TY - GEN A1 - Albers, Philip A1 - Uestuen, Suayib A1 - Witzel, Katja A1 - Bornke, Frederik T1 - Identification of a novel target of the bacterial effector HopZ1a T2 - Phytopathology N2 - The plant pathogen Pseudomonas syringae is a gram-negative bacterium which infects a wide range of plant species including important crops plants. To suppress plant immunity and cause disease P.syringae injects type-III effector proteins (T3Es) into the plant cell cytosol. In this study, we identified a novel target of the well characterized bacterial T3E HopZ1a. HopZ1a is an acetyltransferase that was shown to disrupt vesicle transport during innate immunity by acetylating tubulin. Using a yeast-two-hybrid screen approach, we identified a REMORIN (REM) protein from tobacco as a novel HopZ1a target. HopZ1a interacts with REM at the plasma membrane (PM) as shown by split-YFP experiments. Interestingly, we found that PBS1, a well-known kinase involved in plant immunity also interacts with REM in pull-down assays, and at the PM as shown by BiFC. Furthermore, we confirmed that REM is phosphorylated by PBS1 in vitro. Overexpression of REM provokes the upregulation of defense genes and leads to disease-like phenotypes pointing to a role of REM in plant immune signaling. Further protein-protein interaction studies reveal novel REM binding partners with a possible role in plant immune signaling. Thus, REM might act as an assembly hub for an immune signaling complex targeted by HopZ1a. Taken together, this is the first report describing that a REM protein is targeted by a bacterial effector. How HopZ1a might mechanistically manipulate the plant immune system through interfering with REM function will be discussed. Y1 - 2018 SN - 0031-949X SN - 1943-7684 VL - 108 IS - 10 PB - American Phytopathological Society CY - Saint Paul ER - TY - GEN A1 - Alirezaeizanjani, Zahra A1 - Waljor, V. A1 - Hintsche, Marius A1 - Beta, Carsten T1 - How growth conditions affect bacterial chemotaxis responses T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2017 SN - 0175-7571 SN - 1432-1017 VL - 46 SP - S281 EP - S281 PB - Springer CY - New York ER - TY - GEN A1 - Arnold, Patrick T1 - The origin of morphological integration and modularity in the Mammalian Neck T2 - Journal of morphology Y1 - 2019 U6 - https://doi.org/10.1002/jmor.21003 SN - 0362-2525 SN - 1097-4687 VL - 280 SP - S13 EP - S13 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Autenrieth, Marijke A1 - Ernst, Anja A1 - Deaville, Rob A1 - Demaret, Fabien A1 - Ijsseldijk, Lonneke L. A1 - Siebert, Ursula A1 - Tiedemann, Ralph T1 - Putative origin and maternal relatedness of male sperm whales (Physeter macrocephalus) recently stranded in the North Sea T2 - Mammalian biology = Zeitschrift für Säugetierkunde N2 - The globally distributed sperm whale (Physeter macrocephalus) has a partly matrilineal social structure with predominant male dispersal. At the beginning of 2016, a total of 30 male sperm whales stranded in five different countries bordering the southern North Sea. It has been postulated that these individuals were on a migration route from the north to warmer temperate and tropical waters where females live in social groups. By including samples from four countries (n = 27), this event provided a unique chance to genetically investigate the maternal relatedness and the putative origin of these temporally and spatially co-occuring male sperm whales. To utilize existing genetic resources, we sequenced 422 bp of the mitochondrial control region, a molecular marker for which sperm whale data are readily available from the entire distribution range. Based on four single nucleotide polymorphisms (SNPs) within the mitochondrial control region, five matrilines could be distinguished within the stranded specimens, four of which matched published haplotypes previously described in the Atlantic. Among these male sperm whales, multiple matrilineal lineages co-occur. We analyzed the population differentiation and could show that the genetic diversity of these male sperm whales is comparable to the genetic diversity in sperm whales from the entire Atlantic Ocean. We confirm that within this stranding event, males do not comprise maternally related individuals and apparently include assemblages of individuals from different geographic regions. (c) 2017 Deutsche Gesellschaft fur Saugetierkunde. Published by Elsevier GmbH. All rights reserved. KW - Mitochondrial DNA KW - Maternal relationships KW - Population genetics KW - Migration KW - Marine mammals Y1 - 2018 U6 - https://doi.org/10.1016/j.mambio.2017.09.003 SN - 1616-5047 SN - 1618-1476 VL - 88 SP - 156 EP - 160 PB - Elsevier CY - München ER - TY - GEN A1 - Balazadeh, Salma A1 - Müller-Röber, Bernd T1 - A balance to death T2 - Nature plants N2 - Leaf senescence plays a crucial role in nutrient recovery in late-stage plant development and requires vast transcriptional reprogramming by transcription factors such as ORESARA1 (ORE1). A proteolytic mechanism is now found to control ORE1 degradation, and thus senescence, during nitrogen starvation. Y1 - 2018 U6 - https://doi.org/10.1038/s41477-018-0279-6 SN - 2055-026X SN - 2055-0278 VL - 4 IS - 11 SP - 863 EP - 864 PB - Nature Publ. Group CY - London ER - TY - GEN A1 - Balk, Maria A1 - Grijpma, Dirk W. A1 - Lendlein, Andreas T1 - Design and processing of advanced functional polymers for medicine T2 - Polymers for advanced technologies Y1 - 2017 U6 - https://doi.org/10.1002/pat.3980 SN - 1042-7147 SN - 1099-1581 VL - 28 SP - 1203 EP - 1205 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Barlow, Axel A1 - Sheng, Gui-Lian A1 - Lai, Xu-Long A1 - Hofreiter, Michael A1 - Paijmans, Johanna L. A. T1 - Once lost, twice found: Combined analysis of ancient giant panda sequences characterises extinct clade T2 - Journal of biogeography Y1 - 2018 U6 - https://doi.org/10.1111/jbi.13486 SN - 0305-0270 SN - 1365-2699 VL - 46 IS - 1 SP - 251 EP - 253 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Bartholomäus, Lisa A1 - Lenhard, Michael T1 - Plant Biology: Learning to Love Yourself T2 - Current biology N2 - In self-incompatible plants the female style rejects self pollen, yet the extent to which the female style in the many self-compatible species can still select between different pollen genotypes and thus bias fertilization success is unclear. A new study identifies the molecular basis for how styles of the self-compatible coyote tobacco bias the fertilization success of pollen genotypes using matching gene expression patterns in a manner analogous to cryptic female choice in animals. Y1 - 2019 U6 - https://doi.org/10.1016/j.cub.2019.06.015 SN - 0960-9822 SN - 1879-0445 VL - 29 IS - 14 SP - R695 EP - R697 PB - Cell Press CY - Cambridge ER - TY - GEN A1 - Best, Robert B. A1 - Zheng, Wenwei A1 - Borgia, Alessandro A1 - Buholzer, Karin A1 - Borgia, Madeleine B. A1 - Hofmann, Hagen A1 - Soranno, Andrea A1 - Nettels, Daniel A1 - Gast, Klaus A1 - Grishaev, Alexander A1 - Schuler, Benjamin T1 - Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water" T2 - Science N2 - Riback et al. (Reports, 13 October 2017, p. 238) used small-angle x-ray scattering (SAXS) experiments to infer a degree of compaction for unfolded proteins in water versus chemical denaturant that is highly consistent with the results from Forster resonance energy transfer (FRET) experiments. There is thus no "contradiction" between the two methods, nor evidence to support their claim that commonly used FRET fluorophores cause protein compaction. Y1 - 2018 U6 - https://doi.org/10.1126/science.aar7101 SN - 0036-8075 SN - 1095-9203 VL - 361 IS - 6405 PB - American Assoc. for the Advancement of Science CY - Washington ER - TY - GEN A1 - Brechun, Katherine E. A1 - Woolley, Andrew A1 - Arndt, Katja Maren T1 - A Bacterial Bandpass Assay for Protein-Protein Interactions T2 - Protein science : a publication of the Protein Society Y1 - 2017 SN - 0961-8368 SN - 1469-896X VL - 26 SP - 198 EP - 198 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Breuer, David A1 - Nowak, Jacqueline A1 - Ivakov, Alexander A1 - Somssich, Marc A1 - Persson, Staffan A1 - Nikoloski, Zoran T1 - System-wide organization of actin cytoskeleton determines organelle transport in hypocotyl plant cells T2 - Proceedings of the National Academy of Sciences of the United States of America Y1 - 2017 U6 - https://doi.org/10.1073/pnas.1712371114 SN - 0027-8424 VL - 114 SP - E6732 EP - E6732 PB - National Acad. of Sciences CY - Washington ER - TY - GEN A1 - Broeker, Nina K. A1 - Barbirz, Stefanie T1 - Not a barrier but a key: How bacteriophages exploit host's O‐antigen as an essential receptor to initiate infection T2 - Molecular microbiology N2 - Tailed bacteriophages specific for Gram‐negative bacteria encounter lipopolysaccharide (LPS) during the first infection steps. Yet, it is not well understood how biochemistry of these initial interactions relates to subsequent events that orchestrate phage adsorption and tail rearrangements to initiate cell entry. For many phages, long O‐antigen chains found on the LPS of smooth bacterial strains serve as essential receptor recognized by their tailspike proteins (TSP). Many TSP are depolymerases and O‐antigen cleavage was described as necessary step for subsequent orientation towards a secondary receptor. However, O‐antigen specific host attachment must not always come along with O‐antigen degradation. In this issue of Molecular Microbiology Prokhorov et al. report that coliphage G7C carries a TSP that deacetylates O‐antigen but does not degrade it, whereas rough strains or strains lacking O‐antigen acetylation remain unaffected. Bacteriophage G7C specifically functionalizes its tail by attaching the deacetylase TSP directly to a second TSP that is nonfunctional on the host's O‐antigen. This challenges the view that bacteriophages use their TSP only to clear their way to a secondary receptor. Rather, O‐antigen specific phages may employ enzymatically active TSP as a tool for irreversible LPS membrane binding to initiate subsequent infection steps. Y1 - 2017 U6 - https://doi.org/10.1111/mmi.13729 SN - 0950-382X SN - 1365-2958 VL - 105 SP - 353 