TY  - THES
A1  - Lischke, Timo
T1  - Functional correlates of CD4+ cell tolerance and immunity to soluble protein antigen in vivo
Y1  - 2009
SN  - 978-3-89432-116-1
PB  - Westarp Wissenschaften
CY  - Hohenwarsleben
ER  - 
TY  - JOUR
A1  - Kobel-Höller, Konstanze
A1  - Gley, Kevin
A1  - Jochinke, Janina
A1  - Heider, Kristina
A1  - Fritsch, Verena Nadin
A1  - Ha Viet Duc Nguyen, 
A1  - Lischke, Timo
A1  - Radek, Renate
A1  - Baumgrass, Ria
A1  - Mutzel, Rupert
A1  - Thewes, Sascha
T1  - Calcineurin Silencing in Dictyostelium discoideum Leads to Cellular Alterations Affecting Mitochondria, Gene Expression, and Oxidative Stress Response
JF  - Protist
N2  - Calcineurin is involved in development and cell differentiation of the social amoeba Dictyostelium discoideum. However, since knockouts of the calcineurin-encoding genes are not possible in D. discoideum it is assumed that the phosphatase also plays a crucial role during vegetative growth of the amoebae. Therefore, we investigated the role of calcineurin during vegetative growth in D. discoideum. RNAi-silenced calcineurin mutants showed cellular alterations with an abnormal morphology of mitochondria and had increased content of mitochondrial DNA (mtDNA). In contrast, mitochondria showed no substantial functional impairment. Calcineurin-silencing led to altered expression of calcium-regulated genes as well as mitochondrially-encoded genes. Furthermore, genes related to oxidative stress were higher expressed in the mutants, which correlated to an increased resistance towards reactive oxygen species (ROS). Most of the changes observed during vegetative growth were not seen after starvation of the calcineurin mutants. We show that impairment of calcineurin led to many subtle, but in the sum crucial cellular alterations in vegetative D. discoideum cells. As these alterations were not observed after starvation we propose a dual role for calcineurin during growth and development. Our results imply that calcineurin is one player in the mutual interplay between mitochondria and ROS during vegetative growth.
KW  - mtDNA
KW  - mitochondrial remodelling
KW  - calcium
KW  - oxidative stress
KW  - phototaxis
Y1  - 2018
U6  - https://doi.org/10.1016/j.protis.2018.04.004
SN  - 1434-4610
VL  - 169
IS  - 4
SP  - 584
EP  - 602
PB  - Elsevier GMBH
CY  - München
ER  - 
TY  - JOUR
A1  - Gryzik, Stefanie
A1  - Hoang, Yen
A1  - Lischke, Timo
A1  - Mohr, Elodie
A1  - Venzke, Melanie
A1  - Kadner, Isabelle
A1  - Pötzsch, Josephine
A1  - Groth, Detlef
A1  - Radbruch, Andreas
A1  - Hutloff, Andreas
A1  - Baumgrass, Ria
T1  - Identification of a super-functional Tfh-like subpopulation in murine lupus by pattern perception
JF  - eLife
N2  - Dysregulated cytokine expression by T cells plays a pivotal role in the pathogenesis of autoimmune diseases. However, the identification of the corresponding pathogenic subpopulations is a challenge, since a distinction between physiological variation and a new quality in the expression of protein markers requires combinatorial evaluation. Here, we were able to identify a super-functional follicular helper T cell (Tfh)-like subpopulation in lupus-prone NZBxW mice with our binning approach "pattern recognition of immune cells (PRI)". PRI uncovered a subpopulation of IL-21(+) IFN-gamma(high) PD-1(low) CD40L(high) CXCR5(-) Bcl-6(-) T cells specifically expanded in diseased mice. In addition, these cells express high levels of TNF-alpha and IL-2, and provide B cell help for IgG production in an IL-21 and CD40L dependent manner. This super-functional T cell subset might be a superior driver of autoimmune processes due to a polyfunctional and high cytokine expression combined with Tfh-like properties.
Y1  - 2020
U6  - https://doi.org/10.7554/eLife.53226
SN  - 2050-084X
VL  - 9
PB  - eLife Sciences Publications
CY  - Cambridge
ER  -