TY  - GEN
A1  - Paijmans, Johanna L. A.
A1  - Barlow, Axel
A1  - Henneberger, Kirstin
A1  - Fickel, Jörns
A1  - Hofreiter, Michael
A1  - Foerste, Daniel W. G.
T1  - Ancestral mitogenome capture of the Southeast Asian banded linsang
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Utilising a reconstructed ancestral mitochondrial genome of a clade to design hybridisation capture baits can provide the opportunity for recovering mitochondrial sequences from all its descendent and even sister lineages. This approach is useful for taxa with no extant close relatives, as is often the case for rare or extinct species, and is a viable approach for the analysis of historical museum specimens. Asiatic linsangs (genus Prionodon) exemplify this situation, being rare Southeast Asian carnivores for which little molecular data is available. Using ancestral capture we recover partial mitochondrial genome sequences for seven banded linsangs (P. linsang) from historical specimens, representing the first intraspecific genetic dataset for this species. We additionally assemble a high quality mitogenome for the banded linsang using shotgun sequencing for time-calibrated phylogenetic analysis. This reveals a deep divergence between the two Asiatic linsang species (P. linsang, P. pardicolor), with an estimated divergence of ~12 million years (Ma). Although our sample size precludes any robust interpretation of the population structure of the banded linsang, we recover two distinct matrilines with an estimated tMRCA of ~1 Ma. Our results can be used as a basis for further investigation of the Asiatic linsangs, and further demonstrate the utility of ancestral capture for studying divergent taxa without close relatives.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 972 
KW  - Shotgun sequencing
KW  - Mitochondria
KW  - Phylogenetics
KW  - Phylogenetic analysis
KW  - Paleogenetics
KW  - Sequence alignment
KW  - Genomics
KW  - Museum collections
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-474441
SN  - 1866-8372
IS  - 972
ER  - 
TY  - JOUR
A1  - Paijmans, Johanna L. A.
A1  - Barlow, Axel
A1  - Henneberger, Kirstin
A1  - Fickel, Jörns
A1  - Hofreiter, Michael
A1  - Foerste, Daniel W. G.
T1  - Ancestral mitogenome capture of the Southeast Asian banded linsang
JF  - PLoS ONE
N2  - Utilising a reconstructed ancestral mitochondrial genome of a clade to design hybridisation capture baits can provide the opportunity for recovering mitochondrial sequences from all its descendent and even sister lineages. This approach is useful for taxa with no extant close relatives, as is often the case for rare or extinct species, and is a viable approach for the analysis of historical museum specimens. Asiatic linsangs (genus Prionodon) exemplify this situation, being rare Southeast Asian carnivores for which little molecular data is available. Using ancestral capture we recover partial mitochondrial genome sequences for seven banded linsangs (P. linsang) from historical specimens, representing the first intraspecific genetic dataset for this species. We additionally assemble a high quality mitogenome for the banded linsang using shotgun sequencing for time-calibrated phylogenetic analysis. This reveals a deep divergence between the two Asiatic linsang species (P. linsang, P. pardicolor), with an estimated divergence of ~12 million years (Ma). Although our sample size precludes any robust interpretation of the population structure of the banded linsang, we recover two distinct matrilines with an estimated tMRCA of ~1 Ma. Our results can be used as a basis for further investigation of the Asiatic linsangs, and further demonstrate the utility of ancestral capture for studying divergent taxa without close relatives.
KW  - Shotgun sequencing
KW  - Mitochondria
KW  - Phylogenetics
KW  - Phylogenetic analysis
KW  - Paleogenetics
KW  - Sequence alignment
KW  - Genomics
KW  - Museum collections
Y1  - 2019
U6  - https://doi.org/10.1371/journal.pone.0234385
SN  - 1932-6203
VL  - 15
IS  - 6
PB  - PLOS
CY  - San Francisco, California, US
ER  -