EP - 357 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Börnke, Frederik T1 - The complex becomes more complex: protein-protein interactions of SnRK1 with DUF581 family proteins provide a framework for cell- and stimulus type-specific SnRK1 signaling in plants (vol 5, 54, 2014) T2 - Frontiers in plant science Y1 - 2014 U6 - https://doi.org/10.3389/fpls.2014.00693 SN - 1664-462X VL - 5 PB - Frontiers Research Foundation CY - Lausanne ER - TY - GEN A1 - Dammhahn, Melanie A1 - Dingemanse, Niels J. A1 - Niemelae, Petri T. A1 - Reale, Denis T1 - Pace-of-life syndromes BT - a framework for the adaptive integration of behaviour, physiology and life history T2 - Behavioral ecology and sociobiology N2 - This introduction to the topical collection on Pace-of-life syndromes: a framework for the adaptive integration of behaviour, physiology, and life history provides an overview of conceptual, theoretical, methodological, and empirical progress in research on pace-of-life syndromes (POLSs) over the last decade. The topical collection has two main goals. First, we briefly describe the history of POLS research and provide a refined definition of POLS that is applicable to various key levels of variation (genetic, individual, population, species). Second, we summarise the main lessons learned from current POLS research included in this topical collection. Based on an assessment of the current state of the theoretical foundations and the empirical support of the POLS hypothesis, we propose (i) conceptual refinements of theory, particularly with respect to the role of ecology in the evolution of (sexual dimorphism in) POLS, and (ii) methodological and statistical approaches to the study of POLS at all major levels of variation. This topical collection further holds (iii) key empirical examples demonstrating how POLS structures may be studied in wild populations of (non) human animals, and (iv) a modelling paper predicting POLS under various ecological conditions. Future POLS research will profit from the development of more explicit theoretical models and stringent empirical tests of model assumptions and predictions, increased focus on how ecology shapes (sex-specific) POLS structures at multiple hierarchical levels, and the usage of appropriate statistical tests and study designs. Significance statement As an introduction to the topical collection, we summarise current conceptual, theoretical, methodological and empirical progress in research on pace-of-life syndromes (POLSs), a framework for the adaptive integration of behaviour, physiology and life history at multiple hierarchical levels of variation (genetic, individual, population, species). Mixed empirical support of POLSs, particularly at the within-species level, calls for an evaluation and refinement of the hypothesis. We provide a refined definition of POLSs facilitating testable predictions. Future research on POLSs will profit from the development of more explicit theoretical models and stringent empirical tests of model assumptions and predictions, increased focus on how ecology shapes (sex-specific) POLSs structures at multiple hierarchical levels and the usage of appropriate statistical tests and study designs. Y1 - 2018 U6 - https://doi.org/10.1007/s00265-018-2473-y SN - 0340-5443 SN - 1432-0762 VL - 72 IS - 3 PB - Springer CY - New York ER - TY - GEN A1 - Das Gupta, Mainak A1 - Roesch, Florian A1 - Hochrein, Lena A1 - Machens, Fabian A1 - Müller-Röber, Bernd T1 - Facilitating Genome Engineering Through RNP-mediated Precise Gene Targeting T2 - In Vitro Cellular & Developmental Biology - Plant Y1 - 2019 SN - 1054-5476 SN - 1475-2689 VL - 55 IS - 4 SP - 481 EP - 481 PB - Springer CY - New York ER - TY - GEN A1 - de Vera, Jean-Pierre Paul A1 - Alawi, Mashal A1 - Backhaus, Theresa A1 - Baque, Mickael A1 - Billi, Daniela A1 - Boettger, Ute A1 - Berger, Thomas A1 - Bohmeier, Maria A1 - Cockell, Charles A1 - Demets, Rene A1 - de la Torre Noetzel, Rosa A1 - Edwards, Howell A1 - Elsaesser, Andreas A1 - Fagliarone, Claudia A1 - Fiedler, Annelie A1 - Foing, Bernard A1 - Foucher, Frederic A1 - Fritz, Jörg A1 - Hanke, Franziska A1 - Herzog, Thomas A1 - Horneck, Gerda A1 - Hübers, Heinz-Wilhelm A1 - Huwe, Björn A1 - Joshi, Jasmin Radha A1 - Kozyrovska, Natalia A1 - Kruchten, Martha A1 - Lasch, Peter A1 - Lee, Natuschka A1 - Leuko, Stefan A1 - Leya, Thomas A1 - Lorek, Andreas A1 - Martinez-Frias, Jesus A1 - Meessen, Joachim A1 - Moritz, Sophie A1 - Moeller, Ralf A1 - Olsson-Francis, Karen A1 - Onofri, Silvano A1 - Ott, Sieglinde A1 - Pacelli, Claudia A1 - Podolich, Olga A1 - Rabbow, Elke A1 - Reitz, Günther A1 - Rettberg, Petra A1 - Reva, Oleg A1 - Rothschild, Lynn A1 - Garcia Sancho, Leo A1 - Schulze-Makuch, Dirk A1 - Selbmann, Laura A1 - Serrano, Paloma A1 - Szewzyk, Ulrich A1 - Verseux, Cyprien A1 - Wadsworth, Jennifer A1 - Wagner, Dirk A1 - Westall, Frances A1 - Wolter, David A1 - Zucconi, Laura T1 - Limits of life and the habitability of Mars BT - the ESA space experiment BIOMEX on the ISS T2 - Astrobiology N2 - BIOMEX (BIOlogy and Mars EXperiment) is an