TY - JOUR A1 - Putra, Sulistyo Emantoko Dwi A1 - Tsuprykov, Oleg A1 - Von Websky, Karoline A1 - Ritter, Teresa A1 - Reichetzeder, Christoph A1 - Hocher, Berthold T1 - Dealing with large sample sizes: comparison of a new one spot dot blot method to western blot JF - Clinical laboratory : the peer reviewed journal for clinical laboratories and laboratories related to blood transfusion N2 - Background: Western blot is the gold standard method to determine individual protein expression levels. However, western blot is technically difficult to perform in large sample sizes because it is a time consuming and labor intensive process. Dot blot is often used instead when dealing with large sample sizes, but the main disadvantage of the existing dot blot techniques, is the absence of signal normalization to a housekeeping protein. Methods: In this study we established a one dot two development signals (ODTDS) dot blot method employing two different signal development systems. The first signal from the protein of interest was detected by horseradish peroxidase (HRP). The second signal, detecting the housekeeping protein, was obtained by using alkaline phosphatase (AP). Results: Inter-assay results variations within ODTDS dot blot and western blot and intra-assay variations between both methods were low (1.04 - 5.71%) as assessed by coefficient of variation. Conclusions: ODTDS dot blot technique can be used instead of western blot when dealing with large sample sizes without a reduction in results accuracy. KW - one dot two development signals (ODTDS) dot blot KW - western blot KW - protein quantification KW - large sample size studies KW - comparison Y1 - 2014 U6 - https://doi.org/10.7754/Clin.Lab.2014.140317 SN - 1433-6510 VL - 60 IS - 11 SP - 1871 EP - 1877 PB - Clin Lab Publ., Verl. Klinisches Labor CY - Heidelberg ER - TY - GEN A1 - Kuhlmann, Sabine A1 - Wayenberg, Ellen T1 - Institutional impact assessment in multi-level systems BT - conceptualizing decentralization effects from a comparative perspective T2 - International review of administrative sciences N2 - Comparative literature on institutional reforms in multi-level systems proceeds from a global trend towards the decentralization of state functions. However, there is only scarce knowledge about the impact that decentralization has had, in particular, upon the sub-central governments involved. How does it affect regional and local governments? Do these reforms also have unintended outcomes on the sub-central level and how can this be explained? This article aims to develop a conceptual framework to assess the impacts of decentralization on the sub-central level from a comparative and policyoriented perspective. This framework is intended to outline the major patterns and models of decentralization and the theoretical assumptions regarding de-/re-centralization impacts, as well as pertinent cross-country approaches meant to evaluate and compare institutional reforms. It will also serve as an analytical guideline and a structural basis for all the country-related articles in this Special Issue. T3 - Zweitveröffentlichungen der Universität Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe - 91 KW - administrative reform KW - comparison KW - coordination KW - effectiveness KW - efficiency KW - impact assessment KW - institutional reform, KW - local government Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-405314 VL - 82 IS - 2 ER - TY - JOUR A1 - Kuhlmann, Sabine A1 - Wayenberg, Ellen T1 - Institutional impact assessment in multi-level systems: conceptualizing decentralization effects from a comparative perspective JF - International review of administrative sciences : an international journal of comparative public administration N2 - Comparative literature on institutional reforms in multi-level systems proceeds from a global trend towards the decentralization of state functions. However, there is only scarce knowledge about the impact that decentralization has had, in particular, upon the sub-central governments involved. How does it affect regional and local governments? Do these reforms also have unintended outcomes on the sub-central level and how can this be explained? This article aims to develop a conceptual framework to assess the impacts of decentralization on the sub-central level from a comparative and policy-oriented perspective. This framework is intended to outline the major patterns and models of decentralization and the theoretical assumptions regarding de-/re-centralization impacts, as well as pertinent cross-country approaches meant to evaluate and compare institutional reforms. It will also serve as an analytical guideline and a structural basis for all the country-related articles in this Special Issue. Points for practitioners Decentralization reforms are approved as having a key role to play in the attainment of ‘good governance’. Yet, there is also the enticement on