TY  - JOUR
A1  - Connor, Daniel Oliver
A1  - Danckert, Lena
A1  - Hoppe, Sebastian
A1  - Bier, Frank Fabian
A1  - von Nickisch-Rosenegk, Markus
T1  - Epitope determination of immunogenic proteins of Neisseria gonorrhoeae
JF  - PLoS one
N2  - Neisseria gonorrhoeae is the causative organism of gonorrhoea, a sexually transmitted disease that globally accounts for an estimated 80 to 100 million new infections per year. Increasing resistances to all common antibiotics used for N. gonorrhoeae treatment pose the risk of an untreatable disease. Further knowledge of ways of infection and host immune response are needed to understand the pathogen-host interaction and to discover new treatment alternatives against this disease. Therefore, detailed information about immunogenic proteins and their properties like epitope sites could advance further research in this area. In this work, we investigated immunogenic proteins of N. gonorrhoeae for linear epitopes by microarrays. Dominant linear epitopes were identified for eleven of the nineteen investigated proteins with three polyclonal rabbit antibodies from different immunisations. Identified linear epitopes were further examined for non-specific binding with antibodies to Escherichia coli and the closely related pathogen Neisseria meningitidis. On top of that, amino acids crucial for the antibody epitope binding were detected by microarray based alanine scans.
Y1  - 2017
U6  - https://doi.org/10.1371/journal.pone.0180962
SN  - 1932-6203
VL  - 12
PB  - PLoS
CY  - San Fransisco
ER  - 
TY  - JOUR
A1  - Fischbach, Jens
A1  - Loh, Qiuting
A1  - Bier, Frank Fabian
A1  - Lim, Theam Soon
A1  - Frohme, Marcus
A1  - Glökler, Jörn
T1  - Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method
JF  - Scientific reports
N2  - We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the presence of manganese as quencher and optimize conditions for an online detection. Of the selected dyes, the inexpensive alizarin red S was found to selectively detect pyrophosphate under LAMP and PCR conditions and is superior with respect to its defined red-shifted spectrum, long shelf life and low toxicity. In addition, the newly identified properties may also be useful in other enzymatic assays which do not generate nucleic acids but are based on inorganic pyrophosphate. Finally, we propose that our screening method may provide a blueprint for rapid screening of compounds for detecting inorganic pyrophosphate.
Y1  - 2017
U6  - https://doi.org/10.1038/srep45085
SN  - 2045-2322
VL  - 7
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - GEN
A1  - Knigge, Xenia
A1  - Wenger, C.
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - AC electrokinetic immobilisation of nanoobjects as individual singles in regular arrays
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S187
EP  - S187
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Laux, Eva-Maria
A1  - Docoslis, A.
A1  - Wenger, C.
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Combination of dielectrophoresis and SERS for bacteria detection and characterization
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S331
EP  - S331
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Laux, Eva-Maria
A1  - Gibbons, J.
A1  - Ermilova, Elena
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Broadband dielectric spectroscopy of bovine serum albumin in the GHz
range
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S347
EP  - S347
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Laux, Eva-Maria
A1  - Knigge, Xenia
A1  - Wenger, C.
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - AC electrokinetic manipulation of nanoparticles and molecules
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S189
EP  - S189
PB  - Springer
CY  - New York
ER  - 
TY  - GEN
A1  - Stanke, S.
A1  - Wenger, C.
A1  - Bier, Frank Fabian
A1  - Hölzel, Ralph
T1  - Dielectrophoretic functionalization of nanoelectrode arrays for the detection of influenza viruses
T2  - European biophysics journal : with biophysics letters ; an international journal of biophysics
Y1  - 2017
SN  - 0175-7571
SN  - 1432-1017
VL  - 46
SP  - S337
EP  - S337
PB  - Springer
CY  - New York
ER  -