TY  - JOUR
A1  - Uhlig, Katja
A1  - Wischerhoff, Erik
A1  - Lutz, Jean-Francois
A1  - Laschewsky, André
A1  - Jäger, Magnus S.
A1  - Lankenau, Andreas
A1  - Duschl, Claus
T1  - Monitoring cell detachment on PEG-based thermoresponsive surfaces using TIRF microscopy
N2  - Recently, we introduced a thermoresponsive copolymer that consists of oligo(ethylene glycol) methacrylate (OEGMA) and 2-(2- methoxyethoxy) ethyl methacrylate (MEO(2)MA). The polymer exhibited an LCST at 35 degrees C in PBS buffer and was anchored onto gold substrates using disulfide polymerisation initiators. It allows the noninvasive detachment of adherent cells from their substrate. As the mechanisms that determine the interaction of cells with such polymers are not well understood, we employed Total Internal Reflection Fluorescence (TIRF) microscopy in order to monitor the detachment process of cells of two different types. We identified contact area and average cell-substrate distance as crucial parameters for the evaluation of the detachment process. The sensitivity of TIRF microscopy allowed us to correlate the specific adhesion pattern of MCF-7 breast cancer cells with the morphology of cell deposits that may serve as fingerprints for a nondestructive characterisation of live cells.
Y1  - 2010
UR  - http://www.rsc.org/Publishing/Journals/sm/index.asp
U6  - https://doi.org/10.1039/C0sm00010h
SN  - 1744-683X
ER  - 
TY  - JOUR
A1  - Wischerhoff, Erik
A1  - Badi, Nezha
A1  - Lutz, Jean-Francois
A1  - Laschewsky, André
T1  - Smart bioactive surfaces
N2  - The purpose of this highlight is to define the emerging field of bioactive surfaces. In recent years, various types of synthetic materials capable of "communicating'' with biological objects such as nucleic acids, proteins, polysaccharides, viruses, bacteria or living cells have been described in the literature. This novel area of research certainly goes beyond the traditional field of smart materials and includes different types of sophisticated interactions with biological entities, such as reversible adhesion, conformational control, biologically-triggered release and selective permeation. These novel materials may be 2D planar surfaces as well as colloidal objects or 3D scaffolds. Overall, they show great promise for numerous applications in biosciences and biotechnology. For instance, practical applications of bioactive surfaces in the fields of bioseparation, cell engineering, biochips and stem-cell differentiation are briefly discussed herein.
Y1  - 2010
UR  - http://www.rsc.org/Publishing/Journals/sm/index.asp
U6  - https://doi.org/10.1039/B913594d
SN  - 1744-683X
ER  -