ESA/Roscosmos space exposure experiment housed within the exposure facility EXPOSE-R2 outside the Zvezda module on the International Space Station (ISS). The design of the multiuser facility supports-among others-the BIOMEX investigations into the stability and level of degradation of space-exposed biosignatures such as pigments, secondary metabolites, and cell surfaces in contact with a terrestrial and Mars analog mineral environment. In parallel, analysis on the viability of the investigated organisms has provided relevant data for evaluation of the habitability of Mars, for the limits of life, and for the likelihood of an interplanetary transfer of life (theory of lithopanspermia). In this project, lichens, archaea, bacteria, cyanobacteria, snow/permafrost algae, meristematic black fungi, and bryophytes from alpine and polar habitats were embedded, grown, and cultured on a mixture of martian and lunar regolith analogs or other terrestrial minerals. The organisms and regolith analogs and terrestrial mineral mixtures were then exposed to space and to simulated Mars-like conditions by way of the EXPOSE-R2 facility. In this special issue, we present the first set of data obtained in reference to our investigation into the habitability of Mars and limits of life. This project was initiated and implemented by the BIOMEX group, an international and interdisciplinary consortium of 30 institutes in 12 countries on 3 continents. Preflight tests for sample selection, results from ground-based simulation experiments, and the space experiments themselves are presented and include a complete overview of the scientific processes required for this space experiment and postflight analysis. The presented BIOMEX concept could be scaled up to future exposure experiments on the Moon and will serve as a pretest in low Earth orbit. KW - EXPOSE-R2 KW - BIOMEX KW - Habitability KW - Limits of life KW - Extremophiles KW - Mars Y1 - 2019 U6 - https://doi.org/10.1089/ast.2018.1897 SN - 1531-1074 SN - 1557-8070 VL - 19 IS - 2 SP - 145 EP - 157 PB - Liebert CY - New Rochelle ER - TY - GEN A1 - Dierschke, Hartmut A1 - Heinken, Thilo T1 - Vorwort T2 - Tuexenia : Mitteilungen der Floristisch-Soziologischen Arbeitsgemeinschaft Y1 - 2019 UR - https://www.tuexenia.de/publications/tuexenia/Tuexenia_2019_NS_039_0007-0007.pdf SN - 0722-494X IS - 39 SP - 7 EP - 7 PB - Floristisch-Soziologische Arbeitsgemeinschaft CY - Göttingen ER - TY - GEN A1 - Dunsing, Valentin A1 - Irmscher, Tobias A1 - Barbirz, Stefanie A1 - Chiantia, Salvatore T1 - Microviscosity of bacterial biofilm matrix characterized by fluorescence correlation spectroscopy and single particle tracking T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2019 U6 - https://doi.org/https://doi.org/10.1007/s00249-019-01373-4 SN - 0175-7571 SN - 1432-1017 VL - 48 SP - S115 EP - S115 PB - Springer CY - New York ER - TY - GEN A1 - Dunsing, Valentin A1 - Magnus, Mayer A1 - Liebsch, Filip A1 - Multhaup, Gerhard A1 - Chiantia, Salvatore T1 - Direct Evidence of APLP1 Trans Interactions in Cell-Cell Adhesion Platforms Investigated via Fluorescence Fluctuation Spectroscopy T2 - Biophysical journal N2 - The Amyloid-precursor-like protein 1 (APLP1) is a neuronal type I transmembrane protein which plays a role in synaptic adhesion and synaptogenesis. Past investigations indicated that APLP1 is involved in the formation of protein-protein complexes that bridge the junctions between neighboring cells. Nevertheless, APLP1-APLP1 trans interactions have never been directly observed in higher eukaryotic cells. Here, we investigate APLP1 interactions and dynamics directly in living human embryonic kidney (HEK) cells, using fluorescence fluctuation spectroscopy techniques, namely cross-correlation scanning fluorescence correlation spectroscopy (sFCS) and Number&Brightness (N&B). Our results show that APLP1 forms homotypic trans complexes at cell-cell contacts. In the presence of zinc ions, the protein forms macroscopic clusters, exhibiting an even higher degree of trans binding and strongly reduced dynamics. Further evidence from Giant Plasma Membrane Vesicles and live cell actin staining suggests that the presence of an intact cortical cytoskeleton is required for zinc-induced cis multimerization. Subsequently, large adhesion platforms bridging interacting cells are formed through APLP1-APLP1 direct trans interactions. Taken together, our results provide direct evidence that APLP1 functions as a neuronal zinc-dependent adhesion protein and provide a more detailed understanding of the molecular mechanisms driving the formation of APLP1 adhesion platforms. Further, they show that fluorescence fluctuation spectroscopy techniques are useful tools for the investigation of protein-protein interactions at cell-cell adhesion sites. Y1 - 2018 U6 - https://doi.org/10.1016/j.bpj.2017.11.2067 SN - 0006-3495 SN - 1542-0086 VL - 114 IS - 3 SP - 373A EP - 373A PB - Cell Press CY - Cambridge ER - TY - GEN A1 - Dunsing, Valentin A1 - Mayer, M. A1 - Multhaup, G. A1 - Chiantia, Salvatore T1 - Direct visualization of APLP1 