TY - GEN A1 - Bubeck, Philip A1 - Aerts, Jeroen C. J. H. A1 - de Moel, Hans A1 - Kreibich, Heidi T1 - Preface: Flood-risk analysis and integrated management T2 - Natural hazards and earth system sciences Y1 - 2016 U6 - https://doi.org/10.5194/nhess-16-1005-2016 SN - 1561-8633 VL - 16 SP - 1005 EP - 1010 PB - Copernicus CY - Göttingen ER - TY - GEN A1 - Radosavljevic, Boris A1 - Lantuit, Hugues A1 - Pollard, Wayne A1 - Overduin, Pier Paul A1 - Couture, Nicole A1 - Sachs, Torsten A1 - Helm, Veit A1 - Fritz, Michael T1 - Erosion and Flooding - Threats to Coastal Infrastructure in the Arctic: A Case Study from Herschel Island, Yukon Territory, Canada (vol 39, pg 900, 2016) T2 - Estuaries and coasts : journal of the Estuarine Research Federation Y1 - 2016 U6 - https://doi.org/10.1007/s12237-016-0115-z SN - 1559-2723 SN - 1559-2731 VL - 39 SP - 1294 EP - 1295 PB - Springer CY - New York ER - TY - GEN A1 - Cajar, Anke A1 - Engbert, Ralf A1 - Laubrock, Jochen T1 - Eye movements during gaze-contingent spatial-frequency filtering of real-world scenes: Effects of filter location, cutoff, and size T2 - Perception Y1 - 2016 SN - 0301-0066 SN - 1468-4233 VL - 45 SP - 126 EP - 126 PB - Sage Publ. CY - London ER - TY - GEN A1 - Kruegel, Andre A1 - Engbert, Ralf T1 - Statistics of microsaccades indicate early frequency effects during visual word recognition T2 - Perception Y1 - 2016 SN - 0301-0066 SN - 1468-4233 VL - 45 SP - 127 EP - 127 PB - Sage Publ. CY - London ER - TY - GEN A1 - Hanack, Katja A1 - Schloer, Anja A1 - Holzloehner, Pamela A1 - Listek, Martin A1 - Bauer, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Fuener, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk T1 - Camelid nanobodies specific to human pancreatic glycoprotein 2 T2 - The journal of immunology N2 - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease. Y1 - 2016 SN - 0022-1767 SN - 1550-6606 VL - 196 SP - 313 EP - 328 PB - American Assoc. of Immunologists CY - Bethesda ER - TY - GEN A1 - Maier, Natalia A1 - Holzlöhner, Pamela A1 - Hoenow, Anja A1 - Scheunemann, Astrid A1 - Weschke, Daniel A1 - Hanack, Katja T1 - Characterization of monoclonal antibodies generated by in vitro immunization T2 - The journal of immunology N2 - Monoclonal antibodies are highly valuable tools in biomedicine but the generation by hybridoma technology is very time-consuming and elaborate. In order to circumvent the consisting drawbacks an in vitro immunization approach was established by which murine as well as human monoclonal antibodies against a viral coat protein could be developed. The in vitro immunization process was performed by isolation of murine hematopoietic stem cells or human monocytes and an in vitro differentiation into immature dendritic cells. After antigen loading the cells were co-cultivated with naive T and B lymphocytes for three days in order to obtain antigen-specific B lymphocytes in culture, followed by fusion with murine myeloma cells or human/murine heteromyeloma cells. Antigen-specific hybridomas were selected and the generated antibodies were purified and characterized in this study by ELISA, western blot, gene sequencing, affinity measurements. Further the characteristics were compared to a monoclonal antibody against the same target generated by conventional hybridoma technology. Isotype detection revealed a murine IgM and a human IgG4 antibody in comparison to an IgG1 for the conventionally generated antibody. The antibodies derived from in vitro immunization showed indeed a lower affinity for the antigen as compared to the conventionally generated one, which is probably based on the significantly shorter B cell maturation (3 days) during the immunization process. Nevertheless, they were suitable for building up a sandwich based detection system. Therefore, the in vitro immunization approach seems to be a good and particularly fast alternative to conventional hybridoma technology. Y1 - 2016 SN - 0022-1767 SN - 1550-6606 VL - 196 PB - American Assoc. of Immunologists CY - Bethesda ER - TY - GEN A1 - Holzlöhner, Pamela A1 - Butze, Monique A1 - Hebel, Nicole A1 - Weschke, Daniel