TY  - JOUR
A1  - Luckert, Claudia
A1  - Hessel, Stefanie
A1  - Lampen, Alfonso
A1  - Braeuning, Albert
T1  - Utility of an appropriate reporter assay: Heliotrine interferes with GAL4/upstream activation sequence-driven reporter gene systems
JF  - Analytical biochemistry : methods in the biological sciences
N2  - Reporter gene assays are widely used for the assessment of transcription factor activation following xenobiotic exposure of cells. A critical issue with such assays is the possibility of interference of test compounds with the test system, for example, by direct inhibition of the reporter enzyme. Here we show that the pyrrolizidine alkaloid heliotrine interferes with reporter signals derived from GAL4-based nuclear receptor transactivation assays by a mechanism independent of luciferase enzyme inhibition. These data highlight the necessity to conduct proper control experiments in order to avoid perturbation of reporter assays by test chemicals. (C) 2015 Elsevier Inc. All rights reserved.
KW  - Firefly luciferase inhibition
KW  - Nuclear receptor
KW  - Transactivation assay
Y1  - 2015
U6  - https://doi.org/10.1016/j.ab.2015.07.009
SN  - 0003-2697
SN  - 1096-0309
VL  - 487
SP  - 45
EP  - 48
PB  - Elsevier
CY  - San Diego
ER  -