TY  - JOUR
A1  - Abdirashid, Hashim
A1  - Lenhard, Michael
T1  - Say it with double flowers
JF  - Journal of experimental botany
N2  - Every year, lovers world-wide rely on mutants to show their feelings on Valentine's Day. This is because many of the most popular ornamental flowering plants have been selected to form extra petals at the expense of reproductive organs to enhance their attractiveness and aesthetic value to humans. This so-called 'double flower' (DF) phenotype, first described more than 2000 years ago (Meyerowitz et al., 1989) is present, for example, in many modern roses, carnations, peonies, and camellias. Gattolin et al. (2020) now identify a unifying explanation for the molecular basis of many of these DF cultivars.
KW  - ABCE model
KW  - APETALA2
KW  - double flowers
KW  - flower development
KW  - homoeotic
KW  - mutants
KW  - microRNA172
Y1  - 2020
U6  - https://doi.org/10.1093/jxb/eraa109
SN  - 0022-0957
SN  - 1460-2431
VL  - 71
IS  - 9
SP  - 2469
EP  - 2471
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Abercrombie, Laura Good
A1  - Anderson, Cynthia M.
A1  - Baldwin, Bruce G.
A1  - Bang, In-Chul
A1  - Beldade, Ricardo
A1  - Bernardi, Giacomo
A1  - Boubou, Angham
A1  - Branca, Antoine
A1  - Bretagnolle, Francois
A1  - Bruford, Michael W.
A1  - Buonamici, Anna
A1  - Burnett, Robert K.
A1  - Canal, D.
A1  - Cardenas, H.
A1  - Caullet, Coraline
A1  - Chen, S. Y.
A1  - Chun, Y. J.
A1  - Cossu, C.
A1  - Crane, Charles F.
A1  - Cros-Arteil, Sandrine
A1  - Cudney-Bueno, Richard
A1  - Danti, Roberto
A1  - Davila, José Antonio
A1  - Della Rocca, Gianni
A1  - Dobata, Shigeto
A1  - Dunkle, Larry D.
A1  - Dupas, Stephane and others
T1  - Permanent genetic resources added to molecular ecology resources database 1 January 2009-30 April 2009
N2  - This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta; Ambrosia artemisiifolia; Berula erecta; Casuarius casuarius; Cercospora zeae-maydis; Chorthippus parallelus; Conyza canadensis; Cotesia sesamiae; Epinephelus acanthistius; Ficedula hypoleuca; Grindelia hirsutula; Guadua angustifolia; Leucadendron rubrum; Maritrema novaezealandensis; Meretrix meretrix; Nilaparvata lugens; Oxyeleotris marmoratus; Phoxinus neogaeus; Pristomyrmex punctatus; Pseudobagrus brevicorpus; Seiridium cardinale; Stenopsyche marmorata; Tetranychus evansi and Xerus inauris. These loci were cross-tested on the following species: Agalinis decemloba; Agalinis tenella; Agalinis obtusifolia; Agalinis setacea; Agalinis skinneriana; Cercospora zeina; Cercospora kikuchii; Cercospora sorghi; Mycosphaerella graminicola; Setosphaeria turcica; Magnaporthe oryzae; Cotesia flavipes; Cotesia marginiventris; Grindelia Xpaludosa; Grindelia chiloensis; Grindelia fastigiata; Grindelia lanceolata; Grindelia squarrosa; Leucadendron coniferum; Leucadendron salicifolium; Leucadendron tinctum; Leucadendron meridianum; Laodelphax striatellus; Sogatella furcifera; Phoxinus eos; Phoxinus rigidus; Phoxinus brevispinosus; Phoxinus bicolor; Tetranychus urticae; Tetranychus turkestani; Tetranychus ludeni; Tetranychus neocaledonicus; Tetranychus amicus; Amphitetranychus viennensis; Eotetranychus rubiphilus; Eotetranychus tiliarium; Oligonychus perseae; Panonychus citri; Bryobia rubrioculus; Schizonobia bundi; Petrobia harti; Xerus princeps; Spermophilus tridecemlineatus and Sciurus carolinensis.
Y1  - 2009
UR  - http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291755-0998
U6  - https://doi.org/10.1111/j.1755-0998.2009.02746.x
SN  - 1755-098X
ER  - 
TY  - JOUR
A1  - Aberle-Malzahn, Nicole
A1  - Bauer, Barbara
A1  - Lewandowska, A.
A1  - Gaedke, Ursula
A1  - Sommer, U.
T1  - Warming induces shifts in microzooplankton phenology and reduces time-lags between phytoplankton and protozoan production
JF  - Marine biology : international journal on life in oceans and coastal waters
N2  - Indoor mesocosm experiments were conducted to test for potential climate change effects on the spring succession of Baltic Sea plankton. Two different temperature (Delta 0 A degrees C and Delta 6 A degrees C) and three light scenarios (62, 57 and 49 % of the natural surface light intensity on sunny days), mimicking increasing cloudiness as predicted for warmer winters in the Baltic Sea region, were simulated. By combining experimental and modeling approaches, we were able to test for a potential dietary mismatch between phytoplankton and zooplankton. Two general predator-prey models, one representing the community as a tri-trophic food chain and one as a 5-guild food web were applied to test for the consequences of different temperature sensitivities of heterotrophic components of the plankton. During the experiments, we observed reduced time-lags between the peaks of phytoplankton and protozoan biomass in response to warming. Microzooplankton peak biomass was reached by 2.5 day A degrees C-1 earlier and occurred almost synchronously with biomass peaks of phytoplankton in the warm mesocosms (Delta 6 A degrees C). The peak magnitudes of microzooplankton biomass remained unaffected by temperature, and growth rates of microzooplankton were higher at Delta 6 A degrees C (mu(a dagger 0 A degrees C) = 0.12 day(-1) and mu(a dagger 6 A degrees C) = 0.25 day(-1)). Furthermore, warming induced a shift in microzooplankton phenology leading to a faster species turnover and a shorter window of microzooplankton occurrence. Moderate differences in the light levels had no significant effect on the time-lags between autotrophic and heterotrophic biomass and on the timing, biomass maxima and growth rate of microzooplankton biomass. Both models predicted reduced time-lags between the biomass peaks of phytoplankton and its predators (both microzooplankton and copepods) with warming. The reduction of time-lags increased with increasing Q(10) values of copepods and protozoans in the tritrophic food chain. Indirect trophic effects modified this pattern in the 5-guild food web. Our study shows that instead of a mismatch, warming might lead to a stronger match between protist grazers and their prey altering in turn the transfer of matter and energy toward higher trophic levels.
Y1  - 2012
U6  - https://doi.org/10.1007/s00227-012-1947-0
SN  - 0025-3162
VL  - 159
IS  - 11
SP  - 2441
EP  - 2453
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Abraham, Andreas
A1  - Gruss, Michael
T1  - Stress inoculation facilitates active avoidance learning of the semi-precocial rodent Octodon degus
N2  - A growing body of evidence highlights the impact of the early social environment for the adequate development of brain and behavior in animals and humans. Disturbances of this environment were found to be both maladaptive and adaptive to emotional and cognitive function. Using the semi-precocial, biparental rodent Octodon degus, we aimed to examine (i) the impact of age (juvenile/adult), sex (male/female), and (ii) "motivation" to solve the task (by applying increasing foot-shock-intensities) on two-way active avoidance (TWA) learning in socially reared degus, and (iii) whether early life stress inoculation by 1 h daily parental separation during the first three weeks of life has maladaptive or adaptive consequences on cognitive function as measured by TWA learning. Our results showed that (i) juvenile degus, unlike altricial rats of the same age, can successfully learn the TWA task comparable to adults, and (ii) that learning performance improves with increasing "task motivation", irrespective of age and sex. Furthermore, we revealed that (iii) stress inoculation improves avoidance learning, particularly in juvenile males, quantitatively and qualitatively depending on "task motivation". In conclusion, the present study describes for the first time associative learning in O. degus and its modulation by early life stress experience as an animal model to study the underlying mechanisms of learning and memory in the stressed and unstressed brain. Although, stress is commonly viewed as being maladaptive, our data indicate that early life stress inoculation triggers developmental cascades of adaptive functioning, which may improve cognitive and emotional processing of stressors later in life.
