TY - JOUR A1 - Üstün, Suayib A1 - Sheikh, Arsheed A1 - Gimenez-Ibanez, Selena A1 - Jones, Alexandra A1 - Ntoukakis, Vardis A1 - Börnke, Frederik T1 - The Proteasome Acts as a Hub for Plant Immunity and Is Targeted by Pseudomonas Type III Effectors JF - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants N2 - Recent evidence suggests that the ubiquitin-proteasome system is involved in several aspects of plant immunity and that a range of plant pathogens subvert the ubiquitin-proteasome system to enhance their virulence. Here, we show that proteasome activity is strongly induced during basal defense in Arabidopsis (Arabidopsis thaliana). Mutant lines of the proteasome subunits RPT2a and RPN12a support increased bacterial growth of virulent Pseudomonas syringae pv tomato DC3000 (Pst) and Pseudomonas syringae pv maculicola ES4326. Both proteasome subunits are required for pathogen-associated molecular pattern-triggered immunity responses. Analysis of bacterial growth after a secondary infection of systemic leaves revealed that the establishment of systemic acquired resistance (SAR) is impaired in proteasome mutants, suggesting that the proteasome also plays an important role in defense priming and SAR. In addition, we show that Pst inhibits proteasome activity in a type III secretion-dependent manner. A screen for type III effector proteins from Pst for their ability to interfere with proteasome activity revealed HopM1, HopAO1, HopA1, and HopG1 as putative proteasome inhibitors. Biochemical characterization of HopM1 by mass spectrometry indicates that HopM1 interacts with several E3 ubiquitin ligases and proteasome subunits. This supports the hypothesis that HopM1 associates with the proteasome, leading to its inhibition. Thus, the proteasome is an essential component of pathogen-associated molecular pattern-triggered immunity and SAR, which is targeted by multiple bacterial effectors. Y1 - 2016 U6 - https://doi.org/10.1104/pp.16.00808 SN - 0032-0889 SN - 1532-2548 VL - 172 SP - 1941 EP - 1958 PB - American Society of Plant Physiologists CY - Rockville ER - TY - JOUR A1 - ´Cwiek-Kupczynska, Hanna A1 - Altmann, Thomas A1 - Arend, Daniel A1 - Arnaud, Elizabeth A1 - Chen, Dijun A1 - Cornut, Guillaume A1 - Fiorani, Fabio A1 - Frohmberg, Wojciech A1 - Junker, Astrid A1 - Klukas, Christian A1 - Lange, Matthias A1 - Mazurek, Cezary A1 - Nafissi, Anahita A1 - Neveu, Pascal A1 - van Oeveren, Jan A1 - Pommier, Cyril A1 - Poorter, Hendrik A1 - Rocca-Serra, Philippe A1 - Sansone, Susanna-Assunta A1 - Scholz, Uwe A1 - van Schriek, Marco A1 - Seren, Ümit A1 - Usadel, Bjorn A1 - Weise, Stephan A1 - Kersey, Paul A1 - Krajewski, Pawel T1 - Measures for interoperability of phenotypic data: minimum information requirements and formatting JF - Plant Methods N2 - Background: Plant phenotypic data shrouds a wealth of information which, when accurately analysed and linked to other data types, brings to light the knowledge about the mechanisms of life. As phenotyping is a field of research comprising manifold, diverse and time-consuming experiments, the findings can be fostered by reusing and combining existing datasets. Their correct interpretation, and thus replicability, comparability and interoperability, is possible provided that the collected observations are equipped with an adequate set of metadata. So far there have been no common standards governing phenotypic data description, which hampered data exchange and reuse. Results: In this paper we propose the guidelines for proper handling of the information about plant phenotyping experiments, in terms of both the recommended content of the description and its formatting. We provide a document called "Minimum Information About a Plant Phenotyping Experiment", which specifies what information about each experiment should be given, and a Phenotyping Configuration for the ISA-Tab format, which allows to practically organise this information within a dataset. We provide examples of ISA-Tab-formatted phenotypic data, and a general description of a few systems where the recommendations have been implemented. Conclusions: Acceptance of the rules described in this paper by the plant phenotyping community will help to achieve findable, accessible, interoperable and reusable data. KW - Data standardisation and formatting KW - Experimental metadata KW - Minimum information recommendations KW - Plant phenotyping KW - Experiment description Y1 - 2016 U6 - https://doi.org/10.1186/s13007-016-0144-4 SN - 1746-4811 VL - 12 PB - BioMed Central CY - London ER - TY - JOUR A1 - Zühlke, Martin A1 - Riebe, Daniel A1 - Beitz, Toralf A1 - Löhmannsröben, Hans-Gerd A1 - Andreotti, Sandro A1 - Reinert, Knut A1 - Zenichowski, Karl A1 - Diener, Marc T1 - High-performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data management, and applications JF - Journal of separation science N2 - The combination of high-performance liquid chromatography and electrospray ionization ion mobility spectrometry facilitates the two-dimensional separation of complex mixtures in the retention and drift time plane. The ion mobility spectrometer presented here was optimized for flow rates customarily used in high-performance liquid chromatography between 100 and 1500 mu L/min. The characterization of the system with respect to such parameters as the peak capacity of each time dimension and of the 2D spectrum was carried out based on a separation of a pesticide mixture containing 24 substances. While the total ion current chromatogram is coarsely resolved, exhibiting coelutions for a number of compounds, all substances can be separately detected in the 2D plane due to the orthogonality of the separations in retention and drift dimensions. Another major advantage of the ion mobility detector is the identification of substances based on their characteristic mobilities. Electrospray ionization allows the detection of substances lacking a chromophore. As an example, the separation of a mixture of 18 amino acids is presented. A software built upon the free mass spectrometry package OpenMS was developed for processing the extensive 2D data. The different processing steps are implemented as separate modules which can be arranged in a graphic workflow facilitating automated processing of data. KW - Amino acids KW - Electrospray ionization KW - Ion mobility spectrometry KW - Pesticides KW - Two-dimensional separations Y1 - 2016 U6 - https://doi.org/10.1002/jssc.201600749 SN - 1615-9306 SN - 1615-9314 VL - 39 SP - 4756 EP - 4764 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Zöller, Gert A1 - Holschneider, Matthias T1 - The Maximum Possible and the Maximum Expected Earthquake Magnitude for Production-Induced Earthquakes at the Gas Field in Groningen, The Netherlands JF - Bulletin of the Seismological Society of America N2 - The Groningen gas field serves as a natural laboratory for production-induced earthquakes, because no earthquakes were observed before the beginning of gas production. Increasing gas production rates resulted in growing earthquake activity and eventually in the occurrence of the 2012M(w) 3.6 Huizinge earthquake. At least since this event, a detailed seismic hazard and risk assessment including estimation of the maximum earthquake magnitude is considered to be necessary to decide on the future gas production. In this short note, we first apply state-of-the-art methods of mathematical statistics to derive confidence intervals for the maximum possible earthquake magnitude m(max). Second, we calculate the maximum expected magnitude M-T in the time between 2016 and 2024 for three assumed gas-production scenarios. Using broadly accepted physical assumptions and 90% confidence level, we suggest a value of m(max) 4.4, whereas M-T varies between 3.9 and 4.3, depending on the production scenario. Y1 - 2016 U6 - https://doi.org/10.1785/0120160220 SN - 0037-1106 SN - 1943-3573 VL - 106 SP - 2917 EP - 2921 PB - Seismological Society of America CY - Albany ER - TY - JOUR A1 - Zöller, Gert A1 - Holschneider, Matthias T1 - The Earthquake History in a Fault Zone Tells Us Almost Nothing about m(max) JF - Seismological research letters N2 - In the present study, we summarize and evaluate the endeavors from recent years to estimate the maximum possible earthquake magnitude m(max) from observed data. In particular, we use basic and physically motivated assumptions to identify best cases and worst cases in terms of lowest and highest degree of uncertainty of m(max). In a general framework, we demonstrate that earthquake data and earthquake proxy data recorded in a fault zone provide almost no information about m(max) unless reliable and homogeneous data of a long time interval, including several earthquakes with magnitude close to m(max), are available. Even if detailed earthquake information from some centuries including historic and paleoearthquakes are given, only very few, namely the largest events, will contribute at all to the estimation of m(max), and this results in unacceptably high uncertainties. As a consequence, estimators of m(max) in a fault zone, which are based solely on earthquake-related information from this region, have to be dismissed. Y1 - 2016 U6 - https://doi.org/10.1785/0220150176 SN - 0895-0695 SN - 1938-2057 VL - 87 SP - 132 EP - 137 PB - Seismological Society of America CY - Albany ER - TY - JOUR A1 - Zwickel, Theresa A1 - Kahl, Sandra M. A1 - Klaffke, Horst A1 - Rychlik, Michael A1 - Müller, Marina E. H. T1 - Spotlight on the Underdogs-An Analysis of Underrepresented Alternaria Mycotoxins Formed Depending on Varying Substrate, Time and Temperature Conditions JF - Toxins N2 - Alternaria (A.) is a genus of widespread fungi capable of producing numerous, possibly health-endangering Alternaria toxins (ATs), which are usually not the focus of attention. The formation of ATs depends on the species and complex interactions of various environmental factors and is not fully understood. In this study the influence of temperature (7 degrees C, 25 degrees C), substrate (rice, wheat kernels) and incubation time (4, 7, and 14 days) on the production of thirteen ATs and three sulfoconjugated ATs by three different Alternaria isolates from the species groups A. tenuissima and A. infectoria was determined. High-performance liquid chromatography coupled with tandem mass spectrometry was used for quantification. Under nearly all conditions, tenuazonic acid was the most extensively produced toxin. At 25 degrees C and with increasing incubation time all toxins were formed in high amounts by the two A. tenuissima strains on both substrates with comparable mycotoxin profiles. However, for some of the toxins, stagnation or a decrease in production was observed from day 7 to 14. As opposed to the A. tenuissima strains, the A. infectoria strain only produced low amounts of ATs, but high concentrations of stemphyltoxin III. The results provide an essential insight into the quantitative in vitro AT formation under different environmental conditions, potentially transferable to different field and storage conditions. KW - Alternaria infectoria KW - A. tenuissima KW - mycotoxin profile KW - wheat KW - rice KW - Alternaria toxin sulfates KW - modified Alternaria toxins KW - altertoxins KW - altenuic acid KW - HPLC-MS/MS Y1 - 2016 U6 - https://doi.org/10.3390/toxins8110344 SN - 2072-6651 VL - 8 SP - 570 EP - 583 PB - MDPI CY - Basel ER - TY - JOUR A1 - Zurnic, Irena A1 - Hütter, Sylvia A1 - Rzeha, Ute A1 - Stanke, Nicole A1 - Reh, Juliane A1 - Müllers, Erik A1 - Hamann, Martin V. A1 - Kern, Tobias A1 - Gerresheim, Gesche K. A1 - Lindel, Fabian A1 - Serrao, Erik A1 - Lesbats, Paul A1 - Engelman, Alan N. A1 - Cherepanov, Peter A1 - Lindemann, Dirk T1 - Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration JF - PLoS Pathogens N2 - Unlike for other retroviruses, only a few host cell factors that aid the replication of foamy viruses (FVs) via interaction with viral structural components are known. Using a yeast-two-hybrid (Y2H) screen with prototype FV (PFV) Gag protein as bait we identified human polo-like kinase 2 (hPLK2), a member of cell cycle regulatory kinases, as a new interactor of PFV capsids. Further Y2H studies confirmed interaction of PFV Gag with several PLKs of both human and rat origin. A consensus Ser-Thr/Ser-Pro (S-T/S-P) motif in Gag, which is conserved among primate FVs and phosphorylated in PFV virions, was essential for recognition by PLKs. In the case of rat PLK2, functional kinase and polo-box domains were required for interaction with PFV Gag. Fluorescently-tagged PFV Gag, through its chromatin tethering function, selectively relocalized ectopically expressed eGFP-tagged PLK proteins to mitotic chromosomes in a Gag STP motif-dependent manner, confirming a specific and dominant nature of the Gag-PLK interaction in mammalian cells. The functional relevance of the Gag-PLK interaction was examined in the context of replication-competent FVs and single-round PFV vectors. Although STP motif mutated viruses displayed wild type (wt) particle release, RNA packaging and intra-particle reverse transcription, their replication capacity was decreased 3-fold in single-cycle infections, and up to 20-fold in spreading infections over an extended time period. Strikingly similar defects were observed when cells infected with single-round wt Gag PFV vectors were treated with a pan PLK inhibitor. Analysis of entry kinetics of the mutant viruses indicated a post-fusion defect resulting in delayed and reduced integration, which was accompanied with an enhanced preference to integrate into heterochromatin. We conclude that interaction between PFV Gag and cellular PLK proteins is important for early replication steps of PFV within host cells. Y1 - 2016 U6 - https://doi.org/10.1371/journal.ppat.1005860 SN - 1553-7366 SN - 1553-7374 VL - 12 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Zohsel, Katrin A1 - Baldus, Christiane A1 - Schmidt, Martin H. A1 - Esser, Günter A1 - Banaschewski, Tobias A1 - Thomasius, Rainer A1 - Laucht, Manfred T1 - Predicting later problematic cannabis use from psychopathological symptoms during childhood and adolescence: Results of a 25-year longitudinal study JF - Drug and alcohol dependence : an international journal on biomedical and psychosocial approaches N2 - Background: Cannabis is the most commonly used illegal substance among adolescents and young adults. Problematic cannabis use is often associated with comorbid psychopathological problems. The purpose of the current study was to elucidate the underlying developmental processes connecting externalizing and internalizing psychopathology in childhood and adolescence with problematic cannabis use in young adulthood. Methods: Data were drawn from the Mannheim Study of Children at Risk, an ongoing epidemiological cohort study from birth to adulthood. For n = 307 participants, symptom scores of conduct/oppositional defiant disorder, attention problems, hyperactivity/impulsivity, and internalizing disorders were available for the periods of childhood (4.5-11 years) and adolescence (15 years). At age 25 years, problematic cannabis use was assessed via clinical interview and a self-rating questionnaire. Results: At age 25 years, problematic cannabis use was identified in n = 28 participants (9.1%). Childhood conduct/oppositional behavior problems were predictive of problematic cannabis use during young adulthood when comorbid symptoms were controlled for. No such effect was found for childhood attention, hyperactivity/impulsivity or internalizing problems. With respect to psychopathological symptoms during adolescence, only attention problems were significantly related to later problematic cannabis use when controlling for comorbidity. Conclusions: The current study highlights the role of conduct/oppositional behavior problems during childhood and attention problems during adolescence in later problematic cannabis use. It sheds more light on the developmental sequence of childhood and adolescence psychopathology and young adult cannabis use, which is a prerequisite for effective prevention approaches. (C) 2016 Elsevier Ireland Ltd. All rights reserved. KW - Problematic cannabis use KW - Externalizing behavior KW - Internalizing behavior KW - Childhood KW - Adolescence Y1 - 2016 U6 - https://doi.org/10.1016/j.drugalcdep.2016.04.012 SN - 0376-8716 SN - 1879-0046 VL - 163 SP - 251 EP - 255 PB - Elsevier CY - Clare ER - TY - JOUR A1 - Zimmermann, Malte T1 - Predicate focus JF - The Oxford handbook of information structure Y1 - 2016 SN - 978-0-19-964267-0 SP - 314 EP - 335 PB - Oxford University Press CY - Oxford ER - TY - CHAP A1 - Zimmermann, Andreas A1 - Geiß, Robin T1 - Article 8, VI, Article 8 para. 2 (f): scope of application of article 8 para. 2 (e) T2 - The Rome Statute of the International Criminal Court Y1 - 2016 SN - 978-3-406-64854-0 PB - Beck CY - München ER - TY - CHAP A1 - Zimmermann, Andreas A1 - Freiburg, Elisa ED - Triffterer, Otto ED - Ambos, Kai T1 - Article 15bis: Exercise of jurisdiction over the crime of aggression (State referal, proprio motu) T2 - The Rome Statute of the International Criminal Court ; a commentary Y1 - 2016 SN - 978-3-406-64854-0 SP - 741 EP - 764 PB - Beck CY - München ET - 3. Aufl ER - TY - CHAP A1 - Zimmermann, Andreas A1 - Freiburg, Elisa ED - Triffterer, Otto ED - Ambos, Kai T1 - Article 8bis: Crime of aggression T2 - The Rome Statute of the International Criminal Court ; a commentary Y1 - 2016 SN - 978-3-406-64854-0 SP - 580 EP - 618 PB - Beck CY - München ET - 3. Aufl. ER - TY - CHAP A1 - Zimmermann, Andreas A1 - Devaney, James G. T1 - Succession to treaties and the inherent limits of international law T2 - Research handbook on the law of treaties Y1 - 2016 SN - 978-1-78536-951-3 SP - 505 EP - 540 PB - Elgar CY - Cheltenham ER - TY - JOUR A1 - Zimmermann, Andreas T1 - Finally...Or would rather less have been more? JF - Journal of International Criminal Justice N2 - In November 2015, the 14th Session of the Assembly of States Parties to the Rome Statute of the International Criminal Court (ICC) adopted, by consensus, an amendment providing for the deletion of Article 124 of the ICC Statute, which so far enables contracting parties, when joining the Statute, to opt out from the ICC’s treaty-based war crimes-related jurisdiction. After considering the genesis of the provision and the practice arising under Article 124 of the ICC Statute so far, this article considers the arguments for and against the deletion of Article 124 in light of the increasingly small number of accessions to the ICC Statute that have been forthcoming in the last few years. It also analyses the quite strict requirements for the entry into force of the amendment, as well as the effect of the entry into force of the amendment on possible