cell-cell adhesion platforms via fluorescence fluctuation spectroscopy T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2017 SN - 0175-7571 SN - 1432-1017 VL - 46 SP - S374 EP - S374 PB - Springer CY - New York ER - TY - GEN A1 - Ehlert, Britta T1 - Paramutation am SULFUREA-Lokus in Solanum lycopersicum Y1 - 2008 CY - Potsdam ER - TY - GEN A1 - Gonzalez-Fortes, Gloria M. A1 - Tassi, Francesca A1 - Ghirotto, Silvia A1 - Henneberger, Kirstin A1 - Hofreiter, Michael A1 - Barbujani, Guido T1 - The Neolithic transition at the Western edge of Europe T2 - American journal of physical anthropology Y1 - 2017 SN - 0002-9483 SN - 1096-8644 VL - 162 SP - 198 EP - 198 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Gräf, Ralph T1 - Comparative Biology of Centrosomal Structures in Eukaryotes T2 - Cells N2 - The centrosome is not only the largest and most sophisticated protein complex within a eukaryotic cell, in the light of evolution, it is also one of its most ancient organelles. This special issue of "Cells" features representatives of three main, structurally divergent centrosome types, i.e., centriole-containing centrosomes, yeast spindle pole bodies (SPBs), and amoebozoan nucleus-associated bodies (NABs). Here, I discuss their evolution and their key-functions in microtubule organization, mitosis, and cytokinesis. Furthermore, I provide a brief history of centrosome research and highlight recently emerged topics, such as the role of centrioles in ciliogenesis, the relationship of centrosomes and centriolar satellites, the integration of centrosomal structures into the nuclear envelope and the involvement of centrosomal components in non-centrosomal microtubule organization. KW - centrosome KW - centriole KW - cilium KW - basal body KW - spindle pole body KW - SPB KW - nucleus-associated body KW - NAB KW - microtubules Y1 - 2018 U6 - https://doi.org/10.3390/cells7110202 SN - 2073-4409 VL - 7 IS - 11 PB - MDPI CY - Basel ER - TY - GEN A1 - Gzik, Axel T1 - Gefäßversuche zur Beurteilung von Korrelation zwischen Standortfaktoren und vitalitätsbestimmenden Stoffwechselparametern in charakteristischen Pflanzenarten typischer Feuchtwiesengesellschaften. Y1 - 1997 ER - TY - GEN A1 - Hanack, Katja A1 - Schloer, Anja A1 - Holzloehner, Pamela A1 - Listek, Martin A1 - Bauer, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Fuener, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk T1 - Camelid nanobodies specific to human pancreatic glycoprotein 2 T2 - The journal of immunology N2 - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease. Y1 - 2016 SN - 0022-1767 SN - 1550-6606 VL - 196 SP - 313 EP - 328 PB - American Assoc. of Immunologists CY - Bethesda ER - TY - GEN A1 - Hermanussen, Michael A1 - Bogin, Barry A1 - Scheffler, Christiane T1 - The impact of social identity and social dominance on the regulation of human growth: A viewpoint T2 - Acta paediatrica : nurturing the child Y1 - 2019 U6 - https://doi.org/10.1111/apa.14970 SN - 0803-5253 SN - 1651-2227 VL - 108 IS - 12 SP - 2132 EP - 2134 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Hermanussen, Michael A1 - Ipsen, Josefin A1 - Mumm, Rebekka A1 - Assmann, Christian A1 - Quitmann, Julia A1 - Gomula, Aleksandra A1 - Lehmann, Andreas A1 - Jasch, Isabelle A1 - Tassenaar, Vincent A1 - Bogin, Barry A1 - Satake, Takashi A1 - Scheffler, Christiane A1 - Nunez, Javier A1 - Godina, Elena A1 - Hardeland, Ruediger A1 - Boldsen, Jesper L. A1 - El-Shabrawi, Mortada A1 - Elhusseini, Mona A1 - Barbu, Carmen Gabriela A1 - Pop, Ralucca A1 - Soederhaell, Jani A1 - Merker, Andrea A1 - Swanson, James A1 - Groth, Detlef T1 - Stunted Growth. Proceedings of the 23rd Aschauer Soiree, Held at Aschauhof, Germany, November 7th 2015 T2 - Pediatric Endocrinology Reviews N2 - Twenty-four scientists met at Aschauhof, Altenhof, Germany, to discuss the associations between child growth and development, and nutrition, health, environment and psychology. Meta-analyses of body height, height variability and household inequality, in historic and modern growth studies published since 1794, highlighting the enormously flexible patterns of child and adolescent height and weight increments throughout history which do not only depend on genetics, prenatal development, nutrition, health, and economic circumstances, but reflect social interactions. A Quality of Life in Short Stature Youth Questionnaire was presented to cross-culturally assess health-related quality of life in children. Changes of child body proportions in recent history, the relation between height and longevity in historic Dutch samples and also measures of body height in skeletal remains belonged to the topics of this meeting. Bayesian approaches and Monte Carlo simulations offer new statistical tools for the study of human growth. KW - Adolescent growth KW - Peer group KW - Growth hormone KW - Community effect KW - Body height Y1 - 2016 SN - 1565-4753 VL - 13 SP - 756 EP - 767 PB - Medical Media CY - Netanya ER - TY - GEN A1 - Hermanussen, Michael A1 - Scheffler, Christiane A1 - Groth, Detlef A1 - Bogin, Barry T1 - Student