TY  - JOUR
A1  - Laux, Eva-Maria
A1  - Ermilova, Elena
A1  - Pannwitz, Daniel
A1  - Gibbons, Jessica
A1  - Hölzel, Ralph
A1  - Bier, Frank Fabian
T1  - Dielectric Spectroscopy of Biomolecules up to 110 GHz
JF  - Frequenz
N2  - Radio-frequency fields in the GHz range are increasingly applied in biotechnology and medicine. In order to fully exploit both their potential and their risks detailed information about the dielectric properties of biological material is needed. For this purpose a measuring system is presented that allows the acquisition of complex dielectric spectra over 4 frequency decade up to 110 GHz. Routines for calibration and for data evaluation according to physicochemical interaction models have been developed. The frequency dependent permittivity and dielectric loss of some proteins and nucleic acids, the main classes of biomolecules, and of their sub-units have been determined. Dielectric spectra are presented for the amino acid alanine, the proteins lysozyme and haemoglobin, the nucleotides AMP and ATP, and for the plasmid pET-21, which has been produced by bacterial culture. Characterisation of a variety of biomolecules is envisaged, as is the application to studies on protein structure and function.
KW  - dielectric
KW  - spectroscopy
KW  - permittivity
KW  - protein
KW  - DNA
KW  - amino acid
KW  - plasmid
Y1  - 2018
U6  - https://doi.org/10.1515/freq-2018-0010
SN  - 0016-1136
SN  - 2191-6349
VL  - 72
IS  - 3-4
SP  - 135
EP  - 140
PB  - De Gruyter
CY  - Berlin
ER  - 
TY  - GEN
A1  - Wessig, Pablo
A1  - Bader, Denise
A1  - Klier, Dennis Tobias
A1  - Hettrich, Cornelia
A1  - Bier, Frank Fabian
T1  - Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes
N2  - Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein – a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 314 
KW  - conformational-changes
KW  - green-i
KW  - protein
KW  - binding
KW  - assay
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-394382
SP  - 1235
EP  - 1238
ER  -