TY  - GEN
A1  - Schmälzlin, Elmar
A1  - Walz, Bernd
A1  - Klimant, Ingo
A1  - Schewe, Bettina
A1  - Löhmannsröben, Hans-Gerd
T1  - Monitoring hormone-induced oxygen consumption in the salivary glands of the blowfly, Calliphora vicina, by use of luminescent microbeads
N2  - The salivary glands of the blowfly were injected with luminescent oxygen-sensitive microbeads. The changes in oxygen content within individual gland tubules during hormone-induced secretory activity were quantified. The measurements are based on an upgraded phase-modulation technique, where the phase shift of the sensor phosphorescence is determined independently from concentration and background signals. We show that the combination of a lock-in amplifier with a fluorescence microscope results in a convenient setup to measure oxygen concentrations within living animal tissues at the cellular level.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 006 
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-12206
ER  - 
TY  - GEN
A1  - Schmälzlin, Elmar
A1  - Dongen, Joost T. van
A1  - Klimant, Ingo
A1  - Marmodée, Bettina
A1  - Steup, Martin
A1  - Fishahn, Joachim
A1  - Geigenberger, Peter
A1  - Löhmannsröben, Hans-Gerd
T1  - An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants
N2  - A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 007 
Y1  - 2005
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-12232
ER  -