TY  - JOUR
A1  - Baxa, Ulrich
A1  - Cooper, Alan
A1  - Weintraub, N.
A1  - Pfeil, Wolfgang
A1  - Seckler, Robert
T1  - Enthalpic barriers to the hydrophobic binding of oligosaccharides to phage P22 tailspike protein
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Burova, Tatjana V.
A1  - Beckert, Vita
A1  - Uhlmann, Heike
A1  - Ristau, Otto
A1  - Bernhardt, Rita
A1  - Pfeil, Wolfgang
T1  - Conformational stability of adrenodoxin mutant proteins
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Burova, Tatjana V.
A1  - Bernhardt, Rita
A1  - Pfeil, Wolfgang
T1  - Conformational stability of holo and apo adrenodoxin : a scanning calorimetric study
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Damaschun, Gregor
A1  - Damaschun, Hilde
A1  - Gast, Klaus
A1  - Misselwitz, Rolf
A1  - Müller, Jürgen J.
A1  - Pfeil, Wolfgang
A1  - Zirwer, Dietrich
T1  - Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Dudich, Igor V.
A1  - Zav`ylov, Vladimir
A1  - Pfeil, Wolfgang
A1  - Gaestel, Matthias
A1  - Zav`yalova, Galina A.
A1  - Denesyuk, Alexandr I.
A1  - Korpela, Timo
T1  - Dimer structure as a minimum cooperative subunit of small heat shock-proteins
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Freiberg, Alexander
A1  - Machner, M. P.
A1  - Pfeil, Wolfgang
A1  - Schubert, W. D.
A1  - Heinz, Dirk W.
A1  - Seckler, Robert
T1  - Folding and stability of the leucine-rich repeat domain of internalin B from Listeria monocytogenes
N2  - Internalin B (InlB), a surface protein of the human pathogen Listeria monocytogenes, promotes invasion into various host cell types by inducing phagocytosis of the entire bacterium. The N-terminal half of InlB (residues 36-321, InlB(321)), which is sufficient for this process, contains a central leucine-rich repeat (LRR) domain that is flanked by a small a-helical cap 2 and an immunoglobulin (Ig)-like domain. Here we investigated the variant lacking the Ig-like domain (lnlB(248)). The circular dichroism spectra of both protein variants in the far ultraviolet region are very similar, with a characteristic minimum found at similar to200 nm, possibly resulting from the high 3(10)-helical content in the LRR domain. Upon addition of chemical denaturants, both variants unfold in single transitions with unusually high cooperativity that are fully reversible and best described by two-state equilibria. The free energies of GdmCl-induced unfolding determined from transitions at 20degreesC are 9.9(+/- 0.8)kcal/mol for InlB(321) and 5.4(+/- 0.4) kcal/mol for InlB(248). InlB(321) is also more stable against thermal denaturation, as observed by scanning calorimetry. This suggests, that the Ig-like domain, which presumably does not directly interact with the host cell receptor during bacterial invasion, plays a critical role for the in vivo stability of InlB. (C) 2004 Elsevier Ltd. All rights reserved
Y1  - 2004
SN  - 0022-2836
ER  - 
TY  - JOUR
A1  - Gast, Klaus
A1  - Damaschun, Gregor
A1  - Desmadril, Michel
A1  - Minard, Philippe
A1  - Müller-Frohne, Marlies
A1  - Pfeil, Wolfgang
A1  - Zirwer, Dietrich
T1  - Cold denaturation of yeast phosphoglycerate kinase : which domain is more stable?
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Gesierich, Ulrike
A1  - Pfeil, Wolfgang
T1  - The conformational stability of - crystallin is rather low : calorimetric results
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Goder, V.
