TY  - JOUR
A1  - Shahnejat-Bushehri, Sara
A1  - Allu, Annapurna Devi
A1  - Mehterov, Nikolay
A1  - Thirumalaikumar, Venkatesh P.
A1  - Alseekh, Saleh
A1  - Fernie, Alisdair R.
A1  - Mueller-Roeber, Bernd
A1  - Balazadeh, Salma
T1  - Arabidopsis NAC Transcription Factor JUNGBRUNNEN1 Exerts Conserved Control Over Gibberellin and Brassinosteroid Metabolism and Signaling Genes in Tomato
JF  - Frontiers in plant science
N2  - The Arabidopsis thaliana NAC transcription factor JUNGBRUNNEN1 (AtJUB1) regulates growth by directly repressing GA3ox1 and DWF4, two key genes involved in gibberellin (GA) and brassinosteroid (BR) biosynthesis, respectively, leading to GA and BR deficiency phenotypes. AtJUB1 also reduces the expression of PIF4, a bHLH transcription factor that positively controls cell elongation, while it stimulates the expression of DELLA genes, which are important repressors of growth. Here, we extend our previous findings by demonstrating that AtJUB1 induces similar GA and BR deficiency phenotypes and changes in gene expression when overexpressed in tomato (Solanum lycopersicum). Importantly, and in accordance with the growth phenotypes observed, AtJUB1 inhibits the expression of growth-supporting genes, namely the tomato orthologs of GA3ox1, DWF4 and PIF4, but activates the expression of DELLA orthologs, by directly binding to their promoters. Overexpression of AtJUB1 in tomato delays fruit ripening, which is accompanied by reduced expression of several ripeningrelated genes, and leads to an increase in the levels of various amino acids (mostly proline, beta-alanine, and phenylalanine), gamma-aminobutyric acid (GABA), and major organic acids including glutamic acid and aspartic acid. The fact that AtJUB1 exerts an inhibitory effect on the GA/BR biosynthesis and PIF4 genes but acts as a direct activator of DELLA genes in both, Arabidopsis and tomato, strongly supports the model that the molecular constituents of the JUNGBRUNNEN1 growth control module are considerably conserved across species.
KW  - Arabidopsis
KW  - tomato
KW  - fruit
KW  - growth
KW  - transcription factor
KW  - gibberellic acid
KW  - brassinosteroid
KW  - DELLA proteins
Y1  - 2017
U6  - https://doi.org/10.3389/fpls.2017.00214
SN  - 1664-462X
VL  - 8
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  - 
TY  - JOUR
A1  - Thirumalaikumar, Venkatesh P.
A1  - Devkar, Vikas
A1  - Mehterov, Nikolay
A1  - Ali, Shawkat
A1  - Ozgur, Rengin
A1  - Turkan, Ismail
A1  - Müller-Röber, Bernd
A1  - Balazadeh, Salma
T1  - NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato
JF  - Plant Biotechnology Journal
N2  - Water deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell-damaging reactive oxygen species and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus-induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2O2) levels and a decrease in the expression of various drought-responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2 and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress-related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato.
