TY  - JOUR
A1  - Hassan, Rabeay Y. A.
A1  - Wollenberger, Ursula
T1  - Mediated bioelectrochemical system for biosensing the cell viability of Staphylococcus aureus
JF  - Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis
N2  - Staphylococcus aureus is one of the most dangerous human pathogens and is the cause of numerous illnesses ranging from moderate skin infections to life-threatening diseases. Despite advances made in identifying microorganisms, rapid detection methods for the viability of bacteria are still missing. Here, we report a rapid electrochemical assay for cell viability combining the use of double redox mediators and multiwall carbon nanotubes-screen printed electrodes (MWCNTs-SPE), ferricyanide (FCN) and 2,6-dichlorophenolindophenol (DCIP), which served as electron shuttle to enable the bacterial-electrode communications. The current originating from the metabolically active cells was recorded for probing the activity of the intracellular redox centers. Blocking of the respiratory chain pathways with electron transfer inhibitors demonstrated the involvement of the electron transport chain in the reaction. A good correlation between the number of the metabolically active cells and the current was obtained. The proposed assay has been exploited for monitoring cell proliferation of S. aureus during the growth. The sensitivity of the detection method reached 0.1 OD600. Therefore, the technique described is promising for estimating the cell number, measuring the cell viability, and probing intracellular redox center(s).
KW  - Microbial electrochemistry
KW  - Pathogenic detection
KW  - Probing living Staphylococcus aureus
KW  - CNTs-based screen printed electrodes
Y1  - 2016
U6  - https://doi.org/10.1007/s00216-015-9134-z
SN  - 1618-2642
SN  - 1618-2650
VL  - 408
SP  - 579
EP  - 587
PB  - Springer
CY  - Heidelberg
ER  -