work on trends in infant and child growth BT - an editorial T2 - Journal of biological and clinical anthropology : Anthropologischer Anzeiger : Mitteilungsorgan der Gesellschaft für Anthropologie KW - nutrition KW - impact on growth KW - geographic neighborhood KW - mortality bias KW - limb disproportions KW - physical activity KW - socioeconomic status KW - parental age KW - statistical tools Y1 - 2019 U6 - https://doi.org/10.1127/anthranz/2019/1052 SN - 0003-5548 VL - 76 IS - 5 SP - 363 EP - 364 PB - Schweizerbart CY - Stuttgart ER - TY - GEN A1 - Hermanussen, Michael A1 - Scheffler, Christiane A1 - Groth, Detlef A1 - Bogin, Barry T1 - Perceiving stunting - Student research and the "Lieschen Muller effect" in nutrition science T2 - Journal of biological and clinical anthropology : Anthropologischer Anzeiger ; Mitteilungsorgan der Gesellschaft für Anthropologie KW - stunting KW - undernutrition KW - strategic growth adjustments KW - competitive growth KW - community effect on height Y1 - 2018 U6 - https://doi.org/10.1127/anthranz/2018/0858 SN - 0003-5548 VL - 74 IS - 5 SP - 355 EP - 358 PB - Schweizerbart CY - Stuttgart ER - TY - GEN A1 - Hermanussen, Michael A1 - Scheffler, Christiane A1 - Pulungan, Aman B. A1 - Batubara, Jose R. L. A1 - Julia, Madarina A1 - Bogin, Barry T1 - Response to the correspondence referring to our article "Stunting is not a synonym of malnutrition" (2018EJCN0997RR) by Conny Tanjung, Titis Prawitasari, Damayanti Rusli Sjarif T2 - European journal of clinical nutrition Y1 - 2020 U6 - https://doi.org/10.1038/s41430-020-0571-1 SN - 0954-3007 SN - 1476-5640 VL - 74 IS - 3 SP - 529 EP - 531 PB - Nature Publ. Group CY - New York, NY ER - TY - GEN A1 - Higgs, Eric S. A1 - Harris, Jim A. A1 - Heger, Tina A1 - Hobbs, Richard J. A1 - Murphy, Stephen D. A1 - Suding, Katharine N. T1 - Keep ecological restoration open and flexible T2 - Nature Ecology & Evolution Y1 - 2018 U6 - https://doi.org/10.1038/s41559-018-0483-9 SN - 2397-334X VL - 2 IS - 4 SP - 580 EP - 580 PB - Nature Publ. Group CY - London ER - TY - GEN A1 - Holzlöhner, Pamela A1 - Butze, Monique A1 - Hebel, Nicole A1 - Weschke, Daniel A1 - Schliebs, E. A1 - Naumann, F. A1 - Füner, J. A1 - Micheel, Burkhard A1 - Hanack, Katja T1 - Monoclonal mouse antibodies against PBMC subpopulations of New World camelides T2 - European journal of immunology Y1 - 2016 SN - 0014-2980 SN - 1521-4141 VL - 46 SP - 1175 EP - 1175 PB - Wiley-Blackwell CY - Hoboken ER - TY - GEN A1 - Horreo, J. L. A1 - Breedveld, Merel Cathelijne A1 - Lindtke, D. A1 - Heulin, B. A1 - Surget-Groba, Yann A1 - Fitze, Patrick S. T1 - Correction: Genetic introgression among differentiated clades is lower among clades exhibiting different parity modes. - (Heredity. - 123, (2019) pg 264) T2 - Heredity N2 - The original version of this Article contained an error in the spelling of the author Y. Surget-Groba, which was incorrectly given as J. Surget-Groba. This has now been corrected in both the PDF and HTML versions of the Article. Y1 - 2019 U6 - https://doi.org/10.1038/s41437-019-0223-1 SN - 0018-067X SN - 1365-2540 VL - 123 IS - 2 SP - 285 EP - 285 PB - Nature Publ. Group CY - London ER - TY - GEN A1 - Huu, Cuong Nguyen A1 - Plaschil, Sylvia A1 - Himmelbach, Axel A1 - Kappel, Christian A1 - Lenhard, Michael T1 - Female self-incompatibility type in heterostylous Primula is determined by the brassinosteroid-inactivating cytochrome P450 CYP734A50 T2 - Current biology N2 - Most flowering plants are hermaphrodites, with flowers having both male and female reproductive organs. One widespread adaptation to limit self-fertilization is self-incompatibility (SI), where self-pollen fails to fertilize ovules.(1,2) In homomorphic SI, many morphologically indistinguishable mating types are found, although in heteromorphic SI, the two or three mating types are associated with different floral morphologies.(3-6) In heterostylous Primula, a hemizygous supergene determines a short-styled S-morph and a long-styled L-morph, corresponding to two different mating types, and full seed set only results from inter morph crosses.(7-9) Style length is controlled by the brassinosteroid (BR)-inactivating cytochrome P450 CYP734A50,(10) yet it remains unclear what defines the male and female incompatibility types. Here, we show that CYP734A50 also determines the female incompatibility type. Inactivating CYP734A50 converts short S-morph styles into long styles with the same incompatibility behavior as L-morph styles, and this effect can be mimicked by exogenous BR treatment. In vitro responses of S-and L-morph pollen grains and pollen tubes to increasing BR levels could only partly explain their different in vivo behavior, suggesting both direct and indirect effects of the different BR levels in S-versus L-morph stigmas and styles in controlling pollen performance. This BR-mediated SI provides a novel mechanism for preventing self-fertilization. The joint control of morphology and SI by CYP734A50 has important implications for the evolutionary buildup of the heterostylous syndrome and provides a straightforward explanation for why essentially all of the derived self-compatible homostylous Primula species are long homostyles.