Y1  - 2010
UR  - http://www.sciencedirect.com/science/journal/01664328
U6  - https://doi.org/10.1016/j.bbr.2010.05.018
SN  - 0166-4328
ER  - 
TY  - JOUR
A1  - Abrahamczyk, Stefan
A1  - Lozada Gobilard, Sissi Donna
A1  - Ackermann, Markus
A1  - Fischer, Eberhard
A1  - Krieger, Vera
A1  - Redling, Almut
A1  - Weigend, Maximilian
T1  - A question of data quality-Testing pollination syndromes in Balsaminaceae
JF  - PLoS one
N2  - Pollination syndromes and their predictive power regarding actual plant-animal interactions have been controversially discussed in the past. We investigate pollination syndromes in Balsaminaceae, utilizing quantitative respectively categorical data sets of flower morphometry, signal and reward traits for 86 species to test for the effect of different types of data on the test patterns retrieved. Cluster Analyses of the floral traits are used in combination with independent pollinator observations. Based on quantitative data we retrieve seven clusters, six of them corresponding to plausible pollination syndromes and one additional, well-supported cluster comprising highly divergent floral architectures. This latter cluster represents a non-syndrome of flowers not segregated by the specific data set here used. Conversely, using categorical data we obtained only a rudimentary resolution of pollination syndromes, in line with several earlier studies. The results underscore that the use of functional, exactly quanitified trait data has the power to retrieve pollination syndromes circumscribed by the specific data used. Data quality can, however, not be replaced by sheer data volume. With this caveat, it is possible to identify pollination syndromes from large datasets and to reliably extrapolate them for taxa for which direct observations are unavailable.
Y1  - 2017
U6  - https://doi.org/10.1371/journal.pone.0186125
SN  - 1932-6203
VL  - 12
PB  - PLoS
CY  - San Fransisco
ER  - 
TY  - JOUR
A1  - Acker, Helmut
A1  - Huckstorf, Christine
A1  - Sauer, Heinrich
A1  - Streller, Tino
A1  - Wartenberg, Maria
T1  - Deciphering the oxygen sensing pathway by microscopy
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Ackermann, Margit
T1  - Funktionskontrolle einer ACO-Amphibienschutzanlage
Y1  - 2003
SN  - 3-933066-16-6
ER  - 
TY  - JOUR
A1  - Adamla, Frauke
A1  - Ignatova, Zoya
T1  - Somatic expression of unc-54 and vha-6 mRNAs declines but not pan-neuronal rgef-1 and unc-119 expression in aging Caenorhabditis elegans
JF  - Scientific reports
N2  - Aging is a highly controlled biological process characterized by a progressive deterioration of various cellular activities. One of several hallmarks of aging describes a link to transcriptional alteration, suggesting that it may impact the steady-state mRNA levels. We analyzed the mRNA steady-state levels of polyCAG-encoding transgenes and endogenous genes under the control of well-characterized promoters for intestinal (vha-6), muscular (unc-54, unc-15) and pan-neuronal (rgef-1, unc-119) expression in the nematode Caenorhabditis elegans. We find that there is not a uniform change in transcriptional profile in aging, but rather a tissue-specific difference in the mRNA levels of these genes. While levels of mRNA in the intestine (vha-6) and muscular (unc-54, unc-15) cells decline with age, pan-neuronal tissue shows more stable mRNA expression (rgef-1, unc-119) which even slightly increases with the age of the animals. Our data on the variations in the mRNA abundance from exemplary cases of endogenous and transgenic gene expression contribute to the emerging evidence for tissue-specific variations in the aging process.
Y1  - 2015
U6  - https://doi.org/10.1038/srep10692
SN  - 2045-2322
VL  - 5
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Adel, Mustafa
A1  - Elbehery, Ali H. A.
A1  - Aziz, Sherry K.
A1  - Aziz, Ramy K.
A1  - Grossart, Hans-Peter
A1  - Siam, Rania
T1  - Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments
JF  - Scientific reports
N2  - The central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collected from ATIID, DD, CD brine pools and an adjacent brine-influenced site. Sixteen different lithologic sediment sections were subjected to shotgun DNA pyrosequencing to generate 1.47 billion base pairs (1.47 x 10(9) bp). We generated sediment-specific reads and attempted to annotate all reads. We report the phylogenetic and biochemical uniqueness of the deepest ATIID sulfur-rich brine pool sediments. In contrary to all other sediment sections, bacteria dominate the deepest ATIID sulfur-rich brine pool sediments. This decrease in virus-to-bacteria ratio in selected sections and depth coincided with an overrepresentation of mobile genetic elements. Skewing in the composition of viruses-to-mobile genetic elements may uniquely contribute to the distinct microbial consortium in sediments in proximity to hydrothermally active vents of the Red Sea and possibly in their surroundings, through differential horizontal gene transfer.
Y1  - 2016
U6  - https://doi.org/10.1038/srep32704
SN  - 2045-2322
VL  - 6
SP  - 8882
EP  - 8888
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Adem, Fozia A.
A1  - Kuete, Victor
A1  - Mbaveng, Armelle T.
A1  - Heydenreich, Matthias
A1  - Koch, Andreas
A1  - Ndakala, Albert
A1  - Irungu, Beatrice
A1  - Yenesew, Abiy
A1  - Efferth, Thomas
T1  - Cytotoxic flavonoids from two Lonchocarpus species
JF  - Natural Product Research
N2  - A new isoflavone, 4′-prenyloxyvigvexin A (1) and a new pterocarpan, (6aR,11aR)-3,8-dimethoxybitucarpin B (2) were isolated from the leaves of Lonchocarpus bussei and the stem bark of Lonchocarpus eriocalyx, respectively. The extract of L. bussei also gave four known isoflavones, maximaisoflavone H, 7,2′-dimethoxy-3′,4′-methylenedioxyisoflavone, 6,7,3′-trimethoxy-4′,5′-methylenedioxyisoflavone, durmillone; a chalcone, 4-hydroxylonchocarpin; a geranylated phenylpropanol, colenemol; and two known pterocarpans, (6aR,11aR)-maackiain and (6aR,11aR)-edunol. (6aR,11aR)-Edunol was also isolated from the stem bark of L. eriocalyx. The structures of the isolated compounds were elucidated by spectroscopy. The cytotoxicity of the compounds was tested by resazurin assay using drug-sensitive and multidrug-resistant cancer cell lines. Significant antiproliferative effects with IC50 values below 10 μM were observed for the isoflavones 6,7,3′-trimethoxy-4′,5′-methylenedioxyisoflavone and durmillone against leukemia CCRF-CEM cells; for the chalcone, 4-hydroxylonchocarpin and durmillone against its resistant counterpart CEM/ADR5000 cells; as well as for durmillone against the resistant breast adenocarcinoma MDA-MB231/BCRP cells and resistant gliobastoma U87MG.ΔEGFR cells.
KW  - Lonchocarpus bussei
KW  - Lonchocarpus eriocalyx
KW  - Leguminosae
KW  - isoflavone
KW  - pterocarpan
KW  - cytotoxicity
Y1  - 2019
U6  - https://doi.org/10.1080/14786419.2018.1462179
SN  - 1478-6419
SN  - 1478-6427
VL  - 33
IS  - 18
SP  - 2609
EP  - 2617
PB  - Routledge, Taylor & Francis Group
CY  - Abingdon
ER  - 
TY  - JOUR
A1  - Adem, Fozia A.
A1  - Kuete, Victor
A1  - Mbaveng, Armelle T.
A1  - Heydenreich, Matthias
A1  - Ndakala, Albert
A1  - Irungu, Beatrice
A1  - Efferth, Thomas
A1  - Yenesew, Abiy
T1  - Cytotoxic benzylbenzofuran derivatives from Dorstenia kameruniana
JF  - Fitoterapia
N2  - Chromatographic separation of the extract of the roots of Dorstenia kameruniana (family Moraceae) led to the isolation of three new benzylbenzofuran derivatives, 2-(p-hydroxybenzyl)benzofuran-6-ol (1), 2-(p-hydroxybenzyl)-7-methoxybenzofuran-6-ol (2) and 2-(p-hydroxy)-3-(3-methylbut-2-en-1-yl)benzyl)benzofuran-6-ol (3) (named dorsmerunin A, B and C, respectively), along with the known furanocoumarin, bergapten (4). The twigs of Dorstenia kameruniana also produced compounds 1-4 as well as the known chalcone licoagrochalcone A (5). The structures were elucidated by NMR spectroscopy and mass spectrometry. The isolated compounds displayed cytotoxicity against the sensitive CCRF-CEM and multidrug-resistant CEM/ADR5000 leukemia cells, where compounds 4 and 5 had the highest activities (IC50 values of 7.17 mu M and 5.16 mu M, respectively) against CCRF-CEM leukemia cells. Compound 5 also showed cytotoxicity against 7 sensitive or drug-resistant solid tumor cell lines (breast carcinoma, colon carcinoma, glioblastoma), with IC50 below 50 mu M, whilst 4 showed selective activity.
