TY  - JOUR
A1  - Andrés-Delgado, Laura
A1  - Ernst, Alexander
A1  - Galardi-Castilla, María
A1  - Bazaga, David
A1  - Peralta, Marina
A1  - Münch, Juliane
A1  - Gonzalez-Rosa, Juan M.
A1  - Marques, Inês
A1  - Tessadori, Federico
A1  - de la Pompa, José Luis
A1  - Vermot, Julien
A1  - Mercader, Nadia
T1  - Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells
JF  - Development : Company of Biologists
N2  - The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.
KW  - Actomyosin
KW  - Bmp
KW  - Cell extrusion
KW  - Proepicardium
KW  - Zebrafish
KW  - Heart development
Y1  - 2019
U6  - https://doi.org/10.1242/dev.174961
SN  - 0950-1991
SN  - 1477-9129
VL  - 146
IS  - 13
PB  - The Company of Biologists Ltd
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Lombardo, Verónica A.
A1  - Heise, Melina
A1  - Moghtadaei, Motahareh
A1  - Bornhorst, Dorothee
A1  - Männer, Jörg
A1  - Abdelilah-Seyfried, Salim
T1  - Morphogenetic control of zebrafish cardiac looping by Bmp signaling
JF  - Development : Company of Biologists
N2  - Cardiac looping is an essential and highly conserved morphogenetic process that places the different regions of the developing vertebrate heart tube into proximity of their final topographical positions. High-resolution 4D live imaging of mosaically labelled cardiomyocytes reveals distinct cardiomyocyte behaviors that contribute to the deformation of the entire heart tube. Cardiomyocytes acquire a conical cell shape, which is most pronounced at the superior wall of the atrioventricular canal and contributes to S-shaped bending. Torsional deformation close to the outflow tract contributes to a torque-like winding of the entire heart tube between its two poles. Anisotropic growth of cardiomyocytes based on their positions reinforces S-shaping of the heart. During cardiac looping, bone morphogenetic protein pathway signaling is strongest at the future superior wall of the atrioventricular canal. Upon pharmacological or genetic inhibition of bone morphogenetic protein signaling, myocardial cells at the superior wall of the atrioventricular canal maintain cuboidal cell shapes and S-shaped bending is impaired. This description of cellular rearrangements and cardiac looping regulation may also be relevant for understanding the etiology of human congenital heart defects.
KW  - BMP
KW  - Wnt
KW  - Cardiac looping
KW  - Hemodynamics
KW  - Zebrafish
Y1  - 2019
U6  - https://doi.org/10.1242/dev.180091
SN  - 0950-1991
SN  - 1477-9129
VL  - 146
IS  - 22
PB  - The Company of Biologists Ltd
CY  - Cambridge
ER  -