TY  - JOUR
A1  - Brosnan, Sarah M.
A1  - Schlaad, Helmut
A1  - Antonietti, Markus
T1  - Aqueous Self-Assembly of Purely Hydrophilic Block Copolymers into Giant Vesicles
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - Self-assembly of macromolecules is fundamental to life itself, and historically, these systems have been primitively mimicked by the development of amphiphilic systems, driven by the hydrophobic effect. Herein, we demonstrate that self-assembly of purely hydrophilic systems can be readily achieved with similar ease and success. We have synthesized double hydrophilic block copolymers from polysaccharides and poly(ethylene oxide) or poly(sarcosine) to yield high molar mass diblock copolymers through oxime chemistry. These hydrophilic materials can easily assemble into nanosized (<500nm) and microsized (>5m) polymeric vesicles depending on concentration and diblock composition. Because of the solely hydrophilic nature of these materials, we expect them to be extraordinarily water permeable systems that would be well suited for use as cellular mimics.
KW  - block copolymers
KW  - polymersomes
KW  - polysaccharides
KW  - self-assembly
KW  - vesicles
Y1  - 2015
U6  - https://doi.org/10.1002/anie.201502100
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 33
SP  - 9715
EP  - 9718
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Bourgat, Yannick
A1  - Tiersch, Brigitte
A1  - Koetz, Joachim
A1  - Menzel, Henning
T1  - Enzyme degradable polymersomes from chitosan-g-[poly-l-lysine-block-epsilon-caprolactone] copolymer
JF  - Macromolecular bioscience
N2  - The scope of this study includes the synthesis of chitosan-g-[peptide-poly-epsilon-caprolactone] and its self-assembly into polymeric vesicles employing the solvent shift method. In this way, well-defined core-shell structures suitable for encapsulation of drugs are generated. The hydrophobic polycaprolactone side-chain and the hydrophilic chitosan backbone are linked via an enzyme-cleavable peptide. The synthetic route involves the functionalization of chitosan with maleimide groups and the preparation of polycaprolactone with alkyne end-groups. A peptide functionalized with a thiol group on one side and an azide group on the other side is prepared. Thiol-ene click-chemistry and azide-alkyne Huisgen cycloaddition are then used to link the chitosan and poly-epsilon-caprolactone chains, respectively, with this peptide. For a preliminary study, poly-l-lysin is a readily available and cleavable peptide that is introduced to investigate the feasibility of the system. The size and shape of the polymersomes are studied by dynamic light scattering and cryo-scanning electron microscopy. Furthermore, degradability is studied by incubating the polymersomes with two enzymes, trypsin and chitosanase. A dispersion of polymersomes is used to coat titanium plates and to further test the stability against enzymatic degradation.
KW  - chitosan
KW  - click chemistry
KW  - drug delivery system
KW  - enzyme
KW  - polymersomes
KW  - poly‐ ε ‐ caprolactone
Y1  - 2020
U6  - https://doi.org/10.1002/mabi.202000259
SN  - 1616-5187
SN  - 1616-5195
VL  - 21
IS  - 1
SP  - 1
EP  - 9
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - GEN
A1  - Bourgat, Yannick
A1  - Tiersch, Brigitte
A1  - Koetz, Joachim
A1  - Menzel, Henning
T1  - Enzyme degradable polymersomes from chitosan-g-[poly-l-lysine-block-epsilon-caprolactone] copolymer
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - The scope of this study includes the synthesis of chitosan-g-[peptide-poly-epsilon-caprolactone] and its self-assembly into polymeric vesicles employing the solvent shift method. In this way, well-defined core-shell structures suitable for encapsulation of drugs are generated. The hydrophobic polycaprolactone side-chain and the hydrophilic chitosan backbone are linked via an enzyme-cleavable peptide. The synthetic route involves the functionalization of chitosan with maleimide groups and the preparation of polycaprolactone with alkyne end-groups. A peptide functionalized with a thiol group on one side and an azide group on the other side is prepared. Thiol-ene click-chemistry and azide-alkyne Huisgen cycloaddition are then used to link the chitosan and poly-epsilon-caprolactone chains, respectively, with this peptide. For a preliminary study, poly-l-lysin is a readily available and cleavable peptide that is introduced to investigate the feasibility of the system. The size and shape of the polymersomes are studied by dynamic light scattering and cryo-scanning electron microscopy. Furthermore, degradability is studied by incubating the polymersomes with two enzymes, trypsin and chitosanase. A dispersion of polymersomes is used to coat titanium plates and to further test the stability against enzymatic degradation.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1382 
KW  - chitosan
KW  - click chemistry
KW  - drug delivery system
KW  - enzyme
KW  - polymersomes
KW  - poly‐ ε ‐ caprolactone
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-566584
SN  - 1866-8372
IS  - 1
ER  -