KW  - Dorstenia kameruniana
KW  - Moraceae
KW  - Benzylbenzofuran
KW  - Furanocoumarin
KW  - Chalcone
KW  - Cytotoxicity
Y1  - 2018
U6  - https://doi.org/10.1016/j.fitote.2018.04.019
SN  - 0367-326X
SN  - 1873-6971
VL  - 128
SP  - 26
EP  - 30
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Agarwal, Saloni
A1  - Warmt, Christian
A1  - Henkel, Jörg
A1  - Schrick, Livia
A1  - Nitsche, Andreas
A1  - Bier, Frank Fabian
T1  - Lateral flow-based nucleic acid detection of SARS-CoV-2 using enzymatic incorporation of biotin-labeled dUTP for POCT use
JF  - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry, Analusis and Quimica analitica
N2  - The degree of detrimental effects inflicted on mankind by the COVID-19 pandemic increased the need to develop ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment-free, and Deliverable) POCT (point of care testing) to overcome the current and any future pandemics. Much effort in research and development is currently advancing the progress to overcome the diagnostic pressure built up by emerging new pathogens. LAMP (loop-mediated isothermal amplification) is a well-researched isothermal technique for specific nucleic acid amplification which can be combined with a highly sensitive immunochromatographic readout via lateral flow assays (LFA). Here we discuss LAMP-LFA robustness, sensitivity, and specificity for SARS-CoV-2 N-gene detection in cDNA and clinical swab-extracted RNA samples. The LFA readout is designed to produce highly specific results by incorporation of biotin and FITC labels to 11-dUTP and LF (loop forming forward) primer, respectively. The LAMP-LFA assay was established using cDNA for N-gene with an accuracy of 95.65%. To validate the study, 82 SARS-CoV-2-positive RNA samples were tested. Reverse transcriptase (RT)-LAMP-LFA was positive for the RNA samples with an accuracy of 81.66%; SARS-CoV-2 viral RNA was detected by RT-LAMP-LFA for as low as CT-33. Our method reduced the detection time to 15 min and indicates therefore that RT-LAMP in combination with LFA represents a promising nucleic acid biosensing POCT platform that combines with smartphone based semi-quantitative data analysis.
KW  - Point of care testing (POCT)
KW  - Lateral flow assay (LFA)
KW  - COVID-19
KW  - Reverse transcription loop-mediated isothermal amplification (RT-LAMP);
KW  - SARS-CoV-2 N-gene
Y1  - 2022
U6  - https://doi.org/10.1007/s00216-022-03880-4
SN  - 1618-2642
SN  - 1618-2650
VL  - 414
IS  - 10
SP  - 3177
EP  - 3186
PB  - Springer
CY  - Heidelberg
ER  - 
TY  - JOUR
A1  - Agne, Stefanie
A1  - Preick, Michaela
A1  - Straube, Nicolas
A1  - Hofreiter, Michael
T1  - Simultaneous Barcode Sequencing of Diverse Museum Collection Specimens Using a Mixed RNA Bait Set
JF  - Frontiers in Ecology and Evolution
N2  - A growing number of publications presenting results from sequencing natural history collection specimens reflect the importance of DNA sequence information from such samples. Ancient DNA extraction and library preparation methods in combination with target gene capture are a way of unlocking archival DNA, including from formalin-fixed wet-collection material. Here we report on an experiment, in which we used an RNA bait set containing baits from a wide taxonomic range of species for DNA hybridisation capture of nuclear and mitochondrial targets for analysing natural history collection specimens. The bait set used consists of 2,492 mitochondrial and 530 nuclear RNA baits and comprises specific barcode loci of diverse animal groups including both invertebrates and vertebrates. The baits allowed to capture DNA sequence information of target barcode loci from 84% of the 37 samples tested, with nuclear markers being captured more frequently and consensus sequences of these being more complete compared to mitochondrial markers. Samples from dry material had a higher rate of success than wet-collection specimens, although target sequence information could be captured from 50% of formalin-fixed samples. Our study illustrates how efforts to obtain barcode sequence information from natural history collection specimens may be combined and are a way of implementing barcoding inventories of scientific collection material.
KW  - target capture
KW  - type specimens
KW  - molecular species identification
KW  - museum specimens
KW  - cross-species capture
Y1  - 2022
U6  - https://doi.org/10.3389/fevo.2022.909846
SN  - 2296-701X
VL  - 10
PB  - Frontiers Media S.A.
CY  - Lausanne, Schweiz
ER  - 
TY  - JOUR
A1  - Ahmed, Muhammad N.
A1  - Reyna-Gonzalez, Emmanuel
A1  - Schmid, Bianca
A1  - Wiebach, Vincent
A1  - Suessmuth, Roderich D.
A1  - Dittmann, Elke
A1  - Fewer, David P.
T1  - Phylogenomic Analysis of the Microviridin Biosynthetic Pathway Coupled with Targeted Chemo-Enzymatic Synthesis Yields Potent Protease Inhibitors
JF  - ACS chemical biology
N2  - Natural products and their semisynthetic derivatives are an important source of drugs for the pharmaceutical industry. Bacteria are prolific producers of natural products and encode a vast diversity of natural product biosynthetic gene clusters. However, much of this diversity is inaccessible to natural product discovery. Here, we use a combination of phylogenomic analysis of the microviridin biosynthetic pathway and chemo-enzymatic synthesis of bioinformatically predicted microviridins to yield new protease inhibitors. Phylogenomic analysis demonstrated that microviridin biosynthetic gene clusters occur across the bacterial domain and encode three distinct subtypes of precursor peptides. Our analysis shed light on the evolution of microviridin biosynthesis and enabled prioritization of their chemo-enzymatic production. Targeted one-pot synthesis of four microviridins encoded by the cyanobacterium Cyanothece sp. PCC 7822 identified a set of novel and potent serine protease inhibitors, the most active of which had an IC50 value of 21.5 nM. This study advances the genome mining techniques available for natural product discovery and obviates the need to culture bacteria.
Y1  - 2017
U6  - https://doi.org/10.1021/acschembio.7b00124
SN  - 1554-8929
SN  - 1554-8937
VL  - 12
SP  - 1538
EP  - 1546
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Aichner, Bernhard
A1  - Dubbert, David
A1  - Kiel, Christine
A1  - Kohnert, Katrin
A1  - Ogashawara, Igor
A1  - Jechow, Andreas
A1  - Harpenslager, Sarah-Faye
A1  - Hölker, Franz
A1  - Nejstgaard, Jens Christian
A1  - Grossart, Hans-Peter
A1  - Singer, Gabriel
A1  - Wollrab, Sabine
A1  - Berger, Stella Angela
T1  - Spatial and seasonal patterns of water isotopes in northeastern German lakes
JF  - Earth system science data : ESSD
N2  - Water stable isotopes (delta O-18 and delta H-2) were analyzed in samples collected in lakes, associated with riverine systems in northeastern Germany, throughout 2020. The dataset (Aichner et al., 2021; https://doi.org/10.1594/PANGAEA.935633) is derived from water samples collected at (a) lake shores (sampled in March and July 2020), (b) buoys which were temporarily installed in deep parts of the lake (sampled monthly from March to October 2020), (c) multiple spatially distributed spots in four selected lakes (in September 2020), and (d) the outflow of Muggelsee (sampled biweekly from March 2020 to January 2021). At shores, water was sampled with a pipette from 40-60 cm below the water surface and directly transferred into a measurement vial, while at buoys a Limnos water sampler was used to obtain samples from 1 m below the surface. Isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer, with a measurement uncertainty of < 0.15 parts per thousand (delta O-18) and < 0.0 parts per thousand (delta H-2). The data give information about the vegetation period and the full seasonal isotope amplitude in the sampled lakes and about spatial isotope variability in different branches of the associated riverine systems.
Y1  - 2022
U6  - https://doi.org/10.5194/essd-14-1857-2022
SN  - 1866-3508
SN  - 1866-3516
VL  - 14
IS  - 4
SP  - 1857
EP  - 1867
PB  - Copernicus
CY  - Göttingen
ER  - 
TY  - JOUR
A1  - Aksu, Yilmaz
A1  - Frasca, Stefano
A1  - Wollenberger, Ursula
A1  - Driess, Matthias
A1  - Thomas, Arne
T1  - A molecular precursor approach to tunable porous tin-rich indium tin oxide with durable high electrical conductivity for bioelectronic devices
JF  - Chemistry of materials : a publication of the American Chemical Society
N2  - The preparation of porous, i.e., high surface area electrodes from transparent conducting oxides, is a valuable goal in materials chemistry as such electrodes can enable further development of optoelectronic, electrocatalytic, or bioelectronic devices. In this work the first tin-rich mesoporous indium tin oxide is prepared using the molecular heterobimetallic single-source precursor, indium tin tris-tert-butoxide, together with an appropriate structure-directing template, yielding materials with high surface areas and tailorable pore size. The resulting mesoporous tin-rich ITO films show a high and durable electrical conductivity and transparency, making them interesting materials for hosting electroactive biomolecules such as proteins. In fact, its unique performance in bioelectronic applications has been demonstrated by immobilization of high amounts of cytochrome c into the mesoporous film which undergo redox processes directly with the conductive electrode material.