KW  - Arabidopsis
KW  - tomato
KW  - transcription factor
KW  - drought
KW  - reactive oxygen species
KW  - DELLA
Y1  - 2017
U6  - https://doi.org/10.1111/pbi.12776
SN  - 1467-7644
SN  - 1467-7652
VL  - 16
IS  - 2
SP  - 354
EP  - 366
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Awais, Muhammad
A1  - Ahmad, Rafiq
A1  - Khan, Nadeem
A1  - Garapati, Prashanth
A1  - Shahzad, Muhammad
A1  - Afroz, Amber
A1  - Rashid, Umer
A1  - Khan, Sabaz Ali
T1  - Transformation of tomato variety rio grande with drought resistant transcription factor gene ATAF1 and its molecular analysis
JF  - Pakistan Journal of Botany
N2  - Tomato (Solanum lycopersicum L.) being an important vegetable is cultivated and used throughout the world. It not only contributes in fulfilling the basic nutritional requirements of the human body but also has many health benefits due to its rich biochemical composition. However, its production at large scale is hampered by many limiting factors such as biotic and abiotic stresses. Among the different abiotic stresses, drought poses drastic impact on tomato yield. Drought stress is genetically regulated by many transcription factors that not only regulate the stress responsive mechanism but also facilitate the growth and development of tomato plants. NAC is an important stress related transcription factor genes family, and the ATAF1 gene, a member of this family, is involved in ABA signaling and stress response. In this study, tomato variety Rio Drande was transformed with drought resistant ATAF1 gene via Agrobacterium mediated gene transformation method. The ATAF1 gene was first cloned in the pK7WFG2 vector having kanamycin selectable marker and then it was introduced in the Agrobacterium tumefaciens strain GV3101 through heat shock method. The tomato cotyledon and hypocotyl ex-plants of variety "Rio Ggrande" were cultured on callus induction medium (MS + 2.5 mg/L IAA + 2 mg/L BAP). The calli were then infected with Agrobacterium tumefaciens strain GV3101 containing ATAF1 gene and selection was carried out on the kanamycin selectable medium (MS + 100 mg/L Kan), and were regenerated on MS medium with 1 mg/L IAA + 1 mg/L BAP. Out of 216 putative transformed calli, 13 calli were able to regenerate on the selection medium. Of the 13 calli, three transgenic tomato plantlets were recovered, and these were confirmed through PCR analysis for the presence of 432 bp fragment of ATAF1 gene. The transformation protocol reported here can be used to generate drought resistant tomato plants in future.
KW  - Agrobacterium tumefaciens
KW  - drought stress
KW  - NAC transcription factor ATAF1
KW  - plant transformation
KW  - Rio Grande
KW  - tomato
Y1  - 2018
SN  - 0556-3321
SN  - 2070-3368
VL  - 50
IS  - 5
SP  - 1811
EP  - 1820
PB  - Pakistan botanic soc
CY  - Karachi
ER  - 
TY  - JOUR
A1  - Ma, Xuemin
A1  - Balazadeh, Salma
A1  - Mueller-Roeber, Bernd
T1  - Tomato fruit ripening factor NOR controls leaf senescence
JF  - Journal of experimental botany
N2  - NAC transcription factors (TFs) are important regulators of expressional reprogramming during plant development, stress responses, and leaf senescence. NAC TFs also play important roles in fruit ripening. In tomato (Solanum lycopersicum), one of the best characterized NACs involved in fruit ripening is NON-RIPENING (NOR), and the non-ripening (nor) mutation has been widely used to extend fruit shelf life in elite varieties. Here, we show that NOR additionally controls leaf senescence. Expression of NOR increases with leaf age, and developmental as well as dark-induced senescence are delayed in the nor mutant, while overexpression of NOR promotes leaf senescence. Genes associated with chlorophyll degradation as well as senescence-associated genes (SAGs) show reduced and elevated expression, respectively, in nor mutants and NOR overexpressors. Overexpression of NOR also stimulates leaf senescence in Arabidopsis thaliana. In tomato, NOR supports senescence by directly and positively regulating the expression of several senescence-associated genes including, besides others, SlSAG15 and SlSAG113, SlSGR1, and SlYLS4. Finally, we find that another senescence control NAC TF, namely SlNAP2, acts upstream of NOR to regulate its expression. Our data support a model whereby NAC TFs have often been recruited by higher plants for both the control of leaf senescence and fruit ripening.
KW  - Aging
KW  - leaf
KW  - NAC
KW  - non-ripening
KW  - NOR
KW  - senescence
KW  - tomato
KW  - transcription factor
Y1  - 2019
U6  - https://doi.org/10.1093/jxb/erz098
SN  - 0022-0957
SN  - 1460-2431
VL  - 70
IS  - 10
SP  - 2727
EP  - 2740
PB  - Oxford Univ. Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Lisso, Janina
A1  - Altmann, Thomas
A1  - Müssig, Carsten
T1  - Metabolic changes in fruits of the tomato d(x) mutant
JF  - Phytochemistry : an international journal of plant biochemistry
KW  - Solanum lycopersicum
KW  - Solanaceae
KW  - tomato
KW  - brassinosteroid
KW  - primary metabolism
KW  - fruit
Y1  - 2006
U6  - https://doi.org/10.1016/j.phytochem.2006.07.008
SN  - 0031-9422
VL  - 67
IS  - 20
SP  - 2232
EP  - 2238
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - GEN
A1  - Thirumalaikumar, Venkatesh P.
