TY  - JOUR
A1  - Li, Zhengdong
A1  - Xu, Xun
A1  - Wang, Weiwei
A1  - Kratz, Karl
A1  - Sun, Xianlei
A1  - Zou, Jie
A1  - Deng, Zijun
A1  - Jung, Friedrich Wilhelm
A1  - Gossen, Manfred
A1  - Ma, Nan
A1  - Lendlein, Andreas
T1  - Modulation of the mesenchymal stem cell migration capacity via preconditioning with topographic microstructure
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - Controlling mesenchymal stem cells (MSCs) behavior is necessary to fully exploit their therapeutic potential. Various approaches are employed to effectively influence the migration capacity of MSCs. Here, topographic microstructures with different microscale roughness were created on polystyrene (PS) culture vessel surfaces as a feasible physical preconditioning strategy to modulate MSC migration. By analyzing trajectories of cells migrating after reseeding, we demonstrated that the mobilization velocity of human adipose derived mesenchymal stem cells (hADSCs) could be promoted by and persisted after brief preconditioning with the appropriate microtopography. Moreover, the elevated activation levels of focal adhesion kinase (FAK) and mitogen-activated protein kinase (MAPK) in hADSCs were also observed during and after the preconditioning process. These findings underline the potential enhancement of in vivo therapeutic efficacy in regenerative medicine via transplantation of topographic microstructure preconditioned stem cells.
KW  - Mesenchymal stem cells
KW  - precondition
KW  - microstructure
KW  - migration
KW  - FAK-MAPK
Y1  - 2017
U6  - https://doi.org/10.3233/CH-179208
SN  - 1386-0291
SN  - 1875-8622
VL  - 67
SP  - 267
EP  - 278
PB  - IOS Press
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Krüger-Genge, Anne
A1  - Dietze, Stefanie
A1  - Yan, Wan
A1  - Liu, Yue
A1  - Fang, Liang
A1  - Kratz, Karl
A1  - Lendlein, Andreas
A1  - Jung, Friedrich
T1  - Endothelial cell migration, adhesion and proliferation on different polymeric substrates
JF  - Clinical hemorheology and microcirculation : blood flow and vessels
N2  - BACKGROUND: The formation of a functionally-confluent endothelial cell (EC) monolayer affords proliferation of EC, which only happens in case of appropriate migratory activity. AIM OF THE STUDY: The migratory pathway of human umbilical endothelial cells (HUVEC) was investigated on different polymeric substrates. MATERIAL AND METHODS: Surface characterization of the polymers was performed by contact angle measurements and atomic force microscopy under wet conditions. 30,000 HUVEC per well were seeded on polytetrafluoroethylene (PTFE) (theta(adv) = 119 degrees +/- 2 degrees), on low-attachment plate LAP (theta(adv) = 28 degrees +/- 2 degrees) and on polystyrene based tissue culture plates (TCP, theta(adv) = 22 degrees +/- 1 degrees). HUVEC tracks (trajectories) were recorded by time lapse microscopy and the euclidean distance (straight line between starting and end point), the total distance and the velocities of HUVEC not leaving the vision field were determined. RESULTS: On PTFE, 42 HUVEC were in the vision field directly after seeding. The mean length of single migration steps (SML) was 6.1 +/- 5.2 mu m, the mean velocity (MV) 0.40 +/- 0.3 mu m.min(-1) and the complete length of the trajectory (LT) was 710 +/- 440 mu m. On TCP 82 HUVEC were in the vision field subsequent to seeding. The LT was 840 +/- 550 mu m, the SML 6.1 +/- 5.2 mu m and the MV 0.44 +/- 0.3 mu m.min(-1). The trajectories on LAP differed significantly in respect to SML (2.4 +/- 3.9 mu m, p <0.05), the MV (0.16 +/- 0.3 mu m.min(-1), p <0.05) and the LT (410 +/- 300 mu m, p <0.05), compared to PTFE and TCP. Solely on TCP a nearly confluent EC monolayer developed after three days. While on TCP diffuse signals of vinculin were found over the whole basal cell surface organizing the binding of the cells by focal adhesions, on PTFE vinculin was merely arranged at the cell rims, and on the hydrophilic material (LAP) no focal adhesions were found. CONCLUSION: The study revealed that the wettability of polymers affected not only the initial adherence but also the migration of EC, which is of importance for the proliferation and ultimately the endothelialization of polymer-based biomaterials.
KW  - Endothelial cells
KW  - migration
KW  - polymer-based biomaterials
KW  - cytokine release
Y1  - 2019
U6  - https://doi.org/10.3233/CH-189317
SN  - 1386-0291
SN  - 1875-8622
VL  - 70
IS  - 4
SP  - 511
EP  - 529
PB  - IOS Press
CY  - Amsterdam
ER  -