KW  - indium tin oxide ITO
KW  - electrode
KW  - bioelectrochemistry
KW  - device
KW  - cytochrome c
Y1  - 2011
U6  - https://doi.org/10.1021/cm103087p
SN  - 0897-4756
VL  - 23
IS  - 7
SP  - 1798
EP  - 1804
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - JOUR
A1  - Albers, Philip
A1  - Üstün, Suayib
A1  - Witzel, Katja
A1  - Kraner, Max Erdmund
A1  - Börnke, Frederik
T1  - A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1
JF  - Molecular Plant-Microbe Interactions
N2  - The plasma membrane (PM) is at the interface of plant-pathogen interactions and, thus, many bacterial type-III effector (T3E) proteins target membrane-associated processes to interfere with immunity. The Pseudomonas syringae T3E HopZ1a is a host cell PM-localized effector protein that has several immunity-associated host targets but also activates effector-triggered immunity in resistant backgrounds. Although HopZ1a has been shown to interfere with early defense signaling at the PM, no dedicated PM-associated HopZ1a target protein has been identified until now. Here, we show that HopZ1a interacts with the PM-associated remorin protein NbREM4 from Nicotiana benthamiana in several independent assays. NbREM4 relocalizes to membrane nanodomains after treatment with the bacterial elicitor flg22 and transient overexpression of NbREM4 in N. benthamiana induces the expression of a subset of defense-related genes. We can further show that NbREM4 interacts with the immune-related receptor-like cytoplasmic kinase avrPphB-susceptible 1 (PBS1) and is phosphorylated by PBS1 on several residues in vitro. Thus, we conclude that NbREM4 is associated with early defense signaling at the PM. The possible relevance of the HopZ1a-NbREM4 interaction for HopZ1a virulence and avirulence functions is discussed.
KW  - bacterial pathogenesis
KW  - defense signaling pathways
KW  - effectors
KW  - elicitors
KW  - HopZ1a
KW  - MAMPs
KW  - PAMPs
KW  - PBS1
KW  - Pseudomonas syringae
KW  - remorin
KW  - type-3 secretion
Y1  - 2019
U6  - https://doi.org/10.1094/MPMI-04-19-0105-R
SN  - 0894-0282
SN  - 1943-7706
VL  - 32
IS  - 9
SP  - 1229
EP  - 1242
PB  - Amer phytopathological  SOC
CY  - ST Paul
ER  - 
TY  - JOUR
A1  - Albert, Aurelie
A1  - Auffret, Alistair G.
A1  - Cosyns, Eric
A1  - Cousins, Sara A. O.
A1  - Eichberg, Carsten
A1  - Eycott, Amy E.
A1  - Heinken, Thilo
A1  - Hoffmann, Maurice
A1  - Jaroszewicz, Bogdan
A1  - Malo, Juan E.
A1  - Marell, Anders
A1  - Mouissie, Maarten
A1  - Pakeman, Robin J.
A1  - Picard, Melanie
A1  - Plue, Jan
A1  - Poschlod, Peter
A1  - Provoost, Sam
A1  - Schulze, Kiowa Alraune
A1  - Baltzinger, Christophe
T1  - Seed dispersal by ungulates as an ecological filter: a trait-based meta-analysis
JF  - Oikos
N2  - Plant communities are often dispersal-limited and zoochory can be an efficient mechanism for plants to colonize new patches of potentially suitable habitat. We predicted that seed dispersal by ungulates acts as an ecological filter - which differentially affects individuals according to their characteristics and shapes species assemblages - and that the filter varies according to the dispersal mechanism (endozoochory, fur-epizoochory and hoof-epizoochory). We conducted two-step individual participant data meta-analyses of 52 studies on plant dispersal by ungulates in fragmented landscapes, comparing eight plant traits and two habitat indicators between dispersed and non-dispersed plants. We found that ungulates dispersed at least 44% of the available plant species. Moreover, some plant traits and habitat indicators increased the likelihood for plant of being dispersed. Persistent or nitrophilous plant species from open habitats or bearing dry or elongated diaspores were more likely to be dispersed by ungulates, whatever the dispersal mechanism. In addition, endozoochory was more likely for diaspores bearing elongated appendages whereas epizoochory was more likely for diaspores released relatively high in vegetation. Hoof-epizoochory was more likely for light diaspores without hooked appendages. Fur-epizoochory was more likely for diaspores with appendages, particularly elongated or hooked ones. We thus observed a gradient of filtering effect among the three dispersal mechanisms. Endozoochory had an effect of rather weak intensity (impacting six plant characteristics with variations between ungulate-dispersed and non-dispersed plant species mostly below 25%), whereas hoof-epizoochory had a stronger effect (eight characteristics included five ones with above 75% variation), and fur-epizoochory an even stronger one (nine characteristics included six ones with above 75% variation). Our results demonstrate that seed dispersal by ungulates is an ecological filter whose intensity varies according to the dispersal mechanism considered. Ungulates can thus play a key role in plant community dynamics and have implications for plant spatial distribution patterns at multiple scales.
Y1  - 2015
U6  - https://doi.org/10.1111/oik.02512
SN  - 0030-1299
SN  - 1600-0706
VL  - 124
IS  - 9
SP  - 1109
EP  - 1120
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Albert, Cécile H.
A1  - Grassein, Fabrice
A1  - Schurr, Frank Martin
A1  - Vieilledent, Ghislain
A1  - Violle, Cyrille
T1  - When and how should intraspecific variability be considered in trait-based plant ecology?
JF  - Perspectives in plant ecology, evolution and systematics
N2  - Trait-based studies have become extremely common in plant ecology. Trait-based approaches often rely on the tacit assumption that intraspecific trait variability (ITV) is negligible compared to interspecific variability, so that species can be characterized by mean trait values. Yet, numerous recent studies have challenged this assumption by showing that ITV significantly affects various ecological processes. Accounting for ITV may thus strengthen trait-based approaches, but measuring trait values on a large number of individuals per species and site is not feasible. Therefore, it is important and timely to synthesize existing knowledge on ITV in order to (1) decide critically when ITV should be considered, and (2) establish methods for incorporating this variability. Here we propose a practical set of rules to identify circumstances under which ITV should be accounted for. We formulate a spatial trait variance partitioning hypothesis to highlight the spatial scales at which ITV cannot be ignored in ecological studies. We then refine a set of four consecutive questions on the research question, the spatial scale, the sampling design, and the type of studied traits, to determine case-by-case if a given study should quantify ITV and test its effects. We review methods for quantifying ITV and develop a step-by-step guideline to design and interpret simulation studies that test for the importance of ITV. Even in the absence of quantitative knowledge on ITV, its effects can be assessed by varying trait values within species within realistic bounds around the known mean values. We finish with a discussion of future requirements to further incorporate ITV within trait-based approaches. This paper thus delineates a general framework to account for ITV and suggests a direction towards a more quantitative trait-based ecology.
KW  - Comparative ecology
KW  - Functional ecology
KW  - Genetic variability
KW  - Intraspecific functional variability
KW  - Phenotypic plasticity
KW  - Plant functional hairs
KW  - Within-species variability
Y1  - 2011
U6  - https://doi.org/10.1016/j.ppees.2011.04.003
SN  - 1433-8319
VL  - 13
IS  - 3
SP  - 217
EP  - 225
PB  - Elsevier
CY  - Jena
ER  - 
TY  - JOUR
A1  - Alberti, Federica
A1  - Gonzalez, Javier
A1  - Paijmans, Johanna L. A.
A1  - Basler, Nikolas
A1  - Preick, Michaela
A1  - Henneberger, Kirstin
A1  - Trinks, Alexandra
A1  - Rabeder, Gernot
A1  - Conard, Nicholas J.
A1  - Muenzel, Susanne C.
A1  - Joger, Ulrich
A1  - Fritsch, Guido
A1  - Hildebrandt, Thomas
A1  - Hofreiter, Michael
A1  - Barlow, Axel
T1  - Optimized DNA sampling of ancient bones using Computed Tomography scans
JF  - Molecular ecology resources
N2  - The prevalence of contaminant microbial DNA in ancient bone samples represents the principal limiting factor for palaeogenomic studies, as it may comprise more than 99% of DNA molecules obtained. Efforts to exclude or reduce this contaminant fraction have been numerous but also variable in their success. Here, we present a simple but highly effective method to increase the relative proportion of endogenous molecules obtained from ancient bones. Using computed tomography (CT) scanning, we identify the densest region of a bone as optimal for sampling. This approach accurately identifies the densest internal regions of petrous bones, which are known to be a source of high-purity ancient DNA. For ancient long bones, CT scans reveal a high-density outermost layer, which has been routinely removed and discarded prior to DNA extraction. For almost all long bones investigated, we find that targeted sampling of this outermost layer provides an increase in endogenous DNA content over that obtained from softer, trabecular bone. This targeted sampling can produce as much as 50-fold increase in the proportion of endogenous DNA, providing a directly proportional reduction in sequencing costs for shotgun sequencing experiments. The observed increases in endogenous DNA proportion are not associated with any reduction in absolute endogenous molecule recovery. Although sampling the outermost layer can result in higher levels of human contamination, some bones were found to have more contamination associated with the internal bone structures. Our method is highly consistent, reproducible and applicable across a wide range of bone types, ages and species. We predict that this discovery will greatly extend the potential to study ancient populations and species in the genomics era.
