind fields, a quantity which is of particular interest in the analysis of spatio-temporal interactions. Formulating general functional, parametric relationships between the observed NO2 distribution and local wind fields, however, is difficult – if not impossible. So, rather than following a modelbased analysis testing the data for predefined hypotheses (assuming, for example, sinusoidal seasonal trends), we used a GAM with non-parametric model terms to learn this functional relationship between NO2 and wind directly from the data. The NO2 observations showed to be affected by winddominated processes over large areas. We estimated the extent of areas affected by specific NO2 emission sources, and were able to highlight likely atmospheric transport “pathways”. General temporal trends which were also part of our model – weekly, seasonal and linear changes – showed to be in good agreement with previous studies and alternative ways of analysing the time series. Overall, using a non-parametric model provided favorable means for a rapid inspection of this large spatio-temporal NO2 data set, with less bias than parametric approaches, and allowing to visualize dynamical processes of the NO2 distribution at a global scale. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 131 KW - Tropospheric nitrogen-dioxide KW - Air-pollution KW - Anthropogenic sources KW - NO2 KW - Validation Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44999 ER - TY - GEN A1 - Kartal, Önder A1 - Ebenhöh, Oliver T1 - Ground state robustness as an evolutionary design principle in signaling networks N2 - The ability of an organism to survive depends on its capability to adapt to external conditions. In addition to metabolic versatility and efficient replication, reliable signal transduction is essential. As signaling systems are under permanent evolutionary pressure one may assume that their structure reflects certain functional properties. However, despite promising theoretical studies in recent years, the selective forces which shape signaling network topologies in general remain unclear. Here, we propose prevention of autoactivation as one possible evolutionary design principle. A generic framework for continuous kinetic models is used to derive topological implications of demanding a dynamically stable ground state in signaling systems. To this end graph theoretical methods are applied. The index of the underlying digraph is shown to be a key topological property which determines the so-called kinetic ground state (or off-state) robustness. The kinetic robustness depends solely on the composition of the subdigraph with the strongly connected components, which comprise all positive feedbacks in the network. The component with the highest index in the feedback family is shown to dominate the kinetic robustness of the whole network, whereas relative size and girth of these motifs are emphasized as important determinants of the component index. Moreover, depending on topological features, the maintenance of robustness differs when networks are faced with structural perturbations. This structural off-state robustness, defined as the average kinetic robustness of a network’s neighborhood, turns out to be useful since some structural features are neutral towards kinetic robustness, but show up to be supporting against structural perturbations. Among these are a low connectivity, a high divergence and a low path sum. All results are tested against real signaling networks obtained from databases. The analysis suggests that ground state robustness may serve as a rationale for some structural peculiarities found in intracellular signaling networks. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 130 KW - Complex networks KW - Transduction KW - Motifs KW - Cell KW - Organization Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44982 ER - TY - GEN A1 - Ebenhöh, Oliver A1 - Handorf, Thomas T1 - Functional classification of genome-scale metabolic networks N2 - We propose two strategies to characterize organisms with respect to their metabolic capabilities. The first, investigative, strategy describes metabolic networks in terms of their capability to utilize different carbon sources, resulting in the concept of carbon utilization spectra. In the second, predictive, approach minimal nutrient combinations are predicted from the structure of the metabolic networks, resulting in a characteristic nutrient profile. Both strategies allow for a