the part of state governments to offload an ever-increasing amount of responsibilities to, and overtask, local levels of government, which can lead to increasing performance disparities within local sub-state jurisdictions. Against this background, the article provides a conceptual framework to assess reform impacts from a comparative perspective. The analytical framework can be used by practitioners to support their decisions about new decentralization strategies or necessary adjustments regarding ongoing reform measures. KW - administrative reform KW - comparison KW - coordination KW - effectiveness KW - efficiency KW - impact assessment KW - institutional reform KW - local government Y1 - 2016 U6 - https://doi.org/10.1177/0020852315583194 SN - 0020-8523 SN - 1461-7226 VL - 82 IS - 2 SP - 233 EP - 272 PB - Sage CY - London ER - TY - GEN A1 - Welearegai, Gebrehiwet B. A1 - Schlueter, Max A1 - Hammer, Christian T1 - Static security evaluation of an industrial web application T2 - Proceedings of the 34th ACM/SIGAPP Symposium on Applied Computing N2 - JavaScript is the most popular programming language for web applications. Static analysis of JavaScript applications is highly challenging due to its dynamic language constructs and event-driven asynchronous executions, which also give rise to many security-related bugs. Several static analysis tools to detect such bugs exist, however, research has not yet reported much on the precision and scalability trade-off of these analyzers. As a further obstacle, JavaScript programs structured in Node. js modules need to be collected for analysis, but existing bundlers are either specific to their respective analysis tools or not particularly suitable for static analysis. KW - JavaScript KW - WALA KW - SAFE KW - comparison Y1 - 2019 SN - 978-1-4503-5933-7 U6 - https://doi.org/10.1145/3297280.3297471 SP - 1952 EP - 1961 PB - Association for Computing Machinery CY - New York ER - TY - JOUR A1 - Bangeow, Petjo A1 - Rapp, Michael Armin T1 - Beurteilung und Nutzung der 2016 reformierten Psychotherapierichtlinie T1 - Assessment and use of 2016 reformed psychotherapeutic guideline BT - Teil II : Ost-West- und Stadt-Land-Vergleich BT - part II : comparing Eastern and Western Germany as well as urban and rural areas JF - Psychiatrische Praxis : Sozialpsychiatrie, klinische Psychiatrie, public mental health, Versorgungsforschung N2 - Ziel der Studie Dieser Artikel untersucht, inwiefern sich die 2016 reformierte Richtlinie im Stadt-Land- sowie im Ost-West-Vergleich auf die ambulante psychotherapeutische Arbeit und Versorgung auswirkt. Methodik Eine Onlineumfrage unter vertragsärztlich tätigen TherapeutInnen wurde durchgeführt. Die Fragen bezogen sich auf verschiedene Neuerungen in der Richtlinie. Ergebnisse Unabhängig von der Region schätzten die Befragten ein, dass die Reform zu keiner verbesserten Versorgung führte. Im Westen und in der Stadt tätige TherapeutInnen verwiesen PatientInnen nach der Sprechstunde öfter an andere Psychotherapiepraxen, im Osten und auf dem Land tätige hingegen öfter auf andere Hilfeangebote. Schlussfolgerung Stärkere Anreize für die psychotherapeutische Tätigkeit auf dem Land sind zu schaffen. Abbaumaßnahmen der Ost-West-Ungleichheiten in der Versorgungsdichte scheinen nötig. N2 - Objective This article investigated whether or not the reformed psychotherapeutic guideline had different effects on outpatient psychotherapeutic work and services comparing urban and rural areas as well as eastern and western Germany. Methods An online survey of psychotherapists was conducted. The survey included questions on various innovations within the reformed guideline. Results Regardless of region, therapists stated overall that the reform has not led to improved psychotherapeutic care. Therapists in the West and in urban areas referred more patients to other psychotherapeutic practices after their consultation. Therapists in the East and in rural areas referred more patients to other institutions and services. Conclusions Psychotherapeutic work in rural areas should be better incentivized. Interventions to reduce east-west inequalities in the density of service provision seem to be necessary. KW - Psychotherapierichtlinie KW - Ostdeutschland KW - Westdeutschland KW - Stadt-Land-Vergleich KW - psychotherapy guidelines KW - Eastern-Western Germany KW - urban-rural KW - comparison Y1 - 2020 U6 - https://doi.org/10.1055/a-1045-9820 SN - 0303-4259 SN - 1439-0876 VL - 47 IS - 07 SP - 383 EP - 387 PB - Thieme CY - Stuttgart ER - TY - JOUR A1 - Levy, Jessica A1 - Mussack, Dominic A1 - Brunner, Martin A1 - Keller, Ulrich A1 - Cardoso-Leite, Pedro A1 - Fischbach, Antoine T1 - Contrasting classical and machine learning approaches in the estimation of value-added scores in large-scale educational data JF - Frontiers in psychology N2 - There is no consensus on which statistical model estimates school value-added (VA) most