TY  - JOUR
A1  - Bartholomäus, Alexander
A1  - Fedyunin, Ivan
A1  - Feist, Peter
A1  - Sin, Celine
A1  - Zhang, Gong
A1  - Valleriani, Angelo
A1  - Ignatova, Zoya
T1  - Bacteria differently regulate mRNA abundance to specifically respond to various stresses
JF  - Geology
N2  - Environmental stress is detrimental to cell viability and requires an adequate reprogramming of cellular activities to maximize cell survival. We present a global analysis of the response of Escherichia coli to acute heat and osmotic stress. We combine deep sequencing of total mRNA and ribosome-protected fragments to provide a genome-wide map of the stress response at transcriptional and translational levels. For each type of stress, we observe a unique subset of genes that shape the stress-specific response. Upon temperature upshift, mRNAs with reduced folding stability up-and downstream of the start codon, and thus with more accessible initiation regions, are translationally favoured. Conversely, osmotic upshift causes a global reduction of highly translated transcripts with high copy numbers, allowing reallocation of translation resources to not degraded and newly synthesized mRNAs.
KW  - transcription
KW  - translation
KW  - deep sequencing
KW  - Escherichia coli
KW  - copy numbers
Y1  - 2016
U6  - https://doi.org/10.1098/rsta.2015.0069
SN  - 1364-503X
SN  - 1471-2962
VL  - 374
PB  - Royal Society
CY  - London
ER  - 
TY  - JOUR
A1  - Baraza, Lilechi D.
A1  - Neser, Wekesa
A1  - Jackson, Korir Cheruiyot
A1  - Fredrick, Juma B.
A1  - Dennis, Ochieno
A1  - Wairimu, Kamau R.
A1  - Keya, Aggrey Osogo
A1  - Heydenreich, Matthias
T1  - Antimicrobial Coumarins from the Oyster Culinary-Medicinal Mushroom, Pleurotus ostreatus (Agaricomycetes), from Kenya
JF  - International journal of medicinal mushrooms
N2  - Pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ()streams against selected human pathogenic bacteria and endophytic fungi. Chemical structures were determined using spectroscopic methods and by comparison with values of related structures reported in the literature. Pure compounds from P. ostreatus were tested in vitro against pathogenic bacteria (Staphylococcus aureus and Escherichia coli) and endophytic fungi (Pencillium digitatum and Fusarium prolferatum). A new compound, (E)-5,7-dimethoxy-6-(3-methylbuta-1,3-dienyl)-2H-chromen-2-one (5-methoxy-(E)-suberodiene) (compound 2), along with ergosterol (compound I.) and 5,7-dimethoxy-6-(3-methylbut-2-enyl)-2H-chromen-2-one (toddaculin; compound 3), were isolated from the fruiting bodies of P. ostreatus. The growth of S. aureus,E proliferatum, and P. digitatum colonies was inhibited in media containing compound 2, with minimum inhibitory concentrations closely comparable to those of conventional antibiotics.
KW  - Escherichia coli
KW  - Fusarium proliferatum
KW  - medicinal mushrooms
KW  - Penicillium digitatum
KW  - Pleurotus ostreatus
KW  - Staphylococcus aureus
Y1  - 2016
U6  - https://doi.org/10.1615/IntJMedMushrooms.v18.i10.60
SN  - 1521-9437
SN  - 1940-4344
VL  - 18
SP  - 905
EP  - 913
PB  - Begell House
CY  - Danbury
ER  -