quantification of functional properties of metabolic networks, allowing to identify groups of organisms with similar functions. We investigate whether the functional description reflects the typical environments of the corresponding organisms by dividing all species into disjoint groups based on whether they are aerotolerant and/or photosynthetic. Despite differences in the underlying concepts, both measures display some common features. Closely related organisms often display a similar functional behavior and in both cases the functional measures appear to correlate with the considered classes of environments. Carbon utilization spectra and nutrient profiles are complementary approaches toward a functional classification of organism-wide metabolic networks. Both approaches contain different information and thus yield different clusterings, which are both different from the classical taxonomy of organisms. Our results indicate that a sophisticated combination of our approaches will allow for a quantitative description reflecting the lifestyles of organisms. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 129 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44975 ER - TY - GEN A1 - de Castro, Francisco A1 - Gaedke, Ursula A1 - Boenigk, Jens T1 - Reverse evolution : driving forces behind the loss of acquired photosynthetic traits N2 - Background: The loss of photosynthesis has occurred often in eukaryotic evolution, even more than its acquisition, which occurred at least nine times independently and which generated the evolution of the supergroups Archaeplastida, Rhizaria, Chromalveolata and Excavata. This secondary loss of autotrophic capability is essential to explain the evolution of eukaryotes and the high diversity of protists, which has been severely underestimated until recently. However, the ecological and evolutionary scenarios behind this evolutionary ‘‘step back’’ are still largely unknown. Methodology/Principal Findings: Using a dynamic model of heterotrophic and mixotrophic flagellates and two types of prey, large bacteria and ultramicrobacteria, we examine the influence of DOC concentration, mixotroph’s photosynthetic growth rate, and external limitations of photosynthesis on the coexistence of both types of flagellates. Our key premises are: large bacteria grow faster than small ones at high DOC concentrations, and vice versa; and heterotrophic flagellates are more efficient than the mixotrophs grazing small bacteria (both empirically supported). We show that differential efficiency in bacteria grazing, which strongly depends on cell size, is a key factor to explain the loss of photosynthesis in mixotrophs (which combine photosynthesis and bacterivory) leading to purely heterotrophic lineages. Further, we show in what conditions an heterotroph mutant can coexist, or even out-compete, its mixotrophic ancestor, suggesting that bacterivory and cell size reduction may have been major triggers for the diversification of eukaryotes. Conclusions/Significance: Our results suggest that, provided the mixotroph’s photosynthetic advantage is not too large, the (small) heterotroph will also dominate in nutrient-poor environments and will readily invade a community of mixotrophs and bacteria, due to its higher efficiency exploiting the ultramicrobacteria. As carbon-limited conditions were presumably widespread throughout Earth history, such a scenario may explain the numerous transitions from phototrophy to mixotrophy and further to heterotrophy within virtually all major algal lineages. We challenge prevailing concepts that affiliated the evolution of phagotrophy with eutrophic or strongly light-limited environments only. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 128 KW - Fresh-water habitats KW - Operon copy number KW - Growth-rate KW - Bacteria KW - Plankton Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44968 ER - TY - GEN A1 - Dambacher, Michael A1 - Rolfs, Martin A1 - Göllner, Kristin A1 - Kliegl, Reinhold A1 - Jacobs, Arthur M. T1 - Event-related potentials reveal rapid verification of predicted visual input N2 - Human information processing depends critically on continuous predictions about upcoming events, but the temporal convergence of expectancy-based top-down and input-driven bottom-up streams is poorly understood. We show that, during reading, event-related potentials differ between exposure to highly predictable and unpredictable words no later than 90 ms after visual