accurately. To date, the two most common statistical models used for the calculation of VA scores are two classical methods: linear regression and multilevel models. These models have the advantage of being relatively transparent and thus understandable for most researchers and practitioners. However, these statistical models are bound to certain assumptions (e.g., linearity) that might limit their prediction accuracy. Machine learning methods, which have yielded spectacular results in numerous fields, may be a valuable alternative to these classical models. Although big data is not new in general, it is relatively new in the realm of social sciences and education. New types of data require new data analytical approaches. Such techniques have already evolved in fields with a long tradition in crunching big data (e.g., gene technology). The objective of the present paper is to competently apply these "imported" techniques to education data, more precisely VA scores, and assess when and how they can extend or replace the classical psychometrics toolbox. The different models include linear and non-linear methods and extend classical models with the most commonly used machine learning methods (i.e., random forest, neural networks, support vector machines, and boosting). We used representative data of 3,026 students in 153 schools who took part in the standardized achievement tests of the Luxembourg School Monitoring Program in grades 1 and 3. Multilevel models outperformed classical linear and polynomial regressions, as well as different machine learning models. However, it could be observed that across all schools, school VA scores from different model types correlated highly. Yet, the percentage of disagreements as compared to multilevel models was not trivial and real-life implications for individual schools may still be dramatic depending on the model type used. Implications of these results and possible ethical concerns regarding the use of machine learning methods for decision-making in education are discussed. KW - value-added modeling KW - school effectiveness KW - machine learning KW - model KW - comparison KW - longitudinal data Y1 - 2020 U6 - https://doi.org/10.3389/fpsyg.2020.02190 SN - 1664-1078 VL - 11 PB - Frontiers Research Foundation CY - Lausanne ER - TY - JOUR A1 - Kuhlmann, Sabine A1 - Bouckaert, Geert A1 - Galli, Davide A1 - Reiter, Renate A1 - van Hecke, Steven T1 - Opportunity management of the COVID-19 pandemic BT - testing the crisis from a global perspective JF - International review of administrative sciences N2 - This article provides a conceptual framework for the analysis of COVID-19 crisis governance in the first half of 2020 from a cross-country comparative perspective. It focuses on the issue of opportunity management, that is, how the crisis was used by relevant actors of distinctly different administrative cultures as a window of opportunity. We started from an overall interest in the factors that have influenced the national politics of crisis management to answer the question of whether and how political and administrative actors in various countries have used the crisis as an opportunity to facilitate, accelerate or prevent changes in institutional settings. The objective is to study the institutional settings and governance structures, (alleged) solutions and remedies, and constellations of actors and preferences that have influenced the mode of crisis and opportunity management. Finally, the article summarizes some major comparative findings drawn from the country studies of this Special Issue, focusing on similarities and differences in crisis responses and patterns of opportunity management. KW - administrative culture KW - comparison KW - COVID-19 KW - crisis management KW - governance KW - opportunity management KW - pandemic KW - window of opportunity Y1 - 2021 U6 - https://doi.org/10.1177/0020852321992102 SN - 0020-8523 SN - 1461-7226 VL - 87 IS - 3 SP - 497 EP - 517 PB - Sage CY - Los Angeles, California ER - TY - JOUR A1 - Kindermann, Liana A1 - Dobler, Magnus A1 - Niedeggen, Daniela A1 - Linstädter, Anja T1 - A new protocol for estimation of woody aboveground biomass in disturbance-prone ecosystems JF - Ecological indicators : integrating monitoring, assessment and management N2 - Almost one third of global drylands are open forests and savannas, which are typically shaped by frequent natural disturbances such as wildfire and herbivory. Studies on ecosystem functions and services of woody vegetation require robust estimates of aboveground biomass (AGB). However, most methods have been developed for comparatively undisturbed forest ecosystems. As they are not tailored to accurately quantify AGB of small and irregular growth forms, their application on these growth forms may lead to unreliable or even biased AGB estimates in disturbance-prone dryland ecosystems. Moreover, these methods cannot quantify AGB