TY  - JOUR
A1  - Bader, Denise
A1  - Klier, Dennis Tobias
A1  - Hettrich, C.
A1  - Bier, Frank Fabian
A1  - Wessig, Pablo
T1  - Detecting carbohydrate-lectin interactions using a fluorescent probe based on DBD dyes
JF  - Analytical methods : advancing methods and applications
N2  - Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein - a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.
Y1  - 2016
U6  - https://doi.org/10.1039/c5ay02991k
SN  - 1759-9660
SN  - 1759-9679
VL  - 8
SP  - 1235
EP  - 1238
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Wessig, Pablo
A1  - Bader, Denise
A1  - Klier, Dennis Tobias
A1  - Hettrich, Cornelia
A1  - Bier, Frank Fabian
T1  - Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes
N2  - Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein – a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 314 
KW  - conformational-changes
KW  - green-i
KW  - protein
KW  - binding
KW  - assay
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-394382
SP  - 1235
EP  - 1238
ER  -