A1  - Beckert, Vita
A1  - Pfeil, Wolfgang
A1  - Bernhardt, Rita
T1  - Impact of the presequence of a mitochondrium targeted precursor, preadrenodoxin, on folding, catalytic activity and stability of the protein in vitro
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Jung, Christiane
A1  - Pfeil, Wolfgang
A1  - Köpke, Karla
A1  - Schulze, Heike
A1  - Ristau, Otto
T1  - Conformational states and substates of cytochrome P450cam - insight in protein dynamics and folding
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Koroljova, Olga V.
A1  - Stepanova, Elena V.
A1  - Binukov, Vladimir I.
A1  - Timofeev, Vladimir P.
A1  - Pfeil, Wolfgang
T1  - Temperature-induced changes in copper centers and protein conformation of two fungal laccases from Coriolus hirsutus and Coriolus zonatus
Y1  - 2001
ER  - 
TY  - JOUR
A1  - Krylov, Andrey V.
A1  - Pfeil, Wolfgang
A1  - Lisdat, Fred
T1  - Denaturation and renaturation of cytochrome c immobilized on gold electrodes in DMSO-containing buffers
N2  - Cytochrome c (cyt c) was immobilized on surface-modified gold electrodes using a self-assembling approach. The resulting cyt c electrode was studied using cyclic voltammetry. Compared to pure phosphate buffer, cyt c electrodes exhibited in DMSO-containing solutions lower oxidation and reduction peak currents, which originated from a decrease in the addressable electro-active amount of the surface-immobilized protein. This is associated with the process of protein denaturation. The denaturation kinetics can be described by a sum of two processes with time constants differing by more than one order of magnitude. The subsequent change of the aqueous/organic medium back to a pure aqueous buffer resulted in a shift of the formal potential to its initial value and a partial recovery of the peak current. This can be attributed to the renaturation of the cyt c. The extent of renaturation depended on the organic solvent/water ratio of the mixture used. The kinetics of protein renaturation were similar to those of the denaturation process. (C) 2004 Elsevier B.V. All rights reserved
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Lasch, Jürgen
A1  - Zellmer, Sebastian
A1  - Pfeil, Wolfgang
A1  - Schubert, Rolf
T1  - Interaction of liposomes with the human skin lipid barrier : hSCLLs as model system - DSC of intact stratum corneum and in situ CLSM of human skin
Y1  - 1995
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
T1  - The influence of glycosylation on the thermal stability of ribonuclease
Y1  - 2002
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
T1  - Thermodynamics of apocytochrome b5 unfolding
Y1  - 1993
ER  - 
TY  - BOOK
A1  - Pfeil, Wolfgang
T1  - Protein stability and folding : a collection of thermodynamic data
Y1  - 1998
SN  - 3-540-63717-6
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
T1  - Is the molten globule a third thermodynamic state of protein? : the exemplare of à-Lactalbumin
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
T1  - Partly folded proteins (Minireview)
Y1  - 1998
SN  - 0320-9725
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
T1  - Thermodynamische Untersuchungen an Proteinen
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
T1  - Native-like intermediates in protein folding
Y1  - 1994
ER  - 
TY  - BOOK
A1  - Pfeil, Wolfgang
T1  - Protein stability and folding : a collecton of thermodynamic data ; Supplement 1
Y1  - 2001
SN  - 3-540-42168-8
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
A1  - Burova, Tatjana V.
A1  - Beckert, Vita
A1  - Uhlmann, Heike
A1  - Ristau, Otto
A1  - Bernhardt, Rita
T1  - Conformational stability of adrenodoxin mutant proteins
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
A1  - Gesierich, Ulrike
A1  - Kleemann, G. R.
A1  - Sterner, Reinhard
T1  - Ferredoxin from the hyperthermophiele thermotoga maritima os stable beyond the boiling point of watter
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
A1  - Gesierich, Ulrike
A1  - Sterner, Reinhard
T1  - The 4Fe-4S ferredoxin from thermotoga maritima is extremely thermostable
Y1  - 1996
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
A1  - Nölting, Bengt
A1  - Jung, Christiane
T1  - Apocytochrome P450cam is a native protein with some intermediate-like properties
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Pfeil, Wolfgang
A1  - Nölting, Bengt
A1  - Jung, Christiane
T1  - Thermodynmic properties of apocytochrome P450cam
Y1  - 1993
ER  - 
TY  - JOUR
A1  - Rishi, Vikas
A1  - Anjum, Farah
A1  - Ahmad, Faizan
A1  - Pfeil, Wolfgang
T1  - Role of non-compatible osmolytes in the stabilization of proteins during heat stress
Y1  - 1998
ER  - 
TY  - JOUR
A1  - Roske, Y.