A1 - Schliebs, E. A1 - Naumann, F. A1 - Füner, J. A1 - Micheel, Burkhard A1 - Hanack, Katja T1 - Monoclonal mouse antibodies against PBMC subpopulations of New World camelides T2 - European journal of immunology Y1 - 2016 SN - 0014-2980 SN - 1521-4141 VL - 46 SP - 1175 EP - 1175 PB - Wiley-Blackwell CY - Hoboken ER - TY - GEN A1 - Lechon, Tamara A1 - Sanz, Luis A1 - Pollmann, Stephan A1 - Sauer, Michael A1 - Sandalio, Luisa A1 - Lorenzo, Oscar T1 - Nitric oxide modification of plant endocytosis and PIN1 localization T2 - New biotechnology Y1 - 2016 U6 - https://doi.org/10.1016/j.nbt.2015.10.028 SN - 1871-6784 SN - 1876-4347 VL - 33 SP - 424 EP - 424 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Hartmann, Matthias A1 - Fischer, Martin H. T1 - Exploring the numerical mind by eye-tracking: a special issue T2 - Psychological research : an international journal of perception, attention, memory, and action Y1 - 2016 U6 - https://doi.org/10.1007/s00426-016-0759-0 SN - 0340-0727 SN - 1430-2772 VL - 80 SP - 325 EP - 333 PB - Springer CY - Heidelberg ER - TY - GEN A1 - Bijleveld, Catrien A1 - Zoutewelle-Terovan, Mioara A1 - Huschek, Doreen A1 - Liefbroer, Aart C. T1 - Criminal careers and demographic outcomes: An introduction to the special issue T2 - Advances in life course research Y1 - 2016 U6 - https://doi.org/10.1016/j.alcr.2016.05.001 SN - 1569-4909 SN - 1879-6974 VL - 28 SP - 1 EP - 5 PB - Elsevier CY - Oxford ER - TY - GEN A1 - Leminen, Alina A1 - Lehtonen, Minna A1 - Bozic, Mirjana A1 - Clahsen, Harald T1 - Editorial: Morphologically Complex Words in the Mind/Brain T2 - Frontiers in human neuroscienc KW - morphology KW - derivation KW - inflection KW - compound KW - L2 KW - dyslexia KW - semantics KW - decomposition Y1 - 2016 U6 - https://doi.org/10.3389/fnhum.2016.00047 SN - 1662-5161 VL - 10 SP - 150 EP - 160 PB - Frontiers Research Foundation CY - Lausanne ER - TY - GEN A1 - Weske, Mathias A1 - Sadiq, Shazia A1 - Soffer, Pnina A1 - Voelzer, Hagen T1 - Preface to BPM 2014 T2 - Information systems Y1 - 2016 U6 - https://doi.org/10.1016/j.is.2015.09.006 SN - 0306-4379 SN - 1873-6076 VL - 56 SP - 233 EP - 234 PB - Elsevier CY - Oxford ER - TY - GEN A1 - Sommer, Michael A1 - Augustin, Jürgen A1 - Kleber, M. T1 - Feedbacks of soil erosion on SOC patterns and carbon dynamics in agricultural landscapes-The CarboZALF experiment T2 - Journal of real-time image processing Y1 - 2016 U6 - https://doi.org/10.1016/j.still.2015.09.015 SN - 0167-1987 SN - 1879-3444 VL - 156 SP - 182 EP - 184 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Lee, J. A1 - Kang, B. A1 - Park, Chan Soo T1 - Effects of the Lifestyle Modification Program on Blood BDNF and Its Associated Factors in Korean Collegiate Students T2 - Research Quarterly for Exercise and Sport Y1 - 2016 SN - 0270-1367 SN - 2168-3824 VL - 87 SP - S113 EP - S113 PB - Freshwater Biological Association CY - Abingdon ER - TY - GEN A1 - Berenstein, Igal A1 - Beta, Carsten A1 - De Decker, Yannick T1 - Comment on "Flow-induced arrest of spatiotemporal chaos and transition to a stationary pattern in the Gray-Scott model" T2 - Physical review : E, Statistical, nonlinear and soft matter physics N2 - In this Comment, we review the results of pattern formation in a reaction-diffusion-advection system following the kinetics of the Gray-Scott model. A recent paper by Das [Phys. Rev. E 92, 052914 (2015)] shows that spatiotemporal chaos of the intermittency type can disappear as the advective flow is increased. This study, however, refers to a single point in the space of kinetic parameters of the original Gray-Scott model. Here we show that the wealth of patterns increases substantially as some of these parameters are changed. In addition to spatiotemporal intermittency, defect-mediated turbulence can also be found. In all cases, however, the chaotic behavior is seen to disappear as the advective flow is increased, following a scenario similar to what was reported in our earlier work [I. Berenstein and C. Beta, Phys. Rev. E 86, 056205 (2012)] as well as by Das. We also point out that a similar phenomenon can