declarations having been made pending such entry into force. It ends by considering the positive effect a continued applicability of Article 124 may have on states so far being reluctant to accede to the ICC Statute. Y1 - 2016 U6 - https://doi.org/10.1093/jicj/mqw012 VL - 14 IS - 3 SP - 505 EP - 517 PB - Oxford University Press CY - Oxford ER - TY - CHAP A1 - Zimmermann, Andreas T1 - Article 8, D. Article 8 para 2 (c)-(f) and para 3: War crimes committed in an armed conflict not of an international character T2 - The Rome Statute of the International Criminal Court : a Commentary Y1 - 2016 SN - 978-3-8487-2263-1 SN - 978-3-8452-6357-1 U6 - https://doi.org/10.5771/9783845263571-296 SP - 296 EP - 580 PB - Beck CY - München ET - 3 ER - TY - CHAP A1 - Zimmermann, Andreas T1 - Article 5: Crimes within the Jurisdiction of the Court T2 - The Rome Statute of the International Criminal Court ; a commentary Y1 - 2016 SN - 978-3-406-64854-0 SP - 111 EP - 126 PB - Beck CY - München ET - 3. Aufl. ER - TY - JOUR A1 - Zimmermann, Andreas T1 - Finally ... Or Would Rather Less Have Been More? The Recent Amendment on the Deletion of Article 124 of the Rome Statute and the Continued Quest for the Universality of the International Criminal Court JF - Journal of international criminal justice N2 - In November 2015, the 14th Session of the Assembly of States Parties to the Rome Statute of the International Criminal Court (ICC) adopted, by consensus, an amendment providing for the deletion of Article 124 of the ICC Statute, which so far enables contracting parties, when joining the Statute, to opt out from the ICC’s treaty-based war crimes-related jurisdiction. After considering the genesis of the provision and the practice arising under Article 124 of the ICC Statute so far, this article considers the arguments for and against the deletion of Article 124 in light of the increasingly small number of accessions to the ICC Statute that have been forthcoming in the last few years. It also analyses the quite strict requirements for the entry into force of the amendment, as well as the effect of the entry into force of the amendment on possible declarations having been made pending such entry into force. It ends by considering the positive effect a continued applicability of Article 124 may have on states so far being reluctant to accede to the ICC Statute. Y1 - 2016 U6 - https://doi.org/10.1093/jicj/mqw012 SN - 1478-1387 SN - 1478-1395 VL - 14 SP - 505 EP - 517 PB - Oxford Univ. Press CY - Oxford ER - TY - JOUR A1 - Zimmermann, Alexander A1 - Voss, Sebastian A1 - Metzger, Johanna Clara A1 - Hildebrandt, Anke A1 - Zimmermann, Beate T1 - estimating mean throughfall JF - Journal of hydrology N2 - The selection of an appropriate spatial extent of a sampling plot is one among several important decisions involved in planning a throughfall sampling scheme. In fact, the choice of the extent may determine whether or not a study can adequately characterize the hydrological fluxes of the studied ecosystem. Previous attempts to optimize throughfall sampling schemes focused on the selection of an appropriate sample size, support, and sampling design, while comparatively little attention has been given to the role of the extent. In this contribution, we investigated the influence of the extent on the representativeness of mean throughfall estimates for three forest ecosystems of varying stand structure. Our study is based on virtual sampling of simulated throughfall fields. We derived these fields from throughfall data sampled in a simply structured forest (young tropical forest) and two heterogeneous forests (old tropical forest, unmanaged mixed European beech forest). We then sampled the simulated throughfall fields with three common extents and various sample sizes for a range of events and for accumulated data. Our findings suggest that the size of the study area should be carefully adapted to the complexity of the system under study and to the required temporal resolution of the throughfall data (i.e. event-based versus accumulated). Generally, event-based sampling in complex structured forests (conditions that favor comparatively long autocorrelations in throughfall) requires the largest extents. For event-based sampling, the choice of an appropriate extent can be as important as using an adequate sample size. (C) 2016 Elsevier B.V. All rights reserved. KW - Throughfall KW - Sampling KW - Extent KW - Hydrological monitoring KW - Tropical forest KW - European beech forest Y1 - 2016 U6 - https://doi.org/10.1016/j.jhydrol.2016.09.047 SN - 0022-1694 SN - 1879-2707 VL - 542 SP - 781 EP - 789 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ziegler, Moritz O. A1 - Rajabi, Mojtaba A1 - Heidbach, Oliver A1 - Hersir, Gylfi Pall A1 - Agustsson, Kristjan A1 - Arnadottir, Sigurveig A1 - Zang, Arno T1 - The stress pattern of Iceland JF - Tectonophysics : international journal of geotectonics and the geology and physics of the interior of the earth N2 - Iceland is located on the Mid-Atlantic Ridge which is the plate boundary between the Eurasian and the North American plates. It is one of the few places on earth where an active spreading centre is located onshore but the stress pattern has not been extensively investigated so far. In this paper we present a comprehensive compilation of the orientation of maximum horizontal stress (S-Hmax). In particular we interpret borehole breakouts and drilling induced fractures from borehole image logs in 57 geothermal wells onshore Iceland. The borehole results are combined with other stress indicators including earthquake focal mechanism solutions, geological information and overcoring measurements resulting in a dataset with 495 data records for the S-Hmax orientation. The reliability of each indicator is assessed according to the quality criteria of the World Stress Map project The majority of S-Hmax orientation data records in Iceland is derived from earthquake focal mechanism solutions (35%) and geological fault slip inversions (26%). 20% of the data are borehole related stress indicators. In addition minor shares of S-Hmax orientations are compiled, amongst others, from focal mechanism inversions and the alignment of fissure eruptions. The results show that the S-Hmax orientations derived from different depths and stress indicators are consistent with each other. The resulting pattern of the present-day stress in Iceland has four distinct subsets of S-Hmax orientations. The S-Hmax orientation is parallel to the rift axes in the vicinity of the active spreading regions. It changes from NE-SW in the South to approximately N-S in central Iceland and NNW-SSE in the North. In the Westfjords which is located far away from the ridge the regional S-Hmax rotates and is parallel to the plate motion. (C) 2016 Elsevier B.V. All rights reserved. KW - Iceland KW - Stress field KW - Stress pattern KW - Borehole image logs Y1 - 2016 U6 - https://doi.org/10.1016/j.tecto.2016.02.008 SN - 0040-1951 SN - 1879-3266 VL - 674 SP - 101 EP - 113 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Ziegler, Moritz O. A1 - Heidbach, Oliver A1 - Reinecker, John A1 - Przybycin, Anna M. A1 - Scheck-Wenderoth, Magdalena T1 - A multi-stage 3-D stress field modelling approach exemplified in the Bavarian Molasse Basin JF - Solid earth Y1 - 2016 U6 - https://doi.org/10.5194/se-7-1365-2016 SN - 1869-9510 SN - 1869-9529 VL - 7 SP - 1365 EP - 1382 PB - Copernicus CY - Göttingen ER - TY - JOUR A1 - Zibulski, Romy A1 - Herzschuh, Ulrike A1 - Pestryakova, Luidmila Agafyevna T1 - Vegetation patterns along micro-relief and vegetation type transects in polygonal landscapes of the Siberian Arctic JF - Journal of vegetation science N2 - QuestionHow important is the effect of micro-relief and vegetation type on the characteristics of vascular plants and bryophytes in low-centred polygons? LocationSiberian Arctic, Russia. MethodsEight low-centred polygons in northern Siberia were surveyed for vegetation along transects running from the rim to the pond via the rim-pond transition of each polygon and across a vegetation type gradient from open forest to tundra. ResultsThe cover of vascular plants and bryophytes displays no significant differences between the rim and rim-pond transition but is significantly lower in the pond section of the polygons. Alpha-diversity of vascular plants decreases strongly from rim to pond, whereas bryophyte diversity in pond plots is significantly distinct from the rim and the rim-pond transition. There is no clear trend in cover for either plant group along the vegetation type transect and only a weak trend in -diversity. However, both gradients are reflected in the compositional turnover. The applied indicator species analysis identified taxa characteristic of certain environmental conditions. Among others, we found vascular plants primarily characteristic of the rim and bryophyte taxa characteristic of each micro-relief level and vegetation type. ConclusionsThe observed gradual pattern in -diversity and composition of polygonal vegetation suggests that micro-relief is the main driver of changes in the vegetation composition, while vegetation type and the related forest cover change are of subordinate importance for polygonal vegetation patterns along the Siberian tree line. KW - Bryophytes KW - Indicator species KW - Low-centred polygon KW - NMDS KW - Russia KW - Tree line