TY  - GEN
A1  - Muiño, Jose M.
A1  - de Bruijn, Suzanne
A1  - Pajoro, Alice
A1  - Geuten, Koen
A1  - Vingron, Martin
A1  - Angenent, Gerco C.
A1  - Kaufmann, Kerstin
T1  - Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor
N2  - Flower development is controlled by the action of key regulatory transcription factors of the MADS-domain family. The function of these factors appears to be highly conserved among species based on mutant phenotypes. However, the conservation of their downstream processes is much less well understood, mostly because the evolutionary turnover and variation of their DNA-binding sites (BSs) among plant species have not yet been experimentally determined. Here, we performed comparative ChIP (chromatin immunoprecipitation)-seq experiments of the MADS-domain transcription factor SEPALLATA3 (SEP3) in two closely related Arabidopsis species: Arabidopsis thaliana and A. lyrata which have very similar floral organ morphology. We found that BS conservation is associated with DNA sequence conservation, the presence of the CArG-box BS motif and on the relative position of the BS to its potential target gene. Differences in genome size and structure can explain that SEP3 BSs in A. lyrata can be located more distantly to their potential target genes than their counterparts in A. thaliana. In A. lyrata, we identified transposition as a mechanism to generate novel SEP3 binding locations in the genome. Comparative gene expression analysis shows that the loss/gain of BSs is associated with a change in gene expression. In summary, this study investigates the evolutionary dynamics of DNA BSs of a floral key-regulatory transcription factor and explores factors affecting this phenomenon.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 261 
KW  - MADS-domain transcription factor
KW  - cis-regulatory evolution
KW  - plant development
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-96580
SP  - 1225
EP  - 1245
ER  - 
TY  - JOUR
A1  - Muiño, Jose M.
A1  - de Bruijn, Suzanne
A1  - Pajoro, Alice
A1  - Geuten, Koen
A1  - Vingron, Martin
A1  - Angenent, Gerco C.
A1  - Kaufmann, Kerstin
T1  - Evolution of DNA-Binding Sites of a Floral Master Regulatory Transcription Factor
JF  - Molecular biology and evolution : MBE
N2  - lower development is controlled by the action of key regulatory transcription factors of the MADS-domain family. The function of these factors appears to be highly conserved among species based on mutant phenotypes. However, the conservation of their downstream processes is much less well understood, mostly because the evolutionary turnover and variation of their DNA-binding sites (BSs) among plant species have not yet been experimentally determined. Here, we performed comparative ChIP (chromatin immunoprecipitation)-seq experiments of the MADS-domain transcription factor SEPALLATA3 (SEP3) in two closely related Arabidopsis species: Arabidopsis thaliana and A. lyrata which have very similar floral organ morphology. We found that BS conservation is associated with DNA sequence conservation, the presence of the CArG-box BS motif and on the relative position of the BS to its potential target gene. Differences in genome size and structure can explain that SEP3 BSs in A. lyrata can be located more distantly to their potential target genes than their counterparts in A. thaliana. In A. lyrata, we identified transposition as a mechanism to generate novel SEP3 binding locations in the genome. Comparative gene expression analysis shows that the loss/gain of BSs is associated with a change in gene expression. In summary, this study investigates the evolutionary dynamics of DNA BSs of a floral key-regulatory transcription factor and explores factors affecting this phenomenon.
KW  - MADS-domain transcription factor
KW  - plant development
KW  - cis-regulatory evolution
Y1  - 2015
U6  - https://doi.org/10.1093/molbev/msv210
SN  - 1537-1719
SN  - 0737-4038
VL  - 33
IS  - 1
PB  - Oxford University Press
CY  - Oxford
ER  -