(11) KW - heteromorphic self-incompatibility KW - heterostyly KW - Primula forbesii KW - brassinosteroid KW - CYP734A50 KW - supergene KW - pleiotropy Y1 - 2022 U6 - https://doi.org/10.1016/j.cub.2021.11.046 SN - 0960-9822 SN - 1879-0445 VL - 32 IS - 3 SP - 671 EP - 676, E1-E5 PB - Cell Press CY - Cambridge, Mass. ER - TY - GEN A1 - Höfer, Chris Tina A1 - Di Lella, Santiago A1 - Dahmani, Ismail A1 - Jungnick, Nadine A1 - Bordag, Natalie A1 - Bobone, Sara A1 - Huan, Q. A1 - Keller, S. A1 - Herrmann, A. A1 - Chiantia, Salvatore T1 - Corrigendum to: Structural determinants of the interaction between influenza A virus matrix protein M1 and lipid membranes (Biochimica et Biophysica Acta (BBA) - Biomembranes. - 1861, (2019), pg 1123-1134) T2 - Biochimica et biophysica acta : Biomembranes Y1 - 2019 U6 - https://doi.org/10.1016/j.bbamem.2019.07.002 SN - 0005-2736 SN - 1879-2642 VL - 1861 IS - 10 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Jeltsch, Florian A1 - Grimm, Volker T1 - Editorial BT - thematic series "Integrating movement ecology with biodiversity research" T2 - Movement Ecology Y1 - 2020 U6 - https://doi.org/10.1186/s40462-020-00210-0 SN - 2051-3933 VL - 8 IS - 1 PB - BioMed Central CY - London ER - TY - GEN A1 - Kalinkat, Gregor A1 - Cabral, Juliano Sarmento A1 - Darwall, William A1 - Ficetola, G. Francesco A1 - Fisher, Judith L. A1 - Giling, Darren P. A1 - Gosselin, Marie-Pierre A1 - Grossart, Hans-Peter A1 - Jaehnig, Sonja C. A1 - Jeschke, Jonathan M. A1 - Knopf, Klaus A1 - Larsen, Stefano A1 - Onandia, Gabriela A1 - Paetzig, Marlene A1 - Saul, Wolf-Christian A1 - Singer, Gabriel A1 - Sperfeld, Erik A1 - Jaric, Ivan T1 - Flagship umbrella species needed for the conservation of overlooked aquatic biodiversity T2 - Conservation biology : the journal of the Society for Conservation Biology Y1 - 2017 U6 - https://doi.org/10.1111/cobi.12813 SN - 0888-8892 SN - 1523-1739 VL - 31 SP - 481 EP - 485 PB - Wiley CY - Hoboken ER - TY - GEN A1 - Kehr, Julia T1 - Untersuchungen der Proteine in den Langstreckentransportsystemen höherer Pflanzen Y1 - 2005 CY - Potsdam ER - TY - GEN A1 - Kleine-Vehn, Jürgen A1 - Sauer, Michael ED - Kleine-Vehn, Jürgen ED - Sauer, Michael T1 - Preface T2 - Plant Hormones: Methods and Protocols Y1 - 2017 SN - 978-1-4939-6469-7 SN - 978-1-4939-6467-3 U6 - https://doi.org/10.1007/978-1-4939-6469-7 SN - 1064-3745 SN - 1940-6029 VL - 1497 SP - V EP - V PB - Springer CY - New York ET - 3 ER - TY - GEN A1 - Knigge, Xenia A1 - Wenger, C. A1 - Bier, Frank Fabian A1 - Hölzel, Ralph T1 - AC electrokinetic immobilisation of nanoobjects as individual singles in regular arrays T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2017 SN - 0175-7571 SN - 1432-1017 VL - 46 SP - S187 EP - S187 PB - Springer CY - New York ER - TY - GEN A1 - Kocyan, Alexander A1 - Wiland-Szymanska, Justyna T1 - A new name and a new combination for Friedmannia nom. illeg. (Hypoxidaceae) T2 - Phytotaxa : a rapid international journal for accelerating the publication of botanical taxonomy N2 - Recently, Kocyan & Wiland-Szymańska (2016) have published a thorough research article on one of the outstanding members of the family Hypoxidaceae on the Seychelles, which resulted in the raise of a new genus (Friedmannia Kocyan & Wiland-Szymańska 2016: 60) to accommodate the former Curculigo seychellensis Bojer ex Baker (1877: 368). However, it has turned out that the name Friedmannia Chantanachat & Bold (1962: 45) already exists in literature for a green alga, which renders the new hypoxid genus illegitimate (Melbourne Code; McNeill et al. 2012). Therefore, we assign a new generic epithet to Curculigo seychellensis. Y1 - 2017 U6 - https://doi.org/10.11646/phytotaxa.291.3.10 SN - 1179-3155 SN - 1179-3163 VL - 291 IS - 3 SP - 239 EP - 239 PB - Magnolia Press CY - Auckland ER - TY - GEN A1 - Koziel, Slawomir A1 - Hermanussen, Michael A1 - Gomula, Alexandra A1 - Swanson, James A1 - Kaczmarek, Maria A1 - El-Shabrawi, Mortada A1 - Elhusseini, Mona A1 - Satake, Takashi A1 - Martinovic Klaric, Irena A1 - Scheffler, Christiane A1 - Morkuniene, Ruta A1 - Godina, Elena A1 - Sasa, Missoni A1 - Tutkuviene, Janina A1 - Siniarska, Anna A1 - Nieczuja-Dwojacka, Joanna A1 - Nunez, Javier A1 - Groth, Detlef A1 - Barbieri, Davide T1 - Adolescence - a Transition to Adulthood Proceedings of the 24th Aschauer Soiree, held at Jurata, Poland, November 5th 2016 T2 - Pediatric Endocrinology Reviews N2 - Eighteen scientists met at Jurata, Poland, to discuss various aspects of the transition from adolescence to adulthood. This transition is a delicate period facing complex interactions between the adolescents and the social group they belong to. Social identity, group identification and identity signalling, but also stress affecting basal salivary cortisol rhythms, hypertension, inappropriate nutrition causing latent and manifest obesity, moreover, in developing and under-developed countries, parasitosis causing anaemia thereby