TY - JOUR A1 - Lisso, Janina A1 - Altmann, Thomas A1 - Müssig, Carsten T1 - The AtNFXL1 gene encodes a NF-X1 type zinc finger protein required for growth under salt stress JF - FEBS letters : the journal for rapid publication of short reports in molecular biosciences N2 - The human NF-X1 protein and homologous proteins in eukaryotes represent a class of transcription factors which are characterised. by NF-X1 type zinc finger motifs. The Arabidopsis genome encodes two NF-X1 homologs, which we termed AtNFXL1 and AtNFXL2. Growth and survival was impaired in atnfxl1 knock-out mutants and AtNFXL1-antisense plants under salt stress in comparison to wild-type plants. In contrast, 35S: :AtNFXL1 plants showed higher survival rates. The AtNFXL2 protein potentially plays an antagonistic role. The Arabidopsis NF-X1 type zinc finger proteins likely are part of regulatory mechanisms, which protect major processes such as photosynthesis. KW - Arabidopsis thaliana KW - NF-X1 KW - salt stress Y1 - 2006 U6 - https://doi.org/10.1016/j.febslet.2006.07.079 SN - 0014-5793 VL - 580 IS - 22 SP - 4851 EP - 4856 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Devkar, Vikas A1 - Thirumalaikumar, Venkatesh P. A1 - Xue, Gang-Ping A1 - Vallarino, Jose G. A1 - Tureckova, Veronika A1 - Strnad, Miroslav A1 - Fernie, Alisdair R. A1 - Hoefgen, Rainer A1 - Mueller-Roeber, Bernd A1 - Balazadeh, Salma T1 - Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress JF - New phytologist : international journal of plant science N2 - Salinity stress limits plant growth and has a major impact on agricultural productivity. Here, we identify NAC transcription factor SlTAF1 as a regulator of salt tolerance in cultivated tomato (Solanum lycopersicum). While overexpression of SlTAF1 improves salinity tolerance compared with wild-type, lowering SlTAF1 expression causes stronger salinity-induced damage. Under salt stress, shoots of SlTAF1 knockdown plants accumulate more toxic Na+ ions, while SlTAF1 overexpressors accumulate less ions, in accordance with an altered expression of the Na+ transporter genes SlHKT1;1 and SlHKT1;2. Furthermore, stomatal conductance and pore area are increased in SlTAF1 knockdown plants during salinity stress, but decreased in SlTAF1 overexpressors. We identified stress-related transcription factor, abscisic acid metabolism and defence-related genes as potential direct targets of SlTAF1, correlating it with reactive oxygen species scavenging capacity and changes in hormonal response. Salinity-induced changes in tricarboxylic acid cycle intermediates and amino acids are more pronounced in SlTAF1 knockdown than wild-type plants, but less so in SlTAF1 overexpressors. The osmoprotectant proline accumulates more in SlTAF1 overexpressors than knockdown plants. In summary, SlTAF1 controls the tomato’s response to salinity stress by combating both osmotic stress and ion toxicity, highlighting this gene as a promising candidate for the future breeding of stress-tolerant crops. KW - abscisic acid (ABA) KW - ion homeostasis KW - NAC KW - proline KW - salt stress KW - SlTAF1 KW - transcription factors Y1 - 2019 U6 - https://doi.org/10.1111/nph.16247 SN - 0028-646X SN - 1469-8137 VL - 225 IS - 4 SP - 1681 EP - 1698 PB - Wiley CY - Hoboken ER - TY - GEN A1 - des Aulnois, Maxime Georges A1 - Réveillon, Damien A1 - Robert, Elise A1 - Caruana, Amandine A1 - Briand, Enora A1 - Guljamow, Arthur A1 - Dittmann, Elke A1 - Amzil, Zouher A1 - Bormans, Myriam T1 - Salt shock responses of Microcystis revealed through physiological, transcript, and metabolomic analyses T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - The transfer of Microcystis aeruginosa from freshwater to estuaries has been described worldwide and salinity is reported as the main factor controlling the expansion of M. aeruginosa to coastal environments. Analyzing the expression levels of targeted genes and employing both targeted and non-targeted metabolomic approaches, this study investigated the effect of a sudden salt increase on the physiological and metabolic responses of two toxic M. aeruginosa strains separately isolated from fresh and brackish waters, respectively, PCC 7820 and 7806. Supported by differences in gene expressions and metabolic profiles, salt tolerance was found to be strain specific. An increase in salinity decreased the growth of M. aeruginosa with a lesser impact on the brackish strain. The production of intracellular microcystin variants in response to salt stress correlated well to the growth rate for both strains. Furthermore, the release of microcystins into the surrounding medium only occurred at the highest salinity treatment when cell lysis occurred. This study suggests that the physiological responses of M. aeruginosa involve the accumulation of common metabolites but that the intraspecific salt tolerance is based on the accumulation of specific metabolites. While one of these was determined to be sucrose, many others remain to be identified. Taken together, these results provide evidence that M. aeruginosa is relatively salt tolerant in the mesohaline zone and microcystin (MC) release only occurs when the capacity of the cells to deal with salt increase is exceeded. