TY  - JOUR
A1  - Sagu Tchewonpi, Sorel
A1  - Landgräber, Eva
A1  - Rackiewicz, Michal
A1  - Huschek, Gerd
A1  - Rawel, Harshadrai Manilal
T1  - Relative Abundance of Alpha-Amylase/Trypsin Inhibitors in Selected Sorghum Cultivars
JF  - Molecules
N2  - Sorghum is of growing interest and considered as a safe food for wheat related disorders. Besides the gluten, α-amylase/trypsin-inhibitors (ATIs) have been identified as probable candidates for these disorders. Several studies focused on wheat-ATIs although there is still a lack of data referring to the relative abundance of sorghum-ATIs. The objective of this work was therefore to contribute to the characterization of sorghum ATI profiles by targeted proteomics tools. Fifteen sorghum cultivars from different regions were investigated with raw proteins ranging from 7.9 to 17.0 g/100 g. Ammonium bicarbonate buffer in combination with urea was applied for protein extraction, with concentration from 0.588 ± 0.047 to 4.140 ± 0.066 mg/mL. Corresponding electrophoresis data showed different protein profiles. UniProtKB data base research reveals two sorghum ATIs, P81367 and P81368; both reviewed and a targeted LC–MS/MS method was developed to analyze these. Quantifier peptides ELAAVPSR (P81367) and TYMVR (P81368) were identified and retained as biomarkers for relative quantification. Different reducing and alkylating agents were assessed and combination of tris (2 carboxyethyl) phosphine/iodoacetamide gave the best response. Linearity was demonstrated for the quantifier peptides with standard recovery between 92.2 and 107.6%. Nine sorghum cultivars presented up to 60 times lower ATI contents as compared to wheat samples. This data suggests that sorghum can effectively be considered as a good alternative to wheat.
KW  - sorghum
KW  - α-amylase/trypsin inhibitors
KW  - reducing agents
KW  - cysteine alkylation
KW  - SDS PAGE
KW  - targeted proteomics
KW  - LC–MS/MS
Y1  - 2020
U6  - https://doi.org/10.3390/molecules25245982
SN  - 1420-3049
VL  - 25
IS  - 24
PB  - MDPI
CY  - Basel
ER  - 
TY  - GEN
A1  - Sagu Tchewonpi, Sorel
A1  - Landgräber, Eva
A1  - Rackiewicz, Michal
A1  - Huschek, Gerd
A1  - Rawel, Harshadrai Manilal
T1  - Relative Abundance of Alpha-Amylase/Trypsin Inhibitors in Selected Sorghum Cultivars
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Sorghum is of growing interest and considered as a safe food for wheat related disorders. Besides the gluten, α-amylase/trypsin-inhibitors (ATIs) have been identified as probable candidates for these disorders. Several studies focused on wheat-ATIs although there is still a lack of data referring to the relative abundance of sorghum-ATIs. The objective of this work was therefore to contribute to the characterization of sorghum ATI profiles by targeted proteomics tools. Fifteen sorghum cultivars from different regions were investigated with raw proteins ranging from 7.9 to 17.0 g/100 g. Ammonium bicarbonate buffer in combination with urea was applied for protein extraction, with concentration from 0.588 ± 0.047 to 4.140 ± 0.066 mg/mL. Corresponding electrophoresis data showed different protein profiles. UniProtKB data base research reveals two sorghum ATIs, P81367 and P81368; both reviewed and a targeted LC–MS/MS method was developed to analyze these. Quantifier peptides ELAAVPSR (P81367) and TYMVR (P81368) were identified and retained as biomarkers for relative quantification. Different reducing and alkylating agents were assessed and combination of tris (2 carboxyethyl) phosphine/iodoacetamide gave the best response. Linearity was demonstrated for the quantifier peptides with standard recovery between 92.2 and 107.6%. Nine sorghum cultivars presented up to 60 times lower ATI contents as compared to wheat samples. This data suggests that sorghum can effectively be considered as a good alternative to wheat.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1068 
KW  - sorghum
KW  - α-amylase/trypsin inhibitors
KW  - reducing agents
KW  - cysteine alkylation
KW  - SDS PAGE
KW  - targeted proteomics
KW  - LC–MS/MS
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-488096
SN  - 1866-8372
IS  - 1068
ER  -