input. This result suggests an extremely rapid comparison of expected and incoming visual information and gives an upper temporal bound for theories of top-down and bottom-up interactions in object recognition. T3 - Zweitveröffentlichungen der Universität Potsdam : Humanwissenschaftliche Reihe - paper 180 KW - Interactive activation model KW - Top-down influences KW - Word form area KW - Spatial attention KW - Brain potentials Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44953 ER - TY - GEN A1 - May, Patrick A1 - Christian, Jan-Ole A1 - Kempa, Stefan A1 - Walther, Dirk T1 - ChlamyCyc : an integrative systems biology database and web-portal for Chlamydomonas reinhardtii N2 - Background: The unicellular green alga Chlamydomonas reinhardtii is an important eukaryotic model organism for the study of photosynthesis and plant growth. In the era of modern highthroughput technologies there is an imperative need to integrate large-scale data sets from highthroughput experimental techniques using computational methods and database resources to provide comprehensive information about the molecular and cellular organization of a single organism. Results: In the framework of the German Systems Biology initiative GoFORSYS, a pathway database and web-portal for Chlamydomonas (ChlamyCyc) was established, which currently features about 250 metabolic pathways with associated genes, enzymes, and compound information. ChlamyCyc was assembled using an integrative approach combining the recently published genome sequence, bioinformatics methods, and experimental data from metabolomics and proteomics experiments. We analyzed and integrated a combination of primary and secondary database resources, such as existing genome annotations from JGI, EST collections, orthology information, and MapMan classification. Conclusion: ChlamyCyc provides a curated and integrated systems biology repository that will enable and assist in systematic studies of fundamental cellular processes in Chlamydomonas. The ChlamyCyc database and web-portal is freely available under http://chlamycyc.mpimp-golm.mpg.de. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 127 KW - Biochemical pathway database KW - Gene-expression data KW - Quantitative proteomics KW - Metabolic pathways KW - Genome annotation Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44947 ER - TY - GEN A1 - Bürger, Gerd T1 - Dynamically vs. empirically downscaled medium-range precipitation forecasts N2 - For three small, mountainous catchments in Germany two medium-range forecast systems are compared that predict precipitation for up to 5 days in advance. One system is composed of the global German weather service (DWD) model, GME, which is dynamically downscaled using the COSMO-EU regional model. The other system is an empirical (expanded) downscaling of the ECMWF model IFS. Forecasts are verified against multi-year daily observations, by applying standard skill scores to events of specified intensity. All event classes are skillfully predicted by the empirical system for up to five days lead time. For the available prediction range of one to two days it is superior to the dynamical system. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 126 KW - Weather-service KW - Verification KW - Scenarios KW - Models Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44939 ER - TY - GEN A1 - Blume, Theresa A1 - Zehe, Erwin A1 - Bronstert, Axel T1 - Use of soil moisture dynamics and patterns at different spatio-temporal scales for the investigation of subsurface flow processes N2 - Spatial patterns as well as temporal dynamics of soil moisture have a major influence on runoff generation. The investigation of these dynamics and patterns can thus yield valuable information on hydrological processes, especially in data scarce or previously ungauged catchments. The combination of spatially scarce but temporally high resolution soil moisture profiles with episodic and thus temporally scarce moisture profiles at additional locations provides information on spatial as well as temporal patterns of soil moisture at the hillslope transect scale. This approach is better suited to difficult terrain (dense forest, steep slopes) than geophysical techniques and at the same time less cost-intensive than a high resolution grid of continuously measuring sensors. Rainfall simulation experiments with dye tracers while continuously monitoring soil moisture response allows for visualization of flow