KW - Tundra KW - Vascular plants Y1 - 2016 U6 - https://doi.org/10.1111/jvs.12356 SN - 1100-9233 SN - 1654-1103 VL - 27 SP - 377 EP - 386 PB - Wiley-Blackwell CY - Hoboken ER - TY - JOUR A1 - Zhu, Fangjun A1 - Schlupp, Ingo A1 - Tiedemann, Ralph T1 - Sequence Evolution and Expression of the Androgen Receptor and Other Pathway-Related Genes in a Unisexual Fish, the Amazon Molly, Poecilia formosa, and Its Bisexual Ancestors JF - PLoS one N2 - The all-female Amazon molly (Poecilia formosa) originated from a single hybridization of two bisexual ancestors, Atlantic molly (Poecilia mexicana) and sailfin molly (Poecilia latipinna). As a gynogenetic species, the Amazon molly needs to copulate with a heterospecific male, but the genetic information of the sperm-donor does not contribute to the next generation, as the sperm only acts as the trigger for the diploid eggs’ embryogenesis. Here, we study the sequence evolution and gene expression of the duplicated genes coding for androgen receptors (ars) and other pathway-related genes, i.e., the estrogen receptors (ers) and cytochrome P450, family19, subfamily A, aromatase genes (cyp19as), in the Amazon molly, in comparison to its bisexual ancestors. Mollies possess–as most other teleost fish—two copies of the ar, er, and cyp19a genes, i.e., arα/arβ, erα/erβ1, and cyp19a1 (also referred as cyp19a1a)/cyp19a2 (also referred to as cyp19a1b), respectively. Non-synonymous single nucleotide polymorphisms (SNPs) among the ancestral bisexual species were generally predicted not to alter protein function. Some derived substitutions in the P. mexicana and one in P. formosa are predicted to impact protein function. We also describe the gene expression pattern of the ars and pathway-related genes in various tissues (i.e., brain, gill, and ovary) and provide SNP markers for allele specific expression research. As a general tendency, the levels of gene expression were lowest in gill and highest in ovarian tissues, while expression levels in the brain were intermediate in most cases. Expression levels in P. formosa were conserved where expression did not differ between the two bisexual ancestors. In those cases where gene expression levels significantly differed between the bisexual species, P. formosa expression was always comparable to the higher expression level among the two ancestors. Interestingly, erβ1 was expressed neither in brain nor in gill in the analyzed three molly species, which implies a more important role of erα in the estradiol synthesis pathway in these tissues. Furthermore, our data suggest that interactions of steroid-signaling pathway genes differ across tissues, in particular the interactions of ars and cyp19as. Y1 - 2016 U6 - https://doi.org/10.1371/journal.pone.0156209 SN - 1932-6203 VL - 11 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Zhu, Fangjun A1 - Schlupp, Ingo A1 - Tiedemann, Ralph T1 - Sequence Evolution and Expression of the Androgen Receptor and Other Pathway-Related Genes in a Unisexual Fish, the Amazon Molly, Poecilia formosa, and Its Bisexual Ancestors JF - PLoS one N2 - The all-female Amazon molly (Poecilia formosa) originated from a single hybridization of two bisexual ancestors, Atlantic molly (Poecilia mexicana) and sailfin molly (Poecilia latipinna). As a gynogenetic species, the Amazon molly needs to copulate with a heterospecific male, but the genetic information of the sperm-donor does not contribute to the next generation, as the sperm only acts as the trigger for the diploid eggs’ embryogenesis. Here, we study the sequence evolution and gene expression of the duplicated genes coding for androgen receptors (ars) and other pathway-related genes, i.e., the estrogen receptors (ers) and cytochrome P450, family19, subfamily A, aromatase genes (cyp19as), in the Amazon molly, in comparison to its bisexual ancestors. Mollies possess–as most other teleost fish—two copies of the ar, er, and cyp19a genes, i.e., arα/arβ, erα/erβ1, and cyp19a1 (also referred as cyp19a1a)/cyp19a2 (also referred to as cyp19a1b), respectively. Non-synonymous single nucleotide polymorphisms (SNPs) among the ancestral bisexual species were generally predicted not to alter protein function. Some derived substitutions in the P. mexicana and one in P. formosa are predicted to impact protein function. We also describe the gene expression pattern of the ars and pathway-related genes in various tissues (i.e., brain, gill, and ovary) and provide SNP markers for allele specific expression research. As a general tendency, the levels of gene expression were lowest in gill and highest in ovarian tissues, while expression levels in the brain were intermediate in most cases. Expression levels in P. formosa were conserved where expression did not differ between the two bisexual ancestors. In those cases where gene expression levels significantly differed between the bisexual species, P. formosa expression was always comparable to the higher expression level among the two ancestors. Interestingly, erβ1 was expressed neither in brain nor in gill in the analyzed three molly species, which implies a more important role of erα in the estradiol synthesis pathway in these tissues. Furthermore, our data suggest that interactions of steroid-signaling pathway genes differ across tissues, in particular the interactions of ars and cyp19as. Y1 - 2016 U6 - https://doi.org/10.1371/JOURNAL.PONE.0156209 SN - 1932-6203 VL - 11 IS - 6 PB - PLoS CY - Lawrence, Kan. ER - TY - THES A1 - Zhu, Fangjun T1 - Gene evolution and expression patterns in the all-female fish Amazon molly: Poecilia formosa Y1 - 2016 ER - TY - JOUR A1 - Zhou, Ying A1 - Zeng, Lanting A1 - Fu, Xiumin A1 - Mei, Xin A1 - Cheng, Sihua A1 - Liao, Yinyin A1 - Deng, Rufang A1 - Xu, Xinlan A1 - Jiang, Yueming A1 - Duan, Xuewu A1 - Baldermann, Susanne A1 - Yang, Ziyin T1 - The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato JF - Scientific reports N2 - The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato. Y1 - 2016 U6 - https://doi.org/10.1038/srep38742 SN - 2045-2322 VL - 6 PB - Nature Publ. Group CY - London ER - TY - JOUR A1 - Zhou, Renjie A1 - Schoenbohm, Lindsay M. A1 - Sobel, Edward A1 - Carrapa, Barbara A1 - Davis, Donald W. T1 - Sedimentary record of regional deformation and dynamics of the thick-skinned southern Puna Plateau, central Andes (26-27 degrees S) JF - Earth & planetary science letters N2 - The Puna Plateau, adjacent Eastern Cordillera and the Sierras Pampeanas of the central Andes are largely characterized by thick-skinned, basement-involved deformation. The Puna Plateau hosts similar to N-S trending bedrock ranges bounded by deep-seated reverse faults and sedimentary basins. We contribute to the understanding of thick-skinned dynamics in the Puna Plateau by constraining regional kinematics of the poorly understood southern Puna Plateau through a multidisciplinary approach. On the southeastern plateau, sandstone modal composition and detrital zircon U-Pb and apatite fission-track data from Cenozoic strata indicate basin accumulation during the late Eocene to early Oligocene (similar to 38-28 Ma). Provenance analysis reveals the existence of a regional-scale basin covering the southern Puna Plateau during late Eocene to early Oligocene time (similar to 38-28 Ma) that was sourced from both the western plateau and the eastern plateau margin and had a depocenter located to the west. Petrographic and detrital zircon U-Pb data reveal erosion of proximal western and eastern sources after 12 Ma, in mid-late Miocene time. This indicates that the regional basin was compartmentalized into small-scale depocenters by the growth of basement-cored ranges continuing into the late Miocene (similar to 12-8 Ma). We suggest that the Cenozoic history of the southern Puna Plateau records the formation of a regional basin that was possibly driven by lithospheric flexure during the late Eocene to early Oligocene, before the growth of distributed basement-cored ranges starting as early as the late Oligocene. (C) 2015 Elsevier B.V. All rights reserved. KW - Puna Plateau KW - regional deformation KW - basin analysis KW - thick-skinned deformation KW - zircon U-Pb geochronology KW - apatite fission-track thermochronology Y1 - 2016 U6 - https://doi.org/10.1016/j.epsl.2015.11.012 SN - 0012-821X SN - 1385-013X VL - 433 SP - 317 EP - 325 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Zhou, Qihui A1 - Wuennemann, Patrick A1 - Kuhn, Philipp Till A1 - de Vries, Joop A1 - Helmin, Marta A1 - Böker, Alexander A1 - van Kooten, Theo G. A1 - van Rijn, Patrick T1 - Mechanical Properties of Aligned Nanotopologies for Directing Cellular Behavior JF - Advanced materials interfaces N2 - Tailoring cell–surface interactions is important for the of design medical implants as well as regenerative medicine and tissue engineering materials. Here the single parameter system is transcended via translating hard nanotopology into soft polymeric hydrogel structures via hydrogel imprinting lithography. The response of these cells to the nanotopology of the same dimensions but with different mechanical properties displays unexpected behavior between “hard” tissue cells and “soft” tissue cells. Y1 - 2016 U6 - https://doi.org/10.1002/admi.201600275 SN - 2196-7350 VL - 3 PB - Wiley-Blackwell CY - Hoboken ER - TY - JOUR A1 - Zhou, Bin A1 - Lauwaet, Dirk A1 - Hooyberghs, Hans A1 - De Ridder, Koen A1 - Kropp, Jürgen A1 - Rybski, Diego T1 - Assessing Seasonality in the Surface Urban Heat Island of London JF - Journal of applied meteorology and climatology N2 - This paper assesses the seasonality of the urban heat island (UHI) effect in the Greater London area (United Kingdom). Combining satellite-based observations and urban boundary layer climate modeling with the UrbClim model, the authors are able to address the seasonality of UHI intensity, on the basis of both land surface temperature (LST) and 2-m air temperature, for four individual times of the day (0130, 1030, 1330, and 2230 local time) and the daily means derived from them. An objective of this paper is to investigate whether the UHI intensities that are based on both quantities exhibit a similar hysteresis-like trajectory that is observed for LST when plotting the UHI intensity against the background temperature. The results show that the UrbClim model can satisfactorily reproduce both the observed urban rural LSTs and 2-m air temperatures as well as their differences and the hysteresis in the surface UHI. The hysteresis-like seasonality is largely absent in both the observed and modeled 2-m air temperatures, however. A sensitivity simulation of the UHI intensity to incoming solar radiation suggests that the hysteresis of the LST can mainly be attributed to the seasonal variation in incoming solar radiation. Y1 - 2016 U6 - https://doi.org/10.1175/JAMC-D-15-0041.1 SN - 1558-8424 SN - 1558-8432 VL - 55 SP - 493 EP - 505 PB - American Meteorological Soc. CY - Boston ER - TY - JOUR A1 - Zhong, Qi A1 - Metwalli, Ezzeldin A1 - Rawolle, Monika A1 - Kaune, Gunar A1 - Bivigou Koumba, Achille Mayelle A1 - Laschewsky, Andre A1 - Papadakis, Christine M. A1 - Cubitt, Robert A1 - Wang, Jiping A1 - Müller-Buschbaum, Peter T1 - Influence of Hydrophobic Polystyrene Blocks on the Rehydration of Polystyrene-block-poly(methoxy diethylene glycol acrylate)-block-polystyrene Films Investigated by in Situ Neutron Reflectivity JF - Macromolecules : a publication of the American Chemical Society N2 - The rehydration of thermoresponsive polystyrene-block-poly(methoxy diethylene glycol acrylate)-block-polystyrene (PS-b-PMDEGA-b-PS) films forming a lamellar microphase-separated structure is investigated by in situ neutron reflectivity in a D2O vapor atmosphere. The rehydration of collapsed PS-b-PMDEGA-b-PS films is realized by a temperature change from 45 to 23 degrees C and comprises (1) condensation and absorption of D2O, (2) evaporation of D2O, and (3) reswelling of the film due to internal rearrangement. The hydrophobic PS layers hinder the absorption of condensed D2O, and a redistribution of embedded D2O between the hydrophobic PS layers and the hydrophilic PMDEGA layers is observed. In contrast, the rehydration of semiswollen PS-b-PMDEGA-b-PS films (temperature change from 35 to 23 degrees C) shows two prominent differences: A thicker D2O layer condenses on the surface, causing a more enhanced evaporation of D2O. The rehydrated films differ in film thickness and volume fraction of D2O, which is due to the different thermal protocols, although the final temperature is identical. Y1 - 2016 U6 - https://doi.org/10.1021/acs.macromol.5b02279 SN - 0024-9297 SN - 1520-5835 VL - 49 SP - 317 EP - 326 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Zhelavskaya, Irina A1 - Spasojevic, M. A1 - Shprits, Yuri Y. A1 - Kurth, William S. T1 - Automated determination of electron density from electric field measurements on the Van Allen Probes spacecraft JF - Journal of geophysical research : Space physics N2 - We present the Neural-network-based Upper hybrid Resonance Determination (NURD) algorithm for automatic inference of the electron number density from plasma wave measurements made on board NASA's Van Allen Probes mission. A feedforward neural network is developed to determine the upper hybrid resonance frequency, fuhr, from electric field measurements, which is then used to calculate the electron number density. In previous missions, the plasma resonance bands were manually identified, and there have been few attempts to do robust, routine automated detections. We describe the design and implementation of the algorithm and perform an initial analysis of the resulting electron number density distribution obtained by applying NURD to 2.5 years of data collected with the Electric and Magnetic Field Instrument Suite and Integrated Science (EMFISIS) instrumentation suite of the Van Allen Probes mission. Densities obtained by NURD are compared to those obtained by another recently developed automated technique and also to an existing empirical plasmasphere and trough density model. KW - Van Allen Probes KW - electron number density KW - neural networks Y1 - 2016 U6 - https://doi.org/10.1002/2015JA022132 SN - 2169-9380 SN - 2169-9402 VL - 121 SP - 4611 EP - 4625 PB - American Geophysical Union CY - Washington ER - TY - THES A1 - Zhang, Youjun T1 - Investigation of the TCA cycle and glycolytic metabolons and their physiological impacts in plants Y1 - 2016 ER - TY - JOUR A1 - Zhang, Shengrui A1 - Xu, Qinghai A1 - Gaillard, Marie-Jose A1 - Cao, Xianyong A1 - Li, Jianyong A1 - Zhang, Liyan A1 - Li, Yuecong A1 - Tian, Fang A1 - Zhou, Liping A1 - Lin, Fengyou A1 - Yang, Xiaolan T1 - Characteristic pollen source area and vertical pollen dispersal and deposition in a mixed coniferous and deciduous broad-leaved woodland in the Changbai mountains, northeast China JF - Vegetation History and Archaeobotany N2 - Pollen influx (number of pollen grains cm−2 year−1) can objectively reflect the dispersal and deposition features of pollen within a certain time and space, and is often used as a basis for the quantitative reconstruction of palaeovegetation; however, little is known about the features and mechanisms of vertical dispersal of pollen. Here we present the results from a 5 year (2006–2010) monitoring program using pollen traps placed at different heights from ground level up to 60 m and surface soil samples in a mixed coniferous and deciduous broad-leaved woodland in the Changbai mountains, northeastern China. The pollen percentages and pollen influx from the traps have very similar characteristics to the highest values for Betula, Fraxinus, Quercus and Pinus, among the tree taxa and Artemisia, Chenopodiaceae and Asteraceae among the herb taxa. Pollen influx values vary significantly with height and show major differences between three distinct layers, above-canopy (≥32 m), within the trunk layer (8 ≤ 32 m) and on the ground (0 m). These differences in pollen influx are explained by differences in (i) the air flows in each of these layers and (ii) the fall speed of pollen of the various taxa. We found that the pollen recorded on the ground surface is a good representation of the major part of the pollen transported in the trunk space of the woodland. Comparison of the pollen influx values with the theoretical, calculated “characteristic pollen source area” (CPSA) of 12 selected taxa indicates that the pollen deposited on the ground surface of the woodland is a fair representation with 85–90 % of the total pollen deposited at a wind speed of 2.4 m s−1 coming from within ca. 1–5 km for Pinus and Quercus, ca. 5–10 km for Ulmus, Tilia, Oleaceae and Betula, ca. 20–40 km for Fraxinus, Poaceae, Chenopodiaceae, Populus and Salix, and ca. 30–60 km for Artemisia; it is also a good representation with 90–98 % of the total pollen deposited coming from within 60 km at a wind speed of 2.4 m s−1, or 100 km at a wind speed: 6 m s−1, for the 12 selected taxa used in the CPSA calculation. Furthermore, comparison with the vegetation map of the area around the sampling site shows that the pollen deposited on the ground represents all plant communities which grow in the study area within 70 km radius of the sampling site. In this study, the pollen percentages obtained from the soil surface samples are significantly biased towards pollen taxa with good preservation due to thick and robust pollen walls. Therefore, if mosses are available instead, soil samples should be avoided for pollen studies, in particular for the study of pollen-vegetation relationships, the estimation of pollen productivities and quantitative reconstruction of past vegetation. The results also indicate that the existing model of pollen dispersal and deposition, Prentice’s model, provides a fair description of the actual pollen dispersal and deposition in this kind of woodland, which suggests that the application of the landscape reconstruction algorithm would be relevant for reconstruction of this type of woodland in the past. KW - Changbai mountains KW - Mixed coniferous and deciduous broad-leaved woodland KW - Vertical pollen dispersal and deposition KW - Characteristic pollen source area Y1 - 2016 U6 - https://doi.org/10.1007/s00334-015-0532-0 SN - 0939-6314 SN - 1617-6278 VL - 25 SP - 29 EP - 43 PB - Springer CY - New York ER - TY - JOUR A1 - Zhang, Pengfei A1 - Behl, Marc A1 - Peng, Xingzhou A1 - Razzaq, Muhammad Yasar A1 - Lendlein, Andreas T1 - Ultrasonic Cavitation Induced Shape-Memory Effect in Porous Polymer Networks JF - Macromolecular rapid communications N2 - Inspired by the application of ultrasonic cavitation based mechanical force (CMF) to open small channels in natural soft materials (skin or tissue), it is explored whether an artificial polymer network can be created, in which