TY  - GEN
A1  - Prát, Tomáš
A1  - Hajny ́, Jakub
A1  - Grunewald, Wim
A1  - Vasileva, Mina
A1  - Molnár, Gergely
A1  - Tejos, Ricardo
A1  - Schmid, Markus
A1  - Sauer, Michael
A1  - Friml, Jiří
T1  - WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17-and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain-and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1123 
KW  - apical-basal axis
KW  - arabidopsis-thaliana
KW  - root gravitropism
KW  - DNA-binding
KW  - gene-expression
KW  - transport
KW  - efflux
KW  - canalization
KW  - plants
KW  - phosphorylation
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-446331
SN  - 1866-8372
IS  - 1123
ER  -