KW  - ancient DNA
KW  - computer tomography
KW  - palaeogenomics
KW  - paleogenetics
KW  - petrous bone
Y1  - 2018
U6  - https://doi.org/10.1111/1755-0998.12911
SN  - 1755-098X
SN  - 1755-0998
VL  - 18
IS  - 6
SP  - 1196
EP  - 1208
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Albrecht, Tanja
A1  - Greve, Burkhard
A1  - Pusch, Kerstin
A1  - Koßmann, Jens
A1  - Buchner, Peter
A1  - Wobus, Ulrich
A1  - Steup, Martin
T1  - Homo- and Heterodimers of Pho1-Type Phosphorylase Isoforms in Solanum tuberosum L. as Revealed by Sequence- Specific Antibodies
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Albrecht, Tanja
A1  - Haebel, Sophie
A1  - Koch, Anke
A1  - Krause, Ulrike
A1  - Eckermann, Nora
A1  - Steup, Martin
T1  - Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosin residues but results in a reduced bacterial glycogen accumulation
N2  - Saccharomyces cerevisiae possesses two glycogenin isoforms (designated as Glg1p and Glg2p) that both contain a conserved tyrosine residue, Tyr232. However, Glg2p possesses an additional tyrosine residue, Tyr230 and therefore two potential autoglucosylation sites. Glucosylation of Glg2p was studied using both matrix-assisted laser desorption ionization and electrospray quadrupole time of flight mass spectrometry. Glg2p, carrying a C-terminal (His(6)) tag, was produced in Escherichia coli and purified. By tryptic digestion and reversed phase chromatography a peptide (residues 219-246 of the complete Glg2p sequence) was isolated that contained 4-25 glucosyl residues. Following incubation of Glg2p with UDPglucose, more than 36 glucosyl residues were covalently bound to this peptide. Using a combination of cyanogen bromide cleavage of the protein backbone, enzymatic hydrolysis of glycosidic bonds and reversed phase chromatography, mono- and diglucosylated peptides having the sequence PNYGYQSSPAM were generated. MS/MS spectra revealed that glucosyl residues were attached to both Tyr232 and Tyr230 within the same peptide. The formation of the highly glucosylated eukaryotic Glg2p did not favour the bacterial glycogen accumulation. Under various experimental conditions Glg2p-producing cells accumulated approximately 30% less glycogen than a control transformed with a Glg2p lacking plasmid. The size distribution of the glycogen and extractable activities of several glycogen-related enzymes were essentially unchanged. As revealed by high performance anion exchange chromatography, the intracellular maltooligosaccharide pattern of the bacterial cells expressing the functional eukaryotic transgene was significantly altered. Thus, the eukaryotic glycogenin appears to be incompatible with the bacterial initiation of glycogen biosynthesis
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Albrecht, Tanja
A1  - Koch, Anke
A1  - Lode, Anja
A1  - Greve, Burkhard
A1  - Schneider-Mergener, Jens
A1  - Steup, Martin
T1  - Plastidic (Pho1-type) phosphorylase isoforms in potato (Solanum tuberosum L.) plants : expression analysis and immunochemical characterization
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Ale-Agha, Nosratollah
A1  - Bolay, Adrien
A1  - Braun, Uwe
A1  - Jage, Horst
A1  - Kummer, Volker
A1  - Lebeda, Ales
A1  - Piatek, Marcin
A1  - Shin, Hyeon-Dong
A1  - Zimmermannova-Pastircakova, Katarina
T1  - Erysiphe catalpae and E. elevata in Europe
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Ali, Tahir
A1  - Runge, Fabian
A1  - Dutbayev, Ayan
A1  - Schmuker, Angelika
A1  - Solovyeva, Irina
A1  - Nigrelli, Lisa
A1  - Buch, Ann-Katrin
A1  - Xia, Xiaojuan
A1  - Ploch, Sebastian
A1  - Orren, Ouria
A1  - Kummer, Volker
A1  - Paule, Juraj
A1  - Celik, Ali
A1  - Vakhrusheva, Ljudmila
A1  - Gabrielyan, Ivan
A1  - Thines, Marco
T1  - Microthlaspi erraticum (Jord.) T. Ali et Thines has a wide distribution, ranging from the Alps to the Tien Shan
JF  - Flora : morphology, distribution, functional ecology of plants
N2  - Microthlaspi is a predominantly Eurasian genus which also occurs in the northernmost parts of Africa (Maghreb). The most widespread species of the genus is M. perfoliatum, which can be found from Sweden to Algeria and from Portugal to China. The other species are thought to have much more confined distribution ranges, often covering only a few hundred kilometres. This is also believed for the diploid M. erraticum, which was recently re-appraised as a taxon independent from the tetra- to hexaploid M. perfoliatum. Previously, M. erraticum was believed to be present only in Central Europe, from the East of France to Slovenia. In order to gain a deeper understanding of the ecology, evolution and migration history of Microthlaspi it was the focus of the current study to investigate, if M. erraticum is present in habitats outside Central Europe, but with microclimates similar to Central Europe. It is demonstrated that M. erraticum is much more widespread than previously thought, while other lineages apart from M. perfoliatum s.str. and M. erraticum seem to have restricted distribution ranges. The latter species was observed from the Alps and their foreland, the Balkans, the mountainous areas around the Black Sea, Southern Siberia, as well as the Altai and Tien Shan mountains. This demonstrates a widespread occurrence of this easily-overlooked species. (C) 2016 Elsevier GmbH. All rights reserved.
KW  - Biogeography
KW  - Coluteocarpeae
KW  - Noccaea
KW  - Phylogeny
KW  - Species complex
KW  - Thlaspi perfoliatum
Y1  - 2016
U6  - https://doi.org/10.1016/j.flora.2016.09.008
SN  - 0367-2530
SN  - 1618-0585
VL  - 225
SP  - 76
EP  - 81
PB  - American Chemical Society
CY  - Jena
ER  - 
TY  - JOUR
A1  - Alker, Wiebke
A1  - Schwerdtle, Tanja
A1  - Schomburg, Lutz
A1  - Haase, Hajo
T1  - A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum
JF  - International journal of molecular sciences
N2  - Zinc is an essential trace element, making it crucial to have a reliable biomarker for evaluating an individual’s zinc status. The total serum zinc concentration, which is presently the most commonly used biomarker, is not ideal for this purpose, but a superior alternative is still missing. The free zinc concentration, which describes the fraction of zinc that is only loosely bound and easily exchangeable, has been proposed for this purpose, as it reflects the highly bioavailable part of serum zinc. This report presents a fluorescence-based method for determining the free zinc concentration in human serum samples, using the fluorescent probe Zinpyr-1. The assay has been applied on 154 commercially obtained human serum samples. Measured free zinc concentrations ranged from 0.09 to 0.42 nM with a mean of 0.22 ± 0.05 nM. It did not correlate with age or the total serum concentrations of zinc, manganese, iron or selenium. A negative correlation between the concentration of free zinc and total copper has been seen for sera from females. In addition, the free zinc concentration in sera from females (0.21 ± 0.05 nM) was significantly lower than in males (0.23 ± 0.06 nM). The assay uses a sample volume of less than 10 µL, is rapid and cost-effective and allows us to address questions regarding factors influencing the free serum zinc concentration, its connection with the body’s zinc status, and its suitability as a future biomarker for an individual’s zinc status.
KW  - zinc
KW  - free zinc
KW  - serum
KW  - biomarker
KW  - fluorescent probe
KW  - Zinypr-1
Y1  - 2019
U6  - https://doi.org/10.3390/ijms20164006
SN  - 1661-6596
SN  - 1422-0067
VL  - 20
IS  - 16
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Allan, Eric
A1  - Bossdorf, Oliver
A1  - Dormann, Carsten F.
A1  - Prati, Daniel
A1  - Gossner, Martin M.
A1  - Tscharntke, Teja
A1  - Blüthgen, Nico
A1  - Bellach, Michaela
A1  - Birkhofer, Klaus
A1  - Boch, Steffen
A1  - Böhm, Stefan
A1  - Börschig, Carmen
A1  - Chatzinotas, Antonis
A1  - Christ, Sabina
A1  - Daniel, Rolf
A1  - Diekötter, Tim
A1  - Fischer, Christiane
A1  - Friedl, Thomas
A1  - Glaser, Karin
A1  - Hallmann, Christine
A1  - Hodac, Ladislav
A1  - Hölzel, Norbert
A1  - Jung, Kirsten
A1  - Klein, Alexandra Maria
A1  - Klaus, Valentin H.