losses caused by disturbance agents. Here we propose a methodology to estimate individual-and stand-level woody AGB in disturbance-prone ecosystems. It consists of flexible field sampling routines and estimation workflows for six growth classes, delineated by size and damage criteria. It also comprises a detailed damage assessment, harnessing the ecological archive of woody growth for past disturbances. Based on large inventories collected along steep gradients of elephant disturbances in African dryland ecosystems, we compared the AGB estimates generated with our proposed method against estimates from a less adapted forest inventory method. We evaluated the necessary stepwise procedures of method adaptation and analyzed each step's effect on stand-level AGB estimation. We further explored additional advantages of our proposed method with regard to disturbance impact quantification. Results indicate that a majority of growth forms and individuals in savanna vegetation could only be assessed if methods of AGB estimation were adapted to the conditions of a disturbance-prone ecosystem. Furthermore, our damage assessment demonstrated that one third to half of all woody AGB was lost to disturbances. Consequently, less adapted methods may be insufficient and are likely to render inaccurate AGB estimations. Our proposed method has the potential to accurately quantify woody AGB in disturbance-prone ecosystems, as well as AGB losses. Our method is more time consuming than conventional allometric approaches, yet it can cover sufficient areas within reasonable timespans, and can also be easily adapted to alternative sampling schemes. KW - Damage assessment KW - Disturbance impacts KW - Tree growth classes KW - Method KW - comparison KW - Flexible sampling strategy KW - Tree allometry KW - Woody KW - aboveground biomass Y1 - 2021 U6 - https://doi.org/10.1016/j.ecolind.2021.108466 SN - 1470-160X SN - 1872-7034 VL - 135 PB - Elsevier CY - Amsterdam ER - TY - BOOK ED - Bogumil, Jörg ED - Kuhlmann, Sabine ED - Hafner, Jonas ED - Kastilan, André ED - Oehlert, Franziska ED - Reusch, Marie Catherine T1 - Lokales Integrationsmanagement in Deutschland, Schweden und Frankreich N2 - Im vorliegenden Band wird das lokale Integrationsmanagement in Deutschland, Frankreich und Schweden vergleichend untersucht. Im Mittelpunkt stehen Verflechtungsstrukturen, Koordination und Leistungsfähigkeit der Integrationsverwaltung mit besonderem Fokus auf den Entwicklungen nach der Flüchtlingskrise von 2015/16. Auf der Grundlage von Fallstudien und Experteninterviews in den drei Ländern wird das institutionelle Zusammenspiel von Akteuren im Mehrebenensystem und im lokalen Raum analysiert. Dabei werden jeweils die nationalen Rahmenbedingungen, lokalen Gestaltungsvarianten und krisenbedingten Herausforderungen des Integrationsmanagement kommunen- und ländervergleichend in den Blick genommen. Gestützt auf illustrative Praxisbeispiele und Tiefeneinblicke in die lokalen Handlungsprobleme leitet die Studie Lehren und Empfehlungen für eine Optimierung des Integrationsmanagements und eine krisenresilientere Verwaltungsorganisation in diesem Aufgabenbereich ab. N2 - This study analyses local integration management in Germany, France and Sweden from a comparative perspective. It focuses on the inter-administrative relations, coordination and performance of integration management, with a particular focus on developments after the refugee crisis of 2015/16. Based on case studies and expert interviews in the aforementioned three countries, it analyses the institutional interplay between actors in both the multi-level system and the local sphere. The authors examine the national contexts, local institutional settings and crisis-related challenges of integration management performance in the three countries, taking similarities and differences from a cross-country and inter-municipal com-parative perspective into account. Using illustrative examples from practice and deriving lessons from in-depth insights into local problem-solving, the study makes recommendations for the optimisation of integration management and more crisis-resilient administrative organisation in this policy area. KW - Deutschland KW - Flüchtlingskrise KW - Frankreich KW - Gestaltungsvarianten KW - Integration KW - Integrationsmanagement KW - Koordination KW - Krisenresilienz KW - Mehrebenensystem KW - Optimierung KW - Rahmenbedingungen KW - Schweden KW - Vergleich KW - Verwaltung KW - Verwaltungsorganisation KW - administration KW - administrative organization KW - comparison KW - coordination KW - crisis resilience KW - design variants KW - framework conditions KW - France KW - integration KW - integration management KW - multi-level system KW - optimization KW - refugee crisis KW - Sweden KW - Germany Y1 - 2023 SN - 978-3-7560-0558-1 SN - 978-3-7489-3911-5 U6 - https://doi.org/10.5771/9783748939115 PB - Nomos CY - Baden-Baden ER -