TY  - JOUR
A1  - Eerqing, Narima
A1  - Subramanian, Sivaraman
A1  - Rubio Jimenez, Jesus
A1  - Lutz, Tobias
A1  - Wu, Hsin-Yu
A1  - Anders, Janet
A1  - Soeller, Christian
A1  - Vollmer, Frank
T1  - Comparing transient oligonucleotide hybridization kinetics using DNA-PAINT and optoplasmonic single-molecule sensing on gold nanorods
JF  - ACS photonics / American Chemical Society
N2  - We report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing. As the test system, oligonucleotides with and without fluorescent labels are transiently hybridized to complementary "docking" strands attached to gold nanorods. Comparing the measured single-molecule kinetics helps to examine the influence of the fluorescent labels as well as factors arising from different sensing geometries. Our results demonstrate that DNA dissociation is not significantly altered by the fluorescent labels and that DNA association is affected by geometric factors in the two techniques. These findings open the door to exploiting plasmonic sensing and fluorescence nanoscopy in a complementary fashion, which will aid in building more powerful sensors and uncovering the intricate effects that influence the behavior of single molecules.
KW  - single-molecule
KW  - plasmonics
KW  - whispering gallery modes
KW  - optoplasmonic
KW  - DNA-PAINT
KW  - fluorescence
KW  - localization microscopy
Y1  - 2021
U6  - https://doi.org/10.1021/acsphotonics.1c01179
SN  - 2330-4022
VL  - 8
IS  - 10
SP  - 2882
EP  - 2888
PB  - American Chemical Society
CY  - Washington
ER  -