TY  - JOUR
A1  - Neumann, Bettina
A1  - Kielb, Patrycja
A1  - Rustam, Lina
A1  - Fischer, Anna
A1  - Weidinger, Inez M.
A1  - Wollenberger, Ulla
T1  - Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase-11 Immobilized on Mesoporous Antimony-Doped Tin Oxide
JF  - ChemElectrChem
N2  - The heme-undecapeptide microperoxidase-11 (MP-11) was immobilized on mesoporous antimony-doped tin oxide (ATO) thin-film electrodes modified with the positively charged binding promotor polydiallyldimethylammonium chloride. Surface concentrations of MP-11 of 1.5 nmol cm(-2) were sufficiently high to enable spectroelectrochemical analyses. UV/Vis spectroscopy and resonance Raman spectroscopy revealed that immobilized MP-11 adopts a six-coordinated low-spin conformation, as in solution in the presence of a polycation. Cathodic reduction of hydrogen peroxide at potentials close to +500mV versus Ag/AgCl indicates that the reaction proceeds via a Compound I-type like intermediate, analogous to natural peroxidases, and confirms mesoporous ATO as a suitable host material for adsorbing the heme-peptide in its native state. A hydrogen peroxide sensor is proposed by using the bioelectrocatalytic properties of the MP-11-modified ATO.
KW  - electrochemistry
KW  - enzyme catalysis
KW  - mesoporous materials
KW  - microperoxidase
KW  - spectroelectrochemistry
Y1  - 2017
U6  - https://doi.org/10.1002/celc.201600776
SN  - 2196-0216
VL  - 4
IS  - 4
SP  - 913
EP  - 919
PB  - Wiley-VCH
CY  - Weinheim
ER  -