A1  - Kleinebecker, Till
A1  - Krauss, Jochen
A1  - Lange, Markus
A1  - Morris, E. Kathryn
A1  - Müller, Jörg
A1  - Nacke, Heiko
A1  - Pasalic, Esther
A1  - Rillig, Matthias C.
A1  - Rothenwoehrer, Christoph
A1  - Schally, Peter
A1  - Scherber, Christoph
A1  - Schulze, Waltraud X.
A1  - Socher, Stephanie A.
A1  - Steckel, Juliane
A1  - Steffan-Dewenter, Ingolf
A1  - Türke, Manfred
A1  - Weiner, Christiane N.
A1  - Werner, Michael
A1  - Westphal, Catrin
A1  - Wolters, Volkmar
A1  - Wubet, Tesfaye
A1  - Gockel, Sonja
A1  - Gorke, Martin
A1  - Hemp, Andreas
A1  - Renner, Swen C.
A1  - Schöning, Ingo
A1  - Pfeiffer, Simone
A1  - König-Ries, Birgitta
A1  - Buscot, Francois
A1  - Linsenmair, Karl Eduard
A1  - Schulze, Ernst-Detlef
A1  - Weisser, Wolfgang W.
A1  - Fischer, Markus
T1  - Interannual variation in land-use intensity enhances grassland multidiversity
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - Although temporal heterogeneity is a well-accepted driver of biodiversity, effects of interannual variation in land-use intensity (LUI) have not been addressed yet. Additionally, responses to land use can differ greatly among different organisms; therefore, overall effects of land-use on total local biodiversity are hardly known. To test for effects of LUI (quantified as the combined intensity of fertilization, grazing, and mowing) and interannual variation in LUI (SD in LUI across time), we introduce a unique measure of whole-ecosystem biodiversity, multidiversity. This synthesizes individual diversity measures across up to 49 taxonomic groups of plants, animals, fungi, and bacteria from 150 grasslands. Multidiversity declined with increasing LUI among grasslands, particularly for rarer species and aboveground organisms, whereas common species and belowground groups were less sensitive. However, a high level of interannual variation in LUI increased overall multidiversity at low LUI and was even more beneficial for rarer species because it slowed the rate at which the multidiversity of rare species declined with increasing LUI. In more intensively managed grasslands, the diversity of rarer species was, on average, 18% of the maximum diversity across all grasslands when LUI was static over time but increased to 31% of the maximum when LUI changed maximally over time. In addition to decreasing overall LUI, we suggest varying LUI across years as a complementary strategy to promote biodiversity conservation.
KW  - biodiversity loss
KW  - agricultural grasslands
KW  - Biodiversity Exploratories
Y1  - 2014
U6  - https://doi.org/10.1073/pnas.1312213111
SN  - 0027-8424
VL  - 111
IS  - 1
SP  - 308
EP  - 313
PB  - National Acad. of Sciences
CY  - Washington
ER  - 
TY  - JOUR
A1  - Allan, Eric
A1  - Manning, Pete
A1  - Alt, Fabian
A1  - Binkenstein, Julia
A1  - Blaser, Stefan
A1  - Blüthgen, Nico
A1  - Böhm, Stefan
A1  - Grassein, Fabrice
A1  - Hölzel, Norbert
A1  - Klaus, Valentin H.
A1  - Kleinebecker, Till
A1  - Morris, E. Kathryn
A1  - Oelmann, Yvonne
A1  - Prati, Daniel
A1  - Renner, Swen C.
A1  - Rillig, Matthias C.
A1  - Schaefer, Martin
A1  - Schloter, Michael
A1  - Schmitt, Barbara
A1  - Schöning, Ingo
A1  - Schrumpf, Marion
A1  - Solly, Emily
A1  - Sorkau, Elisabeth
A1  - Steckel, Juliane
A1  - Steffen-Dewenter, Ingolf
A1  - Stempfhuber, Barbara
A1  - Tschapka, Marco
A1  - Weiner, Christiane N.
A1  - Weisser, Wolfgang W.
A1  - Werner, Michael
A1  - Westphal, Catrin
A1  - Wilcke, Wolfgang
A1  - Fischer, Markus
T1  - Land use intensification alters ecosystem multifunctionality via loss of biodiversity and changes to functional composition
JF  - Ecology letters
N2  - Global change, especially land-use intensification, affects human well-being by impacting the delivery of multiple ecosystem services (multifunctionality). However, whether biodiversity loss is a major component of global change effects on multifunctionality in real-world ecosystems, as in experimental ones, remains unclear. Therefore, we assessed biodiversity, functional composition and 14 ecosystem services on 150 agricultural grasslands differing in land-use intensity. We also introduce five multifunctionality measures in which ecosystem services were weighted according to realistic land-use objectives. We found that indirect land-use effects, i.e. those mediated by biodiversity loss and by changes to functional composition, were as strong as direct effects on average. Their strength varied with land-use objectives and regional context. Biodiversity loss explained indirect effects in a region of intermediate productivity and was most damaging when land-use objectives favoured supporting and cultural services. In contrast, functional composition shifts, towards fast-growing plant species, strongly increased provisioning services in more inherently unproductive grasslands.
KW  - Biodiversity-ecosystem functioning
KW  - ecosystem services
KW  - global change
KW  - land use
KW  - multifunctionality
Y1  - 2015
U6  - https://doi.org/10.1111/ele.12469
SN  - 1461-023X
SN  - 1461-0248
VL  - 18
IS  - 8
SP  - 834
EP  - 843
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Allan, Eric
A1  - Weisser, Wolfgang W.
A1  - Fischer, Markus
A1  - Schulze, Ernst-Detlef
A1  - Weigelt, Alexandra
A1  - Roscher, Christiane
A1  - Baade, Jussi
A1  - Barnard, Romain L.
A1  - Bessler, Holger
A1  - Buchmann, Nina
A1  - Ebeling, Anne
A1  - Eisenhauer, Nico
A1  - Engels, Christof
A1  - Fergus, Alexander J. F.
A1  - Gleixner, Gerd
A1  - Gubsch, Marlen
A1  - Halle, Stefan
A1  - Klein, Alexandra Maria
A1  - Kertscher, Ilona
A1  - Kuu, Annely
A1  - Lange, Markus
A1  - Le Roux, Xavier
A1  - Meyer, Sebastian T.
A1  - Migunova, Varvara D.
A1  - Milcu, Alexandru
A1  - Niklaus, Pascal A.
A1  - Oelmann, Yvonne
A1  - Pasalic, Esther
A1  - Petermann, Jana S.
A1  - Poly, Franck
A1  - Rottstock, Tanja
A1  - Sabais, Alexander C. W.
A1  - Scherber, Christoph
A1  - Scherer-Lorenzen, Michael
A1  - Scheu, Stefan
A1  - Steinbeiss, Sibylle
A1  - Schwichtenberg, Guido
A1  - Temperton, Vicky
A1  - Tscharntke, Teja
A1  - Voigt, Winfried
A1  - Wilcke, Wolfgang
A1  - Wirth, Christian
A1  - Schmid, Bernhard
T1  - A comparison of the strength of biodiversity effects across multiple functions
JF  - Oecologia
N2  - In order to predict which ecosystem functions are most at risk from biodiversity loss, meta-analyses have generalised results from biodiversity experiments over different sites and ecosystem types. In contrast, comparing the strength of biodiversity effects across a large number of ecosystem processes measured in a single experiment permits more direct comparisons. Here, we present an analysis of 418 separate measures of 38 ecosystem processes. Overall, 45 % of processes were significantly affected by plant species richness, suggesting that, while diversity affects a large number of processes not all respond to biodiversity. We therefore compared the strength of plant diversity effects between different categories of ecosystem processes, grouping processes according to the year of measurement, their biogeochemical cycle, trophic level and compartment (above- or belowground) and according to whether they were measures of biodiversity or other ecosystem processes, biotic or abiotic and static or dynamic. Overall, and for several individual processes, we found that biodiversity effects became stronger over time. Measures of the carbon cycle were also affected more strongly by plant species richness than were the measures associated with the nitrogen cycle. Further, we found greater plant species richness effects on measures of biodiversity than on other processes. The differential effects of plant diversity on the various types of ecosystem processes indicate that future research and political effort should shift from a general debate about whether biodiversity loss impairs ecosystem functions to focussing on the specific functions of interest and ways to preserve them individually or in combination.
KW  - Bottom-up effects
KW  - Carbon cycling
KW  - Ecological synthesis
KW  - Ecosystem processes
KW  - Grasslands
KW  - Jena experiment
KW  - Nitrogen cycling
Y1  - 2013
U6  - https://doi.org/10.1007/s00442-012-2589-0
SN  - 0029-8549
VL  - 173
IS  - 1
SP  - 223
EP  - 237
PB  - Springer
CY  - New York
ER  - 
TY  - JOUR
A1  - Allhoff, Korinna Theresa
A1  - Ritterskamp, Daniel
A1  - Rall, Björn C.
