TY - JOUR A1 - Couturier, Jean-Philippe A1 - Sütterlin, Martin A1 - Laschewsky, André A1 - Hettrich, Cornelia A1 - Wischerhoff, Erik T1 - Responsive Inverse Opal Hydrogels for the Sensing of Macromolecules JF - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition N2 - Dual responsive inverse opal hydrogels were designed as autonomous sensor systems for (bio)macromolecules, exploiting the analyte-induced modulation of the opal's structural color. The systems that are based on oligo(ethylene glycol) macromonomers additionally incorporate comonomers with various recognition units. They combine a coil-to-globule collapse transition of the LCST type with sensitivity of the transition temperature toward molecular recognition processes. This enables the specific detection of macromolecular analytes, such as glycopolymers and proteins, by simple optical methods. While the inverse opal structure assists the effective diffusion even of large analytes into the photonic crystal, the stimulus responsiveness gives rise to strong shifts of the optical Bragg peak of more than 100nm upon analyte binding at a given temperature. The systems' design provides a versatile platform for the development of easy-to-use, fast, and low-cost sensors for pathogens. KW - hydrogels KW - photonic crystals KW - polymers KW - responsive materials KW - sensors Y1 - 2015 U6 - https://doi.org/10.1002/anie.201500674 SN - 1433-7851 SN - 1521-3773 VL - 54 IS - 22 SP - 6641 EP - 6644 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Schwarze, Thomas A1 - Kelling, Alexandra A1 - Müller, Holger A1 - Trautmann, Michael A1 - Klamroth, Tillmann A1 - Baumann, Otto A1 - Strauch, Peter A1 - Holdt, Hans-Jürgen T1 - N-2-Pyridinylmethyl-N '-arylmethyl-diaminomaleonitriles: New Highly Selective Chromogenic Chemodosimeters for Copper(II) JF - Chemistry - a European journal KW - amides KW - chemodosimeter KW - colorimetric detection KW - copper KW - sensors KW - UV KW - Vis spectroscopy Y1 - 2012 U6 - https://doi.org/10.1002/chem.201201731 SN - 0947-6539 VL - 18 IS - 34 SP - 10506 EP - 10510 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Schwarze, Thomas A1 - Müller, Holger A1 - Ast, Sandra A1 - Steinbrück, Dörte A1 - Eidner, Sascha A1 - Geißler, Felix A1 - Kumke, Michael Uwe A1 - Holdt, Hans-Jürgen ED - Kumke, Michael Uwe T1 - Fluorescence lifetime-based sensing of sodium by an optode JF - Chemical Communications N2 - We report a 1,2,3-triazol fluoroionophore for detecting Na+ that shows in vitro enhancement in the Na+-induced fluorescence intensity and decay time. The Na+-selective molecule 1 was incorporated into a hydrogel as a part of a fiber optical sensor. This sensor allows the direct determination of Na+ in the range of 1–10 mM by measuring reversible fluorescence decay time changes. KW - ion optodes KW - sensors KW - indicators KW - chromoionophore KW - ionophore KW - membrane KW - switches KW - systems KW - samples KW - green Y1 - 2014 SN - 0022-4936 SN - 0009-241X SP - 14167 EP - 14170 PB - The Royal Society Chemistry CY - Cambridge ER - TY - GEN A1 - Schwarze, Thomas A1 - Müller, Holger A1 - Ast, Sandra A1 - Steinbrück, Dörte A1 - Eidner, Sascha A1 - Geißler, Felix A1 - Kumke, Michael Uwe A1 - Holdt, Hans-Jürgen T1 - Fluorescence lifetime-based sensing of sodium by an optode N2 - We report a 1,2,3-triazol fluoroionophore for detecting Na+ that shows in vitro enhancement in the Na+-induced fluorescence intensity and decay time. The Na+-selective molecule 1 was incorporated into a hydrogel as a part of a fiber optical sensor. This sensor allows the direct determination of Na+ in the range of 1–10 mM by measuring reversible fluorescence decay time changes. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 182 KW - ion optodes KW - sensors KW - indicators KW - chromoionophore KW - ionophore KW - membrane KW - switches KW - systems KW - samples KW - green Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-76785 SP - 14167 EP - 14170 PB - The Royal Society of Chemistry CY - Cambridge ER - TY - JOUR A1 - Ast, Sandra A1 - Schwarze, Thomas A1 - Müller, Holger A1 - Sukhanov, Aleksey A1 - Michaelis, Stefanie A1 - Wegener, Joachim A1 - Wolfbeis, Otto S. A1 - Körzdörfer, Thomas A1 - Dürkop, Axel A1 - Holdt, Hans-Jürgen T1 - A highly K+-Selective Phenylaza-[18]crown-6-Lariat-Ether-Based Fluoroionophore and its application in the sensing of K+ Ions with an optical sensor film and in cells JF - Chemistry - a European journal N2 - Herein, we report the synthesis of two phenylaza-[18]crown-6 lariat ethers with a coumarin fluorophore (1 and 2) and we reveal that compound 1 is an excellent probe for K+ ions under simulated physiological conditions. The presence of a 2-methoxyethoxy lariat group at the ortho position of the anilino moiety is crucial to the substantially increased stability of compounds 1 and 2 over their lariat-free phenylaza-[18] crown-6 ether analogues. Probe 1 shows a high K+/Na+ selectivity and a 2.5-fold fluorescence enhancement was observed in the presence of 100 mm K+ ions. A fluorescent membrane sensor, which was prepared by incorporating probe 1 into a hydrogel, showed a fully reversible response, a response time of 150 s, and a signal change of 7.8% per 1 mm K+ within the range 1-10 mm K+. The membrane was easily fabricated (only a single sensing layer on a solid polyester support), yet no leaching was observed. Moreover, compound 1 rapidly permeated into cells, was cytocompatible, and was suitable for the fluorescent imaging of K+ ions on both the extracellular and intracellular levels. KW - crown compounds KW - fluorescence KW - gels KW - potassium KW - sensors Y1 - 2013 U6 - https://doi.org/10.1002/chem.201302350 SN - 0947-6539 SN - 1521-3765 VL - 19 IS - 44 SP - 14911 EP - 14917 PB - Wiley-VCH CY - Weinheim ER -