TY  - JOUR
A1  - Fallah, Mohammad A.
A1  - Stanglmair, Christoph
A1  - Pacholski, Claudia
A1  - Hauser, Karin
T1  - Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-L-lysine Conformational Changes
JF  - Langmuir
N2  - Surface-enhanced infrared absorption spectroscopy (SEIRA) is applied to study protein conformational changes. In general, the appropriate functionalization of metal surfaces with biomolecules remains a challenge if the conformation and activity of the biomolecule shall be preserved. Here we present a SEIRA study to monitor pH-induced conformational changes of poly-L lysine (PLL) covalently bound to a thin gold layer via self assembled monolayers (SAMs). We demonstrate that the composition of the SAM is crucial. A SAM of 11-mercaptoundecanonic acid (MUA) can link PLL to the gold layer, but pH-driven conformational transitions were hindered compared to poly-L lysine in solution. To address this problem, we devised a variety of SAMs, i.e., mixed SAMs of MUA with either octanethiol (OT) or 11-mercapto-1-undecanol (MUoL) and furthermore SAMs of MT(PEG)(4) and NHS-PEG(10k)-SH. These mixed SAMs modify the surface properties by changing the polarity and the morphology of the surface present to nearby PLL molecules. Our experiments reveal that mixed SAMs of MUA-MUoL and SAMs of NHS-PEG(10k)-SH-MT(PEG)(4) are suitable to monitor pH-driven conformational changes of immobilized PLL. These SAMs might be applicable for chemoselective protein immobilization in general.
Y1  - 2016
U6  - https://doi.org/10.1021/acs.langmuir.6b01742
SN  - 0743-7463
VL  - 32
SP  - 7356
EP  - 7364
PB  - American Chemical Society
CY  - Washington
ER  -