impairing growth and development, are issues to be dealt with during this period of the human development. In addition, some new aspects of the association between weight, height and head circumference in the newborns were discussed, as well as intrauterine head growth and head circumference as health risk indicators. KW - Strategic growth adjustment KW - BMI KW - Growth faltering KW - Secular trend KW - Obesity KW - Growth modelling Y1 - 2017 SN - 1565-4753 VL - 14 IS - 3 SP - 326 EP - 334 PB - Medical Media CY - Netanya ER - TY - GEN A1 - Kramer, Elena M. A1 - Lenhard, Michael T1 - Shape and form in plant development T2 - Seminars in cell & developmental biology Y1 - 2017 U6 - https://doi.org/10.1016/j.semcdb.2017.11.004 SN - 1084-9521 VL - 79 SP - 1 EP - 2 PB - Elsevier CY - London ER - TY - GEN A1 - Laux, Eva-Maria A1 - Docoslis, A. A1 - Wenger, C. A1 - Bier, Frank Fabian A1 - Hölzel, Ralph T1 - Combination of dielectrophoresis and SERS for bacteria detection and characterization T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2017 SN - 0175-7571 SN - 1432-1017 VL - 46 SP - S331 EP - S331 PB - Springer CY - New York ER - TY - GEN A1 - Laux, Eva-Maria A1 - Gibbons, J. A1 - Ermilova, Elena A1 - Bier, Frank Fabian A1 - Hölzel, Ralph T1 - Broadband dielectric spectroscopy of bovine serum albumin in the GHz range T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2017 SN - 0175-7571 SN - 1432-1017 VL - 46 SP - S347 EP - S347 PB - Springer CY - New York ER - TY - GEN A1 - Laux, Eva-Maria A1 - Knigge, Xenia A1 - Wenger, C. A1 - Bier, Frank Fabian A1 - Hölzel, Ralph T1 - AC electrokinetic manipulation of nanoparticles and molecules T2 - European biophysics journal : with biophysics letters ; an international journal of biophysics Y1 - 2017 SN - 0175-7571 SN - 1432-1017 VL - 46 SP - S189 EP - S189 PB - Springer CY - New York ER - TY - GEN A1 - Lechon, Tamara A1 - Sanz, Luis A1 - Pollmann, Stephan A1 - Sauer, Michael A1 - Sandalio, Luisa A1 - Lorenzo, Oscar T1 - Nitric oxide modification of plant endocytosis and PIN1 localization T2 - New biotechnology Y1 - 2016 U6 - https://doi.org/10.1016/j.nbt.2015.10.028 SN - 1871-6784 SN - 1876-4347 VL - 33 SP - 424 EP - 424 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Lenhard, Michael T1 - Plant Development: Keeping on the Straight and Narrow and Flat T2 - Current biology Y1 - 2017 U6 - https://doi.org/10.1016/j.cub.2017.10.030 SN - 0960-9822 SN - 1879-0445 VL - 27 SP - R1277 EP - R1280 PB - Cell Press CY - Cambridge ER - TY - GEN A1 - Lou, Ying A1 - Ma, Hui A1 - Lin, Wen-Hui A1 - Chu, Zhao-Quing A1 - Müller-Röber, Bernd A1 - Xu, Zhi-Hong A1 - Xue, Hong-Wei T1 - The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding N2 - In Arabidopsis thaliana and Oryza sativa, two types of PI 4-kinase (PI4Ks) have been isolated and functionally characterized. The alpha-type PI4Ks (similar to 220 kDa) contain a PH domain, which is lacking in beta-type PI4Ks (similar to 120 kDa). beta-Type PI4Ks, exemplified by Arabidopsis AtPI4K beta and rice OsPI4K2, contain a highly charged repetitive segment designated PPC (Plant PI4K Charged) region, which is an unique domain only found in plant beta-type PI4Ks at present. The PPC region has a length of similar to 300 amino acids and harboring 11 (AtPI4K beta) and 14 (OsPI4K2) repeats, respectively, of a 20-aa motif. Studies employing a modified yeast-based "Sequence of Membrane- Targeting Detection'' system demonstrate that the PPC(OsPI4K2) region, as well as the former 8 and latter 6 repetitive motifs within the PPC region, are able to target fusion proteins to the plasma membrane. Further detection on the transiently expressed GFP fusion proteins in onion epidermal cells showed that the PPC(OsPI4K2) region alone, as well as the region containing repetitive motifs 1-8, was able to direct GFP to the plasma membrane, while the regions containing less repetitive motifs, i.e. 6, 4, 2 or single motif(s) led to predominantly intracellular localization. Agrobacterium-mediated transient expression of PPC-GFP fusion protein further confirms the membrane-targeting capacities of PPC region. In addition, the predominant plasma membrane localization of AtPI4Kb was mediated by the PPC region. Recombinant PPC peptide, expressed in E. coli, strongly binds phosphatidic acid, PI and PI4P, but not phosphatidylcholine, PI5P, or PI(4,5) P-2 in vitro, providing insights into potential mechanisms for regulating sub- cellular localization and lipid binding for the plant beta-type PI4Ks Y1 - 2006 UR - http://www.springerlink.com/content/100330 U6 - https://doi.org/10.1007/s11103-005-5548-x SN - 0167-4412 ER - TY - GEN A1 - Luckner, Madlen A1 - Dunsing, Valentin A1 - Chiantia, Salvatore A1 - Hermann, Andreas T1 - Oligomerization and nuclear shuttling dynamics of viral proteins studied by quantitative molecular brightness analysis using fluorescence correlation spectroscopy T2 - Biophysical journal Y1 - 2018 U6 - https://doi.org/10.1016/j.bpj.2017.11.1951 SN - 0006-3495 SN - 1542-0086 VL - 114 IS - 3 SP - 350A EP - 350A PB - Cell Press CY - Cambridge ER -