TY  - JOUR
A1  - Hentrich, Doreen
A1  - Tauer, Klaus
A1  - Espanol, Montserrat
A1  - Ginebra, Maria-Pau
A1  - Taubert, Andreas
T1  - EDTA and NTA effectively tune the mineralization of calcium phosphate from bulk aqueous solution
JF  - Biomimetics
N2  - This study describes the effects of nitrilotriacetic acid (NTA) and ethylenediaminotetraacetic acid (EDTA) on themineralization of calciumphosphate from bulk aqueous solution. Mineralization was performed between pH 6 and 9 and with NTA or EDTA concentrations of 0, 5, 10, and 15 mM. X-ray diffraction and infrared spectroscopy show that at low pH, mainly brushite precipitates and at higher pH, mostly hydroxyapatite forms. Both additives alter the morphology of the precipitates. Without additive, brushite precipitates as large plates. With NTA, the morphology changes to an unusual rod-like shape. With EDTA, the edges of the particles are rounded and disk-like particles form. Conductivity and pH measurements suggest that the final products form through several intermediate steps.
KW  - biomineralization
KW  - biomimetic mineralization
KW  - calcium phosphate
KW  - NTA
KW  - EDTA
KW  - precipitation
KW  - brushite
KW  - hydroxyapatite
Y1  - 2017
U6  - https://doi.org/10.3390/biomimetics2040024
SN  - 2313-7673
VL  - 2
IS  - 4
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Hamed Misbah, Mohamed
A1  - Santos, Mercedes
A1  - Quintanilla, Luis
A1  - Günter, Christina
A1  - Alonso, Matilde
A1  - Taubert, Andreas
A1  - Carlos Rodriguez-Cabello, Jose
T1  - Recombinant DNA technology and click chemistry: a powerful combination for generating a hybrid elastin-like-statherin hydrogel to control calcium phosphate mineralization
JF  - Beilstein journal of nanotechnology
N2  - Understanding the mechanisms responsible for generating different phases and morphologies of calcium phosphate by elastin-like recombinamers is supreme for bioengineering of advanced multifunctional materials. The generation of such multifunctional hybrid materials depends on the properties of their counterparts and the way in which they are assembled. The success of this assembly depends on the different approaches used, such as recombinant DNA technology and click chemistry. In the present work, an elastin-like recombinamer bearing lysine amino acids distributed along the recombinamer chain has been cross-linked via Huisgen [2 + 3] cycloaddition. The recombinamer contains the SN(A)15 peptide domains inspired by salivary statherin, a peptide epitope known to specifically bind to and nucleate calcium phosphate. The benefit of using click chemistry is that the hybrid elastin-like-statherin recombinamers cross-link without losing their fibrillar structure. Mineralization of the resulting hybrid elastin-like-statherin recombinamer hydrogels with calcium phosphate is described. Thus, two different hydroxyapatite morphologies (cauliflower- and plate-like) have been formed. Overall, this study shows that crosslinking elastin-like recombinamers leads to interesting matrix materials for the generation of calcium phosphate composites with potential applications as biomaterials.
KW  - calcium phosphate
KW  - elastin-like recombinamers
KW  - hydroxyapatite
KW  - mineralization
KW  - SN(A)15
Y1  - 2017
U6  - https://doi.org/10.3762/bjnano.8.80
SN  - 2190-4286
VL  - 8
SP  - 772
EP  - 783
PB  - Beilstein-Institut zur Förderung der Chemischen Wissenschaften
CY  - Frankfurt, Main
ER  -