A1  - Drossel, Barbara
A1  - Guill, Christian
T1  - Evolutionary food web model based on body masses gives realistic networks with permanent species turnover
JF  - Scientific reports
N2  - The networks of predator-prey interactions in ecological systems are remarkably complex, but nevertheless surprisingly stable in terms of long term persistence of the system as a whole. In order to understand the mechanism driving the complexity and stability of such food webs, we developed an eco-evolutionary model in which new species emerge as modifications of existing ones and dynamic ecological interactions determine which species are viable. The food-web structure thereby emerges from the dynamical interplay between speciation and trophic interactions. The proposed model is less abstract than earlier evolutionary food web models in the sense that all three evolving traits have a clear biological meaning, namely the average body mass of the individuals, the preferred prey body mass, and the width of their potential prey body mass spectrum. We observed networks with a wide range of sizes and structures and high similarity to natural food webs. The model networks exhibit a continuous species turnover, but massive extinction waves that affect more than 50% of the network are not observed.
Y1  - 2015
U6  - https://doi.org/10.1038/srep10955
SN  - 2045-2322
VL  - 5
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Allu, Annapurna Devi
A1  - Brotman, Yariv
A1  - Xue, Gang-Ping
A1  - Balazadeh, Salma
T1  - Transcription factor ANAC032 modulates JA/SA signalling in response to Pseudomonas syringae infection
JF  - EMBO reports
N2  - Responses to pathogens, including host transcriptional reprogramming, require partially antagonistic signalling pathways dependent on the phytohormones salicylic (SA) and jasmonic (JA) acids. However, upstream factors modulating the interplay of these pathways are not well characterized. Here, we identify the transcription factor ANAC032 from Arabidopsis thaliana as one such regulator in response to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst). ANAC032 directly represses MYC2 activation upon Pst attack, resulting in blockage of coronatine-mediated stomatal reopening which restricts entry of bacteria into plant tissue. Furthermore, ANAC032 activates SA signalling by repressing NIMIN1, a key negative regulator of SA-dependent defence. Finally, ANAC032 reduces expression of JA-responsive genes, including PDF1.2A. Thus, ANAC032 enhances resistance to Pst by generating an orchestrated transcriptional output towards key SA- and JA-signalling genes coordinated through direct binding of ANAC032 to the MYC2, NIMIN1 and PDF1.2A promoters.
KW  - Arabidopsis
KW  - jasmonic acid
KW  - pathogens
KW  - salicylic acid
KW  - transcription factor
Y1  - 2016
U6  - https://doi.org/10.15252/embr.201642197
SN  - 1469-221X
SN  - 1469-3178
VL  - 17
SP  - 1578
EP  - 1589
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Allu, Annapurna Devi
A1  - Simancas, Barbara
A1  - Balazadeh, Salma
A1  - Munne-Bosch, Sergi
T1  - Defense-Related Transcriptional Reprogramming in Vitamin E-Deficient Arabidopsis Mutants Exposed to Contrasting Phosphate Availability
JF  - Frontiers in plant science
N2  - Vitamin E inhibits the propagation of lipid peroxidation and helps protecting photosystem II from photoinhibition, but little is known about its possible role in plant response to Pi availability. Here, we aimed at examining the effect of vitamin E deficiency in Arabidopsis thaliana vte mutants on phytohormone contents and the expression of transcription factors in plants exposed to contrasting Pi availability. Plants were subjected to two doses of Pi, either unprimed (controls) or previously exposed to low Pi (primed). In the wild type, alpha-tocopherol contents increased significantly in response to repeated periods of low Pi, which was paralleled by increased growth, indicative of a priming effect. This growth-stimulating effect was, however, abolished in vte mutants. Hormonal profiling revealed significant effects of Pi availability, priming and genotype on the contents of jasmonates and salicylates; remarkably, vte mutants showed enhanced accumulation of both hormones under low Pi. Furthermore, expression profiling of 1,880 transcription factors by qRT-PCR revealed a pronounced effect of priming on the transcript levels of 45 transcription factors mainly associated with growth and stress in wild-type plants in response to low Pi availability; while distinct differences in the transcriptional response were detected in vte mutants. We conclude that alpha-tocopherol plays a major role in the response of plants to Pi availability not only by protecting plants from photo-oxidative stress, but also by exerting a control over growth-and defense-related transcriptional reprogramming and hormonal modulation.
KW  - antioxidants
KW  - photosystem II
KW  - plastochromanol-8
KW  - priming
KW  - retrograde signaling
KW  - tocochromanols
KW  - vitamin E
Y1  - 2017
U6  - https://doi.org/10.3389/fpls.2017.01396
SN  - 1664-462X
VL  - 8
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Allu, Annapurna Devi
A1  - Soja, Aleksandra Maria
A1  - Wu, Anhui
A1  - Szymanski, Jedrzej
A1  - Balazadeh, Salma
T1  - Salt stress and senescence: identification of cross-talk regulatory components
JF  - Journal of experimental botany
N2  - Leaf senescence is an active process with a pivotal impact on plant productivity. It results from extensive signalling cross-talk coordinating environmental factors with intrinsic age-related mechanisms. Although many studies have shown that leaf senescence is affected by a range of external parameters, knowledge about the regulatory systems that govern the interplay between developmental programmes and environmental stress is still vague. Salinity is one of the most important environmental stresses that promote leaf senescence and thus affect crop yield. Improving salt tolerance by avoiding or delaying senescence under stress will therefore play an important role in maintaining high agricultural productivity. Experimental evidence suggests that hydrogen peroxide (H2O2) functions as a common signalling molecule in both developmental and salt-induced leaf senescence. In this study, microarray-based gene expression profiling on Arabidopsis thaliana plants subjected to long-term salinity stress to induce leaf senescence was performed, together with co-expression network analysis for H2O2-responsive genes that are mutually up-regulated by salt induced-and developmental leaf senescence. Promoter analysis of tightly co-expressed genes led to the identification of seven cis-regulatory motifs, three of which were known previously, namely CACGTGT and AAGTCAA, which are associated with reactive oxygen species (ROS)-responsive genes, and CCGCGT, described as a stress-responsive regulatory motif, while the others, namely ACGCGGT, AGCMGNC, GMCACGT, and TCSTYGACG were not characterized previously. These motifs are proposed to be novel elements involved in the H2O2-mediated control of gene expression during salinity stress-triggered and developmental senescence, acting through upstream transcription factors that bind to these sites.
KW  - Arabidopsis
KW  - hydrogen peroxide
KW  - longevity
KW  - reactive oxygen species
KW  - salt stress
KW  - senescence
KW  - signal cross-talk
KW  - transcription factor
Y1  - 2014
U6  - https://doi.org/10.1093/jxb/eru173
SN  - 0022-0957
SN  - 1460-2431
VL  - 65
IS  - 14
SP  - 3993
EP  - 4008
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Almathen, Faisal
A1  - Charruau, Pauline
A1  - Mohandesan, Elmira
A1  - Mwacharo, Joram M.
A1  - Orozco-terWengel, Pablo
A1  - Pitt, Daniel
A1  - Abdussamad, Abdussamad M.
A1  - Uerpmann, Margarethe
A1  - Uerpmann, Hans-Peter
A1  - De Cupere, Bea
A1  - Magee, Peter
A1  - Alnaqeeb, Majed A.
A1  - Salim, Bashir
A1  - Raziq, Abdul
A1  - Dessie, Tadelle
A1  - Abdelhadi, Omer M.
A1  - Banabazi, Mohammad H.
A1  - Al-Eknah, Marzook
A1  - Walzer, Chris
A1  - Fayer, Bernard
A1  - Hofreiter, Michael
A1  - Peters, Joris
A1  - Hanotte, Olivier
A1  - Burger, Pamela A.
T1  - Ancient and modern DNA reveal dynamics of domestication and cross-continental dispersal of the dromedary
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - Dromedaries have been fundamental to the development of human societies in arid landscapes and for long-distance trade across hostile hot terrains for 3,000 y. Today they continue to be an important livestock resource in marginal agro-ecological zones. However, the history of dromedary domestication and the influence of ancient trading networks on their genetic structure have remained elusive. We combined ancient DNA sequences of wild and early-domesticated dromedary samples from arid regions with nuclear microsatellite and mitochondrial genotype information from 1,083 extant animals collected across the species’ range. We observe little phylogeographic signal in the modern population, indicative of extensive gene flow and virtually affecting all regions except East Africa, where dromedary populations have remained relatively isolated. In agreement with archaeological findings, we identify wild dromedaries from the southeast Arabian Peninsula among the founders of the domestic dromedary gene pool. Approximate Bayesian computations further support the “restocking from the wild” hypothesis, with an initial domestication followed by introgression from individuals from wild, now-extinct populations. Compared with other livestock, which show a long history of gene flow with their wild ancestors, we find a high initial diversity relative to the native distribution of the wild ancestor on the Arabian Peninsula and to the brief coexistence of early-domesticated and wild individuals. This study also demonstrates the potential to retrieve ancient DNA sequences from osseous remains excavated in hot and dry desert environments.