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1130 KW - Microcystis aeruginosa KW - microcystin KW - salt stress KW - metabolomic KW - transcript Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-472405 SN - 1866-8372 IS - 1130 ER - TY - JOUR A1 - des Aulnois, Maxime Georges A1 - Réveillon, Damien A1 - Robert, Elise A1 - Caruana, Amandine A1 - Briand, Enora A1 - Guljamow, Arthur A1 - Dittmann, Elke A1 - Amzil, Zouher A1 - Bormans, Myriam T1 - Salt shock responses of Microcystis revealed through physiological, transcript, and metabolomic analyses JF - Toxins N2 - The transfer of Microcystis aeruginosa from freshwater to estuaries has been described worldwide and salinity is reported as the main factor controlling the expansion of M. aeruginosa to coastal environments. Analyzing the expression levels of targeted genes and employing both targeted and non-targeted metabolomic approaches, this study investigated the effect of a sudden salt increase on the physiological and metabolic responses of two toxic M. aeruginosa strains separately isolated from fresh and brackish waters, respectively, PCC 7820 and 7806. Supported by differences in gene expressions and metabolic profiles, salt tolerance was found to be strain specific. An increase in salinity decreased the growth of M. aeruginosa with a lesser impact on the brackish strain. The production of intracellular microcystin variants in response to salt stress correlated well to the growth rate for both strains. Furthermore, the release of microcystins into the surrounding medium only occurred at the highest salinity treatment when cell lysis occurred. This study suggests that the physiological responses of M. aeruginosa involve the accumulation of common metabolites but that the intraspecific salt tolerance is based on the accumulation of specific metabolites. While one of these was determined to be sucrose, many others remain to be identified. Taken together, these results provide evidence that M. aeruginosa is relatively salt tolerant in the mesohaline zone and microcystin (MC) release only occurs when the capacity of the cells to deal with salt increase is exceeded. KW - Microcystis aeruginosa KW - microcystin KW - salt stress KW - metabolomic KW - transcript Y1 - 2020 U6 - https://doi.org/10.3390/toxins12030192 SN - 2072-6651 VL - 12 IS - 3 PB - MDPI CY - Basel ER - TY - JOUR A1 - Allu, Annapurna Devi A1 - Soja, Aleksandra Maria A1 - Wu, Anhui A1 - Szymanski, Jedrzej A1 - Balazadeh, Salma T1 - Salt stress and senescence: identification of cross-talk regulatory components JF - Journal of experimental botany N2 - Leaf senescence is an active process with a pivotal impact on plant productivity. It results from extensive signalling cross-talk coordinating environmental factors with intrinsic age-related mechanisms. Although many studies have shown that leaf senescence is affected by a range of external parameters, knowledge about the regulatory systems that govern the interplay between developmental programmes and environmental stress is still vague. Salinity is one of the most important environmental stresses that promote leaf senescence and thus affect crop yield. Improving salt tolerance by avoiding or delaying senescence under stress will therefore play an important role in maintaining high agricultural productivity. Experimental evidence suggests that hydrogen peroxide (H2O2) functions as a common signalling molecule in both developmental and salt-induced leaf senescence. In this study, microarray-based gene expression profiling on Arabidopsis thaliana plants subjected to long-term salinity stress to induce leaf senescence was performed, together with co-expression network analysis for H2O2-responsive genes that are mutually up-regulated by salt induced-and developmental leaf senescence. Promoter analysis of tightly co-expressed genes led to the identification of seven cis-regulatory motifs, three of which were known previously, namely CACGTGT and AAGTCAA, which are associated with reactive oxygen species (ROS)-responsive genes, and CCGCGT, described as a stress-responsive regulatory motif, while the others, namely ACGCGGT, AGCMGNC, GMCACGT, and TCSTYGACG were not characterized previously. These motifs are proposed to be novel elements involved in the H2O2-mediated control of gene expression during salinity stress-triggered and developmental senescence, acting through upstream transcription factors that bind to these sites. KW - Arabidopsis KW - hydrogen peroxide KW - longevity KW - reactive oxygen species KW - salt stress KW - senescence KW - signal cross-talk KW - transcription factor Y1 - 2014 U6 - https://doi.org/10.1093/jxb/eru173 SN - 0022-0957 SN - 1460-2431 VL - 65 IS - 14 SP - 3993 EP - 4008 PB - Oxford Univ. Press CY - Oxford ER -