A1  - Sunna, A.
A1  - Pfeil, Wolfgang
A1  - Heinemann, Udo
T1  - High-resolution crystal structures of Caldiceflulosiruptor strain Rt8B.4 carbohydrate-binding module CBM27-1 and its complex with mannohexaose
N2  - Carbohydrate-binding modules (CBMs) are the most common non-catalytic modules associated with enzymes active in plant cell-wall hydrolysis. Despite the large number of putative CBMs being identified by amino acid sequence alignments, only few representatives have been experimentally shown to have a carbohydrate-binding function. Caldicellulosiruptor strain Rt8B.4 Man26 is a thermostable modular glycoside hydrolase beta-mannanase which contains two non-catalytic modules in tandem at its N terminus. These modules were recently shown to function primarily as beta- mannan-binding modules and have accordingly been classified as members of a novel family of CBMs, family 27. The N- terminal CBM27 (CsCBM27-1) of Man26 from Caldicellulosiruptor Rt8B.4 displays high-binding affinity towards mannohexaose with a K-a of 1 x 10(7) M-1. Accordingly, the high-resolution crystal structures of CsCBM27-1 native and its mannohexaose complex were solved at 1.55 Angstrom and 1.06 Angstrom resolution, respectively. In the crystal, CsCBM27-1 shows the typical beta-sandwich jellyroll fold observed in other CBMs with a single metal ion bound, which was identified as calcium. The crystal structures reveal that the overall fold of CsCBM27-1 remains virtually unchanged upon sugar binding and that binding is mediated by three solvent-exposed tryptophan residues and few direct hydrogen bonds. Based on binding affinity and thermal unfolding experiments this structural calcium is shown to play a role in the thermal stability of CsCBM27-1 at high temperatures. The higher binding affinity of CsCBM27-1 to mannooligosaccharides when compared to other members of CBM family 27 might be explained by the different orientation of the residues forming the "aromatic platform" and by differences in the length of loops. Finally, evidence is presented, on the basis of fold similarities and the retention of the position of conserved motifs and a calcium ion, for the consolidation of related CBM families into a superfamily of CBMs. (C) 2004 Elsevier Ltd. All rights reserved
Y1  - 2004
SN  - 0022-2836
ER  - 
TY  - JOUR
A1  - Schubert, Dieter
A1  - Schluckebier, Gerd
A1  - Backmann, Jan
A1  - Granzien, Joachim
A1  - Kisker, Caroline
A1  - Choe, Hui-Woog
A1  - Hahn, Ulrich
A1  - Pfeil, Wolfgang
A1  - Saenger, Wolfram
T1  - X-ray crystallographic and calorimetric studies of the effects of the mutation Trp59Tyr in ribonuclease T1
Y1  - 1994
ER  - 
TY  - JOUR
A1  - Schwarz, D.
A1  - Kissele, P.
A1  - Pfeil, Wolfgang
A1  - Pisch, S.
A1  - Bornscheuer, U.
A1  - Schmid, Rolf D.
T1  - Evidence that nonbilayer phase probensity of the membrane is important for thr side chain cleavage of cytochrome P450SCC (CYP11A1)
Y1  - 1997
ER  - 
TY  - JOUR
A1  - Zellmer, Sebastian
A1  - Pfeil, Wolfgang
A1  - Lasch, Jürgen
T1  - Interaction of phosphatidylcholine liposomes with the human stratum corneum
Y1  - 1995
ER  -