shape-changes can be induced by CMF. This concept comprises an interconnected macroporous rhodium-phosphine (Rh-P) coordination polymer network, in which a CMF can reversibly dissociate the Rh-P microphases. In this way, the ligand exchange of Rh-P coordination bonds in the polymer network is accelerated, resulting in a topological rearrangement of molecular switches. This rearrangement of molecular switches enables the polymer network to release internal tension under ultrasound exposure, resulting in a CMF-induced shape-memory capability. The interconnected macroporous structure with thin pore walls is essential for allowing the CMF to effectively permeate throughout the polymer network. Potential applications of this CMF-induced shape-memory polymer can be mechanosensors or ultrasound controlled switches. Y1 - 2016 U6 - https://doi.org/10.1002/marc.201600439 SN - 1022-1336 SN - 1521-3927 VL - 37 SP - 1897 EP - 1903 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Zerson, Mario A1 - Neumann, Martin A1 - Steyrleuthner, Robert A1 - Neher, Dieter A1 - Magerle, Robert T1 - Surface Structure of Semicrystalline Naphthalene Diimide-Bithiophene Copolymer Films Studied with Atomic Force Microscopy JF - Macromolecules : a publication of the American Chemical Society Y1 - 2016 U6 - https://doi.org/10.1021/acs.macromol.6b00988 SN - 0024-9297 SN - 1520-5835 VL - 49 SP - 6549 EP - 6557 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Zeng, Ting A1 - Frasca, Stefano A1 - Rumschöttel, Jens A1 - Koetz, Joachim A1 - Leimkühler, Silke A1 - Wollenberger, Ursula T1 - Role of Conductive Nanoparticles in the Direct Unmediated Bioelectrocatalysis of Immobilized Sulfite Oxidase JF - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis KW - Direct electron transfer KW - Protein voltammetry KW - Human sulfite oxidase KW - Bioelectrocatalysis KW - Nanoparticles Y1 - 2016 U6 - https://doi.org/10.1002/elan.201600246 SN - 1040-0397 SN - 1521-4109 VL - 28 SP - 2303 EP - 2310 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Zemp, Roland A1 - Fliesser, Michael A1 - Wippert, Pia-Maria A1 - Taylor, William R. A1 - Lorenzetti, Silvio T1 - Occupational sitting behaviour and its relationship with back pain - A pilot study JF - Applied ergonomics : human factors in technology and society N2 - Nowadays, working in an office environment is ubiquitous. At the same time, progressively more people suffer from occupational musculoskeletal disorders. Therefore, the aim of this pilot study was to analyse the influence of back pain on sitting behaviour in the office environment. A textile pressure mat (64-sensor-matrix) placed on the seat pan was used to identify the adopted sitting positions of 20 office workers by means of random forest classification. Additionally, two standardised questionnaires (Korff, BPI) were used to assess short and long-term back pain in order to divide the subjects into two groups (with and without back pain). Independent t-test indicated that subjects who registered back pain within the last 24 h showed a clear trend towards a more static sitting behaviour. Therefore, the developed sensor system has successfully been introduced to characterise and compare sitting behaviour of subjects with and without back pain. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licensesiby-nc-nd/4.0/). KW - Office chair KW - Pressure distribution KW - Musculoskeletal disorders Y1 - 2016 U6 - https://doi.org/10.1016/j.apergo.2016.03.007 SN - 0003-6870 SN - 1872-9126 VL - 56 SP - 84 EP - 91 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Zehbe, Rolf A1 - Zehbe, Kerstin T1 - Strontium doped poly-epsilon-caprolactone composite scaffolds made by reactive foaming JF - The European journal of the history of economic thought N2 - In the reconstruction and regeneration of bone tissue, a primary goal is to initiate bone growth and to stabilize the surrounding bone. In this regard, a potentially useful component in biomaterials for bone tissue engineering is strontium, which acts as cationic active agent, triggering certain intracellular pathways and acting as so called dual action bone agent which inhibits bone resorption while stimulating bone regeneration. In this study we established a novel processing for the foaming of a polymer (poly-epsilon-caprolactone) and simultaneous chemical reaction of a mixture of calcium and strontium hydroxides to the respective carbonates using supercritical carbon dioxide. The resultant porous composite scaffold was optimized in composition and strontium content and was characterized via different spectroscopic (infrared and Raman spectroscopy, energy dispersive X-ray spectroscopy), imaging (SEM, mu CT), mechanical testing and in vitro methods (fluorescence vital staining, MTT-assay). As a result, the composite scaffold showed good in vitro biocompatibility with partly open pore structure and the expected chemistry. First mechanical testing results indicate sufficient mechanical stability to support future in vivo applications. (C) 2016 Elsevier B.V. All rights reserved. KW - Strontium KW - Poly-epsilon-caprolactone KW - Porous scaffold KW - CAL-72 osteoblasts KW - L-929 fibroblasts KW - Reactive foaming KW - mu CT imaging KW - Spectroscopy Y1 - 2016 U6 - https://doi.org/10.1016/j.msec.2016.05.045 SN - 0928-4931 SN - 1873-0191 VL - 67 SP - 259 EP - 266 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Zehbe, Rolf A1 - Zaslansky, Paul A1 - Mochales, Carolina A1 - Mueller, Wolf-Dieter A1 - Fleck, Claudia T1 - Synchrotron micro tomographic evaluation of multilayered zirconia ceramics-Volumetric effects after indentation JF - Journal of the European Ceramic Society N2 - Electrophoretic deposition was used to produce zirconia specimen consisting of alternating layers of fully stabilized cubic zirconia and partially stabilized tetragonal zirconia. In this configuration, the tetragonal stabilized zirconia layers can undergo transformation toughening upon mechanical induced stresses, while the cubic stabilized layers can act as confining element. To understand the volumetric changes due to transformation toughening in these layered materials after indentation, we used an advanced synchrotron-based X-ray mu CT setup and compared the results with surface sensitive methods like Raman spectroscopy, AFM and white light interferometry. The high spatial resolution and the adapted beam energy between the absorption edges of zirconia and yttria allowed discriminating between individual layers due to differences in their yttria content. Furthermore we were able to identify single indents and link volume changes to different physical effects in the different stabilized zirconia parts and visualize the three dimensional volume around only few micrometre sized indents. (C) 2015 Elsevier Ltd. All rights reserved. KW - Yttria stabilized zirconia multilayers KW - X-ray mu CT KW - Electrophoretic deposition KW - Transformation toughening Y1 - 2016 U6 - https://doi.org/10.1016/j.jeurceramsoc.2015.09.015 SN - 0955-2219 SN - 1873-619X VL - 36 SP - 171 EP - 177 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Zehbe, Rolf A1 - Mochales, Carolina A1 - Radzik, Daniela A1 - Mueller, Wolf-Dieter A1 - Fleck, Claudia T1 - Electrophoretic deposition of multilayered (cubic and tetragonal stabilized) zirconia ceramics for adapted crack deflection JF - Journal of the European Ceramic Society N2 - The electrophoretic deposition process was used to produce multi-layered ceramics consisting of alternating layers of fully stabilized cubic zirconia and partially stabilized tetragonal zirconia to make use of their different mechanical behaviour, investigating the possibility to deflect advancing cracks at the interfaces of the different layers. This crack deflection is apparently impacted by a toughening mechanism only found in the tetragonal stabilized zirconia polymorph and is characterized by the stress induced transformation of the metastable tetragonal phase into the monoclinic one, which is accompanied by a volume increase resulting in a closing mechanism for advancing cracks. While improving the electrophoretic deposition process, we investigated the transformation toughening mechanism at the layer interfaces and their effect on crack propagation. Investigations involved a combination of different imaging methods, including light microscopy, white light interferometry, atomic force microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and Raman spectroscopy. (C) 2015 Elsevier Ltd. All rights reserved. KW - Electrophoretic deposition KW - Yttria stabilized zirconia KW - Transformation toughening KW - Multilayer KW - Crack deflection Y1 - 2016 U6 - https://doi.org/10.1016/j.jeurceramsoc.2015.08.022 SN - 0955-2219 SN - 1873-619X VL - 36 SP - 357 EP - 364 PB - Elsevier CY - Oxford ER - TY - JOUR A1 - Zehbe, Kerstin A1 - Kollosche, Matthias A1 - Lardong, Sebastian A1 - Kelling, Alexandra A1 - Schilde, Uwe A1 - Taubert, Andreas T1 - Ionogels Based on Poly(methyl methacrylate) and Metal-Containing Ionic Liquids: Correlation between Structure and Mechanical and Electrical Properties JF - International journal of molecular sciences N2 - Ionogels (IGs) based on poly(methyl methacrylate) (PMMA) and the metal-containing ionic liquids (ILs) bis-1-butyl-3-methlimidazolium tetrachloridocuprate(II), tetrachloride cobaltate(II), and tetrachlorido manganate(II) have been synthesized and their