be found in other reaction-diffusion-advection models, such as the Oregonator model for the Belousov-Zhabotinsky reaction under flow conditions. Y1 - 2016 U6 - https://doi.org/10.1103/PhysRevE.94.046201 SN - 2470-0045 SN - 2470-0053 VL - 94 PB - American Physical Society CY - College Park ER - TY - GEN A1 - Adak, Hülya T1 - Teaching the Armenian Genocide in Turkey: Curriculum, Methods, and Sources T2 - PMLA : Publications of the Modern Language Association Y1 - 2016 SN - 0030-8129 VL - 131 SP - 1515 EP - 1518 PB - Modern Language Association of America CY - New York ER - TY - GEN A1 - Ehmer, Oliver A1 - Barth-Weingarten, Dagmar T1 - Adverbial patterns in interaction T2 - Language sciences Y1 - 2016 U6 - https://doi.org/10.1016/j.langsci.2016.05.001 SN - 0388-0001 SN - 1873-5746 VL - 58 SP - 1 EP - 7 PB - Elsevier CY - Oxford ER - TY - GEN A1 - Schernthaner, G. A1 - Groop, P. A1 - Cooper, M. A1 - Perkovic, V A1 - Hocher, Berthold A1 - Kanasaki, K. A1 - Sharma, K. A1 - Stanton, R. A1 - Toto, R. A1 - Cescutti, Jessica A1 - Gordat, M. A1 - Meinicke, T. A1 - Koitka-Weber, A. A1 - Woerle, H. A1 - Eynatten, M. T1 - EFFECTS OF LINAGLIPTIN ON GLYCAEMIC CONTROL AND ALBUMINURIA IN TYPE 2 DIABETES - THE MARLINA-T2D (TM) TRIAL T2 - Nephrology Y1 - 2016 U6 - https://doi.org/10.1111/nep.12887 SN - 1320-5358 SN - 1440-1797 VL - 21 SP - 60 EP - 60 PB - Wiley-Blackwell CY - Hoboken ER - TY - GEN A1 - Wutke, Saskia A1 - Andersson, Leif A1 - Benecke, Norbert A1 - Sandoval-Castellanos, Edson A1 - Gonzalez, Javier A1 - Hallsson, Jon Hallsteinn A1 - Lougas, Lembi A1 - Magnell, Ola A1 - Morales-Muniz, Arturo A1 - Orlando, Ludovic A1 - Palsdottir, Albina Hulda A1 - Reissmann, Monika A1 - Munoz-Rodriguez, Mariana B. A1 - Ruttkay, Matej A1 - Trinks, Alexandra A1 - Hofreiter, Michael A1 - Ludwig, Arne T1 - The origin of ambling horses T2 - Current biology N2 - Horseback riding is the most fundamental use of domestic horses and has had a huge influence on the development of human societies for millennia. Over time, riding techniques and the style of riding improved. Therefore, horses with the ability to perform comfortable gaits (e.g. ambling or pacing), so-called ‘gaited’ horses, have been highly valued by humans, especially for long distance travel. Recently, the causative mutation for gaitedness in horses has been linked to a substitution causing a premature stop codon in the DMRT3 gene (DMRT3_Ser301STOP) [1]. In mice, Dmrt3 is expressed in spinal cord interneurons and plays an important role in the development of limb movement coordination [1]. Genotyping the position in 4396 modern horses from 141 breeds revealed that nowadays the mutated allele is distributed worldwide with an especially high frequency in gaited horses and breeds used for harness racing [2]. Here, we examine historic horse remains for the DMRT3 SNP, tracking the origin of gaitedness to Medieval England between 850 and 900 AD. The presence of the corresponding allele in Icelandic horses (9th–11th century) strongly suggests that ambling horses were brought from the British Isles to Iceland by Norse people. Considering the high frequency of the ambling allele in early Icelandic horses, we believe that Norse settlers selected for this comfortable mode of horse riding soon after arrival. The absence of the allele in samples from continental Europe (including Scandinavia) at this time implies that ambling horses may have spread from Iceland and maybe also the British Isles across the continent at a later date. Y1 - 2016 U6 - https://doi.org/10.1016/j.cub.2016.07.001 SN - 0960-9822 SN - 1879-0445 VL - 26 SP - R697 EP - R699 PB - Cell Press CY - Cambridge ER - TY - GEN A1 - Völler, Heinz A1 - Eichler, Sarah A1 - Harnath, A. A1 - Nothroff, Jörg A1 - Butter, Christian A1 - Schikora, Martin A1 - Wegscheider, Karl A1 - Salzwedel, Annett T1 - Multicomponent cardiac rehabilitation in patients after transcatheter aortic valve implantation (TAVI) - course of functioning and quality of life T2 - European heart journal Y1 - 2016 SN - 0195-668X SN - 1522-9645 VL - 37 SP - 542 EP - 542 PB - Oxford Univ. Press CY - Oxford ER -