TY - JOUR A1 - Puppe, Daniel A1 - Höhn, Axel A1 - Kaczorek, Danuta A1 - Wanner, Manfred A1 - Sommer, Michael T1 - As time goes by-Spatiotemporal changes of biogenic Si pools in initial soils of an artificial catchment in NE Germany JF - Applied soil ecology : a section of agriculture, ecosystems & environment KW - Biogenic silica KW - Diatom frustule KW - Testate amoeba shell KW - Sponge spicule KW - Initial ecosystem Y1 - 2016 U6 - https://doi.org/10.1016/j.apsoil.2016.01.020 SN - 0929-1393 SN - 1873-0272 VL - 105 SP - 9 EP - 16 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wanner, Manfred A1 - Seidl-Lampa, Barbara A1 - Höhn, Axel A1 - Puppe, Daniel A1 - Meisterfeld, Ralf A1 - Sommer, Michael T1 - Culture growth of testate amoebae under different silicon concentrations JF - European journal of protistology N2 - Testate amoebae with self-secreted siliceous shell platelets ("idiosomes") play an important role in terrestrial silicon (Si) cycles. In this context, Si-dependent culture growth dynamics of idiosomic testate amoebae are of interest. Clonal cultures of idiosomic testate amoebae were analyzed under three different Si concentrations: low (50 mu mol L-1), moderate/site-specific (150 mu mol L-1) and high Si supply (500 mu mol L-1). Food (Saccharomyces cerevisiae) was provided in surplus. (i) Shell size of four different clones of idiosomic testate amoebae either decreased (Trinema galeata, Euglypha filifera cf.), increased (E. rotunda cf.), or did not change (E. rotunda) under the lowest Si concentration (50 mu mol Si L-1). (ii) Culture growth of idiosomic Euglypha rotunda was dependent on Si concentration. The more Si available in the culture medium, the earlier the entry into exponential growth phase. (iii) Culture growth of idiosomic Euglypha rotunda was dependent on origin of inoculum. Amoebae previously cultured under a moderate Si concentration revealed highest sustainability in consecutive cultures. Amoebae derived from cultures with high Si concentrations showed rapid culture growth which finished early in consecutive cultures. (iv) Si (diluted in the culture medium) was absorbed by amoebae and fixed in the amoeba shells resulting in decreased Si concentrations. (C) 2016 Elsevier GmbH. All rights reserved. KW - Amoebal silicon KW - Sommer et al. 2006 KW - Biosilicification KW - Consumption KW - Culture growth dynamics KW - Euglyphida KW - Terrestrial Si cycle Y1 - 2016 U6 - https://doi.org/10.1016/j.ejop.2016.08.008 SN - 0932-4739 SN - 1618-0429 VL - 56 SP - 171 EP - 179 PB - Royal Society of Chemistry CY - Jena ER -