processes in the unsaturated zone at these locations. Data was analyzed at different spacio-temporal scales using various graphical methods, such as space-time colour maps (for the event and plot scale) and binary indicator maps (for the long-term and hillslope scale). Annual dynamics of soil moisture and decimeterscale variability were also investigated. The proposed approach proved to be successful in the investigation of flow processes in the unsaturated zone and showed the importance of preferential flow in the Malalcahuello Catchment, a datascarce catchment in the Andes of Southern Chile. Fast response times of stream flow indicate that preferential flow observed at the plot scale might also be of importance at the hillslope or catchment scale. Flow patterns were highly variable in space but persistent in time. The most likely explanation for preferential flow in this catchment is a combination of hydrophobicity, small scale heterogeneity in rainfall due to redistribution in the canopy and strong gradients in unsaturated conductivities leading to self-reinforcing flow paths. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 125 KW - Repellent sandy soil KW - Poorly gauged catchment KW - Volcanic ash soils KW - Water repellency KW - Preferential flow Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44924 ER - TY - GEN A1 - Mwinyi, Adina A1 - Meyer, Achim A1 - Bleidorn, Christoph A1 - Lieb, Bernhard A1 - Bartolomaeus, Thomas A1 - Podsiadlowski, Lars T1 - Mitochondrial genome sequence and gene order of Sipunculus nudus give additional support for an inclusion of Sipuncula into Annelida N2 - Background: Mitochondrial genomes are a valuable source of data for analysing phylogenetic relationships. Besides sequence information, mitochondrial gene order may add phylogenetically useful information, too. Sipuncula are unsegmented marine worms, traditionally placed in their own phylum. Recent molecular and morphological findings suggest a close affinity to the segmented Annelida. Results: The first complete mitochondrial genome of a member of Sipuncula, Sipunculus nudus, is presented. All 37 genes characteristic for metazoan mtDNA were detected and are encoded on the same strand. The mitochondrial gene order (protein-coding and ribosomal RNA genes) resembles that of annelids, but shows several derivations so far found only in Sipuncula. Sequence based phylogenetic analysis of mitochondrial protein-coding genes results in significant bootstrap support for Annelida sensu lato, combining Annelida together with Sipuncula, Echiura, Pogonophora and Myzostomida. Conclusion: The mitochondrial sequence data support a close relationship of Annelida and Sipuncula. Also the most parsimonious explanation of changes in gene order favours a derivation from the annelid gene order. These results complement findings from recent phylogenetic analyses of nuclear encoded genes as well as a report of a segmental neural patterning in Sipuncula. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 124 KW - Transfer-rna genes; Phylogenetic analysis; Animal phylogeny; Control region; Sister Taxau Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44916 ER - TY - GEN A1 - Bleidorn, Christoph A1 - Podsiadlowski, Lars A1 - Zhong, Min A1 - Eeckhaut, Igor A1 - Hartmann, Stefanie A1 - Halanych, Kenneth M. A1 - Tiedemann, Ralph T1 - On the phylogenetic position of Myzostomida : can 77 genes get it wrong? N2 - Background: Phylogenomic analyses recently became popular to address questions about deep metazoan phylogeny. Ribosomal proteins (RP) dominate many of these analyses or are, in some cases, the only genes included. Despite initial hopes, hylogenomic analyses including tens to hundreds of genes still fail to robustly place many bilaterian taxa. Results: Using the phylogenetic position of myzostomids as an example, we show that phylogenies derived from RP genes and mitochondrial genes produce incongruent results. Whereas the former support a position within a clade of platyzoan taxa, mitochondrial data recovers an annelid affinity, which is strongly supported by the gene order data and is congruent with morphology. Using hypothesis testing, our RP data significantly rejects the annelids affinity, whereas a platyzoan relationship is significantly rejected by the mitochondrial data. Conclusion: We conclude (i) that reliance of a set of markers belonging to a single class of macromolecular complexes might bias the analysis, and (ii) that concatenation of all available data might introduce conflicting signal into phylogenetic analyses. We therefore strongly recommend testing for data incongruence in phylogenomic analyses. Furthermore, judging all available data, we consider the annelid affinity hypothesis more plausible than a possible platyzoan affinity for myzostomids, and suspect long branch attraction is influencing the RP data. However, this hypothesis needs further confirmation by future analyses. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 123 KW - Cirriferum myzostomida KW - Mitochondrial genomes KW - Transfer-rna KW - Data sets KW - Sequence Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44893 ER - TY - GEN A1 - Bircken, Margrid T1 - Grünzweig, Walter ; Seeber, Ursula (Hrsg.): Fred Wander : Leben und Werk / [rezensiert von] Margrid Bircken N2 - rezensiertes Werk: Fred Wander : Leben und Werk / Walter Grünzweig und Ursula Seeber (Hrsg.). - Bonn : Weidle, c 2005. - 256 S. ISBN 3-931135-88-8 T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 74 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44593 ER - TY - GEN A1 - Bircken, Margrid T1 - Sehend werden T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 73 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44564 ER - TY - GEN A1 - Bircken, Margrid T1 - Hörnigk, Therese (Hrsg.): Sich aussetzen : das Wort ergreifen ; Texte und Bilder zum 80.Geburtstag von Christa Wolf / [rezensiert von] Margrid Bircken N2 - rezensiertes Werk: Sich aussetzen : das Wort ergreifen ; Texte und Bilder zum 80. Geburtstag von Christa Wolf / hrsg. von Therese Hörnigk im Auftr. des Literaturforums im Brecht-Haus und in Zusammenarbeit mit der Stiftung Preußische Seehandlung. - Göttingen : Wallstein-Verl., 2009. - 192 S. : Ill. ISBN 978-3-8353-0481-9 T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 72 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44550 ER - TY - GEN A1 - Bircken, Margrid T1 - Wilhelm Fraenger (1890 bis 1964) und Anna Seghers (1900 bis 1983) T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 71 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44546 ER - TY - GEN A1 - Bircken, Margrid T1 - Pergande, Ingrid ; Kaufmann, Ulrich (Hrsg.): Gegen das große Umsonst : vierzig Jahre mit dem Dichter Volker Braun / [rezensiert von] Margrid Bircken N2 - rezensiertes Werk: Gegen das große Umsonst : vierzig Jahre mit dem Dichter Volker Braun / Ingrid Pergande ... (Hrsg.). - Berlin [u.a.] : Pergande und Kaufmann, 2009. - 335 S. : Ill. ISBN 978-3-00-027239-4 T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 70 Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44530 ER - TY - GEN A1 - Scholtka, Bettina A1 - Schneider, Mandy A1 - Melcher, Ralph A1 - Katzenberger, Tiemo A1 - Friedrich, Daniela A1 - Berghof-Jäger, Kornelia A1 - Scheppach, Wolfgang A1 - Steinberg, Pablo T1 - A gene marker panel covering the Wnt and the Ras-Raf-MEK-MAPK signalling pathways allows to detect gene mutations in 80% of early (UICC I) colon cancer stages in humans N2 - Background: Very recently a gene marker panel that allows the mutational analysis of APC, CTNNB1, B-RAF and K-RAS was conceived. The aim of the present study was to use the 4-gene marker panel covering the Wnt and Ras-Raf-MEK-MAPK signalling pathways to determine the percentage of sporadic colorectal carcinomas (CRC) carrying at least one of the four above-mentioned genes in a mutated form alone and/or in combination with microsatellite instability (MSI) and to compare the sensitivity of the gene marker panel used in this study with that of gene marker panels previously reported in the scientific literature. Methods: CTNNB1 and B-RAF were screened by PCR-single-strand conformation polymorphism analysis and K-RAS gene mutations by restriction fragment length polymorphism analysis. For the mutational analysis of the APC gene mutation cluster region (codons 1243–1567) direct DNA sequencing was performed. The U.S. National Cancer Institute microsatellite panel (BAT25, BAT26, D2S123, D5S346 and D17S250) was used for MSI analysis. Results: It could be shown that about 80% of early stage CRC (UICC stages I and II) and over 90% of CRC in the UICC stage IV carried at least one mutated gene and/or showed MSI. No significant increase in the gene mutation frequencies could be determined when comparing tumours in the UICC stage I with those in UICC stage IV. Conclusions: When compared with previously published gene marker panels the 4-gene marker panel used in the present study shows an excellent performance, allowing to detect genetic alterations in 80–90% of human sporadic CRC samples analyzed. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 120 KW - Colorectal carcinomas KW - K-RAS KW - Microsatellite instability KW - Oncogenes KW - Tumour suppressor genes Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44587 ER - TY - GEN A1 - Scholtka, Bettina A1 - Kühnel, Dana A1 - Taugner, Felicitas A1 - Steinberg, Pablo T1 - Inflammation does not precede or accompany the induction of perneoplastic lesions in the colon of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-fed rats N2 - Heterocyclic aromatic amines (HCAs) are formed in meat cooked at high temperatures for a long time or over an open flame. In this context 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), the most abundant HCA in cooked meat, has been suggested to be involved in colon and prostate carcinogenesis. In the latter case it has been reported that: (1) roughly 50% of Fischer F344 male rats treated with PhIP develop carcinomas in the ventral prostate lobe at 1 year of age; (2) inflammation precedes prostatic intraepithelial neoplasia in PhIP-fed rats; (3) inflammation specifically occurs in the ventral prostate lobe of PhIP-fed rats. To test whether PhIP by itself leads to inflammation in the colon and whether a human-relevant concentration of PhIP is able to induce preneoplastic lesions in the colon, male F344 rats were fed 0.1 or 100 ppm PhIP for up to 10 months and thereafter the colon tissue was analyzed histochemically. In none of the experimental groups signs of acute or chronic colonic inflammation were observed. 0.1 ppm PhIP leads to the development of hyperplastic and dysplastic lesions in the colon of single animals, but the incidence of these lesions does not reach a statistical significance. In contrast, in rats fed 100 ppm PhIP for 10 months hyperplastic and dysplastic colonic lesions were induced in a statistically significant number of animals. It is concluded that: (1) the induction of preneoplastic lesions in rat colon by PhIP is not preceded or accompanied by an inflammatory process; (2) a human-relevant concentration of PhIP alone is not sufficient to initiate colon carcinogenesis in rats. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 119 KW - Colorectal cancer KW - Heterocyclic aromatic amines KW - Inflammation Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44570 ER - TY - GEN A1 - Peter, Martin G. A1 - Yenesew, Abiy A1 - Twinomuhwezi, Hannington A1 - Kabaru, Jacques M. A1 - Akala, Hoseah M. A1 - Kiremire, Bernard T. A1 - Heydenreich, Matthias A1 - Eyase, Fredrick A1 - Waters, Norman C. A1 - Walsh, Douglas S. T1 - Antiplasmodial and larvicidal flavonoids from Derris trifoliata N2 - From the dichloromethane-methanol (1:1) extract of the seed pods of Derris trifoliata, a new flavanone derivative (S)-lupinifolin 4´-methyl ether was isolated. In addition, the known flavonoids lupinifolin and rotenone were identified. The structures were determined on the basis of spectroscopic evidence. Lupinfolin showed moderate in vitro antiplasmodial activity against the D6 (chloroquine-sensitive) and W2 (chloroquineresistant) strains of Plasmodium falciparum. The different parts of this plant showed larvicidal activities against Aedes aegypti and rotenoids were identified as the active principles. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 121 KW - Derris trifoliata KW - Leguminosae KW - Flavanone KW - (S)-Lupinifolin 4´-methyl ether KW - Lupinifolin Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44614 ER - TY - GEN A1 - Eggert, Sandy A1 - Meier, Juliane T1 - Aktuelle Marktübersicht zum Funktionsumfang von Modellierungswerkzeugen N2 - In diesem Beitrag lesen Sie: • welche Ziele Modellierungstools haben können, • welche Objekte und Elemente generell abgebildet werden können, • welche unterschiedlichen Einsatzgebiete diese Tools haben können. T3 - Zweitveröffentlichungen der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe - paper 021 KW - Modellierungstools KW - Marktübersicht KW - Business Process Management Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44474 ER - TY - GEN A1 - Peter, Martin G. A1 - Muiva, Lois M. A1 - Yenesew, Abiy A1 - Derese, Solomon A1 - Heydenreich, Matthias A1 - Akala, Hoseah M. A1 - Eyase, Fredrick A1 - Waters, Norman C. A1 - Mutai, Charles A1 - Keriko, Joseph M. A1 - Walsh, Douglas S. T1 - Antiplasmodial β-hydroxydihydrochalcone from seedpods of Tephrosia elata N2 - From the seedpods of Tephrosia elata, a new β-hydroxydihydrochalcone named (S)-elatadihydrochalcone was isolated. In addition, the known flavonoids obovatachalcone, obovatin, obovatin methyl ether and deguelin were identified. The structures were determined on the basis of spectroscopic evidence. The crude extract and the flavonoids obtained from the seedpods of this plant showed antiplasmodial activities. The literature NMR data on β-hydroxydihydrochalcones is reviewed and the identity of some of the compounds assigned β-hydroxydihydrochalcone skeleton is questioned. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 118 KW - Tephrosia elata KW - Leguminosae KW - Seedpods KW - (S)-Elatadihydrochalcone KW - β-Hydroxydihydrochalcone Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44437 ER - TY - GEN A1 - Blenau, Wolfgang A1 - Rotte, Cathleen A1 - Witte, Jeannine A1 - Baumann, Otto A1 - Walz, Bernd T1 - Source, topography and excitatory effects of GABAergic innervation in cockroach salivary glands N2 - Cockroach salivary glands are innervated by dopaminergic and serotonergic neurons. Both transmitters elicit saliva secretion. We studied the distribution pattern of neurons containing gamma-aminobutyric acid ( GABA) and their physiological role. Immunofluorescence revealed a GABA-immunoreactive axon that originates within the subesophageal ganglion at the salivary neuron 2 (SN2) and this extends within the salivary duct nerve towards the salivary gland. GABA-positive fibers form a network on most acinar lobules and a dense plexus in the interior of a minor fraction of acinar lobules. Co-staining with anti-synapsin revealed that some putative GABAergic terminals seem to make pre-synaptic contacts with GABA-negative release sites. Many putative GABAergic release sites are at some distance from other synapses and at distance from the acinar tissue. Intracellular recordings from isolated salivary glands have revealed that GABA does not affect the basolateral membrane potential of the acinar cells directly. When applied during salivary duct nerve stimulation, GABA enhances the electrical response of the acinar cells and increases the rates of fluid and protein secretion. The effect on electrical cell responses is mimicked by the GABA(B) receptor agonists baclofen and SKF97541, and blocked by the GABAB receptor antagonists CGP52432 and CGP54626. These findings indicate that GABA has a modulatory role in the control of salivation, acting presynaptically on serotonergic and/or dopaminergic neurotransmission. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 111 KW - GABA KW - salivary gland KW - innervation KW - cockroach KW - Periplaneta americana Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-44353 ER - TY - GEN A1 - Tristram, Hildegard L.C. T1 - Wie weit sind die inselkeltischen Sprachen (und das Englische) analytisiert? T1 - How far have the Insular Celtic languages (and the English language) been analyticised? N2 - Der gemeinsame Wandel der inselkeltischen Sprachen wie auch des Englischen vom vorwiegend synthetischen Typus zum vorwiegend analytischen Typus läßt sich vermutlich auf einen ca. 1500 Jahre dauernden intensiven Sprachenkontakt zwischen diesen Sprachen zurückführen. Heute ist das Englische die analytischste Sprache der Britischen Inseln und Irlands, gefolgt vom Walisischen, Bretonischen und Irischen. Letzteres ist von den genannten Sprachen noch am weitesten morphologisch komplex. N2 - I discuss the joint shift of the Insular Celtic languages and of the English language from, typologically speaking, predominantly synthetic languages c. 1500 years ago to predominantly analytical languages today. The demise of the inflectional morphology is most advanced in Present Day English. Welsh follows suit. Then come Breton and Irish. Intensive linguistic interaction across the boundaries of the Germanic and the Insular Celtic languages are proposed to have been instrumental for this type of linguistic convergence. T3 - Zweitveröffentlichungen der Universität Potsdam : Philosophische Reihe - 35 KW - Irisch KW - Walisisch KW - Bretonisch KW - Englisch KW - Sprachwandel KW - Sprachkontakt KW - Sprachkonvergenz KW - Typologie KW - Morphologie KW - Komplexität KW - Quantifizierun KW - Irish KW - Welsh KW - Breton KW - English KW - Language Change KW - Language Contact KW - Convergence KW - Morphology KW - Complexity KW - Quantification Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-41251 ER - TY - GEN A1 - Misselwitz, Hans-Jürgen T1 - Das Zeitfenster ist wieder offen T1 - The time frame is open again N2 - Es sei eine "Sternstunde der Diplomatie" gewesen, die Lösung der deutschen Frage 1990. Alle waren überzeugt, dass die Lösung dieser Frage die Zustimmung aller Beteiligten erforderte und die dafür nötige Zeit. Was aber war das richtige Maß? KW - NATO KW - Russland KW - USA KW - NATO KW - Russia KW - USA Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-39531 SN - 978-3-86956-020-5 ER - TY - GEN T1 - Blattgold : Machtkerle 09 T1 - Blattgold : Power Fellows 09 N2 - Er regiert lustvoll. Kleiner Mann im Maßanzug. Der Typus des männlichen Politikers und Staatenlenkers, der sich im Rampenlicht einer fasziniert-angeödeten Öffentlichkeit exponiert, pflegt den Hang zur Selbstdarstellung. Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-39527 SN - 978-3-86956-020-5 ER - TY - GEN A1 - Franzke, Jochen T1 - Europäische Zeitenwenden T1 - European transitions N2 - Der Autor skizziert die europäischen Zeitenwenden vom Ausbruch des Zweiten Weltkrieges über das Wendejahr 1989 bis zur Gegenwart. Seine Überlegungen stützen sich auf die Konferenz "Das Jahr 1939: Ursprünge und Beginn des 2. Weltkriegs", die am 9. September 2009 in Posen stattgefunden hat. KW - Deutschland KW - Polen KW - Russland KW - Germany KW - Poland KW - Russia Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-39510 SN - 978386956020-5 ER - TY - GEN T1 - Europäische Brüche T1 - European breaklines N2 - Vor 70 Jahren begann der Zweite Weltkrieg, ihm folgte der Kalte Krieg, der mit dem Jahr 89 abschloss. Die Folgen dieser europäischen Brüche sind bis heute zu spüren, vor allem für das Dreieck Warschau - Moskau - Berlin. Raus oder rein? Im Forum Afghanistan wird die Frage nach dem Engagement "des Westens" mit Carl Schmitt zugespitzt. Die tödliche Realität dieses Krieges setzt sich gegen die von der politischen Klasse vereinbarte Tabuisierung durch. Die Verlängerung des Mandates der Bundeswehr steht demnächst an. Die politische Debatte muss geführt werden. Jetzt und hier! Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-39508 SN - 978-3-86956-020-5 ER - TY - GEN T1 - Außenpolitik in Schwarz-Rot N2 - Die Jahre der Großen Koalition neigen sich dem Ende zu. Bilanz wird gezogen, auch in der Außenpolitik. Ob Europäische Union oder die Beziehungen zu den USA, zu Polen, Russland oder China, ob im Krieg in Afghanistan oder beim Kampf gegen somalische Piraten – oft glich die deutsche Außenpolitik einem Drahtseilakt in den Turbulenzen dieser Welt. Im vorliegenden WeltTrends-Heft diskutieren Politiker und Wissenschaftler die Höhen und Tiefen, die Leistungen und die Defizite der Schwarz-Roten-Außenpolitik der letzten vier Jahre. Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-34275 SN - 978-3-940793-96-6 ER - TY - GEN T1 - NATO in der Sinnkrise T1 - NATO in identity crisis N2 - Abschaffen oder reformieren? Egal, wie die Frage beantwortet wird, die Zeit für die „alte“ NATO ist abgelaufen. Ob ihr ein strategischer Relaunch für das 21. Jahrhundert gelingt oder ob sie in den Bergen Afghanistan allmählich zerbröselt – das ist noch offen. Sicher ist jedoch, es bedarf der Debatte über die Zukunft dieser politisch-militärischen Organisation, von den Bürgern in allen NATO-Ländern und damit auch hier. Afghanistan, Abrüstung und Afrika sind weitere Themen dieses Heftes, das erneut zeigt, dass WeltTrends eine solide Plattform für streitbare Diskussionen um die deutsche Außenpolitik ist. KW - NATO KW - Außenpolitik KW - Abrüstung KW - NATO KW - Foreign Policy KW - Disarmament Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-33934 SN - 978-3-86956-007-6 ER - TY - GEN A1 - Sabrow, Sophia A1 - Kanyeihamba, George W. T1 - Menschenrechte auch in Afrika! : WT-Interview mit dem ugandischen Richter George W. Kanyeihamba T1 - Human Rights for Africa! : WT-Interview with George W. Kanyeihamba, Ugandan Judge N2 - Im Rahmen der alljährlichen Potsdamer Frühjahrsgespräche, die die Stiftung Entwicklung und Frieden in Kooperation mit WeltTrends durchführt, hatten wir Gelegenheit zu einem Gespräch mit dem ehemaligen Richter am Afrikanischen Gerichtshof für Menschenrechte und die Rechte der Völker, George W. Kanyeihamba aus Uganda. KW - Afrika KW - Menschenrechte KW - Interview KW - Africa KW - Human Rights KW - Interview Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-33957 SN - 0944-8101 ER -