TY  - JOUR
A1  - Mertens, Monique
A1  - Hilsch, Malte
A1  - Haralampiev, Ivan
A1  - Volkmer, Rudolf
A1  - Wessig, Pablo
A1  - Müller, Peter
T1  - Synthesis and characterization of a new Bifunctionalized, Fluorescent, and Amphiphilic molecule for recruiting SH-Containing molecules to membranes
JF  - ChemBioChem
N2  - This study describes the synthesis and characterization of an amphiphilic construct intended to recruit SH-containing molecules to membranes. The construct consists of 1)an aliphatic chain to enable anchoring within membranes, 2)a maleimide moiety to react with the sulfhydryl group of a soluble (bio)molecule, and 3)a fluorescence moiety to allow the construct to be followed by fluorescence spectroscopy and microscopy. It is shown that the construct can be incorporated into preformed membranes, thus allowing application of the approach with biological membranes. The close proximity between the fluorophore and the maleimide moiety within the construct causes fluorescence quenching. This allows monitoring of the reaction with SH-containing molecules by measurement of increases in fluorescence intensity and lifetime. Notably, the construct distributes into laterally ordered membrane domains of lipid vesicles, which is probably triggered by the length of its membrane anchor. The advantages of the new construct can be employed for several biological, biotechnological, and medicinal applications.
KW  - DBD dyes
KW  - fatty acids
KW  - liposomes
KW  - maleimide
KW  - membranes
KW  - palmitoylation
Y1  - 2018
U6  - https://doi.org/10.1002/cbic.201800268
SN  - 1439-4227
SN  - 1439-7633
VL  - 19
IS  - 15
SP  - 1643
EP  - 1647
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Haralampiev, Ivan
A1  - Mertens, Monique
A1  - Schwarzer, Roland
A1  - Herrmann, Andreas
A1  - Volkmer, Rudolf
A1  - Wessig, Pablo
A1  - Mueller, Peter
T1  - Recruitment of SH-Containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a Maleimide Group
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.
KW  - liposomes
KW  - maleimide
KW  - membranes
KW  - palmitic acid
KW  - palmitoylation
KW  - peptides
Y1  - 2015
U6  - https://doi.org/10.1002/anie.201408089
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 1
SP  - 323
EP  - 326
PB  - Wiley-VCH
CY  - Weinheim
ER  -