TY - JOUR A1 - Petersen, Hans-Georg T1 - Economic Aspects of Agricultural Areas Management and Land/Water Ecotones Conservation Y1 - 2001 ER - TY - JOUR A1 - Gessinger, Joachim T1 - Deutsche reden Deutsch Y1 - 2001 SN - 3-631-37009- 1 ER - TY - JOUR A1 - Gessinger, Joachim T1 - Von der Sinnlichkeit im Erkennen Y1 - 2001 SN - 3-631-37639-1 ER - TY - BOOK A1 - Edelstein, Wolfgang A1 - Grözinger, Karl Erich A1 - Gruehn, Sabine A1 - Hillerich, Imma A1 - Kirsch, Bärbel A1 - Leschinsky, Achim A1 - Lott, Jürgen A1 - Oser, Fritz T1 - Lebensgestaltung-Ethik-Religionskunde : zur Grundlegung eines neuen Schulfaches, Analysen und Empfehlungen Y1 - 2001 SN - 3-407-32001-9 PB - Beltz CY - Weinheim ER - TY - JOUR A1 - Anishchenko, Vadim S. A1 - Vadivasova, T. E. A1 - Kopeikin, A. S. A1 - Kurths, Jürgen A1 - Strelkova, G. I. T1 - Effect of noise on the relaxation to an invariant probability measure of nonhyperbolic chaotic attractors Y1 - 2001 ER - TY - JOUR A1 - Grözinger, Elvira T1 - Brandt, K. (Hrsg.), Vor der Auslöschung : Fotografien gefunden in Auschwitz; München, Kehayoff, 2001 BT - Vor der Auslöschung : Fotografien gefunden in Auschwitz Y1 - 2001 ER - TY - JOUR A1 - Giest, Hartmut T1 - Instruction and learning in elementary school Y1 - 2001 SN - 87-7288-841-5 ER - TY - BOOK ED - Petersen, Hans-Georg T1 - DIW-Wochenbericht Y1 - 2001 PB - Deutsches Institut für Wirtschaftsforschung CY - Berlin ER - TY - THES A1 - Kossow, Dirk T1 - Die kinematische Entwicklung des invertierten, intrakontinentalen Nordostdeutschen Beckens T2 - Scientific Technical Reports Y1 - 2001 UR - http://www.gfz-potsdam.de/bib/pub/str0204/0204.pdf UR - https://nbn-resolving.org/urn:nbn:de:kobv:b103-020113 VL - STR 02/04 ER - TY - JOUR A1 - Lindner, A. A1 - Freese, H. A1 - Quehl, G. A1 - Reiß, D. A1 - Schiller, K. T1 - Jaynes-Cummings-model with damping at resonance Y1 - 2001 SN - 1434-6060 ER - TY - BOOK ED - Duvinage, Brigitte T1 - Alltagsstoffe, Stoffe, Reaktionen : von " Stoffe um uns" bis zu "Reaktion gesucht" ; Klassen 7 bis 10 T3 - Z.E.U.S.-Materialien : Chemie Y1 - 2001 SN - 3-7614-3367-9 VL - 1 PB - Aulis CY - Köln ER - TY - JOUR A1 - Grözinger, Elvira T1 - Kaddisch für das Röslein auf der Heide : über die Tragik der jüdisch-deutschen Volksliedseligkeit Y1 - 2001 ER - TY - JOUR A1 - Daly, J. S. A1 - Balagansky, V. V. A1 - Timmerman, Martin Jan A1 - Whitehouse, M. J. A1 - de Jong, K. A1 - Guise, P. A1 - Bogdanova, S. A1 - Gorbatschev, R. A1 - Bridgwater, D. T1 - Ion microprobe U-Pb zircon geochronology and isotopic evidence for a trans-crustal suture in the Lapland-Kola Orogen, northern Fennoscandian Shield Y1 - 2001 ER - TY - JOUR A1 - Bridgwater, D. A1 - Scott, D. J. A1 - Balagansky, V. V. A1 - Timmerman, Martin Jan A1 - Marker, Michael A1 - Bushmin, S. S. A1 - Alexeyev, N. L. A1 - Daly, J. S. T1 - Age and provenance of early Precambrian metasedimentary rocks in the Lapland-Kola Belt, Russia : evidence from Pb and Nd isotopic data Y1 - 2001 ER - TY - JOUR A1 - Pecher, Gabriele A1 - Spahn, Gunter A1 - Schirrmann, Thomas A1 - Kulbe, Hagen A1 - Ziegner, Maja A1 - Schenk, Jörg A. A1 - Sandig, Volker T1 - Mucin gene (MUC1) transfer into human dendritic cells by cationic liposomes and recombinant adenovirus N2 - BACKGROUND: Dendritic cells (DC) as antigen presenting cells play an important role in immunotherapy of cancer. Mucin, encoded by the gene MUC1, is a human tumor antigen expressed in breast, pancreatic and ovarian