A1  - Devkar, Vikas
A1  - Mehterov, Nikolay
A1  - Ali, Shawkat
A1  - Ozgur, Rengin
A1  - Turkan, Ismail
A1  - Müller-Röber, Bernd
A1  - Balazadeh, Salma
T1  - NAC transcription factor JUNGBRUNNEN1 enhances drought tolerance in tomato
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - Water deficit (drought stress) massively restricts plant growth and the yield of crops; reducing the deleterious effects of drought is therefore of high agricultural relevance. Drought triggers diverse cellular processes including the inhibition of photosynthesis, the accumulation of cell‐damaging reactive oxygen species and gene expression reprogramming, besides others. Transcription factors (TF) are central regulators of transcriptional reprogramming and expression of many TF genes is affected by drought, including members of the NAC family. Here, we identify the NAC factor JUNGBRUNNEN1 (JUB1) as a regulator of drought tolerance in tomato (Solanum lycopersicum). Expression of tomato JUB1 (SlJUB1) is enhanced by various abiotic stresses, including drought. Inhibiting SlJUB1 by virus‐induced gene silencing drastically lowers drought tolerance concomitant with an increase in ion leakage, an elevation of hydrogen peroxide (H2O2) levels and a decrease in the expression of various drought‐responsive genes. In contrast, overexpression of AtJUB1 from Arabidopsis thaliana increases drought tolerance in tomato, alongside with a higher relative leaf water content during drought and reduced H2O2 levels. AtJUB1 was previously shown to stimulate expression of DREB2A, a TF involved in drought responses, and of the DELLA genes GAI and RGL1. We show here that SlJUB1 similarly controls the expression of the tomato orthologs SlDREB1, SlDREB2 and SlDELLA. Furthermore, AtJUB1 directly binds to the promoters of SlDREB1, SlDREB2 and SlDELLA in tomato. Our study highlights JUB1 as a transcriptional regulator of drought tolerance and suggests considerable conservation of the abiotic stress‐related gene regulatory networks controlled by this NAC factor between Arabidopsis and tomato.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 568 
KW  - Arabidopsis
KW  - tomato
KW  - transcription factor
KW  - drought
KW  - reactive oxygen species
KW  - DELLA
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-423908
SN  - 1866-8372
IS  - 568
ER  - 
TY  - THES
A1  - Bäßler, Olivia
T1  - Identifizierung und Charakterisierung IgE- reaktiver Proteine in der Tomate (Lycopersicon esculentum)
T1  - Novel tomato allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation ; mass spectrometry and in silico epitope modelling
N2  - Zur Detektion neuer IgE- reaktiver Proteine wurde in dieser Arbeit ein zweidimensionales Proteintrennverfahren verwendet. Resultierende Proteinfraktionen wurden mithilfe von 18 tomatensensibiliesierten Patientenseren im Immunoblot getestet. Detektierte Proteine in der SDS-PAGE wurden mittels LC-MS/MS identifiziert. Dadurch konnten 2 Tomatensamenproteine, die im Immunoblot ein IgE- reaktives Signal zeigten eindeutig mittels Massenspektrometrie identifiziert werden. Diese Proteine sind Legumin und Vicilin. Durch Sequenzabgleich und Proteinstrukturmodellierung im Vergleich zu bereits bekannten Allergenen (Erdnuss und Cashewnuss), konnte eine hohe Homologie gezeigt werden.
N2  - For the detection of tomato allergens a multidimensional protein fractionation strategy and LC-MS/MS was used. Putative allergens were detected by IgE immunoblotting using sera from 18 adult tomato sensitised patients selected based on a positive history skin prick test and specific Immunglobulin (Ig) E levels. Two legumin- and vicilin- proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modelling indicates high homology between epitopes of known peanut and cashewnut allergens and the detected IgE-crossreactive tomato proteins.
KW  - Massenspektrometrie
KW  - Tomate
KW  - Nahrungsmittelallergie
KW  - Speicherproteine
KW  - mass spectrometry
KW  - tomato
KW  - food allergy
KW  - storage proteins
Y1  - 2008
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-26953
ER  -