TY  - JOUR
A1  - Zivanovic, Vesna
A1  - Kochovski, Zdravko
A1  - Arenz, Christoph
A1  - Lu, Yan
A1  - Kneipp, Janina
T1  - SERS and Cryo-EM Directly Reveal Different Liposome Structures during Interaction with Gold Nanoparticles
JF  - The journal of physical chemistry letters
N2  - The combination of gold nanoparticles with liposomes is important for nano- and biotechnology. Here, we present direct, label-free characterization of liposome structure and composition at the site of its interaction with citrate-stabilized gold nanoparticles by surface-enhanced Raman scattering (SERS) and cryogenic electron microscopy (cryo-EM). Evidenced by the vibrational spectra and cryo-EM, the gold nanoparticles destroy the bilayer structure of interacting liposomes in the presence of a high amount of citrate, while at lower citrate concentration the nanoparticles interact with the surface of the intact liposomes. The spectra of phosphatidylcholine and phosphatidylcholine/sphingomyelin liposomes show that at the site of interaction the lipid chains are in the gel phase. The SERS spectra indicate that cholesterol has strong effects on the contacts of the vesicles with the nanoparticles. By combining cryo-EM and SERS, the structure and properties of lipid nanoparticle composites could be tailored for the development of drug delivery systems.
Y1  - 2018
U6  - https://doi.org/10.1021/acs.jpclett.8b03191
SN  - 1948-7185
VL  - 9
IS  - 23
SP  - 6767
EP  - 6772
PB  - American Chemical Society
CY  - Washington
ER  -