TY  - JOUR
A1  - Ast, Sandra
A1  - Müller, Holger
A1  - Flehr, Roman
A1  - Klamroth, Tillmann
A1  - Walz, Bernd
A1  - Holdt, Hans-Jürgen
T1  - High Na+ and K+-induced fluorescence enhancement of a pi-conjugated phenylaza-18-crown-6-triazol-substituted coumarin fluoroionophore
JF  - Chemical communications
N2  - The new pi-conjugated 1,2,3-triazol-1,4-diyl fluoroionophore 1 generated via Cu(I) catalyzed [3 + 2] cycloaddition shows high fluorescence enhancement factors (FEF) in the presence of Na+ (FEF = 58) and K+ (FEF = 27) in MeCN and high selectivity towards K+ under simulated physiological conditions (160 mM K+ or Na+, respectively) with a FEF of 2.5 for K+.
Y1  - 2011
U6  - https://doi.org/10.1039/c0cc04370b
SN  - 1359-7345
VL  - 47
IS  - 16
SP  - 4685
EP  - 4687
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Kienzler, Andrea
A1  - Flehr, Roman
A1  - Kramer, Rolf A.
A1  - Gehne, Soeren
A1  - Kumke, Michael Uwe
A1  - Bannwarth, Willi
T1  - Novel Three-Color FRET Tool Box for Advanced Protein and DNA Analysis
JF  - Bioconjugate chemistry
N2  - We report on a new three-color FRET system which we were able to verify in peptides as well as in synthetic DNA. All three chromophores could be introduced by a building block approach avoiding postsynthetic labeling. Additional features are robustness, matching spectroscopic properties, high-energy transfer, and sensitivity. The system was investigated in detail on a set of peptides as well as an array of tailored oligonucleotides. The detailed analysis of the experimental data and comparison with theoretical considerations were in excellent agreement. It is shown that in the case of polypeptides specific interaction with the fluorescence probes has to be considered. In contrast with DNA, the fluorescence probes did not show any indications of such interactions. The novel three-color FRET toolbox revealed the potential for applications studying fundamental processes of three interacting molecules in life science applications.
Y1  - 2011
U6  - https://doi.org/10.1021/bc2002659
SN  - 1043-1802
VL  - 22
IS  - 9
SP  - 1852
EP  - 1863
PB  - American Chemical Society
CY  - Washington
ER  -