mechanical and electrical properties have been correlated with their microstructure. Unlike many previous examples, the current IGs show a decreasing stability in stress-strain experiments on increasing IL fractions. The conductivities of the current IGs are lower than those observed in similar examples in the literature. Both effects are caused by a two-phase structure with micrometer-sized IL-rich domains homogeneously dispersed an IL-deficient continuous PMMA phase. This study demonstrates that the IL-polymer miscibility and the morphology of the IGs are key parameters to control the (macroscopic) properties of IGs. KW - microstructure KW - ionogels KW - ionic liquids KW - phase separation KW - mechanical properties KW - ionic conductivity Y1 - 2016 U6 - https://doi.org/10.3390/ijms17030391 SN - 1422-0067 VL - 17 PB - MDPI CY - Basel ER - TY - JOUR A1 - Zancolli, Giulia A1 - Baker, Timothy G. A1 - Barlow, Axel A1 - Bradley, Rebecca K. A1 - Calvete, Juan J. A1 - Carter, Kimberley C. A1 - de Jager, Kaylah A1 - Owens, John Benjamin A1 - Price, Jenny Forrester A1 - Sanz, Libia A1 - Scholes-Higham, Amy A1 - Shier, Liam A1 - Wood, Liam A1 - Wüster, Catharine E. A1 - Wüster, Wolfgang T1 - Is Hybridization a Source of Adaptive Venom Variation in Rattlesnakes? A Test, Using a Crotalus scutulatus x viridis Hybrid Zone in Southwestern New Mexico JF - Toxins N2 - Venomous snakes often display extensive variation in venom composition both between and within species. However, the mechanisms underlying the distribution of different toxins and venom types among populations and taxa remain insufficiently known. Rattlesnakes (Crotalus, Sistrurus) display extreme inter-and intraspecific variation in venom composition, centered particularly on the presence or absence of presynaptically neurotoxic phospholipases A2 such as Mojave toxin (MTX). Interspecific hybridization has been invoked as a mechanism to explain the distribution of these toxins across rattlesnakes, with the implicit assumption that they are adaptively advantageous. Here, we test the potential of adaptive hybridization as a mechanism for venom evolution by assessing the distribution of genes encoding the acidic and basic subunits of Mojave toxin across a hybrid zone between MTX-positive Crotalus scutulatus and MTX-negative C. viridis in southwestern New Mexico, USA. Analyses of morphology, mitochondrial and single copy-nuclear genes document extensive admixture within a narrow hybrid zone. The genes encoding the two MTX subunits are strictly linked, and found in most hybrids and backcrossed individuals, but not in C. viridis away from the hybrid zone. Presence of the genes is invariably associated with presence of the corresponding toxin in the venom. We conclude that introgression of highly lethal neurotoxins through hybridization is not necessarily favored by natural selection in rattlesnakes, and that even extensive hybridization may not lead to introgression of these genes into another species. KW - adaptation KW - Crotalus KW - evolution KW - hybridization KW - introgression KW - Mojave toxin KW - molecular evolution KW - venom Y1 - 2016 U6 - https://doi.org/10.3390/toxins8060188 SN - 2072-6651 VL - 8 PB - MDPI CY - Basel ER - TY - JOUR A1 - Zaks, Michael A. A1 - Tomov, Petar T1 - Onset of time dependence in ensembles of excitable elements with global repulsive coupling JF - Physical review : E, Statistical, nonlinear and soft matter physics N2 - We consider the effect of global repulsive coupling on an ensemble of identical excitable elements. An increase of the coupling strength destabilizes the synchronous equilibrium and replaces it with many attracting oscillatory states, created in the transcritical heteroclinic bifurcation. The period of oscillations is inversely proportional to the distance from the critical parameter value. If the elements interact with the global field via the first Fourier harmonics of their phases, the stable equilibrium is in one step replaced by the attracting continuum of periodic motions. Y1 - 2016 U6 - https://doi.org/10.1103/PhysRevE.93.020201 SN - 2470-0045 SN - 2470-0053 VL - 93 PB - American Physical Society CY - College Park ER - TY - JOUR A1 - Zaklan, Aleksandar A1 - Abrell, Jan A1 - Neumann, Anne T1 - Stationarity changes in long-run energy commodity prices JF - Energy economics N2 - Situated at the intersection of the literatures on speculative storage and non-renewable commodity scarcity, this paper considers whether changes in persistence have occurred in long-run U.S. prices of the energy commodities crude oil, natural gas and bituminous coal. We allow for a structural break when testing for a break in persistence to avoid a change in the stochastic properties of prices being confounded by an unaccounted-for deterministic shift in the price series. We find that coal prices are trend stationary throughout their evolution and that oil prices change from stationarity to non-stationarity in the decade between the late 1960s to late 1970s. The result on gas prices is ambiguous. Our results demonstrate the importance of accounting for a possible structural shift when testing for breaks in persistence, while being robust to the exact date of the structural break. Based on our analysis we caution against viewing long-run energy commodity prices as being non-stationary and conclude in favor of modeling commodity market fundamentals as stationary, meaning that speculative storage will tend to have a dampening effect on prices. We also cannot reject that long-run prices of coal and, with some hesitation, gas follow a Hotelling-type rule. In contrast, we reject the Hotelling rule for oil prices since the late 1960s/early 1970s. (C) 2016 Elsevier B.V. All rights reserved. KW - Non-renewable commodity prices KW - Competitive storage KW - Resource scarcity KW - Stationarity KW - Structural breaks Y1 - 2016 U6 - https://doi.org/10.1016/j.eneco.2016.07.022 SN - 0140-9883 SN - 1873-6181 VL - 59 SP - 96 EP - 103 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Zakariás, Lilla A1 - Keresztes, Attila A1 - Marton, Klara A1 - Wartenburger, Isabell T1 - Positive effects of a computerised working memory and executive function JF - Neuropsychological rehabilitation N2 - Aphasia, the language disorder following brain damage, is frequently accompanied by deficits of working memory (WM) and executive functions (EFs). Recent studies suggest that WM, together with certain EFs, can play a role in sentence comprehension in individuals with aphasia (IWA), and that WM can be enhanced with intensive practice. Our aim was to investigate whether a combined WM and EF training improves the understanding of spoken sentences in IWA. We used a pre-post-test case control design. Three individuals with chronic aphasia practised an adaptive training task (a modified n-back task) three to four times a week for a month. Their performance was assessed before and after the training on outcome measures related to WM and spoken sentence comprehension. One participant showed significant improvement on the training task, another showed a tendency for improvement, and both of them improved significantly in spoken sentence comprehension. The third participant did not improve on the training task, however, she showed improvement on one measure of spoken sentence comprehension. Compared to controls, two individuals improved at least in one condition of the WM outcome measures. Thus, our results suggest that a combined WM and EF training can be beneficial for IWA. KW - Aphasia rehabilitation KW - transfer effect KW - updating training KW - interference control KW - sentence comprehension deficit Y1 - 2016 U6 - https://doi.org/10.1080/09602011.2016.1159579 SN - 0960-2011 SN - 1464-0694 VL - 28 IS - 3 SP - 369 EP - 386 PB - Routledge, Taylor & Francis Group CY - Abingdon ER - TY - JOUR A1 - Zabel, André A1 - Winter, Alette A1 - Kelling, Alexandra A1 - Schilde, Uwe A1 - Strauch, Peter T1 - Tetrabromidocuprates(II)-Synthesis, Structure and EPR JF - International journal of molecular sciences N2 - Metal-containing ionic liquids (ILs) are of interest for a variety of technical applications, e.g., particle synthesis and materials with magnetic or thermochromic properties. In this paper we report the synthesis of, and two structures for, some new tetrabromidocuprates(II) with several “onium” cations in comparison to the results of electron paramagnetic resonance (EPR) spectroscopic analyses. The sterically demanding cations were used to separate the paramagnetic Cu(II) ions for EPR measurements. The EPR hyperfine structure in the spectra of these new compounds is not resolved, due to the line broadening resulting from magnetic exchange between the still-incomplete separated paramagnetic Cu(II) centres. For the majority of compounds, the principal g values (g|| and gK) of the tensors could be determined and information on the structural changes in the [CuBr4]2- anions can be obtained. The complexes have high potential, e.g., as ionic liquids, as precursors for the synthesis of copper bromide particles, as catalytically active or paramagnetic ionic liquids. KW - tetrabromidocuprate(II) KW - X-ray structure KW - electron paramagnetic resonance KW - copper(II) Y1 - 2016 U6 - https://doi.org/10.3390/ijms17040596 VL - 17 IS - 4 PB - MDPI CY - Basel ER - TY - JOUR A1 - Zabel, Andre A1 - Winter, Alette A1 - Kelling, Alexandra