KW  - anthropogenic admixture
KW  - Camelus dromedarius
KW  - demographic history
KW  - paleogenetics
KW  - wild dromedary
Y1  - 2016
U6  - https://doi.org/10.1073/pnas.1519508113
SN  - 0027-8424
VL  - 113
SP  - 6707
EP  - 6712
PB  - National Acad. of Sciences
CY  - Washington
ER  - 
TY  - JOUR
A1  - Alseekh, Saleh
A1  - Tohge, Takayuki
A1  - Wendenberg, Regina
A1  - Scossa, Federico
A1  - Omranian, Nooshin
A1  - Li, Jie
A1  - Kleessen, Sabrina
A1  - Giavalisco, Patrick
A1  - Pleban, Tzili
A1  - Müller-Röber, Bernd
A1  - Zamir, Dani
A1  - Nikoloski, Zoran
A1  - Fernie, Alisdair R.
T1  - Identification and Mode of Inheritance of Quantitative Trait Loci for Secondary Metabolite Abundance in Tomato
JF  - The plant cell
N2  - A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgression lines. Heritability analyses revealed that mQTLs of secondary metabolism were less affected by environment than mQTLs of primary metabolism. Network analysis allowed us to assess the interconnectivity of primary and secondary metabolism as well as to compare and contrast their respective associations with morphological traits. Additionally, we applied a recently established real-time quantitative PCR platform to gain insight into transcriptional control mechanisms of a subset of the mQTLs, including those for hydroxycinnamates, acyl-sugar, naringenin chalcone, and a range of glycoalkaloids. Intriguingly, many of these compounds displayed a dominant-negative mode of inheritance, which is contrary to the conventional wisdom that secondary metabolite contents decreased on domestication. We additionally performed an exemplary evaluation of two candidate genes for glycolalkaloid mQTLs via the use of virus-induced gene silencing. The combined data of this study were compared with previous results on primary metabolism obtained from the same material and to other studies of natural variance of secondary metabolism.
Y1  - 2015
U6  - https://doi.org/10.1105/tpc.114.132266
SN  - 1040-4651
SN  - 1532-298X
VL  - 27
IS  - 3
SP  - 485
EP  - 512
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Alter, S. Elizabeth
A1  - Meyer, Matthias
A1  - Post, Klaas
A1  - Czechowski, Paul
A1  - Gravlund, Peter
A1  - Gaines, Cork
A1  - Rosenbaum, Howard C.
A1  - Kaschner, Kristin
A1  - Turvey, Samuel T.
A1  - van der Plicht, Johannes
A1  - Shapiro, Beth
A1  - Hofreiter, Michael
T1  - Climate impacts on transocean dispersal and habitat in gray whales from the Pleistocene to 2100
JF  - Molecular ecology
N2  - Arctic animals face dramatic habitat alteration due to ongoing climate change. Understanding how such species have responded to past glacial cycles can help us forecast their response to today's changing climate. Gray whales are among those marine species likely to be strongly affected by Arctic climate change, but a thorough analysis of past climate impacts on this species has been complicated by lack of information about an extinct population in the Atlantic. While little is known about the history of Atlantic gray whales or their relationship to the extant Pacific population, the extirpation of the Atlantic population during historical times has been attributed to whaling. We used a combination of ancient and modern DNA, radiocarbon dating and predictive habitat modelling to better understand the distribution of gray whales during the Pleistocene and Holocene. Our results reveal that dispersal between the Pacific and Atlantic was climate dependent and occurred both during the Pleistocene prior to the last glacial period and the early Holocene immediately following the opening of the Bering Strait. Genetic diversity in the Atlantic declined over an extended interval that predates the period of intensive commercial whaling, indicating this decline may have been precipitated by Holocene climate or other ecological causes. These first genetic data for Atlantic gray whales, particularly when combined with predictive habitat models for the year 2100, suggest that two recent sightings of gray whales in the Atlantic may represent the beginning of the expansion of this species' habitat beyond its currently realized range.
KW  - ancient DNA
KW  - climate change
KW  - last glacial maximum
KW  - marine mammal
Y1  - 2015
U6  - https://doi.org/10.1111/mec.13121
SN  - 0962-1083
SN  - 1365-294X
VL  - 24
IS  - 7
SP  - 1510
EP  - 1522
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Altintas, Zeynep
A1  - Takiden, Aref
A1  - Utesch, Tillmann
A1  - Mroginski, Maria A.
A1  - Schmid, Bianca
A1  - Scheller, Frieder W.
A1  - Süssmuth, Roderich D.
T1  - Integrated approaches toward high-affinity artificial protein binders obtained via computationally simulated epitopes for protein recognition
JF  - Advanced functional materials
N2  - Widely used diagnostic tools make use of antibodies recognizing targeted molecules, but additional techniques are required in order to alleviate the disadvantages of antibodies. Herein, molecular dynamic calculations are performed for the design of high affinity artificial protein binding surfaces for the recognition of neuron specific enolase (NSE), a known cancer biomarker. Computational simulations are employed to identify particularly stabile secondary structure elements. These epitopes are used for the subsequent molecular imprinting, where surface imprinting approach is applied. The molecular imprints generated with the calculated epitopes of greater stability (Cys-Ep1) show better binding properties than those of lower stability (Cys-Ep5). The average binding strength of imprints created with stabile epitopes is found to be around twofold and fourfold higher for the NSE derived peptide and NSE protein, respectively. The recognition of NSE is investigated in a wide concentration range, where high sensitivity (limit of detection (LOD) = 0.5 ng mL(-1)) and affinity (dissociation constant (K-d) = 5.3 x 10(-11)m) are achieved using Cys-Ep1 imprints reflecting the stable structure of the template molecules. This integrated approach employing stability calculations for the identification of stabile epitopes is expected to have a major impact on the future development of high affinity protein capturing binders.
KW  - artificial protein binders
KW  - cancer markers
KW  - computationally simulated epitopes
KW  - molecular imprinting
KW  - protein recognition
Y1  - 2019
U6  - https://doi.org/10.1002/adfm.201807332
SN  - 1616-301X
SN  - 1616-3028
VL  - 29
IS  - 15
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
T1  - Methodik der funktionellen Genomanalyse : wie mit Mikroarrays die Aktivität vieler Gene erfasst wird
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Basse, Christoph W.
A1  - Kerschbamer, Christine
A1  - Brustmann, Markus
A1  - Kahmann, Regine
T1  - Evidence for a Ustilago maydis steroid 5 alpha-reductase by functional expression in Arabidopsis det2-1 mutants
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Brandt, Stephan Peter
A1  - Kloska, Sebastian
A1  - Kehr, Julia
T1  - Using array hybridization to monitore gene expression at the single cell level
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Colebatch, G.
A1  - Kloska, Sebastian
A1  - Trevaskis, B.
A1  - Freund, S.
A1  - Udvardi, M. K.
T1  - Novel aspects of symbiotic nitrogen fixation uncovered by transcript profiling with cDNA arrays
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Fiehn, Oliver
A1  - Kloska, Sebastian
T1  - Integrated studies on plant biology using multiparallel techniques
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Koßmann, Jens
T1  - Photosynthesis and primary metabolism
Y1  - 2001
SN  - 1360-1385
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Müssig, Carsten
T1  - Brassinosteroid signaling in plants
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Müssig, Carsten
A1  - Fischer, Sabine
T1  - Brassinosteroid-regulated gene expression
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Narang, R. A.
T1  - Phosphate accquisition heterosis in Arabidopsis thaliana : a morphological and physiological analysis
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Schlüter, U.
A1  - Köpke, D.
A1  - Müssig, Carsten
T1  - Analysis of carbohydrate metabolism of CPD antisense plants and the brassinosteroid-deficient cbb1 mutant
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Schlüter, U.
A1  - Muschak, M.
A1  - Berger, Dieter
T1  - Photosynthetic performance of an Arabidopsis mutant with elevated stomatal density (sdd1-1) under different light regimes
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Schmid, K. J.
A1  - Sörensen, Rossleff T.
A1  - Stracke, R.
A1  - Törjek, Otto
A1  - Mitchel-Olds, T.
A1  - Weisshaar, Bernd
T1  - Large-scale identification and analysis of genome-wide single-nucleotide polymorphisms for mapping in Arabidopsis thaliana
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Altmann, Thomas
A1  - Taylor, Janet
A1  - King, Ross. D.
A1  - Fiehn, Oliver
T1  - Application of metabolomics to plant genotype discrimination using statistics and machine learning
Y1  - 2003
ER  -