cancers. Therefore, MUC1-transfected DC would be an attractive tool in constructing cancer vaccines. MATERIALS AND METHODS: Using two different cationic liposome preparations and, for comparison, a recombinant adenovirus expressing mucin, we tested the efficiency of mucin gene transfer into DC by flow cytometry. We investigated if these transfected DC were able to specifically stimulate autologous peripheral blood lymphocytes (PBL) from healthy donors. RESULTS: Flow cytometry revealed that 5-20% of DC transfected with liposomes Lipofectin and 20-40% of DC transduced with adenovirus expressed the relevant mucin epitopes. The expression of mucin on DC was similar to the expression of mucin found on carcinoma cells. After antigen uptake, DC specifically stimulated autologous PBL. CONCLUSION: We have shown that cationic liposomal gene transfer into human DC was feasible. We could obtain antigen specific stimulation of PBL at a similar rate as with adenoviral MUC1-transduced DC. Y1 - 2001 SN - 0250-7005 ER - TY - JOUR A1 - Pecher, Gabriele A1 - Harnack, U. A1 - Gunther, M. A1 - Hummel, M. A1 - Fichtner, I. A1 - Schenk, Jörg A. T1 - Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice. N2 - Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the alpha/beta-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy. Y1 - 2001 UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-45S4JD7- VM&_coverDate=05%2F18%2F2001&_alid=268965202&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6713&_sort=d&view=c&_acct=C000053886&_ v ER - TY - JOUR A1 - Bergholz, André A1 - Heymann, Stephan A1 - Schenk, Jörg A. A1 - Freytag, Johann Christoph T1 - Biological sequences integrated: a relational database approach N2 - Over the last decade the modeling and the storage of biological data has been a topic of wide interest for scientists dealing with biological and biomedical research. Currently most data is still stored in text files which leads to data redundancies and file chaos. In this paper we show how to use relational modeling techniques and relational database technology for modeling and storing biological sequence data, i.e. for data maintained in collections like EMBL or SWISS-PROT to better serve the needs for these application domains. For this reason we propose a two step approach. First, we model the structure (and therefore the meaning of the) data using an Entity-Relationship approach. The ER model leads to a clean design of a relational database schema for storing and retrieving the DNA and protein data extracted from various sources. Our approach provides the clean basis for building complex biological applications that are more amenable to changes and software ports than their file-base counterparts. Y1 - 2001 UR - http://www.springerlink.com/app/home/ contribution.asp?wasp=161c4c19086a4dceac9312b46e5f2348&referrer=parent&backto=issue,1,5;journal,14,29;linkingpublicationr esults,1:102835,1 ER - TY - JOUR A1 - Kroener, Bernhard R. T1 - Von der Schwarzen Magie zur "ultima ratio regum" Y1 - 2001 SN - 3-87909-751-8 ER - TY - JOUR A1 - Balagansky, V. V. A1 - Timmerman, Martin Jan A1 - Kozlova, N. Ye. A1 - Kisilitsyn, R. V. T1 - A 2.44 Ga old mafic dyke swarm in the Kolvitsa Belt, Kola Peninsula, Russia: implications for the early Palaeoproterozoic tectonics in the north-eastern Fennoscandian Shield Y1 - 2001 ER - TY - JOUR A1 - Giest, Hartmut T1 - Lernen und Lehren im entwicklungsfördernden Unterricht Y1 - 2001 ER -