A1 - Schilde, Uwe A1 - Strauch, Peter T1 - Tetrabromidocuprates(II)-Synthesis, Structure and EPR JF - International journal of molecular sciences N2 - Metal-containing ionic liquids (ILs) are of interest for a variety of technical applications, e.g., particle synthesis and materials with magnetic or thermochromic properties. In this paper we report the synthesis of, and two structures for, some new tetrabromidocuprates(II) with several "onium" cations in comparison to the results of electron paramagnetic resonance (EPR) spectroscopic analyses. The sterically demanding cations were used to separate the paramagnetic Cu(II) ions for EPR measurements. The EPR hyperfine structure in the spectra of these new compounds is not resolved, due to the line broadening resulting from magnetic exchange between the still-incomplete separated paramagnetic Cu(II) centres. For the majority of compounds, the principal g values (g|| and g(perpendicular to)) of the tensors could be determined and information on the structural changes in the [CuBr4](2-) anions can be obtained. The complexes have high potential, e.g., as ionic liquids, as precursors for the synthesis of copper bromide particles, as catalytically active or paramagnetic ionic liquids. KW - tetrabromidocuprate(II) KW - X-ray structure KW - electron paramagnetic resonance KW - copper(II) Y1 - 2016 U6 - https://doi.org/10.3390/ijms17040596 SN - 1422-0067 VL - 17 PB - MDPI CY - Basel ER - TY - JOUR A1 - Yepes, Hugo A1 - Audin, Laurence A1 - Alvarado, Alexandra A1 - Beauval, Celine A1 - Aguilar, Jorge A1 - Font, Yvonne A1 - Cotton, Fabrice Pierre T1 - A new view for the geodynamics of Ecuador: Implication in seismogenic source definition and seismic hazard assessment JF - Tectonics N2 - A new view of Ecuador's complex geodynamics has been developed in the course of modeling seismic source zones for probabilistic seismic hazard analysis. This study focuses on two aspects of the plates' interaction at a continental scale: (a) age-related differences in rheology between Farallon and Nazca plates—marked by the Grijalva rifted margin and its inland projection—as they subduct underneath central Ecuador, and (b) the rapidly changing convergence obliquity resulting from the convex shape of the South American northwestern continental margin. Both conditions satisfactorily explain several characteristics of the observed seismicity and of the interseismic coupling. Intermediate-depth seismicity reveals a severe flexure in the Farallon slab as it dips and contorts at depth, originating the El Puyo seismic cluster. The two slabs position and geometry below continental Ecuador also correlate with surface expressions observable in the local and regional geology and tectonics. The interseismic coupling is weak and shallow south of the Grijalva rifted margin and increases northward, with a heterogeneous pattern locally associated to the Carnegie ridge subduction. High convergence obliquity is responsible for the North Andean Block northeastward movement along localized fault systems. The Cosanga and Pallatanga fault segments of the North Andean Block-South American boundary concentrate most of the seismic moment release in continental Ecuador. Other inner block faults located along the western border of the inter-Andean Depression also show a high rate of moderate-size earthquake production. Finally, a total of 19 seismic source zones were modeled in accordance with the proposed geodynamic and neotectonic scheme. Y1 - 2016 U6 - https://doi.org/10.1002/2015TC003941 SN - 0278-7407 SN - 1944-9194 VL - 35 SP - 1249 EP - 1279 PB - American Geophysical Union CY - Washington ER - TY - JOUR A1 - Yarman, Aysu A1 - Scheller, Frieder W. T1 - MIP-esterase/Tyrosinase Combinations for Paracetamol and Phenacetin JF - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis N2 - A new electrochemical MIP sensor for the most frequently used drug paracetamol (PAR) was prepared by electropolymerization of mixtures containing the template molecule and the functional monomers ophenylenediamine, resorcinol and aniline. The imprinting factor of 12 reflects the effective target binding to the MIP as compared with the non-imprinted electropolymer. Combination of the MIP with a nonspecific esterase allows the measurement of phenacetin - another analgesic drug. In the second approach the PAR containing sample solution was pretreated with tyrosinase in order to prevent electrochemical interferences by ascorbic acid and uric acid. Interference-free indication at a very low electrode potential without fouling of the electrode surface was achieved with the o-phenylenediamine: resorcinol-based MIP. KW - Paracetamol KW - Molecularly imprinted polymers KW - Electropolymerization KW - Tyrosinase KW - Esterase KW - Phenacetin Y1 - 2016 U6 - https://doi.org/10.1002/elan.201600042 SN - 1040-0397 SN - 1521-4109 VL - 28 SP - 2222 EP - 2227 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Yang, Jie A1 - Gühr, Markus A1 - Vecchione, Theodore A1 - Robinson, Matthew Scott A1 - Li, Renkai A1 - Hartmann, Nick A1 - Shen, Xiaozhe A1 - Coffee, Ryan A1 - Corbett, Jeff A1 - Fry, Alan A1 - Gaffney, Kelly A1 - Gorkhover, Tais A1 - Hast, Carsten A1 - Jobe, Keith A1 - Makasyuk, Igor A1 - Reid, Alexander A1 - Robinson, Joseph A1 - Vetter, Sharon A1 - Wang, Fenglin A1 - Weathersby, Stephen A1 - Yoneda, Charles A1 - Wang, Xijie A1 - Centurion, Martin T1 - Femtosecond gas phase electron diffraction with MeV electrons JF - Faraday discussions N2 - We present results on ultrafast gas electron diffraction (UGED) experiments with femtosecond resolution using the MeV electron gun at SLAC National Accelerator Laboratory. UGED is a promising method to investigate molecular dynamics in the gas phase because electron pulses can probe the structure with a high spatial resolution. Until recently, however, it was not possible for UGED to reach the relevant timescale for the motion of the nuclei during a molecular reaction. Using MeV electron pulses has allowed us to overcome the main challenges in reaching femtosecond resolution, namely delivering short electron pulses on a gas target, overcoming the effect of velocity mismatch between pump laser pulses and the probe electron pulses, and maintaining a low timing jitter. At electron kinetic energies above 3 MeV, the velocity mismatch between laser and electron pulses becomes negligible. The relativistic electrons are also less susceptible to temporal broadening due to the Coulomb force. One of the challenges of diffraction with relativistic electrons is that the small de Broglie wavelength results in very small diffraction angles. In this paper we describe the new setup and its characterization, including capturing static diffraction patterns of molecules in the gas phase, finding time-zero with sub-picosecond accuracy and first time-resolved diffraction experiments. The new device can achieve a temporal resolution of 100 fs root-mean-square, and sub-angstrom spatial resolution. The collimation of the beam is sufficient to measure the diffraction pattern, and the transverse coherence is on the order of 2 nm. Currently, the temporal resolution is limited both by the pulse duration of the electron pulse on target and by the timing jitter, while the spatial resolution is limited by the average electron beam current and the signal-to-noise ratio of the detection system. We also discuss plans for improving both the temporal resolution and the spatial resolution. Y1 - 2016 U6 - https://doi.org/10.1039/c6fd00071a SN - 1359-6640 SN - 1364-5498 VL - 194 SP - 563 EP - 581 PB - Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Yang, Jie A1 - Gühr, Markus A1 - Vecchione, Theodore A1 - Robinson, Matthew Scott A1 - Li, Renkai A1 - Hartmann, Nick A1 - Shen, Xiaozhe A1 - Coffee, Ryan A1 - Corbett, Jeff A1 - Fry, Alan A1 - Gaffney, Kelly A1 - Gorkhover, Tais A1 - Hast, Carsten A1 - Jobe, Keith A1 - Makasyuk, Igor A1 - Reid, Alexander A1 - Robinson, Joseph A1 - Vetter, Sharon A1 - Wang, Fenglin A1 - Weathersby, Stephen A1 - Yoneda, Charles A1 - Centurion, Martin A1 - Wang, Xijie T1 - Diffractive imaging of a rotational wavepacket in nitrogen molecules with femtosecond megaelectronvolt electron pulses JF - Nature Communications N2 - Imaging changes in molecular geometries on their natural femtosecond timescale with sub-Angstrom spatial precision is one of the critical challenges in the chemical sciences, as the nuclear geometry changes determine the molecular reactivity. For photoexcited molecules, the nuclear dynamics determine the photoenergy conversion path and efficiency. Here we report a gas-phase electron diffraction experiment using megaelectronvolt (MeV) electrons, where we captured the rotational wavepacket dynamics of nonadiabatically laser-aligned nitrogen molecules. We achieved a combination of 100 fs root-mean-squared temporal resolution and sub-Angstrom (0.76 angstrom) spatial resolution that makes it possible to resolve the position of the nuclei within the molecule. In addition, the diffraction patterns reveal the angular distribution of the molecules, which changes from prolate (aligned) to oblate (anti-aligned) in 300 fs. Our results demonstrate a significant and promising step towards making atomically resolved movies of molecular reactions. Y1 - 2016 U6 - https://doi.org/10.1038/ncomms11232 SN - 2041-1723 VL - 7 PB - Nature Publ. Group CY - London ER -